Structured Review

Diaclone icam 1
Effect of Digoxin (50 nM or 100 nM) on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with Digoxin for one hour, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and <t>ICAM-1</t> (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 3 experiments with cells from different donors are shown.
Icam 1, supplied by Diaclone, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Effects of TLR Agonists on the Hypoxia-Regulated Transcription Factor HIF-1? and Dendritic Cell Maturation under Normoxic Conditions"

Article Title: Effects of TLR Agonists on the Hypoxia-Regulated Transcription Factor HIF-1? and Dendritic Cell Maturation under Normoxic Conditions

Journal: PLoS ONE

doi: 10.1371/journal.pone.0010983

Effect of Digoxin (50 nM or 100 nM) on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with Digoxin for one hour, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 3 experiments with cells from different donors are shown.
Figure Legend Snippet: Effect of Digoxin (50 nM or 100 nM) on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with Digoxin for one hour, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 3 experiments with cells from different donors are shown.

Techniques Used: Isolation, Cell Culture, Incubation, Expressing, Fluorescence

Effect of YC-1 on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with YC-1 (100 µM) for 5 min, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA µ20 (g/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 4 experiments with cells from different donors are shown. *p
Figure Legend Snippet: Effect of YC-1 on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with YC-1 (100 µM) for 5 min, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA µ20 (g/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 4 experiments with cells from different donors are shown. *p

Techniques Used: Isolation, Cell Culture, Incubation, Expressing, Fluorescence

Effect of CTM on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. Inhibition of HIF-1α expression by CTM inhibited maturation of MoDC in response to LPS. MoDC were incubated with CTM (200 nM) for 3 hours, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 6 experiments with cells from different donors are shown. *p
Figure Legend Snippet: Effect of CTM on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. Inhibition of HIF-1α expression by CTM inhibited maturation of MoDC in response to LPS. MoDC were incubated with CTM (200 nM) for 3 hours, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 6 experiments with cells from different donors are shown. *p

Techniques Used: Isolation, Cell Culture, Inhibition, Expressing, Incubation, Fluorescence

2) Product Images from "The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium"

Article Title: The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium

Journal: Oncotarget

doi: 10.18632/oncotarget.21240

In healthy volunteers, aspirin 300 mg administered for 28 days led to a reduction in serum prostaglandin E2 (PGE2; Panel B ) and netrin-1 (Panel C) , as well as thromboxane B2 (TXB2; Panel A ). The reduction in netrin-1 was associated with increased serum salicylate levels (Panel E) , while there was a positive correlation between netrin-1 and PGE 2 levels (Panel F) . There was no change in markers of endothelial dysfunction, vascular cell adhesion molecule-1 (VCAM-1; Panel G ), intracellular adhesion molecule (ICAM-1; Panel H ), or E-selectin ( Panel I ). High-sensitivity C-reactive protein (hs-CRP) levels remained unchanged post treatment (Panel J) . Western blotting was performed to identify both the full length, 70 kDa netrin-1 protein and the truncated, 55 kDa netrin-1 protein, but only the truncated isoform was detected (Panel D) .
Figure Legend Snippet: In healthy volunteers, aspirin 300 mg administered for 28 days led to a reduction in serum prostaglandin E2 (PGE2; Panel B ) and netrin-1 (Panel C) , as well as thromboxane B2 (TXB2; Panel A ). The reduction in netrin-1 was associated with increased serum salicylate levels (Panel E) , while there was a positive correlation between netrin-1 and PGE 2 levels (Panel F) . There was no change in markers of endothelial dysfunction, vascular cell adhesion molecule-1 (VCAM-1; Panel G ), intracellular adhesion molecule (ICAM-1; Panel H ), or E-selectin ( Panel I ). High-sensitivity C-reactive protein (hs-CRP) levels remained unchanged post treatment (Panel J) . Western blotting was performed to identify both the full length, 70 kDa netrin-1 protein and the truncated, 55 kDa netrin-1 protein, but only the truncated isoform was detected (Panel D) .

Techniques Used: Western Blot

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Increased proinflammatory endothelial response to S100A8/A9 after preactivation through advanced glycation end products
Article Snippet: .. ELISA ELISA kits for IL-6, ICAM-1 and VCAM-1 were purchased from Diaclone (Besancon, France), ELISA for MCP-1 from Biosource (CA, USA). .. NF-kappaB ELISA Both preparation of nuclear extracts by the mini extraction method and measurement of NF-kappa B on microtiter plates employing anti-NFκB c-Rel (Santa Cruz Biotechnology, CA, USA) was performed according to Schreiber[ ].

