il 23  (R&D Systems)

 
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    Name:
    Recombinant Human IL 23 Protein CF
    Description:
    The Recombinant Human IL 23 Protein from R D Systems is derived from Sf 21 baculovirus The Recombinant Human IL 23 Protein has been validated for the following applications Bioactivity
    Catalog Number:
    1290-IL-010/CF
    Price:
    329
    Category:
    Proteins and Enzymes
    Source:
    Sf 21 (baculovirus)-derived Recombinant Human IL-23 Protein
    Applications:
    Bioactivity
    Purity:
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
    Conjugate:
    Unconjugated
    Size:
    10 ug
    Buy from Supplier


    Structured Review

    R&D Systems il 23
    Rank sum test analysis of the relationship between the balance of 2 cytokines, soluble interleukin‐23 receptor (sIL‐23R) and <t>IL‐23,</t> and N stage (A), T stage (B) and IL‐17 (C) of non‐small cell lung cancer patients. The ordinate was the rank mean. * P
    The Recombinant Human IL 23 Protein from R D Systems is derived from Sf 21 baculovirus The Recombinant Human IL 23 Protein has been validated for the following applications Bioactivity
    https://www.bioz.com/result/il 23/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 23 - by Bioz Stars, 2021-05
    99/100 stars

    Images

    1) Product Images from "Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma, et al. Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma"

    Article Title: Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma, et al. Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma

    Journal: Cancer Science

    doi: 10.1111/cas.14343

    Rank sum test analysis of the relationship between the balance of 2 cytokines, soluble interleukin‐23 receptor (sIL‐23R) and IL‐23, and N stage (A), T stage (B) and IL‐17 (C) of non‐small cell lung cancer patients. The ordinate was the rank mean. * P
    Figure Legend Snippet: Rank sum test analysis of the relationship between the balance of 2 cytokines, soluble interleukin‐23 receptor (sIL‐23R) and IL‐23, and N stage (A), T stage (B) and IL‐17 (C) of non‐small cell lung cancer patients. The ordinate was the rank mean. * P

    Techniques Used:

    A, Kaplan‐Meier analysis of serum soluble interleukin‐23 receptor (sIL‐23R) for non‐small cell lung cancer (NSCLC) patient prognosis. B, Merged survival curves of NSCLC patient subgroups with high ( > 2.56), intermediate (0.43‐2.56), and low (
    Figure Legend Snippet: A, Kaplan‐Meier analysis of serum soluble interleukin‐23 receptor (sIL‐23R) for non‐small cell lung cancer (NSCLC) patient prognosis. B, Merged survival curves of NSCLC patient subgroups with high ( > 2.56), intermediate (0.43‐2.56), and low (

    Techniques Used:

    Identification of soluble interleukin‐23 receptor (sIL‐23R) in non‐small cell lung cancer (NSCLC) and healthy control serum samples. A, Gel was visualized by silver staining. The mass spectroscopy (MS) results were analyzed after removing chemical and electrical signal noises, and through a series of processing such as data correction and normalization. Left panels, normal control; right panels, NSCLC. Red arrow in the box indicates the discrepant masses corresponding to sIL‐23R. B, The protein spot with discrepancies between healthy controls and NSCLC patients was analyzed using MALDI time of flight (TOF)/TOF MS. C, sIL‐23R fragments showed 12.2% amino acid sequence coverage in the MS analysis
    Figure Legend Snippet: Identification of soluble interleukin‐23 receptor (sIL‐23R) in non‐small cell lung cancer (NSCLC) and healthy control serum samples. A, Gel was visualized by silver staining. The mass spectroscopy (MS) results were analyzed after removing chemical and electrical signal noises, and through a series of processing such as data correction and normalization. Left panels, normal control; right panels, NSCLC. Red arrow in the box indicates the discrepant masses corresponding to sIL‐23R. B, The protein spot with discrepancies between healthy controls and NSCLC patients was analyzed using MALDI time of flight (TOF)/TOF MS. C, sIL‐23R fragments showed 12.2% amino acid sequence coverage in the MS analysis

    Techniques Used: Silver Staining, Mass Spectrometry, Sequencing

    A, Relative expression of interleukin‐23 receptor (IL‐23R) in lung adenocarcinoma cell line A549, lung squamous carcinoma cell line H226, and lung immortalized cell line 16HBE. Original mean value of relative expression of 16HBE was 3.84 × 10 −6 . For simplification, we set the value of 16HBE as 1, and the other values of cell lines and tissues were corrected by the baseline. B, Each pair of data points connected by a line represent the relative expression of IL‐23R from tumor and adjacent noncancerous tissues of a single donor. Black line with gray block next to each group indicates median and 25%‐75% percentile. *** P
    Figure Legend Snippet: A, Relative expression of interleukin‐23 receptor (IL‐23R) in lung adenocarcinoma cell line A549, lung squamous carcinoma cell line H226, and lung immortalized cell line 16HBE. Original mean value of relative expression of 16HBE was 3.84 × 10 −6 . For simplification, we set the value of 16HBE as 1, and the other values of cell lines and tissues were corrected by the baseline. B, Each pair of data points connected by a line represent the relative expression of IL‐23R from tumor and adjacent noncancerous tissues of a single donor. Black line with gray block next to each group indicates median and 25%‐75% percentile. *** P

    Techniques Used: Expressing, Blocking Assay

    2) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    3) Product Images from "Inflammatory versus Anti-Inflammatory Profiles in Major Depressive Disorders—The Role of IL-17, IL-21, IL-23, IL-35 and Foxp3"

    Article Title: Inflammatory versus Anti-Inflammatory Profiles in Major Depressive Disorders—The Role of IL-17, IL-21, IL-23, IL-35 and Foxp3

    Journal: Journal of Personalized Medicine

    doi: 10.3390/jpm11020066

    Expression of genes IL-17, IL-21, IL-23, IL-35 at the mRNA level in the study and control group (IL-17—interleukin 17, IL-21—interleukin 21, IL-23—interleukin 23, IL-35—interleukin 35, K- control group). The figure shows the arithmetic mean, minimum and maximum values of gene expression with confidence intervals of 95%.
    Figure Legend Snippet: Expression of genes IL-17, IL-21, IL-23, IL-35 at the mRNA level in the study and control group (IL-17—interleukin 17, IL-21—interleukin 21, IL-23—interleukin 23, IL-35—interleukin 35, K- control group). The figure shows the arithmetic mean, minimum and maximum values of gene expression with confidence intervals of 95%.

