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Ventana Medical i view dab detection kit
I View Dab Detection Kit, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 95/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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i view dab detection kit - by Bioz Stars, 2020-02
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Immunohistochemistry:

Article Title: A prospective study of mediastinal gray-zone lymphoma
Article Snippet: Paragraph title: Immunohistochemical analysis ... I-View DAB Detection Kit (Ventana Medical Systems, Tucson, AZ) was used as a detection system.

Article Title: Plasmalemmal Vesicle Associated Protein (PLVAP) as a therapeutic target for treatment of hepatocellular carcinoma
Article Snippet: Paragraph title: Immunohistochemical staining for PLVAP expression ... The sections were then processed using the i View™ DAB Detection Kit (Ventana Medical Systems).

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana).

Article Title: Progranulin as a Prognostic Biomarker for Breast Cancer Recurrence in Patients Who Had Hormone Receptor-Positive Tumors: A Cohort Study
Article Snippet: Paragraph title: Immunohistochemical Staining ... Staining was performed using the I-View DAB detection kit and a Ventana ES autostainer (Ventana Medical Systems, Tucson, AZ, USA) using primary antibodies against ER and PR (both from Ventana Medical Systems).

Article Title: Low expression of MUC2 is associated with longer disease-free survival in patients with colorectal carcinoma
Article Snippet: Paragraph title: Immunohistochemistry ... Pretreatment was done using CC1 (prediluted cell conditioning solution) for 60 min. Antihuman mouse anti-MUC2 polyclonal antibody (Cell Marque; MRQ-18) was incubated at 37°C for 20 min. Ventana® I-view DAB detection kit was used according to kit manufacturer instructions.

Article Title: Hemodynamic Response Imaging: A Potential Tool for the Assessment of Angiogenesis in Brain Tumors
Article Snippet: .. Immunohistochemical staining was performed on the Benchmark XT autostainer (Ventana Medical Systems Inc.) using the I-View DAB detection kit (Ventana). .. Statistical analysis Statistical analysis was performed on data obtained from the healthy subjects (n = 19) using SPSS 12.0 (SPSS Inc., Chicago, IL, USA).

Article Title: Immunohistochemical Analysis of Oral Dysplasia: Diagnostic Assessment by Fascin and Podoplanin Expression
Article Snippet: Paragraph title: Immunohistochemical procedure ... The slides were incubated with specific primary antibodies raised against fascin (mouse monoclonal antibody, clone 55K-2; DAKO) at a 1:100 dilution and podoplanin (mouse monoclonal antibody, clone NZ-1; AngioBio, Del Mar, CA) at a 1:200 dilution at 37°C for 32 min. Immunostaining with an i VIEW DAB Detection Kit (Ventana Medical Systems Inc., AZ) and BenchMark XT (Ventana Medical Systems) was performed.

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Five-micrometer sections from formalin-perfused SpT ( n = 2), SA, FT, and interbout arousal (IBA) (all n = 3) were deparaffinized, then processed for immunohistochemical detection of Ki-67. .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Article Title: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections
Article Snippet: .. IHC determination of HER2 protein expression The FDA-approved HER2 IHC assay using PATHWAY HER-2/neu rabbit monoclonal antibody (clone 4B5; Ventana) was performed with i View DAB Detection Kit (Ventana) on a BenchMark XT automated staining system (Ventana). .. Briefly, the tissue sections were deparaffinized with EZ Prep (Ventana) at 75°C, heat pretreated in Cell Conditioning 1 (CC1; Ventana) using “standard cell conditioning” for antigen retrieval at 100°C, and then incubated with the anti-HER2 primary antibody for 32 min at 37°C after inactivation of the endogenous peroxidase with hydrogen peroxide for 4 min.

Article Title: The prognostic significance of tumor lymphangiogenesis and lymphatic vessel density in endometrioid carcinoma of the uterine corpus
Article Snippet: .. Immunohistochemistry was conducted using a BenchMark XT (Ventana Medical Systems Inc., Tucson, AZ, USA) and an I-View DAB detection kit (Ventana Medical Systems Inc.) at a dilution of 1:100 for both antibodies. ..

Article Title: Biological importance of podoplanin expression in chorionic villous stromal cells and its relationship to placental pathologies
Article Snippet: .. Immunohistochemical staining and evaluation Immunohistochemical staining was performed on a fully automatic immunostaining device (BenchMark XT; Ventana Medical Systems Inc., Tucson, AZ, USA) using podoplanin (Clone D2-40, diluted 1:100) and an I-View DAB detection kit (Ventana Medical Systems Inc.). .. Internal (endometrial blood and lymphatic vascular endothelium) and external (skin-dermal blood and lymphatic vascular endothelium) negative/positive control tissues were included in each staining.

