ica 121431  (Alomone Labs)


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    Alomone Labs ica 121431
    Ica 121431, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    ica 121431  (Alomone Labs)


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    Structured Review

    Alomone Labs ica 121431
    Ica 121431, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ica 121431/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ica 121431 - by Bioz Stars, 2023-01
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    ica 121431  (Alomone Labs)


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    Alomone Labs ica 121431
    Primary cultured mouse small bowel EC cells express Scn3a and have fast voltage-gated inward currents that are selective for Na + and inhibited by the Na V 1.3 blocker <t>ICA-121431.</t> ( A ) Cropped single cell RT-PCR gel of Tph1-CFP +, Tph1-CFP- cells, or medium . ( B ) Representative traces of fast inward currents of voltage-clamped Tph1-CFP+ (EC) cells, elicited by a 2-step voltage ladder protocol ( bottom ). Inset , Peak currents of the traces shown in B , elicited during the activation ( step 1 , ●) or inactivation ( step 2 , ○) steps, plotted versus the voltage of step 1 . ( C ) Representative traces of inward currents elicited by a step from −120 to −20 mV with 150 ( black ), 100 ( blue ), or 0 mM ( cyan ) extracellular [Na + ]. Replacement of extracellular Na + with N -methyl D -glucamine (NMDG + ) diminishes (100 mM Na + ) or eliminates (0 mM Na + ) the fast inward currents. ( D ) Average peak Na + current at the −20 mV test pulse with 100 mM [Na + ] o ( blue ) or 0 mM [Na + ] o ( cyan ), normalized to the peak Na + current recorded with 150 mM [Na + ] o ( black ) (n = 4). ( E ) Representative Na + currents elicited from small bowel EC cells by steps from −120 to −20 mV with 0 ( black ) or 10 −9 to 10 −5 mol/L Na V 1.3 blocker ICA-121431 ( color gradient ). ( F ) Dose-response of peak Na + currents to ICA-121431 (IC 50 , 131 ± 54 nM; maximum block, 68 ± 7%, n = 4). Symbol (#) denotes 0.3 µM ICA-121431, the concentration used in Fig. .
    Ica 121431, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ica 121431/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ica 121431 - by Bioz Stars, 2023-01
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    1) Product Images from "Sodium channel Na V 1.3 is important for enterochromaffin cell excitability and serotonin release"

    Article Title: Sodium channel Na V 1.3 is important for enterochromaffin cell excitability and serotonin release

