ige detection immunoassays  (Thermo Fisher)


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    IgE Detection Antibody
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    81-1016-01
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    Thermo Fisher ige detection immunoassays

    https://www.bioz.com/result/ige detection immunoassays/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ige detection immunoassays - by Bioz Stars, 2020-03
    99/100 stars

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    immunocap phadia 250

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    Related Articles

    Diagnostic Assay:

    Article Title: Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure
    Article Snippet: Diagnostic performance characteristics of the standard Phadebas RAST, modified RAST, and Pharmacia CAP system versus skin testing. .. Comparison of singleplex specific IgE detection immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy.

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: A number of pitfalls affect the reliability of these diagnostic approaches, due to the poor quality of the extracts, their unknown component content and sometimes the presence of unwanted contaminants . .. In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , .

    Binding Assay:

    Article Title: Antigenic determinants of Der p 1: Specificity and cross-reactivity associated with IgE antibody recognition
    Article Snippet: Paragraph title: IgE antibody direct binding assays ... IgE detection and quantification in plasma samples was determined using Specific IgE detection streptavidin-ImmunoCAP on the Phadia® 100 (Thermo Fisher Scientific, Portage, MI).

    Positive Control:

    Article Title: Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines
    Article Snippet: For OVA- and NP-specific IgG1 or IgE detection, we incubated serially diluted sera from immunized mice on 96-well Immulon plates (Thermo Scientific) coated with OVA (grade VII, Sigma-Aldrich) at 100 μg/ml or NP at 10 μg/ml. .. To determine relative units, we used positive control serum samples from C57BL/6 mice that contained OVA-specific or NP-specific Abs.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Allergen valency, dose and FcεRI occupancy set thresholds for secretory responses to Pen a 1 and motivate design of hypoallergens
    Article Snippet: Paragraph title: ELISA assay for reactivity of IgE in human plasma to Pen a 1 ... For IgE detection, wells were thoroughly washed and incubated with polyclonal goat anti-human IgE-HRP (Invitrogen, Grand Island, NY).

    Article Title: In Utero Exposure to Environmental Tobacco Smoke Potentiates Adult Responses to Allergen in BALB/c Mice
    Article Snippet: For OVA-specific IgE detection, 96-well Immulon4 plates (Thermo Electron Corp., Waltham, MA) coated with monoclonal rat anti-mouse IgE (5 μg/mL in PBS, 100 μL/well; BD-Pharmingen) captured serum IgE. .. We used a standard ELISA reagent kit (OptEIA Reagent Set B; BD-Pharmingen) and converted OD450 readings of the samples to EU.

    Article Title: Immediate Hypersensitivity to Polyethylene Glycols and Polysorbates: More Common Than We Have Recognized
    Article Snippet: Briefly, Corning 96-well EIA/RIA assay microplates were coated with 5,000g/mol methoxy-PEG- E.coli asparaginase (Oncaspar) at 10 µg/ml. .. For anti-IgE detection, the same plasma samples were pretreated with Protein G Plus Agarose (Thermo Fisher Scientific) at 1:1 ratio to remove IgG, then incubated at 1:10 dilution.

    Article Title: Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines
    Article Snippet: Paragraph title: Ab detection by ELISA ... For OVA- and NP-specific IgG1 or IgE detection, we incubated serially diluted sera from immunized mice on 96-well Immulon plates (Thermo Scientific) coated with OVA (grade VII, Sigma-Aldrich) at 100 μg/ml or NP at 10 μg/ml.

    Microarray:

    Article Title: Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer, et al. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer
    Article Snippet: As the human IgE standard designed for IgE detection in human sera on ISAC112 could not be used, a work‐around was developed with the team of ThermoFisher, using an alternative positive signal with horse IgE on a spotted allergen as reference and comparing all values hereto in a semiquantitative manner. .. Microarray image data were analysed by MIA (Microarray Image Analysis Software, V1.2, Thermo Fisher Scientific, Uppsala, Sweden), and the ISAC standardized units (ISU) were calculated by comparing with a calibration curve (specific IgE CTRL, Thermo Fisher Scientific, Vienna, Austria, CTR02) (Figure B ) .