Article Title: Desmoglein 1 deficiency results in severe dermatitis, multiple allergies and metabolic wasting
Article Snippet: Paragraph title: Immunohistochemistry ... Visualization of the bound primary antibodies was performed using the I-View DAB detection kit (Ventana Medical System).

Article Title: A Crucial Role for Kupffer Cell-Derived Galectin-9 in Regulation of T Cell Immunity in Hepatitis C Infection
Article Snippet: Paragraph title: Galectin-9 Immunohistochemistry ... An I-VIEW DAB detection kit (Ventana) was used for antibody detection and included endogenous peroxidase block, secondary antibody, streptavidin horseradish peroxidase, DAB substrate with hydrogen peroxide activator and copper enhancer.

Article Title: Major Functional Transcriptome of an Inferred Center Regulator of an ER(-) Breast Cancer Model System
Article Snippet: Paragraph title: Immunohistochemical (IHC) stain of paraffin-embedded breast cancer slides ... For visualization of immunostain, we applied diaminobenzidine as a chromogen (i view™ DAB Detection Kit, Ventana Medical Systems, USA).

Sample Prep:

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Paragraph title: Histology sample preparation and analysis. ... Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Single-particle Tracking:

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Five-micrometer sections from formalin-perfused SpT ( n = 2), SA, FT, and interbout arousal (IBA) (all n = 3) were deparaffinized, then processed for immunohistochemical detection of Ki-67. .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Mouse Assay:

Article Title: Podocytes Contribute to the Formation of Glomerular Crescents
Article Snippet: Mice were examined at 5.5 wk of age; 5-μm formalin-fixed, paraffin-embedded tissue sections were mounted on positively charged microscope slides and then baked overnight at 60°C before immunostaining. .. The detection system used a rabbit biotinylated secondary antibody (Vector) with the Ventana i-VIEW DAB detection kit.

Immunodetection:

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: Immunodetection of Ki-67, cleaved caspase-3 and erbB2 was performed on the NexES stainer (Ventana) at an operating temperature of 37°C. .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana).

Microscopy:

Article Title: Podocytes Contribute to the Formation of Glomerular Crescents
Article Snippet: Mice were examined at 5.5 wk of age; 5-μm formalin-fixed, paraffin-embedded tissue sections were mounted on positively charged microscope slides and then baked overnight at 60°C before immunostaining. .. The detection system used a rabbit biotinylated secondary antibody (Vector) with the Ventana i-VIEW DAB detection kit.

Avidin-Biotin Assay:

Article Title: Immunohistochemical Analysis of Oral Dysplasia: Diagnostic Assessment by Fascin and Podoplanin Expression
Article Snippet: Immunohistochemical procedure Immunohistochemistry was performed using the avidin-biotin peroxidase complex technique. .. The slides were incubated with specific primary antibodies raised against fascin (mouse monoclonal antibody, clone 55K-2; DAKO) at a 1:100 dilution and podoplanin (mouse monoclonal antibody, clone NZ-1; AngioBio, Del Mar, CA) at a 1:200 dilution at 37°C for 32 min. Immunostaining with an i VIEW DAB Detection Kit (Ventana Medical Systems Inc., AZ) and BenchMark XT (Ventana Medical Systems) was performed.

IA:

Article Title: Plasmalemmal Vesicle Associated Protein (PLVAP) as a therapeutic target for treatment of hepatocellular carcinoma
Article Snippet: This hybridoma was obtained from the Developmental Studies Hybridoma Bank at the University of Iowa (Iowa City, IA). .. The sections were then processed using the i View™ DAB Detection Kit (Ventana Medical Systems).

Incubation:

Article Title: Plasmalemmal Vesicle Associated Protein (PLVAP) as a therapeutic target for treatment of hepatocellular carcinoma
Article Snippet: After antigen retrieval and blocking of endogenous peroxidase, tissue sections were incubated with 1 μg/ml anti-human or 5 μg/ml anti-mouse PLVAP monoclonal antibody at 37°C for 48 minutes. .. The sections were then processed using the i View™ DAB Detection Kit (Ventana Medical Systems).

