Structured Review

Roche igepal 630 nonidet p 40
Igepal 630 Nonidet P 40, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igepal 630 nonidet p 40/product/Roche
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
igepal 630 nonidet p 40 - by Bioz Stars, 2020-04
87/100 stars

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Related Articles

Protein Binding:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ]. .. Nonspecific protein binding was blocked with 5% nonfat milk in PBS for 1 h and then incubated with primary antibodies at 1 : 1000 dilutions for 2 days.

Cell Culture:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: Western Blot Analysis 4 × 105 cells/well cultured MG-63 cells were seeded to osteogenic medium with various concentrations of DBT in a 6-well culture plate. .. After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ].

Concentration Assay:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ]. .. The concentration of protein was measured using a BCA protein assay kit (Pierce, Rockford, IL, USA).

Incubation:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ]. .. Nonspecific protein binding was blocked with 5% nonfat milk in PBS for 1 h and then incubated with primary antibodies at 1 : 1000 dilutions for 2 days.

Polyacrylamide Gel Electrophoresis:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ]. .. Equal amounts of protein were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes.

BIA-KA:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ]. .. The concentration of protein was measured using a BCA protein assay kit (Pierce, Rockford, IL, USA).

Western Blot:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: Paragraph title: 2.6. Western Blot Analysis ... After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ].

Lysis:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: .. After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ]. .. The concentration of protein was measured using a BCA protein assay kit (Pierce, Rockford, IL, USA).

SDS Page:

Article Title: Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective
Article Snippet: After culturing for 7 days, adherent cells were washed and immersed in ice-cold lysis buffer containing 50 mM Tris (pH 7.5), 1 mM EDTA (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM β -mercaptoethanol, 1% IGEPAL-630/Nonidet P-40, and proteinase inhibitor cocktail (Roche, Basel, Switzerland) [ ]. .. Equal amounts of protein were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes.

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