icon protein concentrators  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher icon protein concentrators
    Icon Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icon protein concentrators/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    icon protein concentrators - by Bioz Stars, 2020-04
    86/100 stars

    Related Products / Commonly Used Together

    tris-hcl
    edta
    buffer-exchanged
    t75 flasks
    gncl

    Images

    Related Articles

    Transfection:

    Article Title: Insulin-like Growth Factor Binding Protein-3 Expression in the Human Corneal Epithelium
    Article Snippet: For protein analysis from conditioned media, media was collected from T75 flasks after 48 hours and concentrated using iCON Protein Concentrators (Pierce, Rockford, IL). .. Cells transfected with pcDNA-IGFBP3, a plasmid encoding the cDNA for IGFBP3 (a gift of Dr. Sue Firth, the Kolling Institue, Sydney, Australia), and 0.5 μg rhIGFBP3 were used as a positive control.

    Positive Control:

    Article Title: Insulin-like Growth Factor Binding Protein-3 Expression in the Human Corneal Epithelium
    Article Snippet: For protein analysis from conditioned media, media was collected from T75 flasks after 48 hours and concentrated using iCON Protein Concentrators (Pierce, Rockford, IL). .. Cells transfected with pcDNA-IGFBP3, a plasmid encoding the cDNA for IGFBP3 (a gift of Dr. Sue Firth, the Kolling Institue, Sydney, Australia), and 0.5 μg rhIGFBP3 were used as a positive control.

    Protease Inhibitor:

    Article Title: Insulin-like Growth Factor Binding Protein-3 Expression in the Human Corneal Epithelium
    Article Snippet: Epithelial cell lysates were collected by lysing cells directly in plastic 6-well culture dishes using RIPA buffer containing a protease inhibitor cocktail tablet (Complete-Mini, Roche Diagnostics, Indianapolis, IN) on ice for 10 minutes. .. For protein analysis from conditioned media, media was collected from T75 flasks after 48 hours and concentrated using iCON Protein Concentrators (Pierce, Rockford, IL).

    Cell Culture:

    Article Title: Elevated IGFBP3 levels in diabetic tears: a negative regulator of IGF-1 signaling in the corneal epithelium
    Article Snippet: Conditioned media samples were concentrated using iCON protein concentrators (Pierce, Rockford, IL). .. To detect phosphorylated IGF-1R, 3×105 cultured cells were starved in basal media for 24 hours and then stimulated by 25 ng/ml of recombinant human IGF-1 (BioVision Inc, Mountain View, California) with or without recombinant human IGFBP-3 (BioVision Inc, Mountain View, California) for 15 minutes at IGFBP3:IGF-1 ratios that simulated the ratios detected in normal and diabetic human tears.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Elevated IGFBP3 levels in diabetic tears: a negative regulator of IGF-1 signaling in the corneal epithelium
    Article Snippet: Paragraph title: ELISA ... Conditioned media samples were concentrated using iCON protein concentrators (Pierce, Rockford, IL).

    Concentration Assay:

    Article Title: Structural analysis of a therapeutic monoclonal antibody dimer by hydroxyl radical footprinting
    Article Snippet: Samples were initially buffer-exchanged into 100 mM Tris-HCl pH 8.0, 7.2 M GnCl (guanidine hydrochloride), 20 mM EDTA using iCON Protein Concentrators (Pierce). .. Subsequently, samples were incubated at 37°C for one hour after addition of DTT to a final concentration of 10 mM, then at ambient temperature in the dark for 30 minutes after addition of sodium iodoacetate to a final concentration of 25 mM, and finally for 15 minutes in the dark after addition of DTT to final concentration of 50 mM.

    Incubation:

    Article Title: Structural analysis of a therapeutic monoclonal antibody dimer by hydroxyl radical footprinting
    Article Snippet: Samples were initially buffer-exchanged into 100 mM Tris-HCl pH 8.0, 7.2 M GnCl (guanidine hydrochloride), 20 mM EDTA using iCON Protein Concentrators (Pierce). .. Subsequently, samples were incubated at 37°C for one hour after addition of DTT to a final concentration of 10 mM, then at ambient temperature in the dark for 30 minutes after addition of sodium iodoacetate to a final concentration of 25 mM, and finally for 15 minutes in the dark after addition of DTT to final concentration of 50 mM.

    Western Blot:

    Article Title: Insulin-like Growth Factor Binding Protein-3 Expression in the Human Corneal Epithelium
    Article Snippet: Paragraph title: SDS PAGE and Western Blotting ... For protein analysis from conditioned media, media was collected from T75 flasks after 48 hours and concentrated using iCON Protein Concentrators (Pierce, Rockford, IL).

    Recombinant:

    Article Title: Elevated IGFBP3 levels in diabetic tears: a negative regulator of IGF-1 signaling in the corneal epithelium
    Article Snippet: Conditioned media samples were concentrated using iCON protein concentrators (Pierce, Rockford, IL). .. To detect phosphorylated IGF-1R, 3×105 cultured cells were starved in basal media for 24 hours and then stimulated by 25 ng/ml of recombinant human IGF-1 (BioVision Inc, Mountain View, California) with or without recombinant human IGFBP-3 (BioVision Inc, Mountain View, California) for 15 minutes at IGFBP3:IGF-1 ratios that simulated the ratios detected in normal and diabetic human tears.

    Article Title: Structural analysis of a therapeutic monoclonal antibody dimer by hydroxyl radical footprinting
    Article Snippet: Samples were initially buffer-exchanged into 100 mM Tris-HCl pH 8.0, 7.2 M GnCl (guanidine hydrochloride), 20 mM EDTA using iCON Protein Concentrators (Pierce). .. Samples were digested at 37°C with 3% trypsin (w/w, Roche recombinant) for four hours.

    SDS Page:

    Article Title: Insulin-like Growth Factor Binding Protein-3 Expression in the Human Corneal Epithelium
    Article Snippet: Paragraph title: SDS PAGE and Western Blotting ... For protein analysis from conditioned media, media was collected from T75 flasks after 48 hours and concentrated using iCON Protein Concentrators (Pierce, Rockford, IL).

    Plasmid Preparation:

    Article Title: Insulin-like Growth Factor Binding Protein-3 Expression in the Human Corneal Epithelium
    Article Snippet: For protein analysis from conditioned media, media was collected from T75 flasks after 48 hours and concentrated using iCON Protein Concentrators (Pierce, Rockford, IL). .. Cells transfected with pcDNA-IGFBP3, a plasmid encoding the cDNA for IGFBP3 (a gift of Dr. Sue Firth, the Kolling Institue, Sydney, Australia), and 0.5 μg rhIGFBP3 were used as a positive control.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher icon concentrator
    Icon Concentrator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icon concentrator/product/Thermo Fisher
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    icon concentrator - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    80
    Thermo Fisher mwco icon pierce concentrators
    Mwco Icon Pierce Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mwco icon pierce concentrators/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mwco icon pierce concentrators - by Bioz Stars, 2020-04
    80/100 stars
      Buy from Supplier

    Image Search Results