Article Title: Potential anti-inflammatory, anti-adhesive, anti/estrogenic, and angiotensin-converting enzyme inhibitory activities of anthocyanins and their gut metabolites
Article Snippet: .. The secretion of MCP-1, ICAM-1, and VCAM-1 by Ea.hy 926 cells was measured using commercially available ELISA kits (Diaclone, Bionova scientific). .. ACE-inhibitory activity was measured by fluorescence using the method of Sentandreu and Toldrá ( ) with some modifications.

Article Title: Impact of high serum Immunoglobulin E levels on the risk of atherosclerosis in humans
Article Snippet: .. Serum concentrations of total IgE (Siemens, Munich, Germany), intercellular adhesion molecule-1 (ICAM-1; Diaclone SAS, Besancon Cedex, France), vascular cell adhesion molecule-1 (VCAM-1; Diaclone SAS, Besancon Cedex, France), IL-6 (Diaclone SAS, Besancon Cedex, France), endothelin-1 (Biomedica Medizinprodukte GmbH & Co KG, Wien, Austria), and hsCRP (DRG international Inc., Springfield Township, NJ, USA) were determined by enzyme-linked immunosorbent assay. .. Coronary flow measurements Coronary flow reserve (CFR) recordings were performed with a Vivid 7 echocardiography device (General Electrics, Horten, Norway) using a middle-range frequency (3 to 8 MHz) broadband transducer.

Article Title: Effects of Parenteral Vitamin D on the Biomarkers of the Endothelial Function in Patients with Type 2 Diabetes and Ischemic Heart Disease: A Randomized Clinical Trial
Article Snippet: .. The level of ICAM-1 was measured by enzyme immunometric assay using a commercial kit (Human CD54/ ICAM-1, ELISA, Diaclone, France) and a Sunrise ELISA reader (Tecan Co. Salzburg, Austria); the coefficient of variation of the intra- and inter-assay was 2.7% and 3.9%, respectively. ..

FACS:

Article Title: Effects of TLR Agonists on the Hypoxia-Regulated Transcription Factor HIF-1? and Dendritic Cell Maturation under Normoxic Conditions
Article Snippet: .. FACS analysis and cell viability Cells were incubated with FITC- or PE-labeled monoclonal antibody (mAb) against CD80, CD86 (BD, Franklin Lakes, NJ, USA), isotype control IgG1 (BD), or unlabeled mAb against CD40, ICAM-1 (Diaclone, Besançon, France) followed by a FITC-labeled polyclonal goat anti-mouse IgG (Sigma). .. For determination of viability, the cells were stained with 5 µg/ml of propidium iodide (PI; Invitrogen) and analyzed by flow cytometry.

Incubation:

Article Title: Effects of TLR Agonists on the Hypoxia-Regulated Transcription Factor HIF-1? and Dendritic Cell Maturation under Normoxic Conditions
Article Snippet: .. FACS analysis and cell viability Cells were incubated with FITC- or PE-labeled monoclonal antibody (mAb) against CD80, CD86 (BD, Franklin Lakes, NJ, USA), isotype control IgG1 (BD), or unlabeled mAb against CD40, ICAM-1 (Diaclone, Besançon, France) followed by a FITC-labeled polyclonal goat anti-mouse IgG (Sigma). .. For determination of viability, the cells were stained with 5 µg/ml of propidium iodide (PI; Invitrogen) and analyzed by flow cytometry.

Inter Assay:

Article Title: Effects of Parenteral Vitamin D on the Biomarkers of the Endothelial Function in Patients with Type 2 Diabetes and Ischemic Heart Disease: A Randomized Clinical Trial
Article Snippet: .. The level of ICAM-1 was measured by enzyme immunometric assay using a commercial kit (Human CD54/ ICAM-1, ELISA, Diaclone, France) and a Sunrise ELISA reader (Tecan Co. Salzburg, Austria); the coefficient of variation of the intra- and inter-assay was 2.7% and 3.9%, respectively. ..