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-15834-3

    Primary cultured mouse small bowel EC cells express Scn3a and have fast voltage-gated inward currents that are selective for Na + and inhibited by the Na V 1.3 blocker ICA-121431. ( A ) Cropped single cell RT-PCR gel of Tph1-CFP +, Tph1-CFP- cells, or medium . ( B ) Representative traces of fast inward currents of voltage-clamped Tph1-CFP+ (EC) cells, elicited by a 2-step voltage ladder protocol ( bottom ). Inset , Peak currents of the traces shown in B , elicited during the activation ( step 1 , ●) or inactivation ( step 2 , ○) steps, plotted versus the voltage of step 1 . ( C ) Representative traces of inward currents elicited by a step from −120 to −20 mV with 150 ( black ), 100 ( blue ), or 0 mM ( cyan ) extracellular [Na + ]. Replacement of extracellular Na + with N -methyl D -glucamine (NMDG + ) diminishes (100 mM Na + ) or eliminates (0 mM Na + ) the fast inward currents. ( D ) Average peak Na + current at the −20 mV test pulse with 100 mM [Na + ] o ( blue ) or 0 mM [Na + ] o ( cyan ), normalized to the peak Na + current recorded with 150 mM [Na + ] o ( black ) (n = 4). ( E ) Representative Na + currents elicited from small bowel EC cells by steps from −120 to −20 mV with 0 ( black ) or 10 −9 to 10 −5 mol/L Na V 1.3 blocker ICA-121431 ( color gradient ). ( F ) Dose-response of peak Na + currents to ICA-121431 (IC 50 , 131 ± 54 nM; maximum block, 68 ± 7%, n = 4). Symbol (#) denotes 0.3 µM ICA-121431, the concentration used in Fig. .
    Figure Legend Snippet: Primary cultured mouse small bowel EC cells express Scn3a and have fast voltage-gated inward currents that are selective for Na + and inhibited by the Na V 1.3 blocker ICA-121431. ( A ) Cropped single cell RT-PCR gel of Tph1-CFP +, Tph1-CFP- cells, or medium . ( B ) Representative traces of fast inward currents of voltage-clamped Tph1-CFP+ (EC) cells, elicited by a 2-step voltage ladder protocol ( bottom ). Inset , Peak currents of the traces shown in B , elicited during the activation ( step 1 , ●) or inactivation ( step 2 , ○) steps, plotted versus the voltage of step 1 . ( C ) Representative traces of inward currents elicited by a step from −120 to −20 mV with 150 ( black ), 100 ( blue ), or 0 mM ( cyan ) extracellular [Na + ]. Replacement of extracellular Na + with N -methyl D -glucamine (NMDG + ) diminishes (100 mM Na + ) or eliminates (0 mM Na + ) the fast inward currents. ( D ) Average peak Na + current at the −20 mV test pulse with 100 mM [Na + ] o ( blue ) or 0 mM [Na + ] o ( cyan ), normalized to the peak Na + current recorded with 150 mM [Na + ] o ( black ) (n = 4). ( E ) Representative Na + currents elicited from small bowel EC cells by steps from −120 to −20 mV with 0 ( black ) or 10 −9 to 10 −5 mol/L Na V 1.3 blocker ICA-121431 ( color gradient ). ( F ) Dose-response of peak Na + currents to ICA-121431 (IC 50 , 131 ± 54 nM; maximum block, 68 ± 7%, n = 4). Symbol (#) denotes 0.3 µM ICA-121431, the concentration used in Fig. .

    Techniques Used: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Activation Assay, Blocking Assay, Concentration Assay

    Na V 1.3 is necessary for EC cell action potentials. ( A , B ) Typical action potentials from small bowel ( A ) and colon ( B ) EC cells, elicited by 50-ms depolarizing current steps ( bottom ). ( C , D ) Elicited EC cell action potentials were blocked by Na + ion replacement ( C ) or Na V 1.3 inhibition using ICA-121431 (300 nM, as highlighted by # in Fig. ). ( D ) Insets , first derivatives of the traces in each panel, showing the rate of change in membrane potential induced by the current injection protocol. The maximum depolarization rate is an approximate measure of the inward current through voltage-gated sodium channels.
    Figure Legend Snippet: Na V 1.3 is necessary for EC cell action potentials. ( A , B ) Typical action potentials from small bowel ( A ) and colon ( B ) EC cells, elicited by 50-ms depolarizing current steps ( bottom ). ( C , D ) Elicited EC cell action potentials were blocked by Na + ion replacement ( C ) or Na V 1.3 inhibition using ICA-121431 (300 nM, as highlighted by # in Fig. ). ( D ) Insets , first derivatives of the traces in each panel, showing the rate of change in membrane potential induced by the current injection protocol. The maximum depolarization rate is an approximate measure of the inward current through voltage-gated sodium channels.

    Techniques Used: Inhibition, Injection

    Na V 1.3 contributes toward 5-HT release from EC cells. The change in release of 5-HT (Δ5-HT) from primary colonic EC cells was measured by ELISA following 20 min incubation with KCl ( left ),BDS-1 ( middle ) or butyrate ( right ), in the absence ( black ) or presence ( gray ) of Na V 1.3 inhibitor ICA-121431.
    Figure Legend Snippet: Na V 1.3 contributes toward 5-HT release from EC cells. The change in release of 5-HT (Δ5-HT) from primary colonic EC cells was measured by ELISA following 20 min incubation with KCl ( left ),BDS-1 ( middle ) or butyrate ( right ), in the absence ( black ) or presence ( gray ) of Na V 1.3 inhibitor ICA-121431.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Incubation

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    Alomone Labs ica 121431
    Ica 121431, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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