    Article Title: Microarrayed dog, cat, and horse allergens show weak correlation between allergen-specific IgE and IgG responses⋆
    Article Snippet: .. For IgE detection, 30 μL of serum, or for IgG detection 30 μL of a 1:50 dilution of serum, was added to the microarray and incubated for 120 minutes, followed by washing and incubation with fluorescence-labeled anti-IgE or anti-IgG antibodies (Thermo Fisher), respectively, for 30 minutes. .. Then, chips were washed, dried, and analyzed with a Laser Scan Confocal microarray reader (LuxScan 10K/A; Capital-Bio, Beijing, China) and evaluated with Phadia Microarray Image Analysis software.

    Incubation:

    Article Title: Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer, et al. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer
    Article Snippet: All detection steps were carried out according to the manufacturer's instruction, with slight modifications: Thirty μL undiluted horse serum was incubated on ISAC131 for 2 hours, and glass slides were then washed with ThermoFisher washing fluid. .. As the human IgE standard designed for IgE detection in human sera on ISAC112 could not be used, a work‐around was developed with the team of ThermoFisher, using an alternative positive signal with horse IgE on a spotted allergen as reference and comparing all values hereto in a semiquantitative manner.

    Article Title: Antigenic determinants of Der p 1: Specificity and cross-reactivity associated with IgE antibody recognition
    Article Snippet: IgE detection and quantification in plasma samples was determined using Specific IgE detection streptavidin-ImmunoCAP on the Phadia® 100 (Thermo Fisher Scientific, Portage, MI). .. After testing several concentrations of allergen (from 0.25 to 10 µg/ml), streptavidin ImmunoCAPs were loaded with 5 µg/CAP of biotinylated allergen and incubated for 30 minutes.

    Article Title: Allergen valency, dose and FcεRI occupancy set thresholds for secretory responses to Pen a 1 and motivate design of hypoallergens
    Article Snippet: .. For IgE detection, wells were thoroughly washed and incubated with polyclonal goat anti-human IgE-HRP (Invitrogen, Grand Island, NY). .. Optical density was read at 450 nm following detection with 1-step ultra TMB-ELISA substrate (Thermofisher, MA, USA) and 2M sulfuric acid as stop solution.

    Article Title: Microarrayed dog, cat, and horse allergens show weak correlation between allergen-specific IgE and IgG responses⋆
    Article Snippet: .. For IgE detection, 30 μL of serum, or for IgG detection 30 μL of a 1:50 dilution of serum, was added to the microarray and incubated for 120 minutes, followed by washing and incubation with fluorescence-labeled anti-IgE or anti-IgG antibodies (Thermo Fisher), respectively, for 30 minutes. .. Then, chips were washed, dried, and analyzed with a Laser Scan Confocal microarray reader (LuxScan 10K/A; Capital-Bio, Beijing, China) and evaluated with Phadia Microarray Image Analysis software.

    Article Title: Immediate Hypersensitivity to Polyethylene Glycols and Polysorbates: More Common Than We Have Recognized
    Article Snippet: .. For anti-IgE detection, the same plasma samples were pretreated with Protein G Plus Agarose (Thermo Fisher Scientific) at 1:1 ratio to remove IgG, then incubated at 1:10 dilution. .. HRP-conjugated goat anti-human IgG (Sigma) or anti-human IgE (BioRad) antibodies were added at 1:1000 and 1:10,000 dilution respectively.

    Article Title: Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines
    Article Snippet: .. For OVA- and NP-specific IgG1 or IgE detection, we incubated serially diluted sera from immunized mice on 96-well Immulon plates (Thermo Scientific) coated with OVA (grade VII, Sigma-Aldrich) at 100 μg/ml or NP at 10 μg/ml. .. We detected bound IgG1 using alkaline phosphatase–conjugated anti-mouse IgG1 Abs (BD Pharmingen) followed by incubation with p -nitrophenyl phosphate and measurement by spectrophotometry.