Enzyme Immunoassay:

Article Title: Inhaled and systemic corticosteroid response in severe asthma assessed by alveolar nitric oxide: a randomized crossover pilot study of add-on therapy
Article Snippet: .. E-selectin and ICAM-1 were measured through use of enzyme immunoassays (Diaclone, Besancon, France; CV 1.5% and 2.8%). ..

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    Diaclone soluble icam 1
    Trio and Rac1 activity are required for TNF-α-induced <t>ICAM-1</t> and VCAM-1 expression. ( A ) Trio and Vav2 activation following TNF-α treatment was examined by means of pull-down assays with GST-Rac1-G15A mutants as described in Methods
    Soluble Icam 1, supplied by Diaclone, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Diaclone icam 1
    Effect of Digoxin (50 nM or 100 nM) on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with Digoxin for one hour, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and <t>ICAM-1</t> (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 3 experiments with cells from different donors are shown.
    Icam 1, supplied by Diaclone, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icam 1/product/Diaclone
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    icam 1 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    88
    Diaclone intracellular adhesion molecule 1
    Responses of individual cultures of fibroblast-like synoviocytes from rheumatoid arthritis (RA; n = 8) and arthritis control (AC; n = 3) patients to their autologous synovial microparticles. All individual patient data for the markers studied are expressed as the concentration of the mediator in the presence of microparticles concentrated either onefold (black bars) or threefold (open bars) divided by the concentration of mediator in the presence of microparticle-free synovial fluid. ICAM-1, intracellular adhesion <t>molecule-1;</t> MCP-1, monocyte chemoattractant protein-1.
    Intracellular Adhesion Molecule 1, supplied by Diaclone, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intracellular adhesion molecule 1/product/Diaclone
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Trio and Rac1 activity are required for TNF-α-induced ICAM-1 and VCAM-1 expression. ( A ) Trio and Vav2 activation following TNF-α treatment was examined by means of pull-down assays with GST-Rac1-G15A mutants as described in Methods

    Journal: Biology Open

    Article Title: The Rho-GEF Trio regulates a novel pro-inflammatory pathway through the transcription factor Ets2

    doi: 10.1242/bio.20134382

    Figure Lengend Snippet: Trio and Rac1 activity are required for TNF-α-induced ICAM-1 and VCAM-1 expression. ( A ) Trio and Vav2 activation following TNF-α treatment was examined by means of pull-down assays with GST-Rac1-G15A mutants as described in Methods

    Article Snippet: ELISA for sICAM-1 Trio-silenced or control HUVECs were cultured on fibronectin-coated 12-well plates and were treated with TNF-α for 20 h. Supernatant was harvested and the ELISA for soluble ICAM-1 was performed according to the manufacturer's protocol (Diaclone, Sanquin Reagentia, Amsterdam, The Netherlands).

    Techniques: Activity Assay, Expressing, Activation Assay

    The small GTPase Rac1 is activated by TNF-α and regulates the expression of VCAM-1 and ICAM-1. HUVEC were treated with TNF-α as indicated and a Rac1.GTP ( A ), RhoG.GTP ( B ) and CDC42.GTP ( C ) pull-down assay was performed as described in Online Methods. Second and third panels show protein loading. Graphs on the right show quantification. All experiments described above were repeated at least four times. Data are mean±SD. * P

    Journal: Biology Open

    Article Title: The Rho-GEF Trio regulates a novel pro-inflammatory pathway through the transcription factor Ets2

    doi: 10.1242/bio.20134382

    Figure Lengend Snippet: The small GTPase Rac1 is activated by TNF-α and regulates the expression of VCAM-1 and ICAM-1. HUVEC were treated with TNF-α as indicated and a Rac1.GTP ( A ), RhoG.GTP ( B ) and CDC42.GTP ( C ) pull-down assay was performed as described in Online Methods. Second and third panels show protein loading. Graphs on the right show quantification. All experiments described above were repeated at least four times. Data are mean±SD. * P

    Article Snippet: ELISA for sICAM-1 Trio-silenced or control HUVECs were cultured on fibronectin-coated 12-well plates and were treated with TNF-α for 20 h. Supernatant was harvested and the ELISA for soluble ICAM-1 was performed according to the manufacturer's protocol (Diaclone, Sanquin Reagentia, Amsterdam, The Netherlands).

