Journal: Cancers

Article Title: Mitochondrial Iron Metabolism as a Potential Key Mediator of PD-L1 Thermal Regulation

doi: 10.3390/cancers16223736

Figure Lengend Snippet: Temperature-dependent cell killing of IDH-mutant and wild-type glioma cells. ( A ) U87 and U87 R132H cells were heated to 43, 46, and 50 °C for 3 and 10 min prior to being plated for clonogenic survival (left and center panels, respectively). ( B ) Relative sensitivity of U87 and U87 R132H cells when heated to 43 °C for 10 min. Error bars represent mean ± SD ( n = 6 per group) with * p < 0.05 using a two-way ANOVA test.

Article Snippet: U87 R132H cells were generated at ATCC using a c.395G>A knock-in mutation encoding IDH1-R132H protein expression.

Techniques: Mutagenesis

Journal: Cancers

Article Title: Mitochondrial Iron Metabolism as a Potential Key Mediator of PD-L1 Thermal Regulation

doi: 10.3390/cancers16223736

Figure Lengend Snippet: Effects of thermal therapy on glioma cell radiosensitivity. U87 and U87 R132H cells were heated to 43 °C for 10 min prior to being treated with 2 Gy irradiation and plated to assess colony formation. Error bars represent mean ± SD (n = 6 per group) with p -values generated using a one-way ANOVA test.

Article Snippet: U87 R132H cells were generated at ATCC using a c.395G>A knock-in mutation encoding IDH1-R132H protein expression.

Techniques: Irradiation, Generated

Journal: Cancers

Article Title: Mitochondrial Iron Metabolism as a Potential Key Mediator of PD-L1 Thermal Regulation

doi: 10.3390/cancers16223736

Figure Lengend Snippet: Thermal reversal of radiation-induced PD-L1 expression is associated with induction of mortalin. Western blot analysis of PD-L1, TfR, Ft-H, and mortalin expression in U87 and U87 R132H cells that had been heated to 43 °C for 10 min prior to being treated with 2 Gy irradiation. Uncropped western blots are provided in .

Article Snippet: U87 R132H cells were generated at ATCC using a c.395G>A knock-in mutation encoding IDH1-R132H protein expression.

Techniques: Expressing, Western Blot, Irradiation

Journal: Cancers

Article Title: Mitochondrial Iron Metabolism as a Potential Key Mediator of PD-L1 Thermal Regulation

doi: 10.3390/cancers16223736

Figure Lengend Snippet: Gallium enhances the toxicity of thermal therapy and ionizing radiation. ( A ) Clonogenic survival analysis of U87 R132H cells treated with ± 500 µM Ga(NO 3 ) 3 for 24 h prior to collection and treatment at ± 43 °C for 10 min followed by 2 Gy irradiation. Error bars represent mean ± SEM ( n = 3) where * p < 0.05 using a one-way ANOVA test with a post-hoc Tukey’s analysis for multiple comparisons. ( B ) Western blot analysis of PD-L1 cells treated with ± 500 µM Ga(NO 3 ) 3 for 24 h prior to collection and treatment ± 43 °C for 10 min, followed by 2 Gy irradiation. β-actin was used as a loading control, but due to variability in β-actin, the membrane was stained with Ponseau-S as an additional loading control (band shown for 37 kDa).

Article Snippet: U87 R132H cells were generated at ATCC using a c.395G>A knock-in mutation encoding IDH1-R132H protein expression.

Techniques: Irradiation, Western Blot, Control, Membrane, Staining

Journal: Acta Neuropathologica Communications

Article Title: Dual phenotypes in recurrent astrocytoma, IDH-mutant; coexistence of IDH-mutant and IDH-wildtype components: a case report with genetic and epigenetic analysis

doi: 10.1186/s40478-024-01879-9

Figure Lengend Snippet: Hematoxylin and eosin (HE) staining and immunohistochemistry (IHC) of the primary and the secondary recurrent tumor. ( A ) HE staining and IHC staining using anti-IDH1 R132H antibody (inset) of the primary brain tumor. ( B ) HE staining of the second recurrent tumor. The upper area of the image shows a small round-cell tumor component (arrow). Atypical glial cells with nuclear hyperchromasia and pleomorphism are observed in the lower area (arrowhead). ( C ) IHC staining of the second recurrent tumor with anti-IDH1 R132H antibody. ( D ) IHC staining of the second recurrent tumor using anti-Ki-67 antibody. All scale bars represent 100 μm

Article Snippet: Multiple stainings were performed on the adjacent section with antibodies against IDH1 R132H (Dianova GmbH, H09 clone), GFAP (Dako, 6F2 clone), p53 (Santa Cruz Biotechnology, DO-1 clone), ATRX (Sigma-Aldrich, polyclonal), and Ki-67 (Dako, Mib-1 clone).

Techniques: Staining, Immunohistochemistry

Journal: Acta Neuropathologica Communications

Article Title: Dual phenotypes in recurrent astrocytoma, IDH-mutant; coexistence of IDH-mutant and IDH-wildtype components: a case report with genetic and epigenetic analysis

doi: 10.1186/s40478-024-01879-9

Figure Lengend Snippet: Molecular profiling of NGY-P, NGY-R1, NGY-R2A, and NGY-R2B. ( A ) A phylogeny tree depicts the optimal evolution pattern of primary and recurrent samples highlighting genes that are frequently mutated in tumors. Branch lengths are proportional to the number of mutations detected. ( B ) This panels shows the β allele frequency of chromosome 2 for each sample, where the IDH1 are located. ( C ) Kaplan–Meier curves are used to represent cases with CDKN2A homozygous deletion (HD) and PDGFR amplification (amp; n = 5), as well as cases with CDKN2A HD ( n = 9) among IDH-mutant astrocytoma. The X-axis indicates time (months) and the Y-axis indicates probability of survival. ( D ) MGMT promoter methylation status of NGY-R2A and NGY-R2B. The y-axis represents MGMT promoter methylation score, and the red dot line represents cut off value (0.3582). ( E ) t-distributed stochastic neighbor embedding (t-SNE) plot is drawn from the methylation data of our cases and reference data, which includes low-grade and high-grade gliomas. A IDH, IDH-mutant astrocytoma ( n = 78); A IDH HG, high-grade IDH-mutant astrocytoma ( n = 46); and O IDH, IDH-mutant oligodendroglioma ( n = 80). Reference methylation data for gliomas (GSE90496) were obtained from the Gene Expression Omnibus database ( http://www.ncbi.nlm.nih.gov/geo/ ). A molecular classification algorithm and copy number analysis from the German Cancer Center (DKFZ classifier, https://www.molecularneuropathology.org/mnp ) was performed . GBM, MES ( n = 56), MID ( n = 14), MYCN ( n = 16), RTK1 ( n = 64), RTK2 ( n = 143), RTK3 ( n = 13), and G34 ( n = 34) represent GBM subgroups

Article Snippet: Multiple stainings were performed on the adjacent section with antibodies against IDH1 R132H (Dianova GmbH, H09 clone), GFAP (Dako, 6F2 clone), p53 (Santa Cruz Biotechnology, DO-1 clone), ATRX (Sigma-Aldrich, polyclonal), and Ki-67 (Dako, Mib-1 clone).

Techniques: Amplification, Mutagenesis, Methylation, Expressing