iκb kinase β ikkβ inhibitor peptide  (Millipore)


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    Custom Peptides
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    PEPscreen Custom Peptide LIbraries Suitable for robotic production of peptide microarrays Screen mulitple proteotyptic peptides and rapidly characterize and select mass spec compatible peptides Get results quick with delivery of your peptide library in less than 7 business days Sequence Length 6 to 20 amino acids Amount 0 5 2 mg or 2 5 mg Modifications Comprehensive offering including phosphorylation biotin fluorescein stable isotopes etc Format Supplied dry in a 96 well tube rack Options Normalization and aliquoting stock and copy libraries Minimum library size of 24 peptidesRequest more informationUse our free design tool to design your libraryAQUA Peptides Accurately quantitate low abundance proteins Measure site specific phosphorylation states Validate gene silencing at the protein level Custom peptides are available either without stable isotope labeling or with stable isotope labeling Stable isotope labeled AQUA TM Peptide seqeunces contain one isotopically labeled amino acid Sequence Length 5 to 30 amino acids Amount 5 x 1 nmole Purity 95 by RP HPLC Modifications phosphorylation Ser Thr Tyr carboxymethylated Cys carbamidomethylated Cys hydroxyproline N terminal biotin please inquire regarding other modifications Quantification by amino acid analysis AAA Format Supplied dryRequest more information
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    Millipore iκb kinase β ikkβ inhibitor peptide
    Custom Peptides
    PEPscreen Custom Peptide LIbraries Suitable for robotic production of peptide microarrays Screen mulitple proteotyptic peptides and rapidly characterize and select mass spec compatible peptides Get results quick with delivery of your peptide library in less than 7 business days Sequence Length 6 to 20 amino acids Amount 0 5 2 mg or 2 5 mg Modifications Comprehensive offering including phosphorylation biotin fluorescein stable isotopes etc Format Supplied dry in a 96 well tube rack Options Normalization and aliquoting stock and copy libraries Minimum library size of 24 peptidesRequest more informationUse our free design tool to design your libraryAQUA Peptides Accurately quantitate low abundance proteins Measure site specific phosphorylation states Validate gene silencing at the protein level Custom peptides are available either without stable isotope labeling or with stable isotope labeling Stable isotope labeled AQUA TM Peptide seqeunces contain one isotopically labeled amino acid Sequence Length 5 to 30 amino acids Amount 5 x 1 nmole Purity 95 by RP HPLC Modifications phosphorylation Ser Thr Tyr carboxymethylated Cys carbamidomethylated Cys hydroxyproline N terminal biotin please inquire regarding other modifications Quantification by amino acid analysis AAA Format Supplied dryRequest more information
    https://www.bioz.com/result/iκb kinase β ikkβ inhibitor peptide/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iκb kinase β ikkβ inhibitor peptide - by Bioz Stars, 2020-08
    99/100 stars

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    High Performance Liquid Chromatography:

    Article Title: An Additional Function of the Rough Endoplasmic Reticulum Protein Complex Prolyl 3-Hydroxylase 1·Cartilage-associated Protein·Cyclophilin B
    Article Snippet: .. The thiol-disulfide interchange equilibria were established between the reduced GCRALCG peptide and β-hydroxyethyl disulfide (Sigma-Aldrich) or the oxidized GCRALCG peptide and β - mercaptoethanol (Sigma-Aldrich) in various ratios at 25 °C for 24 h. After the reaction was quenched by trifluoroacetic acid, an aliquot of the mixture was loaded onto a Vydac analytical C4 reverse phase column using a HPLC system, and the separated peaks were detected by absorbance at 205 nm. ..

    Article Title: Common fibrillar spines of amyloid-β and human islet amyloid polypeptide revealed by microelectron diffraction and structure-based inhibitors
    Article Snippet: .. Pooled recombinant Aβ peptide was filtered through a 0.22-μm filter unit (Steriflip, Millipore) and further purified by reverse-phase high-performance liquid chromatography (HPLC) on a 21.2 × 250-mm Agilent 897250–106 Zorbax StableBond 300 C8 PrepHT cartridge with 7-μm beads at 80 °C equilibrated in buffer RA (0.1% trifluoroacetic acid (TFA), water) and eluted over a linear gradient from 15 to 50% buffer RB (acetonitrile, 0.1% TFA) in 59 min at a flow rate of 10 ml/min. .. Absorbances at 220 and 280 nm were recorded using a Waters 2487 dual λ absorbance detector.