    Techniques: Expressing, Pull Down Assay

    TrioN partially rescues TNF-α-induced VCAM-1 expression in Trio-deficient cells. ( A ) Trio expression was reduced in endothelial cells with shTrio5, resulting in impaired VCAM-1 and to a minor extent ICAM-1 expression after TNF-α treatment. GFP-TrioN was expressed in HUVEC by adenoviral delivery, as described in Online Methods. Transduction with adenovirally-delivered GFP was used as a control. Actin expression is shown for protein loading. ( B ) Graphs show quantification of VCAM-1 expression rescue by TrioN in Trio-deficient cells. The experiment was carried out four times. Data are mean±SEM. * P

    Journal: Biology Open

    Article Title: The Rho-GEF Trio regulates a novel pro-inflammatory pathway through the transcription factor Ets2

    doi: 10.1242/bio.20134382

    Figure Lengend Snippet: TrioN partially rescues TNF-α-induced VCAM-1 expression in Trio-deficient cells. ( A ) Trio expression was reduced in endothelial cells with shTrio5, resulting in impaired VCAM-1 and to a minor extent ICAM-1 expression after TNF-α treatment. GFP-TrioN was expressed in HUVEC by adenoviral delivery, as described in Online Methods. Transduction with adenovirally-delivered GFP was used as a control. Actin expression is shown for protein loading. ( B ) Graphs show quantification of VCAM-1 expression rescue by TrioN in Trio-deficient cells. The experiment was carried out four times. Data are mean±SEM. * P

    Article Snippet: ELISA for sICAM-1 Trio-silenced or control HUVECs were cultured on fibronectin-coated 12-well plates and were treated with TNF-α for 20 h. Supernatant was harvested and the ELISA for soluble ICAM-1 was performed according to the manufacturer's protocol (Diaclone, Sanquin Reagentia, Amsterdam, The Netherlands).

    Techniques: Expressing, Transduction

    Trio silencing reduces inflammatory cytokine-induced expression of ICAM-1 and VCAM-1 and reduces leukocyte adhesion and transendothelial migration. ( A ) Five lentivirally-delivered shRNA constructs targeting Trio expression were examined for their potential to downregulate Trio protein expression. The GEF Vav2 and actin are shown as loading controls. ( B ) The effect of Trio silencing on the F-actin cytoskeleton in HUVEC that were stimulated with TNF-α. Immunofluorescent images show actin in green and nuclei in blue. Scale bars: 20 µm. ( C ) ICAM-1, VCAM-1 and E-selectin protein expression upon TNF-α time-course in shCTRL and shTrio-treated HUVEC. ( D ) ICAM-1, VCAM-1 and E-selectin mRNA levels by qRT-PCR in shCTRL and shTrio-HUVEC after TNF-α. Adhesion molecule mRNA levels in shTrio-treated HUVEC as a percentage of the adhesion molecule mRNA levels in controls. Data are means of three independent experiments ± SEM. ( E ) Upregulation of VCAM-1 (left graphs) and ICAM-1 (right graphs) by TNF-α, IL-1β, IFN-γ and LPS in shCTRL (open bars) and shTrio (closed bars)-treated HUVEC. Primary monocytes were perfused over HUVEC and adhesion ( F ) and TEM ( G ) was quantified as described in Materials and Methods . This experiment was carried out four times in duplicates. Data presented in all graphs are means±SEM. * P

    Journal: Biology Open

    Article Title: The Rho-GEF Trio regulates a novel pro-inflammatory pathway through the transcription factor Ets2

    doi: 10.1242/bio.20134382

    Figure Lengend Snippet: Trio silencing reduces inflammatory cytokine-induced expression of ICAM-1 and VCAM-1 and reduces leukocyte adhesion and transendothelial migration. ( A ) Five lentivirally-delivered shRNA constructs targeting Trio expression were examined for their potential to downregulate Trio protein expression. The GEF Vav2 and actin are shown as loading controls. ( B ) The effect of Trio silencing on the F-actin cytoskeleton in HUVEC that were stimulated with TNF-α. Immunofluorescent images show actin in green and nuclei in blue. Scale bars: 20 µm. ( C ) ICAM-1, VCAM-1 and E-selectin protein expression upon TNF-α time-course in shCTRL and shTrio-treated HUVEC. ( D ) ICAM-1, VCAM-1 and E-selectin mRNA levels by qRT-PCR in shCTRL and shTrio-HUVEC after TNF-α. Adhesion molecule mRNA levels in shTrio-treated HUVEC as a percentage of the adhesion molecule mRNA levels in controls. Data are means of three independent experiments ± SEM. ( E ) Upregulation of VCAM-1 (left graphs) and ICAM-1 (right graphs) by TNF-α, IL-1β, IFN-γ and LPS in shCTRL (open bars) and shTrio (closed bars)-treated HUVEC. Primary monocytes were perfused over HUVEC and adhesion ( F ) and TEM ( G ) was quantified as described in Materials and Methods . This experiment was carried out four times in duplicates. Data presented in all graphs are means±SEM. * P

