il 1 beta human singleplex kit  (Thermo Fisher)


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    Name:
    IL 1 beta Human Singleplex Kit
    Description:
    This singleplex bead kit is specifically designed for measurement of human IL 1β in serum plasma and tissue culture supernatant This assay may be run alone or in combination with other select Luminex singleplex kits The assay is suitable for use with the Luminex 100 200 and FLEXMAP 3D systems Buffer reagents needed to complete the reaction are sold separately Cat No LHB0001 • Superior performance accurate reproducible and sensitive quantitation of multiple proteins• High quality fully qualified antibodies permit excellent specificity and sensitivity• Fast and easy protocols perform your assay and analyze your data typically in less than one dayLuminex xMAP Technology Effective and Proven Analytical ToolThe IL 1β Human Singleplex Bead Kit is based on xMAP technology The use of a suspension bead based technology enables the multiplexing capabilities of the Luminex assays Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities Each bead is given a unique number or bead region allowing differentiation of one bead from another Beads covalently bound to different antibodies can be mixed in the same assay utilizing a 96 well microplate format Upon completion of the sandwich immunoassay beads must be measured using a Luminex detection system 100 200 or FLEXMAP 3D The instrument uses xPONENT software to distinguish bead color analyte and R PE fluorescence intensity assay signal strength to quantify target s More Validation for Confidence in Your ResultsThe IL 1β Human Singleplex Bead Kit has been validated using the same rigorous criterion that distinguishes Luminex assays from other commercially available assays With more than 20 years of experience our fully qualified antibodies help ensure the specificity and sensitivity of our assays When benchmarked against the competition our multiplex products consistently deliver excellent results Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay see Documents section below Standard curves Figure 1 and 2 are generated for each lot of product to assure quantitation consistency Learn more about all Invitrogen assays for the Luminex platform
    Catalog Number:
    lhc0011
    Price:
    None
    Applications:
    Protein Assays and Analysis|Protein Biology
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher il 1 beta human singleplex kit
    This singleplex bead kit is specifically designed for measurement of human IL 1β in serum plasma and tissue culture supernatant This assay may be run alone or in combination with other select Luminex singleplex kits The assay is suitable for use with the Luminex 100 200 and FLEXMAP 3D systems Buffer reagents needed to complete the reaction are sold separately Cat No LHB0001 • Superior performance accurate reproducible and sensitive quantitation of multiple proteins• High quality fully qualified antibodies permit excellent specificity and sensitivity• Fast and easy protocols perform your assay and analyze your data typically in less than one dayLuminex xMAP Technology Effective and Proven Analytical ToolThe IL 1β Human Singleplex Bead Kit is based on xMAP technology The use of a suspension bead based technology enables the multiplexing capabilities of the Luminex assays Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities Each bead is given a unique number or bead region allowing differentiation of one bead from another Beads covalently bound to different antibodies can be mixed in the same assay utilizing a 96 well microplate format Upon completion of the sandwich immunoassay beads must be measured using a Luminex detection system 100 200 or FLEXMAP 3D The instrument uses xPONENT software to distinguish bead color analyte and R PE fluorescence intensity assay signal strength to quantify target s More Validation for Confidence in Your ResultsThe IL 1β Human Singleplex Bead Kit has been validated using the same rigorous criterion that distinguishes Luminex assays from other commercially available assays With more than 20 years of experience our fully qualified antibodies help ensure the specificity and sensitivity of our assays When benchmarked against the competition our multiplex products consistently deliver excellent results Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay see Documents section below Standard curves Figure 1 and 2 are generated for each lot of product to assure quantitation consistency Learn more about all Invitrogen assays for the Luminex platform
    https://www.bioz.com/result/il 1 beta human singleplex kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 1 beta human singleplex kit - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument. .. Morphological analysis and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay .

    Cytometry:

    Article Title: Doxycycline-mediated effects on persistent symptoms and systemic cytokine responses post-neuroborreliosis: a randomized, prospective, cross-over study
    Article Snippet: Levels of IL-6 and IL-8 in PBMC supernatants were analyzed using single-bead kits (LHC0061 and LHC0081, Invitrogen), whereas a six-plex kit was used (with an extracellular protein buffer reagent kit [LHB0001], Invitrogen) for detection of IL-1β (LHC0011), IL-4 (LHC0041), IL-10 (LHC0101), IFN-γ (LHC4031), GM-CSF (LHC2011) and TNF (LHC3011, all from Invitrogen) in the cell supernatants. .. Levels of IL-6 and IL-8 in PBMC supernatants were analyzed using single-bead kits (LHC0061 and LHC0081, Invitrogen), whereas a six-plex kit was used (with an extracellular protein buffer reagent kit [LHB0001], Invitrogen) for detection of IL-1β (LHC0011), IL-4 (LHC0041), IL-10 (LHC0101), IFN-γ (LHC4031), GM-CSF (LHC2011) and TNF (LHC3011, all from Invitrogen) in the cell supernatants.

    Microscopy:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument. .. Morphological of hippocampal neurons was analyzed by microscopy (Bx51; Olympus Corporation; Shinjuku-ku, Japan).

    Mouse Assay:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument. .. Apoptotic neuronal cells in hippocampus in experimental mice were analyzed using TUNEL assay (DeadEnd™ Colorimetric Tunel system; Promega Corporation; Madison, WI, USA) according to the manufacturer's instructions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: .. Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument. .. Morphological analysis and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay .

    Expressing:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: .. Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument. .. Morphological analysis and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay .

    End Labeling:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument. .. Morphological analysis and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay .

    Lysis:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: ELISA Hippocampal neurons (1×107 cells) were prepared and lysed using cells lysis buffer (FNN0021; Thermo Fisher Scientific, Inc.). .. Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument.

    Binding Assay:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: .. Expression levels of neprilysin (15-0106-42), insulin (PA5-29350), Abeta-42 (437900), Abeta-40 (KMB3441), interleukin (IL)-1β (LHC0011M), monocyte chemoattractant protein (MCP)-1 (88-7391-88), insulin-like growth factor binding protein (IGFBP)-3 (EMIGFBP3) and vascular endothelial growth factor (VEGF)-β (MBS3205) were analyzed using ELISA kits (all Thermo Fisher Scientific, Inc.) according to the manufacturer's instrument. .. Morphological analysis and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay .

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  • 99
    Thermo Fisher il 1 beta human singleplex kit
    Il 1 Beta Human Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 1 beta human singleplex kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 1 beta human singleplex kit - by Bioz Stars, 2020-03
    99/100 stars
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