    Techniques Used: Expressing

    Effects of selected cytokines on Th17 cells—schematic diagram (IL-17—interleukin 17, IL-21—interleukin 21, IL-23—interleukin 23, IL-35—Interleukin 35, ‘+’—promoting effect, ‘-’—inhibitory effect, Th17—Th17 lymphocytes).
    Figure Legend Snippet: Effects of selected cytokines on Th17 cells—schematic diagram (IL-17—interleukin 17, IL-21—interleukin 21, IL-23—interleukin 23, IL-35—Interleukin 35, ‘+’—promoting effect, ‘-’—inhibitory effect, Th17—Th17 lymphocytes).

    Techniques Used:

    Expression of genes IL-17, IL-21, IL-23, IL-35 at protein level in the study and control group (IL-17—Interleukin 17, IL-21—interleukin 21, IL-23—interleukin 23, IL-35—interleukin 35, K—control group). The figure shows the arithmetic mean, minimum and maximum values of gene expression with confidence intervals of 95%.
    Figure Legend Snippet: Expression of genes IL-17, IL-21, IL-23, IL-35 at protein level in the study and control group (IL-17—Interleukin 17, IL-21—interleukin 21, IL-23—interleukin 23, IL-35—interleukin 35, K—control group). The figure shows the arithmetic mean, minimum and maximum values of gene expression with confidence intervals of 95%.

    Techniques Used: Expressing

    4) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    5) Product Images from "Human TYK2 deficiency: Mycobacterial and viral infections without hyper-IgE syndrome"

    Article Title: Human TYK2 deficiency: Mycobacterial and viral infections without hyper-IgE syndrome

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20140280

    TYK2 deficiency impairs the response to IL-23 but not to IL-27 nor IFN-γ. (A) Western blot analysis of STAT1 (pSTAT1, top) and STAT3 (pSTAT3, bottom) phosphorylation in EBV–B cells from healthy controls (C1 and C2), TYK2-deficient patients (P1 and P2), a patient with complete STAT1 deficiency (STAT1*), and an AD-HIES patient with a heterozygous STAT3 mutation (WT/T708N; STAT3*), after stimulation with 100 ng/ml IL-27 for 20 min. STAT1, STAT3, and α-tubulin levels were also assessed. The results shown are representative of at least two independent experiments. (B) Microarray analysis of HVS–T cell lines from three healthy controls, P2, and an IL-12Rβ1–deficient patient. Cells were stimulated for 12 h with 100 ng/ml IL-23. The difference between nonstimulated and stimulated cultures is shown as a fold change. (C) Western blot depicting phospho-STAT3 (pSTAT3) in EBV–B cells from a healthy control (C, C1, and C2), TYK2-deficient patients (P1, P2, P3, P5, P7, and P8), an AD-HIES patient carrying a heterozygous STAT3 mutation (WT/T708N; STAT3*), an IL-12Rβ1–deficient patient (IL-12Rβ1*), and a STAT1-deficient patient (STAT1*), without (−) and with (+) stimulation for 30 min with 100 ng/ml IL-23. α-Tubulin was used as a protein loading control. The results shown are representative of at least three independent experiments. After analysis by densitometry, a p-value
    Figure Legend Snippet: TYK2 deficiency impairs the response to IL-23 but not to IL-27 nor IFN-γ. (A) Western blot analysis of STAT1 (pSTAT1, top) and STAT3 (pSTAT3, bottom) phosphorylation in EBV–B cells from healthy controls (C1 and C2), TYK2-deficient patients (P1 and P2), a patient with complete STAT1 deficiency (STAT1*), and an AD-HIES patient with a heterozygous STAT3 mutation (WT/T708N; STAT3*), after stimulation with 100 ng/ml IL-27 for 20 min. STAT1, STAT3, and α-tubulin levels were also assessed. The results shown are representative of at least two independent experiments. (B) Microarray analysis of HVS–T cell lines from three healthy controls, P2, and an IL-12Rβ1–deficient patient. Cells were stimulated for 12 h with 100 ng/ml IL-23. The difference between nonstimulated and stimulated cultures is shown as a fold change. (C) Western blot depicting phospho-STAT3 (pSTAT3) in EBV–B cells from a healthy control (C, C1, and C2), TYK2-deficient patients (P1, P2, P3, P5, P7, and P8), an AD-HIES patient carrying a heterozygous STAT3 mutation (WT/T708N; STAT3*), an IL-12Rβ1–deficient patient (IL-12Rβ1*), and a STAT1-deficient patient (STAT1*), without (−) and with (+) stimulation for 30 min with 100 ng/ml IL-23. α-Tubulin was used as a protein loading control. The results shown are representative of at least three independent experiments. After analysis by densitometry, a p-value

    Techniques Used: Western Blot, Mutagenesis, Microarray

    6) Product Images from "In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease"

    Article Title: In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.01272

    The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant following stimulation with IL-6/IL-6R (50 ng/ml) with or without different inhibitors; ** p
    Figure Legend Snippet: The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant following stimulation with IL-6/IL-6R (50 ng/ml) with or without different inhibitors; ** p

    Techniques Used:

    Exogenous IL-6/IL-6R promotes the production of Tfh cell and B cell differentiation factors. The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant after stimulation of IL-6/IL-6R at 24 h and 48 h ( n = 6). * p
    Figure Legend Snippet: Exogenous IL-6/IL-6R promotes the production of Tfh cell and B cell differentiation factors. The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant after stimulation of IL-6/IL-6R at 24 h and 48 h ( n = 6). * p