    Article Snippet: ELISA for sICAM-1 Trio-silenced or control HUVECs were cultured on fibronectin-coated 12-well plates and were treated with TNF-α for 20 h. Supernatant was harvested and the ELISA for soluble ICAM-1 was performed according to the manufacturer's protocol (Diaclone, Sanquin Reagentia, Amsterdam, The Netherlands).

    Techniques: Expressing, Migration, shRNA, Construct, Quantitative RT-PCR, Transmission Electron Microscopy

    Effect of Digoxin (50 nM or 100 nM) on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with Digoxin for one hour, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 3 experiments with cells from different donors are shown.

    Journal: PLoS ONE

    Article Title: Effects of TLR Agonists on the Hypoxia-Regulated Transcription Factor HIF-1? and Dendritic Cell Maturation under Normoxic Conditions

    doi: 10.1371/journal.pone.0010983

    Figure Lengend Snippet: Effect of Digoxin (50 nM or 100 nM) on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with Digoxin for one hour, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 3 experiments with cells from different donors are shown.

    Article Snippet: FACS analysis and cell viability Cells were incubated with FITC- or PE-labeled monoclonal antibody (mAb) against CD80, CD86 (BD, Franklin Lakes, NJ, USA), isotype control IgG1 (BD), or unlabeled mAb against CD40, ICAM-1 (Diaclone, Besançon, France) followed by a FITC-labeled polyclonal goat anti-mouse IgG (Sigma).

    Techniques: Isolation, Cell Culture, Incubation, Expressing, Fluorescence

    Effect of YC-1 on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with YC-1 (100 µM) for 5 min, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA µ20 (g/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 4 experiments with cells from different donors are shown. *p

    Journal: PLoS ONE

    Article Title: Effects of TLR Agonists on the Hypoxia-Regulated Transcription Factor HIF-1? and Dendritic Cell Maturation under Normoxic Conditions

    doi: 10.1371/journal.pone.0010983

    Figure Lengend Snippet: Effect of YC-1 on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. MoDC were incubated with YC-1 (100 µM) for 5 min, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA µ20 (g/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 4 experiments with cells from different donors are shown. *p

    Article Snippet: FACS analysis and cell viability Cells were incubated with FITC- or PE-labeled monoclonal antibody (mAb) against CD80, CD86 (BD, Franklin Lakes, NJ, USA), isotype control IgG1 (BD), or unlabeled mAb against CD40, ICAM-1 (Diaclone, Besançon, France) followed by a FITC-labeled polyclonal goat anti-mouse IgG (Sigma).

    Techniques: Isolation, Cell Culture, Incubation, Expressing, Fluorescence

    Effect of CTM on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. Inhibition of HIF-1α expression by CTM inhibited maturation of MoDC in response to LPS. MoDC were incubated with CTM (200 nM) for 3 hours, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 6 experiments with cells from different donors are shown. *p

    Journal: PLoS ONE

    Article Title: Effects of TLR Agonists on the Hypoxia-Regulated Transcription Factor HIF-1? and Dendritic Cell Maturation under Normoxic Conditions

    doi: 10.1371/journal.pone.0010983

    Figure Lengend Snippet: Effect of CTM on phenotypic maturation of MoDC. Monocytes isolated from buffy coat were cultured in the presence of GM-CSF and IL-4 for 6 days. Inhibition of HIF-1α expression by CTM inhibited maturation of MoDC in response to LPS. MoDC were incubated with CTM (200 nM) for 3 hours, afterwards cells were stimulated for 24 hours with LPS (1 µg/ml), HA (20 µg/ml) and LTA (5 µg/ml). Cells were washed and analyzed for the expression of CD40 (A), CD80 (B), CD86 (C) and ICAM-1 (D). Y-axis shows the median fluorescence intensity (MFI). Mean values ± SD of 6 experiments with cells from different donors are shown. *p

    Article Snippet: FACS analysis and cell viability Cells were incubated with FITC- or PE-labeled monoclonal antibody (mAb) against CD80, CD86 (BD, Franklin Lakes, NJ, USA), isotype control IgG1 (BD), or unlabeled mAb against CD40, ICAM-1 (Diaclone, Besançon, France) followed by a FITC-labeled polyclonal goat anti-mouse IgG (Sigma).

