Journal: NPJ Precision Oncology

Article Title: RICH1 enhances pro-inflammatory TAM infiltration in breast cancer via promoting TRIM21-mediated ubiquitination of RhoA and inhibiting STAT3 phosphorylation

doi: 10.1038/s41698-025-01252-6

Figure Lengend Snippet: A LogFC of mRNA expression level of chemokines, which polarize macrophages towards M1 or M2 in 4T1-RICH1 OE/NC. B The secretion level of IFN-γ, CXCL9, IL-4, and IL-13 was evaluated through ELISA between 4T1-RICH1 OE/NC. C The PCR products by RICH1 primers were analyzed by electrophoresis in agarose gels stained with ethidium bromide, which were extracted from HC11-RICH1 K/O1/2/ NC established using CRISPR/Cas9 RICH1/NC-targeted lentivirus and picking monoclonal. D The secretion level of IFN-γ, CXCL9, IL-4, and IL-13 was evaluated through ELISA between HC11-RICH1 K/O1/2/ NC. E The volume and weight of the tumors compared in the mice injected with 4T1-RICH1 OE shIFNG/4T1-RICH1 OE. F The proportions of CD86 + subpopulations in CD45 + CD11b + F4/80 + cells of TME in the above tissues by flow cytometry analysis. Abbreviation: FC fold change, ELISA enzyme linked immunosorbent assay, PCR polymerase chain reaction. P values in ( A , B , D , E and F ) were calculated using unpaired two-tailed Student’s t tests. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The concentrations of IFN-γ, CXCL9, IL-4, and IL-13 in the cell medium were measured using enzyme linked immunosorbent assay (ELISA) kits specific for mouse IFN-γ, CXCL9, IL-4, and IL-13 (Multi Sciences, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Electrophoresis, Staining, CRISPR, Injection, Flow Cytometry, Polymerase Chain Reaction, Two Tailed Test