    Techniques Used: Cell Differentiation

    Fibroblasts produce Tfh cell and B cell differentiation factors in IgG4-related RPF patients. Representative double-labeled immunofluorescence images of a-SMA and BAFF (A) ; α-SMA and IL-7 (B) ; α-SMA and IL-12 p70 (C) ; α-SMA and IL-23 (D) in the retroperitoneum tissues of IgG4-related RPF and the control retroperitoneum tissues is shown ( n = 6).
    Figure Legend Snippet: Fibroblasts produce Tfh cell and B cell differentiation factors in IgG4-related RPF patients. Representative double-labeled immunofluorescence images of a-SMA and BAFF (A) ; α-SMA and IL-7 (B) ; α-SMA and IL-12 p70 (C) ; α-SMA and IL-23 (D) in the retroperitoneum tissues of IgG4-related RPF and the control retroperitoneum tissues is shown ( n = 6).

    Techniques Used: Cell Differentiation, Labeling, Immunofluorescence

    7) Product Images from "Characterization of IL-17+ Interphotoreceptor Retinoid-Binding Protein-Specific T Cells in Experimental Autoimmune Uveitis"

    Article Title: Characterization of IL-17+ Interphotoreceptor Retinoid-Binding Protein-Specific T Cells in Experimental Autoimmune Uveitis

    Journal:

    doi: 10.1167/iovs.07-0251

    Determination of the uveitogenic activity of IRBP-specific T cells expanded by IL-23. Unfractionated T cells from IRBP1–20–immunized B6 mice at p.i. day 13 were exposed to immunizing peptide in the presence of IL-2 or IL-23 (10 ng/ mL)-containing
    Figure Legend Snippet: Determination of the uveitogenic activity of IRBP-specific T cells expanded by IL-23. Unfractionated T cells from IRBP1–20–immunized B6 mice at p.i. day 13 were exposed to immunizing peptide in the presence of IL-2 or IL-23 (10 ng/ mL)-containing

    Techniques Used: Activity Assay, Mouse Assay

    IL-23 promotes the expansion of antigen-specific and antigen-nonspecific IL-17 + T cells, whereas TGF-β and IL-6 act primarily on antigen-nonspecific IL-17 + T cells. ( A ) CD4 or CD8 T cells prepared from IRBP1–20–immunized mice (1
    Figure Legend Snippet: IL-23 promotes the expansion of antigen-specific and antigen-nonspecific IL-17 + T cells, whereas TGF-β and IL-6 act primarily on antigen-nonspecific IL-17 + T cells. ( A ) CD4 or CD8 T cells prepared from IRBP1–20–immunized mice (1

    Techniques Used: Mouse Assay

    8) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    9) Product Images from "Possible Roles of Proinflammatory Signaling in Keratinocytes Through Aryl Hydrocarbon Receptor Ligands for the Development of Squamous Cell Carcinoma"

    Article Title: Possible Roles of Proinflammatory Signaling in Keratinocytes Through Aryl Hydrocarbon Receptor Ligands for the Development of Squamous Cell Carcinoma

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.534323

    Expression of CYP1A1, cytokine, and chemokine mRNA in normal human keratinocytes (NHKCs) stimulated with FICZ, or DMBA. NHKCs were cultured and treated with FICZ (10 nM), or DMBA (1 μM) as described in the Materials and Methods. At 4 h after stimulation, total RNA was recovered from NHKCs and amplified, labeled, and analyzed. Quantitative real-time PCR was conducted to determine the number of cDNA copies for each factor, and the mRNA expression level relative to that of untreated cells was calculated for each gene and time point after normalization against glyceraldehyde 3-phosphate dehydrogenase using the ΔΔCt method (A) . NHKC culture supernatant was harvested as described in the Materials and Methods and analyzed by ELISA (B) . Data from each donor were obtained from triplicate assays, and the mean ± SD was calculated. The means of at least three independent experiments are shown. IL-23 and IL-36β production was analyzed by western blotting as described in the Materials and Methods (C) . * p
    Figure Legend Snippet: Expression of CYP1A1, cytokine, and chemokine mRNA in normal human keratinocytes (NHKCs) stimulated with FICZ, or DMBA. NHKCs were cultured and treated with FICZ (10 nM), or DMBA (1 μM) as described in the Materials and Methods. At 4 h after stimulation, total RNA was recovered from NHKCs and amplified, labeled, and analyzed. Quantitative real-time PCR was conducted to determine the number of cDNA copies for each factor, and the mRNA expression level relative to that of untreated cells was calculated for each gene and time point after normalization against glyceraldehyde 3-phosphate dehydrogenase using the ΔΔCt method (A) . NHKC culture supernatant was harvested as described in the Materials and Methods and analyzed by ELISA (B) . Data from each donor were obtained from triplicate assays, and the mean ± SD was calculated. The means of at least three independent experiments are shown. IL-23 and IL-36β production was analyzed by western blotting as described in the Materials and Methods (C) . * p

    Techniques Used: Expressing, Cell Culture, Amplification, Labeling, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Western Blot

    Immunohistochemical analysis of CCL20, IL-23, IL-17, IL-36γ, and IL-17R expression in lesion-affected skin of cSCC. Sections of cSCC lesions were deparaffinized and stained using anti-CCL20 (a–c) , anti–IL-23 (d–f) , anti–IL-17 (g,h) , anti–IL-36γ (i,j) , or anti–IL-17R (k,l) antibodies. Sections were developed with liquid permanent red. Scale bars, 100 μm. Representative specimens from 12 cases of cSCC are shown. Scale bars, 50 μm (b,i) , 100 μm (c,e–l) , and 200 μm (a,d) .
    Figure Legend Snippet: Immunohistochemical analysis of CCL20, IL-23, IL-17, IL-36γ, and IL-17R expression in lesion-affected skin of cSCC. Sections of cSCC lesions were deparaffinized and stained using anti-CCL20 (a–c) , anti–IL-23 (d–f) , anti–IL-17 (g,h) , anti–IL-36γ (i,j) , or anti–IL-17R (k,l) antibodies. Sections were developed with liquid permanent red. Scale bars, 100 μm. Representative specimens from 12 cases of cSCC are shown. Scale bars, 50 μm (b,i) , 100 μm (c,e–l) , and 200 μm (a,d) .