    Techniques: Isolation, Cell Culture, Inhibition, Expressing, Incubation, Fluorescence

    In healthy volunteers, aspirin 300 mg administered for 28 days led to a reduction in serum prostaglandin E2 (PGE2; Panel B ) and netrin-1 (Panel C) , as well as thromboxane B2 (TXB2; Panel A ). The reduction in netrin-1 was associated with increased serum salicylate levels (Panel E) , while there was a positive correlation between netrin-1 and PGE 2 levels (Panel F) . There was no change in markers of endothelial dysfunction, vascular cell adhesion molecule-1 (VCAM-1; Panel G ), intracellular adhesion molecule (ICAM-1; Panel H ), or E-selectin ( Panel I ). High-sensitivity C-reactive protein (hs-CRP) levels remained unchanged post treatment (Panel J) . Western blotting was performed to identify both the full length, 70 kDa netrin-1 protein and the truncated, 55 kDa netrin-1 protein, but only the truncated isoform was detected (Panel D) .

    Journal: Oncotarget

    Article Title: The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium

    doi: 10.18632/oncotarget.21240

    Figure Lengend Snippet: In healthy volunteers, aspirin 300 mg administered for 28 days led to a reduction in serum prostaglandin E2 (PGE2; Panel B ) and netrin-1 (Panel C) , as well as thromboxane B2 (TXB2; Panel A ). The reduction in netrin-1 was associated with increased serum salicylate levels (Panel E) , while there was a positive correlation between netrin-1 and PGE 2 levels (Panel F) . There was no change in markers of endothelial dysfunction, vascular cell adhesion molecule-1 (VCAM-1; Panel G ), intracellular adhesion molecule (ICAM-1; Panel H ), or E-selectin ( Panel I ). High-sensitivity C-reactive protein (hs-CRP) levels remained unchanged post treatment (Panel J) . Western blotting was performed to identify both the full length, 70 kDa netrin-1 protein and the truncated, 55 kDa netrin-1 protein, but only the truncated isoform was detected (Panel D) .

    Article Snippet: Enzyme-linked immunosorbent assays Enzyme-linked immunosorbent assays (ELISAs) were carried out to measure serum levels of netrin-1 (SEB827Hu, Cloud-Clone Corp., China), PGE2 (MBS007171, MyBioSource, Inc., USA), TXB2 (CSB-E08046h, Cusabio, China), VCAM-1 (DVC00, R & D Systems, UK), ICAM-1 (850.540.096, Diaclone, France) and E-selectin (CSB-E04540h, Cusabio, China).

    Techniques: Western Blot

    Responses of individual cultures of fibroblast-like synoviocytes from rheumatoid arthritis (RA; n = 8) and arthritis control (AC; n = 3) patients to their autologous synovial microparticles. All individual patient data for the markers studied are expressed as the concentration of the mediator in the presence of microparticles concentrated either onefold (black bars) or threefold (open bars) divided by the concentration of mediator in the presence of microparticle-free synovial fluid. ICAM-1, intracellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1.

    Journal: Arthritis Research & Therapy

    Article Title: Synovial microparticles from arthritic patients modulate chemokine and cytokine release by synoviocytes

    doi: 10.1186/ar1706

    Figure Lengend Snippet: Responses of individual cultures of fibroblast-like synoviocytes from rheumatoid arthritis (RA; n = 8) and arthritis control (AC; n = 3) patients to their autologous synovial microparticles. All individual patient data for the markers studied are expressed as the concentration of the mediator in the presence of microparticles concentrated either onefold (black bars) or threefold (open bars) divided by the concentration of mediator in the presence of microparticle-free synovial fluid. ICAM-1, intracellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1.

    Article Snippet: IL-6, IL-8 and intracellular adhesion molecule-1 (ICAM-1; Diaclone Research, Besançon, France) and MCP-1, RANTES, VEGF and GM-CSF (BioSource International, Camarillo, CA, USA) were determined by ELISA.

    Techniques: Concentration Assay