    Flow Cytometry:

    Article Title: Common fibrillar spines of amyloid-β and human islet amyloid polypeptide revealed by microelectron diffraction and structure-based inhibitors
    Article Snippet: .. Pooled recombinant Aβ peptide was filtered through a 0.22-μm filter unit (Steriflip, Millipore) and further purified by reverse-phase high-performance liquid chromatography (HPLC) on a 21.2 × 250-mm Agilent 897250–106 Zorbax StableBond 300 C8 PrepHT cartridge with 7-μm beads at 80 °C equilibrated in buffer RA (0.1% trifluoroacetic acid (TFA), water) and eluted over a linear gradient from 15 to 50% buffer RB (acetonitrile, 0.1% TFA) in 59 min at a flow rate of 10 ml/min. .. Absorbances at 220 and 280 nm were recorded using a Waters 2487 dual λ absorbance detector.

    Positron Emission Tomography:

    Article Title: Identification of Ly2 members as antimicrobial peptides from zebrafish Danio rerio
    Article Snippet: .. To express the TRX-His-tag peptide as control, DE3 cells were also transformed by plasmid pET-32a(+) (Novagen) and induced with IPTG at a final concentration of 0.5 mM at 28°C for 8 h. The peptide was purified and proceeded as described above. .. Refolding of rLy2.1-3 The purified rLy2.1-3 were dialysed successively in the dialysis buffer I (25 mM Tris, 200 mM NaCl, 1 mM EDTA, 5 M urea and 5 mM DTT, pH 6.5) for 6 h; dialysis buffer II (25 mM Tris, 50 mM NaCl, 1 mM EDTA, 4 M urea, 0.2 mM GSSG and 2 mM GSH, pH 6.5) for 6 h; dialysis buffer III (25 mM Tris, 20 mM NaCl, 1 mM EDTA, 2 M urea, 0.5 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer IV (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 1 M urea, 5% glycerol, 5 mM DTT, 1 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer V (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 0.5 M urea, 5% (v/v) glycerol, 1% (w/v) glycine, 5 mM DTT, 1 mM GSSG, 1 mM GSH, pH 6.5) for 6 h; dialysis buffer VI (25 mM Tris, 10 mM NaCl, 5% glycerol, 1% (w/v) glycine and 5 mM DTT, pH 6.5) for 6 h; and dialysis buffer VII (25 mM Tris and 10 mM NaCl, pH 6.5) for 6 h. The protein concentrations were determined by the method of BCA using BSA as a standard.

    Purification:

    Article Title: Identification of Ly2 members as antimicrobial peptides from zebrafish Danio rerio
    Article Snippet: .. To express the TRX-His-tag peptide as control, DE3 cells were also transformed by plasmid pET-32a(+) (Novagen) and induced with IPTG at a final concentration of 0.5 mM at 28°C for 8 h. The peptide was purified and proceeded as described above. .. Refolding of rLy2.1-3 The purified rLy2.1-3 were dialysed successively in the dialysis buffer I (25 mM Tris, 200 mM NaCl, 1 mM EDTA, 5 M urea and 5 mM DTT, pH 6.5) for 6 h; dialysis buffer II (25 mM Tris, 50 mM NaCl, 1 mM EDTA, 4 M urea, 0.2 mM GSSG and 2 mM GSH, pH 6.5) for 6 h; dialysis buffer III (25 mM Tris, 20 mM NaCl, 1 mM EDTA, 2 M urea, 0.5 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer IV (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 1 M urea, 5% glycerol, 5 mM DTT, 1 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer V (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 0.5 M urea, 5% (v/v) glycerol, 1% (w/v) glycine, 5 mM DTT, 1 mM GSSG, 1 mM GSH, pH 6.5) for 6 h; dialysis buffer VI (25 mM Tris, 10 mM NaCl, 5% glycerol, 1% (w/v) glycine and 5 mM DTT, pH 6.5) for 6 h; and dialysis buffer VII (25 mM Tris and 10 mM NaCl, pH 6.5) for 6 h. The protein concentrations were determined by the method of BCA using BSA as a standard.

    Article Title: Common fibrillar spines of amyloid-β and human islet amyloid polypeptide revealed by microelectron diffraction and structure-based inhibitors
    Article Snippet: .. Pooled recombinant Aβ peptide was filtered through a 0.22-μm filter unit (Steriflip, Millipore) and further purified by reverse-phase high-performance liquid chromatography (HPLC) on a 21.2 × 250-mm Agilent 897250–106 Zorbax StableBond 300 C8 PrepHT cartridge with 7-μm beads at 80 °C equilibrated in buffer RA (0.1% trifluoroacetic acid (TFA), water) and eluted over a linear gradient from 15 to 50% buffer RB (acetonitrile, 0.1% TFA) in 59 min at a flow rate of 10 ml/min. .. Absorbances at 220 and 280 nm were recorded using a Waters 2487 dual λ absorbance detector.