    Techniques Used: Immunohistochemistry, Expressing, Staining

    10) Product Images from "In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease"

    Article Title: In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.01272

    The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant following stimulation with IL-6/IL-6R (50 ng/ml) with or without different inhibitors; ** p
    Figure Legend Snippet: The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant following stimulation with IL-6/IL-6R (50 ng/ml) with or without different inhibitors; ** p

    Techniques Used:

    Exogenous IL-6/IL-6R promotes the production of Tfh cell and B cell differentiation factors. The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant after stimulation of IL-6/IL-6R at 24 h and 48 h ( n = 6). * p
    Figure Legend Snippet: Exogenous IL-6/IL-6R promotes the production of Tfh cell and B cell differentiation factors. The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant after stimulation of IL-6/IL-6R at 24 h and 48 h ( n = 6). * p

    Techniques Used: Cell Differentiation

    Fibroblasts produce Tfh cell and B cell differentiation factors in IgG4-related RPF patients. Representative double-labeled immunofluorescence images of a-SMA and BAFF (A) ; α-SMA and IL-7 (B) ; α-SMA and IL-12 p70 (C) ; α-SMA and IL-23 (D) in the retroperitoneum tissues of IgG4-related RPF and the control retroperitoneum tissues is shown ( n = 6).
    Figure Legend Snippet: Fibroblasts produce Tfh cell and B cell differentiation factors in IgG4-related RPF patients. Representative double-labeled immunofluorescence images of a-SMA and BAFF (A) ; α-SMA and IL-7 (B) ; α-SMA and IL-12 p70 (C) ; α-SMA and IL-23 (D) in the retroperitoneum tissues of IgG4-related RPF and the control retroperitoneum tissues is shown ( n = 6).

    Techniques Used: Cell Differentiation, Labeling, Immunofluorescence

    11) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    12) Product Images from "VIP-producing enteric neurons interact with innate lymphoid cells to regulate feeding-dependent intestinal epithelial barrier functions"

    Article Title: VIP-producing enteric neurons interact with innate lymphoid cells to regulate feeding-dependent intestinal epithelial barrier functions

    Journal: bioRxiv

    doi: 10.1101/721464

    VIPergic signaling promotes reduction of mucosal barrier function by VIPR2-dependent inhibition of CCR6 + ILC3. a, b , Representative FACS plot ( a ) and summaries ( b ) indicating IL-22 expression in purified CCR6 + ILC3 after in vitro IL-23 stimulation with/without VIPR2 agonist ligands. BAY: BAY-559837, VIP: vasoactive intestinal peptide. * P
    Figure Legend Snippet: VIPergic signaling promotes reduction of mucosal barrier function by VIPR2-dependent inhibition of CCR6 + ILC3. a, b , Representative FACS plot ( a ) and summaries ( b ) indicating IL-22 expression in purified CCR6 + ILC3 after in vitro IL-23 stimulation with/without VIPR2 agonist ligands. BAY: BAY-559837, VIP: vasoactive intestinal peptide. * P

    Techniques Used: Inhibition, FACS, Expressing, Purification, In Vitro

    13) Product Images from "Exacerbated Imiquimod-Induced Psoriasis-Like Skin Inflammation in IRF5-Deficient Mice"

    Article Title: Exacerbated Imiquimod-Induced Psoriasis-Like Skin Inflammation in IRF5-Deficient Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21103681

    IRF4 expression levels were upregulated in dendritic cells from the IRF5-deficient mice. ( A ) Phosphorylated NF-κB p65 expression in DCs stimulated with PBS or 1 μg/mL R848 for a quarter of an hour or one hour was measured. ( B ) IRF4 mRNA expression levels in DCs before and after stimulation with 1 μg/mL R848 for 24 h were determined by quantitative RT-PCR. Data are obtained from duplicate samples from 12 mice in each group. Values are presented as the mean ± SEM of three independent experiments. ( C , E ) Representative flowcytometryplots of IRF4 + IL-23 + DCs before ( C ) and after ( E ) stimulation with 1 μg/mL R848 for 3 h. ( D , F ) The frequency of IRF4 + IL-23 + DCs before ( D ) and after ( F ) stimulation with 1 μg/mL R848 for 3 h. Data are obtained from 5 mice in each group. Values are presented as mean the ± SEM of two independent experiments.
    Figure Legend Snippet: IRF4 expression levels were upregulated in dendritic cells from the IRF5-deficient mice. ( A ) Phosphorylated NF-κB p65 expression in DCs stimulated with PBS or 1 μg/mL R848 for a quarter of an hour or one hour was measured. ( B ) IRF4 mRNA expression levels in DCs before and after stimulation with 1 μg/mL R848 for 24 h were determined by quantitative RT-PCR. Data are obtained from duplicate samples from 12 mice in each group. Values are presented as the mean ± SEM of three independent experiments. ( C , E ) Representative flowcytometryplots of IRF4 + IL-23 + DCs before ( C ) and after ( E ) stimulation with 1 μg/mL R848 for 3 h. ( D , F ) The frequency of IRF4 + IL-23 + DCs before ( D ) and after ( F ) stimulation with 1 μg/mL R848 for 3 h. Data are obtained from 5 mice in each group. Values are presented as mean the ± SEM of two independent experiments.

