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Schematic representation of the multiplex immunoassay developed for anti-S1, anti-RBD and anti-NC <t>IgG</t> antibody detection. Anti-S1 IgG ( A ), anti-RBD IgG ( B ) and/or anti-NC IgG ( C ) antibodies present in patients’ sera will bind to the magnetic beads functionalized with each antigen, and were differentially color-coded. Human antibodies captured onto each bead by the corresponding antigen would be recognized by the biotinylated antihuman IgG detection antibody, that will in turn bind fluorescently labeled streptavidin (STV-PE). PE fluorescence corresponding to the amount of antibodies captured by each antigen will be assigned by electronically gating each fluorescently coded bead, thus allowing for the simultaneous detection of IgG antibodies recognizing S1, NC and RBD by the Bio-Plex TM array reader.
Mouse Igg2a Fc Tag, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse igg2a fc tag - by Bioz Stars, 2026-07
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mouse igg2a fc portion  (ACROBiosystems)
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ACROBiosystems mouse igg2a fc portion
Schematic representation of the multiplex immunoassay developed for anti-S1, anti-RBD and anti-NC <t>IgG</t> antibody detection. Anti-S1 IgG ( A ), anti-RBD IgG ( B ) and/or anti-NC IgG ( C ) antibodies present in patients’ sera will bind to the magnetic beads functionalized with each antigen, and were differentially color-coded. Human antibodies captured onto each bead by the corresponding antigen would be recognized by the biotinylated antihuman IgG detection antibody, that will in turn bind fluorescently labeled streptavidin (STV-PE). PE fluorescence corresponding to the amount of antibodies captured by each antigen will be assigned by electronically gating each fluorescently coded bead, thus allowing for the simultaneous detection of IgG antibodies recognizing S1, NC and RBD by the Bio-Plex TM array reader.
Mouse Igg2a Fc Portion, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
mouse igg2a fc portion - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
mouse igg2a fc tag mfc tag  (ACROBiosystems)
95
ACROBiosystems mouse igg2a fc tag mfc tag
Schematic representation of the multiplex immunoassay developed for anti-S1, anti-RBD and anti-NC <t>IgG</t> antibody detection. Anti-S1 IgG ( A ), anti-RBD IgG ( B ) and/or anti-NC IgG ( C ) antibodies present in patients’ sera will bind to the magnetic beads functionalized with each antigen, and were differentially color-coded. Human antibodies captured onto each bead by the corresponding antigen would be recognized by the biotinylated antihuman IgG detection antibody, that will in turn bind fluorescently labeled streptavidin (STV-PE). PE fluorescence corresponding to the amount of antibodies captured by each antigen will be assigned by electronically gating each fluorescently coded bead, thus allowing for the simultaneous detection of IgG antibodies recognizing S1, NC and RBD by the Bio-Plex TM array reader.
Mouse Igg2a Fc Tag Mfc Tag, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iga-m5207-1mg/pmc08553634-32-10-17?v=ACROBiosystems
Average 95 stars, based on 1 article reviews
mouse igg2a fc tag mfc tag - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier

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Schematic representation of the multiplex immunoassay developed for anti-S1, anti-RBD and anti-NC IgG antibody detection. Anti-S1 IgG ( A ), anti-RBD IgG ( B ) and/or anti-NC IgG ( C ) antibodies present in patients’ sera will bind to the magnetic beads functionalized with each antigen, and were differentially color-coded. Human antibodies captured onto each bead by the corresponding antigen would be recognized by the biotinylated antihuman IgG detection antibody, that will in turn bind fluorescently labeled streptavidin (STV-PE). PE fluorescence corresponding to the amount of antibodies captured by each antigen will be assigned by electronically gating each fluorescently coded bead, thus allowing for the simultaneous detection of IgG antibodies recognizing S1, NC and RBD by the Bio-Plex TM array reader.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: Schematic representation of the multiplex immunoassay developed for anti-S1, anti-RBD and anti-NC IgG antibody detection. Anti-S1 IgG ( A ), anti-RBD IgG ( B ) and/or anti-NC IgG ( C ) antibodies present in patients’ sera will bind to the magnetic beads functionalized with each antigen, and were differentially color-coded. Human antibodies captured onto each bead by the corresponding antigen would be recognized by the biotinylated antihuman IgG detection antibody, that will in turn bind fluorescently labeled streptavidin (STV-PE). PE fluorescence corresponding to the amount of antibodies captured by each antigen will be assigned by electronically gating each fluorescently coded bead, thus allowing for the simultaneous detection of IgG antibodies recognizing S1, NC and RBD by the Bio-Plex TM array reader.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: Multiplex Assay, Magnetic Beads, Labeling, Fluorescence

ROC analysis of the performed multiplex immunoassay. ROC curve representation (Black line) for the detection of anti-S1 IgG antibodies ( A ), anti-RBD IgG antibodies ( B ) and anti-NC IgG antibodies ( C ).

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: ROC analysis of the performed multiplex immunoassay. ROC curve representation (Black line) for the detection of anti-S1 IgG antibodies ( A ), anti-RBD IgG antibodies ( B ) and anti-NC IgG antibodies ( C ).