    Enzyme-linked Immunosorbent Assay:

    Article Title: LP-925219 maximizes urinary glucose excretion in mice by inhibiting both renal SGLT1 and SGLT2
    Article Snippet: .. The blood obtained at 60 min was also used to measure circulating levels of total GLP-1 (tGLP-1; Glucagon-Like Peptide-1 Total ELISA Kit, catalog #EZGLP1T-36K, EMD Millipore, Billerica, MA) as described previously (Powell et al. ). .. After their blood was drawn at 60 min, mice were anesthetized with isoflurane (TW Medical Veterinary Supply, Lago Vista, TX) and then euthanized by cervical dislocation.

    Concentration Assay:

    Article Title: Identification of Ly2 members as antimicrobial peptides from zebrafish Danio rerio
    Article Snippet: .. To express the TRX-His-tag peptide as control, DE3 cells were also transformed by plasmid pET-32a(+) (Novagen) and induced with IPTG at a final concentration of 0.5 mM at 28°C for 8 h. The peptide was purified and proceeded as described above. .. Refolding of rLy2.1-3 The purified rLy2.1-3 were dialysed successively in the dialysis buffer I (25 mM Tris, 200 mM NaCl, 1 mM EDTA, 5 M urea and 5 mM DTT, pH 6.5) for 6 h; dialysis buffer II (25 mM Tris, 50 mM NaCl, 1 mM EDTA, 4 M urea, 0.2 mM GSSG and 2 mM GSH, pH 6.5) for 6 h; dialysis buffer III (25 mM Tris, 20 mM NaCl, 1 mM EDTA, 2 M urea, 0.5 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer IV (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 1 M urea, 5% glycerol, 5 mM DTT, 1 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer V (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 0.5 M urea, 5% (v/v) glycerol, 1% (w/v) glycine, 5 mM DTT, 1 mM GSSG, 1 mM GSH, pH 6.5) for 6 h; dialysis buffer VI (25 mM Tris, 10 mM NaCl, 5% glycerol, 1% (w/v) glycine and 5 mM DTT, pH 6.5) for 6 h; and dialysis buffer VII (25 mM Tris and 10 mM NaCl, pH 6.5) for 6 h. The protein concentrations were determined by the method of BCA using BSA as a standard.

    Article Title: A Novel Phage-Library-Selected Peptide Inhibits Human TNF-α Binding to Its Receptors
    Article Snippet: .. Samples were fixed through incubation with a PBS-4% paraformaldehyde (PFA) solution for 15 min, saturated for 30 min at 37 °C with PBS-1% BSA and incubated with 500 nM biotinylated hTNF-α and different concentration (20, 10 and 1 µM) of anti-TNF-α peptide for 30 min at room temperature, then with 0.5 μg/mL Streptavidin-Atto 550 (Sigma-Aldrich) for 15 min at room temperature. .. Nuclei were stained with DAPI. hTNF-α binding to receptors was analyzed by confocal laser microscope (Leica TCS SP5) with 364/555 nm excitation and 458/570 nm emission filters for DAPI and Atto 550, respectively.

    Incubation:

    Article Title: The Obesity-Related Peptide Leptin Sensitizes Cardiac Mitochondria to Calcium-Induced Permeability Transition Pore Opening and Apoptosis
    Article Snippet: .. Cardiomyocytes were treated with vehicle (control) or 3.1 nM leptin (50 ng/ml, Sigma-Aldrich, Oakville, Ontario, Canada) for 24 hours after 30 minutes of incubation in the absence or presence of the STAT3 inhibitor peptide (SIP, 1 mM, Calbiochem, San Diego, CA, USA); the mPTP inhibitor Sanglifehrin A (0.2 µM, gift of Novartis Pharmaceuticals, Basel, Switzerland) or the ROCK inhibitor Y27632 (10 µM, Sigma-Aldrich). .. For some experiments we also determined the effect of the Stat1 inhibitor fludarabine (9-β-D-arabinofuranosyl-2-fluoroadenine 5′-phosphate, 50 µM, Sigma-Aldrich) which was also added 30 minutes before leptin administration.

    Article Title: A Novel Phage-Library-Selected Peptide Inhibits Human TNF-α Binding to Its Receptors
    Article Snippet: .. Samples were fixed through incubation with a PBS-4% paraformaldehyde (PFA) solution for 15 min, saturated for 30 min at 37 °C with PBS-1% BSA and incubated with 500 nM biotinylated hTNF-α and different concentration (20, 10 and 1 µM) of anti-TNF-α peptide for 30 min at room temperature, then with 0.5 μg/mL Streptavidin-Atto 550 (Sigma-Aldrich) for 15 min at room temperature. .. Nuclei were stained with DAPI. hTNF-α binding to receptors was analyzed by confocal laser microscope (Leica TCS SP5) with 364/555 nm excitation and 458/570 nm emission filters for DAPI and Atto 550, respectively.