    Techniques Used: Expressing, Mouse Assay, Quantitative RT-PCR

    IRF5 deficiency downregulates IL-10 expression and upregulates IL-23 expression by dendritic cells. Dendritic cells (DCs) from the wild-type (WT) or IRF5 knockout (IRF5 KO) mice were stimulated with phosphate-buffered saline (PBS) or 1 µg/mL R848 for 24 h. Messenger RNA levels of IL-10 ( A ), IL-23p19 ( B ) and IL-12/23p40 ( C ) were determined by quantitative RT-PCR. Protein levels of IL-10 in the supernatants after R848 stimulation ( D ) IL-23 in the supernatants without R848 stimulation ( E ) were determined by enzyme-linked immunosorbent assay (ELISA). Data are obtained from duplicate samples from 12 mice in each group. Values are presented as mean the ± SEM of three independent experiments.
    Figure Legend Snippet: IRF5 deficiency downregulates IL-10 expression and upregulates IL-23 expression by dendritic cells. Dendritic cells (DCs) from the wild-type (WT) or IRF5 knockout (IRF5 KO) mice were stimulated with phosphate-buffered saline (PBS) or 1 µg/mL R848 for 24 h. Messenger RNA levels of IL-10 ( A ), IL-23p19 ( B ) and IL-12/23p40 ( C ) were determined by quantitative RT-PCR. Protein levels of IL-10 in the supernatants after R848 stimulation ( D ) IL-23 in the supernatants without R848 stimulation ( E ) were determined by enzyme-linked immunosorbent assay (ELISA). Data are obtained from duplicate samples from 12 mice in each group. Values are presented as mean the ± SEM of three independent experiments.

    Techniques Used: Expressing, Knock-Out, Mouse Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    14) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    15) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    16) Product Images from "Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma, et al. Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma"

    Article Title: Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma, et al. Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma

    Journal: Cancer Science

    doi: 10.1111/cas.14343

    Rank sum test analysis of the relationship between the balance of 2 cytokines, soluble interleukin‐23 receptor (sIL‐23R) and IL‐23, and N stage (A), T stage (B) and IL‐17 (C) of non‐small cell lung cancer patients. The ordinate was the rank mean. * P
    Figure Legend Snippet: Rank sum test analysis of the relationship between the balance of 2 cytokines, soluble interleukin‐23 receptor (sIL‐23R) and IL‐23, and N stage (A), T stage (B) and IL‐17 (C) of non‐small cell lung cancer patients. The ordinate was the rank mean. * P

    Techniques Used:

    A, Kaplan‐Meier analysis of serum soluble interleukin‐23 receptor (sIL‐23R) for non‐small cell lung cancer (NSCLC) patient prognosis. B, Merged survival curves of NSCLC patient subgroups with high ( > 2.56), intermediate (0.43‐2.56), and low (
    Figure Legend Snippet: A, Kaplan‐Meier analysis of serum soluble interleukin‐23 receptor (sIL‐23R) for non‐small cell lung cancer (NSCLC) patient prognosis. B, Merged survival curves of NSCLC patient subgroups with high ( > 2.56), intermediate (0.43‐2.56), and low (

    Techniques Used:

    Identification of soluble interleukin‐23 receptor (sIL‐23R) in non‐small cell lung cancer (NSCLC) and healthy control serum samples. A, Gel was visualized by silver staining. The mass spectroscopy (MS) results were analyzed after removing chemical and electrical signal noises, and through a series of processing such as data correction and normalization. Left panels, normal control; right panels, NSCLC. Red arrow in the box indicates the discrepant masses corresponding to sIL‐23R. B, The protein spot with discrepancies between healthy controls and NSCLC patients was analyzed using MALDI time of flight (TOF)/TOF MS. C, sIL‐23R fragments showed 12.2% amino acid sequence coverage in the MS analysis
    Figure Legend Snippet: Identification of soluble interleukin‐23 receptor (sIL‐23R) in non‐small cell lung cancer (NSCLC) and healthy control serum samples. A, Gel was visualized by silver staining. The mass spectroscopy (MS) results were analyzed after removing chemical and electrical signal noises, and through a series of processing such as data correction and normalization. Left panels, normal control; right panels, NSCLC. Red arrow in the box indicates the discrepant masses corresponding to sIL‐23R. B, The protein spot with discrepancies between healthy controls and NSCLC patients was analyzed using MALDI time of flight (TOF)/TOF MS. C, sIL‐23R fragments showed 12.2% amino acid sequence coverage in the MS analysis

    Techniques Used: Silver Staining, Mass Spectrometry, Sequencing

    A, Relative expression of interleukin‐23 receptor (IL‐23R) in lung adenocarcinoma cell line A549, lung squamous carcinoma cell line H226, and lung immortalized cell line 16HBE. Original mean value of relative expression of 16HBE was 3.84 × 10 −6 . For simplification, we set the value of 16HBE as 1, and the other values of cell lines and tissues were corrected by the baseline. B, Each pair of data points connected by a line represent the relative expression of IL‐23R from tumor and adjacent noncancerous tissues of a single donor. Black line with gray block next to each group indicates median and 25%‐75% percentile. *** P
    Figure Legend Snippet: A, Relative expression of interleukin‐23 receptor (IL‐23R) in lung adenocarcinoma cell line A549, lung squamous carcinoma cell line H226, and lung immortalized cell line 16HBE. Original mean value of relative expression of 16HBE was 3.84 × 10 −6 . For simplification, we set the value of 16HBE as 1, and the other values of cell lines and tissues were corrected by the baseline. B, Each pair of data points connected by a line represent the relative expression of IL‐23R from tumor and adjacent noncancerous tissues of a single donor. Black line with gray block next to each group indicates median and 25%‐75% percentile. *** P

    Techniques Used: Expressing, Blocking Assay

    17) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    18) Product Images from "Genetic and pharmacological inhibition of the nuclear receptor RORα regulates TH17 driven inflammatory disorders"

    Article Title: Genetic and pharmacological inhibition of the nuclear receptor RORα regulates TH17 driven inflammatory disorders

    Journal: Nature Communications

    doi: 10.1038/s41467-020-20385-9

    SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + IL-23+IL-1β) and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.
    Figure Legend Snippet: SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + IL-23+IL-1β) and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.