    Modification:

    Article Title: Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure
    Article Snippet: Diagnostic performance characteristics of the standard Phadebas RAST, modified RAST, and Pharmacia CAP system versus skin testing. .. Comparison of singleplex specific IgE detection immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy.

    Western Blot:

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: Introduction There is a growing body of evidence that IgE-mediated allergic diseases have increased over the last 40 years to the point of becoming the most prevalent diseases in the Western world , . .. In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , .

    Flow Cytometry:

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , . .. A flow cytometric bead array (CBA) was developed to detect soluble factors, like cytokines or mediators, released in vivo or in vitro during spontaneous or experimentally-induced immune responses – .

    RAST Test:

    Article Title: Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure
    Article Snippet: Diagnostic performance characteristics of the standard Phadebas RAST, modified RAST, and Pharmacia CAP system versus skin testing. .. Comparison of singleplex specific IgE detection immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy.

    Single-particle Tracking:

    Article Title: Food-induced anaphylaxis: our experience
    Article Snippet: .. Detailed history and physical examination of patients, skin prick test (SPT) with food commercial extracts and with aeroallergens and panallergens LTP and profilin (Alk), prick-to-prick with fresh foods, and serum specific IgE detection (CAP, Phadia) were performed in order to confirm the causative role of the suspected food. ..

    Recombinant:

    Article Title: Allergen valency, dose and FcεRI occupancy set thresholds for secretory responses to Pen a 1 and motivate design of hypoallergens
    Article Snippet: In brief, recombinant Pen a 1 or fragments were coated on the bottom of a high-binding, flat-bottom 96-well plates (Corning Inc., Corning, N.Y., USA) at 1.25 ug/ml in carbonate-bicarbonate buffer pH 9.6. .. For IgE detection, wells were thoroughly washed and incubated with polyclonal goat anti-human IgE-HRP (Invitrogen, Grand Island, NY).

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: During the last decades the understanding about allergenic molecules has steadily increased year by year, thus resulting in a remarkable expansion in the identification, characterization and production of allergens, either natural or recombinant ( http://www.allergome.org/script/statistic.php ). .. In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , .

    Crocin Bleaching Assay:

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , . .. A flow cytometric bead array (CBA) was developed to detect soluble factors, like cytokines or mediators, released in vivo or in vitro during spontaneous or experimentally-induced immune responses – .

    Multiplexing:

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: .. In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , . .. A major criticism has been raised toward this multiplexing approach as it is felt to be too rigid in the allergen selection.

    In Vivo:

    Article Title: Two Distinct Conformations in Bet v 2 Determine Its Proteolytic Resistance to Cathepsin S
    Article Snippet: .. Patients and Sera Upon case history, positive in vivo skin prick test and in vitro IgE detection (CAP system, Thermo Fisher Scientific, Phadia AB, Uppsala, Uppland, Sweden), birch pollen allergic patients reactive to Bet v 2 were selected (n = 7). ..

    Article Title: The Influence of Recombinant Production on the Immunologic Behavior of Birch Pollen Isoallergens
    Article Snippet: .. Patients and Sera Fagales pollen-allergic patients were selected on the basis of typical case history, positive in vivo skin prick test, and in vitro IgE detection (CAP system, Phadia AB, Uppsala, Sweden). ..

    Article Title: Reshaping the Bet v 1 fold modulates TH polarization
    Article Snippet: .. Patients with Fagales pollen allergy were selected on the basis of case history, positive in vivo skin prick test, and in vitro IgE detection (CAP System; Phadia AB, Uppsala, Sweden). ..

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , . .. A flow cytometric bead array (CBA) was developed to detect soluble factors, like cytokines or mediators, released in vivo or in vitro during spontaneous or experimentally-induced immune responses – .