    Transformation Assay:

    Article Title: Identification of Ly2 members as antimicrobial peptides from zebrafish Danio rerio
    Article Snippet: .. To express the TRX-His-tag peptide as control, DE3 cells were also transformed by plasmid pET-32a(+) (Novagen) and induced with IPTG at a final concentration of 0.5 mM at 28°C for 8 h. The peptide was purified and proceeded as described above. .. Refolding of rLy2.1-3 The purified rLy2.1-3 were dialysed successively in the dialysis buffer I (25 mM Tris, 200 mM NaCl, 1 mM EDTA, 5 M urea and 5 mM DTT, pH 6.5) for 6 h; dialysis buffer II (25 mM Tris, 50 mM NaCl, 1 mM EDTA, 4 M urea, 0.2 mM GSSG and 2 mM GSH, pH 6.5) for 6 h; dialysis buffer III (25 mM Tris, 20 mM NaCl, 1 mM EDTA, 2 M urea, 0.5 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer IV (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 1 M urea, 5% glycerol, 5 mM DTT, 1 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer V (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 0.5 M urea, 5% (v/v) glycerol, 1% (w/v) glycine, 5 mM DTT, 1 mM GSSG, 1 mM GSH, pH 6.5) for 6 h; dialysis buffer VI (25 mM Tris, 10 mM NaCl, 5% glycerol, 1% (w/v) glycine and 5 mM DTT, pH 6.5) for 6 h; and dialysis buffer VII (25 mM Tris and 10 mM NaCl, pH 6.5) for 6 h. The protein concentrations were determined by the method of BCA using BSA as a standard.

    Recombinant:

    Article Title: Common fibrillar spines of amyloid-β and human islet amyloid polypeptide revealed by microelectron diffraction and structure-based inhibitors
    Article Snippet: .. Pooled recombinant Aβ peptide was filtered through a 0.22-μm filter unit (Steriflip, Millipore) and further purified by reverse-phase high-performance liquid chromatography (HPLC) on a 21.2 × 250-mm Agilent 897250–106 Zorbax StableBond 300 C8 PrepHT cartridge with 7-μm beads at 80 °C equilibrated in buffer RA (0.1% trifluoroacetic acid (TFA), water) and eluted over a linear gradient from 15 to 50% buffer RB (acetonitrile, 0.1% TFA) in 59 min at a flow rate of 10 ml/min. .. Absorbances at 220 and 280 nm were recorded using a Waters 2487 dual λ absorbance detector.

    Plasmid Preparation:

    Article Title: Identification of Ly2 members as antimicrobial peptides from zebrafish Danio rerio
    Article Snippet: .. To express the TRX-His-tag peptide as control, DE3 cells were also transformed by plasmid pET-32a(+) (Novagen) and induced with IPTG at a final concentration of 0.5 mM at 28°C for 8 h. The peptide was purified and proceeded as described above. .. Refolding of rLy2.1-3 The purified rLy2.1-3 were dialysed successively in the dialysis buffer I (25 mM Tris, 200 mM NaCl, 1 mM EDTA, 5 M urea and 5 mM DTT, pH 6.5) for 6 h; dialysis buffer II (25 mM Tris, 50 mM NaCl, 1 mM EDTA, 4 M urea, 0.2 mM GSSG and 2 mM GSH, pH 6.5) for 6 h; dialysis buffer III (25 mM Tris, 20 mM NaCl, 1 mM EDTA, 2 M urea, 0.5 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer IV (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 1 M urea, 5% glycerol, 5 mM DTT, 1 mM GSSG and 1 mM GSH, pH 6.5) for 6 h; dialysis buffer V (25 mM Tris, 10 mM NaCl, 1 mM EDTA, 0.5 M urea, 5% (v/v) glycerol, 1% (w/v) glycine, 5 mM DTT, 1 mM GSSG, 1 mM GSH, pH 6.5) for 6 h; dialysis buffer VI (25 mM Tris, 10 mM NaCl, 5% glycerol, 1% (w/v) glycine and 5 mM DTT, pH 6.5) for 6 h; and dialysis buffer VII (25 mM Tris and 10 mM NaCl, pH 6.5) for 6 h. The protein concentrations were determined by the method of BCA using BSA as a standard.

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  • 99
    Millipore iκb kinase β ikkβ inhibitor peptide
    Iκb Kinase β Ikkβ Inhibitor Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iκb kinase β ikkβ inhibitor peptide/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iκb kinase β ikkβ inhibitor peptide - by Bioz Stars, 2020-08
    99/100 stars
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