    Techniques Used: Expressing, Flow Cytometry, FACS, Two Tailed Test

    19) Product Images from "IL-23 promotes maintenance but not commitment to the Th17 lineage 1"

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    Journal:

    doi:

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Figure Legend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Techniques Used: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five
    Figure Legend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Techniques Used: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional
    Figure Legend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Techniques Used: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β
    Figure Legend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Techniques Used: Cell Culture

    20) Product Images from "'Psoriasis 1' reduces T-lymphocyte-mediated inflammation in patients with psoriasis by inhibiting vitamin D receptor-mediated STAT4 inactivation"

    Article Title: 'Psoriasis 1' reduces T-lymphocyte-mediated inflammation in patients with psoriasis by inhibiting vitamin D receptor-mediated STAT4 inactivation

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4695

    'Psoriasis 1' inhibited inflammatory response following VDR-siRNA transfection. T lymphocytes were isolated from patients with psoriasis and transfected with NC or VDR siRNA. Effects of high-dose 'Psoriasis 1' and triptolide on the levels of (A) TNF-α, (B) IFN-γ, (C) IL-2, (D) IL-6, (E) TGF-β, (F) IL-4, (G) IL-23, (H) IL-17 and (I) VD in T lymphocytes after VDR-siRNA transfection. Data are presented as mean ± standard deviation. ** P
    Figure Legend Snippet: 'Psoriasis 1' inhibited inflammatory response following VDR-siRNA transfection. T lymphocytes were isolated from patients with psoriasis and transfected with NC or VDR siRNA. Effects of high-dose 'Psoriasis 1' and triptolide on the levels of (A) TNF-α, (B) IFN-γ, (C) IL-2, (D) IL-6, (E) TGF-β, (F) IL-4, (G) IL-23, (H) IL-17 and (I) VD in T lymphocytes after VDR-siRNA transfection. Data are presented as mean ± standard deviation. ** P

    Techniques Used: Transfection, Isolation, Standard Deviation

    'Psoriasis 1' inhibits inflammatory response in T lymphocytes. Effects of 'Psoriasis 1' at different doses and triptolide on the levels of (A) TNF-α, (B) IFN-γ, (C) IL-2, (D) IL-6, (E) TGF-β, (F) IL-4, (G) IL-23, (H) IL-17 and (I) VD in T lymphocytes. Data are presented as mean ± standard deviation. * P
    Figure Legend Snippet: 'Psoriasis 1' inhibits inflammatory response in T lymphocytes. Effects of 'Psoriasis 1' at different doses and triptolide on the levels of (A) TNF-α, (B) IFN-γ, (C) IL-2, (D) IL-6, (E) TGF-β, (F) IL-4, (G) IL-23, (H) IL-17 and (I) VD in T lymphocytes. Data are presented as mean ± standard deviation. * P

    Techniques Used: Standard Deviation

    Related Articles

    Recombinant:

    Article Title: IRAK4 kinase Activity is Required for Th17 Differentiation and Th17-mediated Disease
    Article Snippet: Naïve CD4+ CD62L+ T cells were purified by AutoMacs according to the manufacturer’s protocol (Miltenyi Biotech, Auburn, CA). .. Recombinant cytokines including IL-6, TGF-β, IL-23, and IL-1β were purchased from R & D Systems, Inc. (Minneapolis, MN). .. Recombinant IL-1RA was commercially prescribed as Kineret® (Amgen Inc.

    Cell Culture:

    Article Title: IL-23 induces human osteoclastogenesis via IL-17 in vitro, and anti-IL-23 antibody attenuates collagen-induced arthritis in rats
    Article Snippet: Plates were coated overnight with anti-CD3 mAb (0.1 μg/ml; Beckman Coulter, Fullerton, CA, USA) and then washed; anti-CD28 mAb (2 μg/ml; Beckman Coulter) was then added to each well. .. At the beginning of cell culture, rhIL-23 was added at 1, 3, or 10 ng/ml. ..

    Article Title: Interleukin-12 (IL-12) and IL-23 Induction of Substance P Synthesis in Murine T Cells and Macrophages Is Subject to IL-10 and Transforming Growth Factor β Regulation
    Article Snippet: The LPMC from the terminal ilea of WT mice were isolated by using paramagnetic beads coupled to mouse anti-F4/80 monoclonal antibody to capture macrophages. .. The isolated cells were cultured for 4 h in vitro with or without rIL-23. ..

    SDS Page:

    Article Title: Autoantibodies against IL-17A, IL-17F, and IL-22 in patients with chronic mucocutaneous candidiasis and autoimmune polyendocrine syndrome type I
    Article Snippet: .. We subjected 500 ng rIL-17A, rIL-17, rIL-22, rIL-23 (R & D Systems), or BSA to SDS-PAGE (10% acrylamide) under reducing conditions. .. The protein bands were electroblotted onto nitrocellulose membranes (iBlot Gel Transfer Stacks; Invitrogen).

    other:

    Article Title: IL-23 induces human osteoclastogenesis via IL-17 in vitro, and anti-IL-23 antibody attenuates collagen-induced arthritis in rats
    Article Snippet: As shown in Figure , rhIL-23 increased the number of CD51-positive osteoclasts in a dose-dependent manner even in the absence of osteoblasts or exogenous sRANKL.

    Article Title: IL-23 induces human osteoclastogenesis via IL-17 in vitro, and anti-IL-23 antibody attenuates collagen-induced arthritis in rats
    Article Snippet: The effect of osteoclastogenesis induced by IL-23 was maximal at 1.0 ng/ml rhIL-23 (Figure ).

    Isolation:

    Article Title: Interleukin-12 (IL-12) and IL-23 Induction of Substance P Synthesis in Murine T Cells and Macrophages Is Subject to IL-10 and Transforming Growth Factor β Regulation
    Article Snippet: The LPMC from the terminal ilea of WT mice were isolated by using paramagnetic beads coupled to mouse anti-F4/80 monoclonal antibody to capture macrophages. .. The isolated cells were cultured for 4 h in vitro with or without rIL-23. ..