    Fluorescence:

    Article Title: Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer, et al. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer
    Article Snippet: After final washing, fluorescence was measured in an ISAC microchip‐reader (LUX‐Scan‐10K/A; CapitalBio Corporation, Beijing, China) with excitation at 532 nm (green laser). .. As the human IgE standard designed for IgE detection in human sera on ISAC112 could not be used, a work‐around was developed with the team of ThermoFisher, using an alternative positive signal with horse IgE on a spotted allergen as reference and comparing all values hereto in a semiquantitative manner.

    Article Title: Microarrayed dog, cat, and horse allergens show weak correlation between allergen-specific IgE and IgG responses⋆
    Article Snippet: .. For IgE detection, 30 μL of serum, or for IgG detection 30 μL of a 1:50 dilution of serum, was added to the microarray and incubated for 120 minutes, followed by washing and incubation with fluorescence-labeled anti-IgE or anti-IgG antibodies (Thermo Fisher), respectively, for 30 minutes. .. Then, chips were washed, dried, and analyzed with a Laser Scan Confocal microarray reader (LuxScan 10K/A; Capital-Bio, Beijing, China) and evaluated with Phadia Microarray Image Analysis software.

    Mouse Assay:

    Article Title: Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines
    Article Snippet: .. For OVA- and NP-specific IgG1 or IgE detection, we incubated serially diluted sera from immunized mice on 96-well Immulon plates (Thermo Scientific) coated with OVA (grade VII, Sigma-Aldrich) at 100 μg/ml or NP at 10 μg/ml. .. We detected bound IgG1 using alkaline phosphatase–conjugated anti-mouse IgG1 Abs (BD Pharmingen) followed by incubation with p -nitrophenyl phosphate and measurement by spectrophotometry.

    Chromatin Immunoprecipitation:

    Article Title: Bet v 1-like pollen allergens of multiple Fagales species can sensitize atopic individuals
    Article Snippet: .. Fagales pollen allergic patients were selected based on case history, positive skin prick test and in vitro IgE detection using Immuno Solid-phase Allergen Chip (ISAC) 103 (Phadia Multiplexing Diagnostics GMBH, Vienna, Austria) [ ] according to previously reported protocols [ ]. ..

    Article Title: Microarrayed dog, cat, and horse allergens show weak correlation between allergen-specific IgE and IgG responses⋆
    Article Snippet: Quality controls were performed with IgE and IgG standards with established IgE and IgG reactivity to allergen components on the chip. .. For IgE detection, 30 μL of serum, or for IgG detection 30 μL of a 1:50 dilution of serum, was added to the microarray and incubated for 120 minutes, followed by washing and incubation with fluorescence-labeled anti-IgE or anti-IgG antibodies (Thermo Fisher), respectively, for 30 minutes.

    Software:

    Article Title: Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer, et al. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer
    Article Snippet: As the human IgE standard designed for IgE detection in human sera on ISAC112 could not be used, a work‐around was developed with the team of ThermoFisher, using an alternative positive signal with horse IgE on a spotted allergen as reference and comparing all values hereto in a semiquantitative manner. .. Microarray image data were analysed by MIA (Microarray Image Analysis Software, V1.2, Thermo Fisher Scientific, Uppsala, Sweden), and the ISAC standardized units (ISU) were calculated by comparing with a calibration curve (specific IgE CTRL, Thermo Fisher Scientific, Vienna, Austria, CTR02) (Figure B ) .

    Article Title: Microarrayed dog, cat, and horse allergens show weak correlation between allergen-specific IgE and IgG responses⋆
    Article Snippet: For IgE detection, 30 μL of serum, or for IgG detection 30 μL of a 1:50 dilution of serum, was added to the microarray and incubated for 120 minutes, followed by washing and incubation with fluorescence-labeled anti-IgE or anti-IgG antibodies (Thermo Fisher), respectively, for 30 minutes. .. Then, chips were washed, dried, and analyzed with a Laser Scan Confocal microarray reader (LuxScan 10K/A; Capital-Bio, Beijing, China) and evaluated with Phadia Microarray Image Analysis software.

