Journal: Journal of Korean Neurosurgical Society
Article Title: Analysis of Mutant Isocitrate Dehydrogenase 1 Immunoexpression, Ki-67 and Programmed Death Ligand 1 in Diffuse Astrocytic Tumours : Study of Single Center in Bandung, Indonesia
doi: 10.3340/jkns.2020.0071
Figure Lengend Snippet: Immunohistochemical detection of gliomas cells profile. Representative image of a glioma specimen that all cells positively stained with GFAP (A); high proliferation gliomas cells stained with Ki-67 (B), mutant IDH1 R132H positive (C) and negative (D); PD-L1 positive 50–80% (E) and positive >80% (F) are shown. Original magnification : ×100; scale bar : 50 μm. GFAP : glial fibrillary acidic protein, IDH : isocitrate dehydrogenase, PD-L1 : programmed death ligand 1.
Article Snippet: After the initial processing step, sections were incubated with primary antibodies anti-human glial fibrillary acidic protein (GFAP) AB (CP 040 Biocare, Clone N/A, rabbit monoclonal AB, at 1 : 150 dilution); anti-human Ki-67 AB (Cellmarquee, Clone SP6, rabbit monoclonal AB, at 1 : 200 dilution); anti-human IDH1 R132H mutant specific AB (GTX57185 Genetex, IHC 132, mouse monoclonal AB, at 1 : 200 dilution); anti-human PD-L1 AB (ab205921 Abcam, clone 28-8, rabbit monoclonal AB, at 1 : 200 dilution) at the room temperature, respectively; followed by incubation with polyHRP non-biotin detection system for 30 minutes.
Techniques: Immunohistochemical staining, Staining, Mutagenesis