    In Vitro:

    Article Title: Interleukin-12 (IL-12) and IL-23 Induction of Substance P Synthesis in Murine T Cells and Macrophages Is Subject to IL-10 and Transforming Growth Factor β Regulation
    Article Snippet: The LPMC from the terminal ilea of WT mice were isolated by using paramagnetic beads coupled to mouse anti-F4/80 monoclonal antibody to capture macrophages. .. The isolated cells were cultured for 4 h in vitro with or without rIL-23. ..

    Expressing:

    Article Title: Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage
    Article Snippet: Typically 100 μl of the induced cultures are added to each well of a deep-well block (Thomson) for staining of membrane associated proteins and specific antigens. .. Display of wild-type and alanine mutants of IL-23 on yeastIL-23 protein expression on the yeast surface was assessed by flow cytometry by antibodies specific for the p40 subunit (R & D Systems) and V5 tag (Invitrogen) using Fortessa X-20. ..

    Flow Cytometry:

    Article Title: Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage
    Article Snippet: Typically 100 μl of the induced cultures are added to each well of a deep-well block (Thomson) for staining of membrane associated proteins and specific antigens. .. Display of wild-type and alanine mutants of IL-23 on yeastIL-23 protein expression on the yeast surface was assessed by flow cytometry by antibodies specific for the p40 subunit (R & D Systems) and V5 tag (Invitrogen) using Fortessa X-20. ..

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    R&D Systems interleukin 3
    LCN2 levels are elevated in PMF MNC-CM, and LCN2 is localized to MF marrow myeloid cells. (A) LCN2 levels in media conditioned with MF or PV MNCs (n = 10 and n = 7, respectively) and normal bone marrow MNCs (n = 10) after incubation in serum-free expansion medium containing stem cell factor, thrombopoietin, fms-like tyrosine kinase 3 ligand, and <t>interleukin-3</t> for 1 day (Kruskal-Wallis nonparametric ANOVA; P
    Interleukin 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IL-1β increases <t>IL-23</t> stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of
    Il 23, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
    il 23 - by Bioz Stars, 2021-05
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    94
    R&D Systems recombinant cytokines
    Regulation of other <t>cytokines</t> by ATM kinase
    Recombinant Cytokines, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    recombinant cytokines - by Bioz Stars, 2021-05
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    93
    R&D Systems ril 23
    IL-23 stimulates macrophages and IL-12 stimulates T cells to produce SP. Splenic T cells or macrophages isolated as described in the text were incubated for 6 h with or without rIL-12 or <t>rIL-23</t> at 0.5 ng/ml. SP was extracted from the cells after the 6 h of incubation and quantified by an ELISA. The data are means of three determinations from three separate experiments ± the standard deviation (SD). For cells versus cells plus IL-12 or cells plus IL-23, P
    Ril 23, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ril 23 - by Bioz Stars, 2021-05
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    Image Search Results


    LCN2 levels are elevated in PMF MNC-CM, and LCN2 is localized to MF marrow myeloid cells. (A) LCN2 levels in media conditioned with MF or PV MNCs (n = 10 and n = 7, respectively) and normal bone marrow MNCs (n = 10) after incubation in serum-free expansion medium containing stem cell factor, thrombopoietin, fms-like tyrosine kinase 3 ligand, and interleukin-3 for 1 day (Kruskal-Wallis nonparametric ANOVA; P

    Journal: Blood

    Article Title: Lipocalin produced by myelofibrosis cells affects the fate of both hematopoietic and marrow microenvironmental cells

    doi: 10.1182/blood-2014-12-618595

    Figure Lengend Snippet: LCN2 levels are elevated in PMF MNC-CM, and LCN2 is localized to MF marrow myeloid cells. (A) LCN2 levels in media conditioned with MF or PV MNCs (n = 10 and n = 7, respectively) and normal bone marrow MNCs (n = 10) after incubation in serum-free expansion medium containing stem cell factor, thrombopoietin, fms-like tyrosine kinase 3 ligand, and interleukin-3 for 1 day (Kruskal-Wallis nonparametric ANOVA; P

    Article Snippet: CD34+ cells were plated in 30-mm dishes containing 1 mL of serum-free expansion medium with 1.1% methylcellulose, to which stem cell factor, thrombopoietin, fms-like tyrosine kinase 3 ligand, granulocyte macrophage–colony stimulating factor, interleukin-3, and erythropoietin were added with or without LCN2.

    Techniques: Peptide Mass Fingerprinting, Incubation

    IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Journal:

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    doi:

    Figure Lengend Snippet: IL-1β increases IL-23 stimulated maintenance of the Th17 phenotype. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation in the presence of

    Article Snippet: Since IL-23 was capable of maintaining the IL-17-secreting phenotype, it allowed us to determine if IL-23 mediated commitment to the Th17 lineage, commitment being defined as the ability of cells to maintain the IL-17-secreting phenotype in the presence of cytokines promoting the development of other subsets.

    Techniques: Cell Culture

    IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Journal:

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    doi:

    Figure Lengend Snippet: IL-23 maintains the IL-17 secreting phenotype without affecting cell expansion or survival. A, Naïve CD4+ T cells were activated, cultured in TGF-β+IL-6+IL-1β and blocking antibodies (anti-IFN-γ and anti-IL-4) for five

    Article Snippet: Since IL-23 was capable of maintaining the IL-17-secreting phenotype, it allowed us to determine if IL-23 mediated commitment to the Th17 lineage, commitment being defined as the ability of cells to maintain the IL-17-secreting phenotype in the presence of cytokines promoting the development of other subsets.

    Techniques: Cell Culture, Blocking Assay

    IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Journal:

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    doi:

    Figure Lengend Snippet: IL-23 does not program commitment to the Th17 lineage. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round followed by 2 rounds of stimulation in IL-23 + IL-1β, were cultured for an additional

    Article Snippet: Since IL-23 was capable of maintaining the IL-17-secreting phenotype, it allowed us to determine if IL-23 mediated commitment to the Th17 lineage, commitment being defined as the ability of cells to maintain the IL-17-secreting phenotype in the presence of cytokines promoting the development of other subsets.