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: Multiplex Assay

Comparison of antibody values between samples from prepandemic and COVID-19 patients. Scatter plot represents the MFI values for anti-RBD ( A ), anti-S1 ( B ) and anti-NC IgG ( C ). Bold lines represent the assay positivity cut-off value. The p -value returned from the Mann–Whitney U test carried out between both groups is shown as asterisks (***), for p -values ≤0.001.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: Comparison of antibody values between samples from prepandemic and COVID-19 patients. Scatter plot represents the MFI values for anti-RBD ( A ), anti-S1 ( B ) and anti-NC IgG ( C ). Bold lines represent the assay positivity cut-off value. The p -value returned from the Mann–Whitney U test carried out between both groups is shown as asterisks (***), for p -values ≤0.001.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: Comparison, MANN-WHITNEY

SARS-CoV-2 IgG antibody values by age groups. Scatter plot represents the MFI values for IgG anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. No statistically significant differences are represented with ‘ns’.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: SARS-CoV-2 IgG antibody values by age groups. Scatter plot represents the MFI values for IgG anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. No statistically significant differences are represented with ‘ns’.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques:

Scatter plot showing the IgG antibody values against SARS-CoV-2 obtained in the multiplex immunoassay according to sex. Scatter plot represents the MFI values for the detection of anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. No statistically significant differences are represented with ‘ns’.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: Scatter plot showing the IgG antibody values against SARS-CoV-2 obtained in the multiplex immunoassay according to sex. Scatter plot represents the MFI values for the detection of anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. No statistically significant differences are represented with ‘ns’.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: Multiplex Assay

Dynamics of the SARS-CoV-2 antibody values according to the days since a positive PCR. Scatter plot representing the MFI values for the detection of anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. Statistical significance returned from the Mann–Whitney U test for each comparison is shown with asterisks (*), (**) or (***), for p -values ≤ 0.05, ≤0.01 and ≤0.001, respectively. No statistically significant differences are represented with ‘ns’.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: Dynamics of the SARS-CoV-2 antibody values according to the days since a positive PCR. Scatter plot representing the MFI values for the detection of anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. Statistical significance returned from the Mann–Whitney U test for each comparison is shown with asterisks (*), (**) or (***), for p -values ≤ 0.05, ≤0.01 and ≤0.001, respectively. No statistically significant differences are represented with ‘ns’.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: MANN-WHITNEY, Comparison

Comparison of the antibody values according to COVID-19 severity. Scatter plot represents the MFI values for anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. The p -values returned from the Mann–Whitney U test for each comparison is shown as asterisks (*) and (***), for p -values ≤ 0.05 and ≤0.001, respectively. No statistically significant differences are represented with ‘ns’.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: Comparison of the antibody values according to COVID-19 severity. Scatter plot represents the MFI values for anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. The p -values returned from the Mann–Whitney U test for each comparison is shown as asterisks (*) and (***), for p -values ≤ 0.05 and ≤0.001, respectively. No statistically significant differences are represented with ‘ns’.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: Comparison, MANN-WHITNEY

Comparison of different SARS-CoV-2 IgG antibody profiles by body mass index. Scatter plot represents the MFI values for anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. The p -values returned from the Mann–Whitney U test for each comparison is shown as asterisks (*), for p -values ≤ 0.05. No statistically significant differences are represented with ‘ns’.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: Comparison of different SARS-CoV-2 IgG antibody profiles by body mass index. Scatter plot represents the MFI values for anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. The p -values returned from the Mann–Whitney U test for each comparison is shown as asterisks (*), for p -values ≤ 0.05. No statistically significant differences are represented with ‘ns’.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: Comparison, MANN-WHITNEY

Comparison of antibody values according to comorbidities. Scatter plot represents the MFI values for anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. The p -values returned from the Mann–Whitney U test for each comparison is shown as asterisks (*), for p -values ≤ 0.05. No statistically significant differences are represented with ‘ns’.

Journal: Microorganisms

Article Title: Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain

doi: 10.3390/microorganisms11122997

Figure Lengend Snippet: Comparison of antibody values according to comorbidities. Scatter plot represents the MFI values for anti-S1 ( A ), anti-RBD ( B ) and anti-NC IgG ( C ) antibodies. Bold lines represent the assay positivity cut-off. The p -values returned from the Mann–Whitney U test for each comparison is shown as asterisks (*), for p -values ≤ 0.05. No statistically significant differences are represented with ‘ns’.

Article Snippet: Binding between SARS-CoV-2 S1 (ACROBiosystems SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag), RBD (ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag) and NC (ACROBiosystems SARS-CoV-2 (COVID-19) nucleocapsid protein, His Tag) recombinant proteins to the carboxylated bead surface was based on the methodology described in the Bio-Plex Pro TM magnetic COOH Beads Amine Coupling Kit (Bio-Rad, Hercules, CA, USA) instruction manual.

Techniques: Comparison, MANN-WHITNEY