    Multiplex Assay:

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: .. In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , . .. A major criticism has been raised toward this multiplexing approach as it is felt to be too rigid in the allergen selection.

    Selection:

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , . .. A major criticism has been raised toward this multiplexing approach as it is felt to be too rigid in the allergen selection.

    In Vitro:

    Article Title: Two Distinct Conformations in Bet v 2 Determine Its Proteolytic Resistance to Cathepsin S
    Article Snippet: .. Patients and Sera Upon case history, positive in vivo skin prick test and in vitro IgE detection (CAP system, Thermo Fisher Scientific, Phadia AB, Uppsala, Uppland, Sweden), birch pollen allergic patients reactive to Bet v 2 were selected (n = 7). ..

    Article Title: The Influence of Recombinant Production on the Immunologic Behavior of Birch Pollen Isoallergens
    Article Snippet: .. Patients and Sera Fagales pollen-allergic patients were selected on the basis of typical case history, positive in vivo skin prick test, and in vitro IgE detection (CAP system, Phadia AB, Uppsala, Sweden). ..

    Article Title: Bet v 1-like pollen allergens of multiple Fagales species can sensitize atopic individuals
    Article Snippet: .. Fagales pollen allergic patients were selected based on case history, positive skin prick test and in vitro IgE detection using Immuno Solid-phase Allergen Chip (ISAC) 103 (Phadia Multiplexing Diagnostics GMBH, Vienna, Austria) [ ] according to previously reported protocols [ ]. ..

    Article Title: Reshaping the Bet v 1 fold modulates TH polarization
    Article Snippet: .. Patients with Fagales pollen allergy were selected on the basis of case history, positive in vivo skin prick test, and in vitro IgE detection (CAP System; Phadia AB, Uppsala, Sweden). ..

    Article Title: Chimeras of Bet v 1 and Api g 1 reveal heterogeneous IgE responses in patients with birch pollen allergy
    Article Snippet: .. Patients were selected on the basis of a typical case history of birch pollen allergy, positive skin prick test responses to birch pollen, and/or in vitro IgE detection to rBet v 1 or birch pollen extract (≥0.35 kUA /L; ImmunoCAP, Thermo-Fisher, Uppsala, Sweden). ..

    Article Title: Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric Immunoassay
    Article Snippet: During the last 10 years, the use of allergenic molecules immobilized on microarrays has been the most fascinating novelty for in vitro specific IgE detection , allowing at the same time both a higher definition of the patient's IgE sensitization profile and a broader evaluation of IgE reactivity in an unprecedented epidemiological study scale . .. In fact, besides the use of micro-technology for developing the novel IgE detection method, microarrays like the ISAC (Phadia Multiplexing Diagnostics, PMD, Vienna, Austria) used in the study of Scala et al. bear the intrinsic concept of multiplex testing, where multiplexing means testing a single specimen of the serum sample obtaining hundreds of results at once , .

    Spectrophotometry:

    Article Title: Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines
    Article Snippet: For OVA- and NP-specific IgG1 or IgE detection, we incubated serially diluted sera from immunized mice on 96-well Immulon plates (Thermo Scientific) coated with OVA (grade VII, Sigma-Aldrich) at 100 μg/ml or NP at 10 μg/ml. .. We detected bound IgG1 using alkaline phosphatase–conjugated anti-mouse IgG1 Abs (BD Pharmingen) followed by incubation with p -nitrophenyl phosphate and measurement by spectrophotometry.

    MicroChIP Assay:

    Article Title: Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer, et al. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer
    Article Snippet: Paragraph title: Design of ImmunoCAP allergen microchip ISAC131 ... As the human IgE standard designed for IgE detection in human sera on ISAC112 could not be used, a work‐around was developed with the team of ThermoFisher, using an alternative positive signal with horse IgE on a spotted allergen as reference and comparing all values hereto in a semiquantitative manner.

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  • 94
    Thermo Fisher anti human ig
    Anti Human Ig, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human ig/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human ig - by Bioz Stars, 2020-03
    94/100 stars
      Buy from Supplier

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