    Techniques: Cell Culture

    IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Journal:

    Article Title: IL-23 promotes maintenance but not commitment to the Th17 lineage 1

    doi:

    Figure Lengend Snippet: IL-1b increases responsiveness of the Il17 locus. A, Naïve T cells were activated, primed with TGF-β+IL-6+IL-1β for the first round and cultured for 2 additional rounds of stimulation with IL-23, IL-1β, or IL-23+IL-1β

    Article Snippet: Since IL-23 was capable of maintaining the IL-17-secreting phenotype, it allowed us to determine if IL-23 mediated commitment to the Th17 lineage, commitment being defined as the ability of cells to maintain the IL-17-secreting phenotype in the presence of cytokines promoting the development of other subsets.

    Techniques: Cell Culture

    Regulation of other cytokines by ATM kinase

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: The Ataxia Telangiectasia Mutated Kinase pathway Regulates IL-23 Expression by Human Dendritic Cells

    doi: 10.4049/jimmunol.1201484

    Figure Lengend Snippet: Regulation of other cytokines by ATM kinase

    Article Snippet: Cells were differentiated in the presence of combinations of recombinant cytokines (IL-1β [5ng/ml], IL-6 [5ng/ml], IL-23 [10ng/ml], TGF-β [5ng/ml], all R & D Systems) and co-stimulated with anti-CD28 (5μg/ml) and anti-CD3 (OKT3, 1μg/ml) antibodies in the presence of low-dose IL-2 (50IU/ml, R & D Systems) ( ).

    Techniques:

    IL-23 stimulates macrophages and IL-12 stimulates T cells to produce SP. Splenic T cells or macrophages isolated as described in the text were incubated for 6 h with or without rIL-12 or rIL-23 at 0.5 ng/ml. SP was extracted from the cells after the 6 h of incubation and quantified by an ELISA. The data are means of three determinations from three separate experiments ± the standard deviation (SD). For cells versus cells plus IL-12 or cells plus IL-23, P

    Journal: Infection and Immunity

    Article Title: Interleukin-12 (IL-12) and IL-23 Induction of Substance P Synthesis in Murine T Cells and Macrophages Is Subject to IL-10 and Transforming Growth Factor β Regulation

    doi: 10.1128/IAI.00358-08

    Figure Lengend Snippet: IL-23 stimulates macrophages and IL-12 stimulates T cells to produce SP. Splenic T cells or macrophages isolated as described in the text were incubated for 6 h with or without rIL-12 or rIL-23 at 0.5 ng/ml. SP was extracted from the cells after the 6 h of incubation and quantified by an ELISA. The data are means of three determinations from three separate experiments ± the standard deviation (SD). For cells versus cells plus IL-12 or cells plus IL-23, P

    Article Snippet: The isolated cells were cultured for 4 h in vitro with or without rIL-23.

    Techniques: Isolation, Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation

    IL-23 induces macrophages, while IL-12 induces T cells to express PPT A mRNA. Macrophages (F/480 + ) or T cells (Thy 1.2 + ) were positively selected from dispersed splenocytes of CBA/J schistosome-infected mice. Cells were cultured in the presence or absence of rIL-2 or rIL-23 at the indicated concentrations for 4 h. Cellular RNA was extracted, reverse transcribed, and subjected to quantitative RT-PCR to measure the PPT A transcript number after the incubation. The data are means of multiple determinations from three separate experiments ± the standard error (SE).

    Journal: Infection and Immunity

    Article Title: Interleukin-12 (IL-12) and IL-23 Induction of Substance P Synthesis in Murine T Cells and Macrophages Is Subject to IL-10 and Transforming Growth Factor β Regulation

    doi: 10.1128/IAI.00358-08

    Figure Lengend Snippet: IL-23 induces macrophages, while IL-12 induces T cells to express PPT A mRNA. Macrophages (F/480 + ) or T cells (Thy 1.2 + ) were positively selected from dispersed splenocytes of CBA/J schistosome-infected mice. Cells were cultured in the presence or absence of rIL-2 or rIL-23 at the indicated concentrations for 4 h. Cellular RNA was extracted, reverse transcribed, and subjected to quantitative RT-PCR to measure the PPT A transcript number after the incubation. The data are means of multiple determinations from three separate experiments ± the standard error (SE).

    Article Snippet: The isolated cells were cultured for 4 h in vitro with or without rIL-23.

    Techniques: Crocin Bleaching Assay, Infection, Mouse Assay, Cell Culture, Quantitative RT-PCR, Incubation

    PPT A expression in LP macrophages is subject to IL-23 and TGF-β regulation. Isolated LP macrophages from healthy mice were incubated 4 h with or without rIL-23 in the presence or absence of TGF-β all at 1 ng/ml. After the incubation, cellular RNA was extracted and analyzed for PPT A mRNA expression using PCR. The results from two individual experiments are shown. All samples contained comparable amounts of the HPRT housekeeping gene transcripts.

    Journal: Infection and Immunity

    Article Title: Interleukin-12 (IL-12) and IL-23 Induction of Substance P Synthesis in Murine T Cells and Macrophages Is Subject to IL-10 and Transforming Growth Factor β Regulation

    doi: 10.1128/IAI.00358-08

    Figure Lengend Snippet: PPT A expression in LP macrophages is subject to IL-23 and TGF-β regulation. Isolated LP macrophages from healthy mice were incubated 4 h with or without rIL-23 in the presence or absence of TGF-β all at 1 ng/ml. After the incubation, cellular RNA was extracted and analyzed for PPT A mRNA expression using PCR. The results from two individual experiments are shown. All samples contained comparable amounts of the HPRT housekeeping gene transcripts.

    Article Snippet: The isolated cells were cultured for 4 h in vitro with or without rIL-23.

    Techniques: Expressing, Isolation, Mouse Assay, Incubation, Polymerase Chain Reaction