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana). .. The I-VIEW secondary antibody and enzyme were replaced with a species-specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 minutes) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA, USA; 8 minutes).

Article Title: Low expression of MUC2 is associated with longer disease-free survival in patients with colorectal carcinoma
Article Snippet: .. Pretreatment was done using CC1 (prediluted cell conditioning solution) for 60 min. Antihuman mouse anti-MUC2 polyclonal antibody (Cell Marque; MRQ-18) was incubated at 37°C for 20 min. Ventana® I-view DAB detection kit was used according to kit manufacturer instructions. ..

Article Title: Immunohistochemical Analysis of Oral Dysplasia: Diagnostic Assessment by Fascin and Podoplanin Expression
Article Snippet: .. The slides were incubated with specific primary antibodies raised against fascin (mouse monoclonal antibody, clone 55K-2; DAKO) at a 1:100 dilution and podoplanin (mouse monoclonal antibody, clone NZ-1; AngioBio, Del Mar, CA) at a 1:200 dilution at 37°C for 32 min. Immunostaining with an i VIEW DAB Detection Kit (Ventana Medical Systems Inc., AZ) and BenchMark XT (Ventana Medical Systems) was performed. ..

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ). .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

Article Title: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections
Article Snippet: IHC determination of HER2 protein expression The FDA-approved HER2 IHC assay using PATHWAY HER-2/neu rabbit monoclonal antibody (clone 4B5; Ventana) was performed with i View DAB Detection Kit (Ventana) on a BenchMark XT automated staining system (Ventana). .. Briefly, the tissue sections were deparaffinized with EZ Prep (Ventana) at 75°C, heat pretreated in Cell Conditioning 1 (CC1; Ventana) using “standard cell conditioning” for antigen retrieval at 100°C, and then incubated with the anti-HER2 primary antibody for 32 min at 37°C after inactivation of the endogenous peroxidase with hydrogen peroxide for 4 min.

Article Title: A Crucial Role for Kupffer Cell-Derived Galectin-9 in Regulation of T Cell Immunity in Hepatitis C Infection
Article Snippet: An I-VIEW DAB detection kit (Ventana) was used for antibody detection and included endogenous peroxidase block, secondary antibody, streptavidin horseradish peroxidase, DAB substrate with hydrogen peroxide activator and copper enhancer. .. Following the 16 minute primary antibody incubation, the secondary and SA-HRP antibodies were applied for 8 minutes respectively.

Formalin-fixed Paraffin-Embedded:

Article Title: A prospective study of mediastinal gray-zone lymphoma
Article Snippet: Immunohistochemistry (IHC) was performed on formalin-fixed, paraffin-embedded tissue sections and included CD20, CD3, CD79a, PAX-5, OCT-2, Bob-1, BCL-6, CD68, and dendritic cell–specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN). .. I-View DAB Detection Kit (Ventana Medical Systems, Tucson, AZ) was used as a detection system.

Article Title: Progranulin as a Prognostic Biomarker for Breast Cancer Recurrence in Patients Who Had Hormone Receptor-Positive Tumors: A Cohort Study
Article Snippet: Immunohistochemical Staining To assess the ER and PR expression status, immunohistochemical staining was performed using tissue sections cut from formalin-fixed, paraffin-embedded representative breast tumors. .. Staining was performed using the I-View DAB detection kit and a Ventana ES autostainer (Ventana Medical Systems, Tucson, AZ, USA) using primary antibodies against ER and PR (both from Ventana Medical Systems).

Article Title: Podocytes Contribute to the Formation of Glomerular Crescents
Article Snippet: Mice were examined at 5.5 wk of age; 5-μm formalin-fixed, paraffin-embedded tissue sections were mounted on positively charged microscope slides and then baked overnight at 60°C before immunostaining. .. The detection system used a rabbit biotinylated secondary antibody (Vector) with the Ventana i-VIEW DAB detection kit.

Blocking Assay:

Article Title: Plasmalemmal Vesicle Associated Protein (PLVAP) as a therapeutic target for treatment of hepatocellular carcinoma
Article Snippet: After antigen retrieval and blocking of endogenous peroxidase, tissue sections were incubated with 1 μg/ml anti-human or 5 μg/ml anti-mouse PLVAP monoclonal antibody at 37°C for 48 minutes. .. The sections were then processed using the i View™ DAB Detection Kit (Ventana Medical Systems).

Article Title: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections
Article Snippet: IHC determination of HER2 protein expression The FDA-approved HER2 IHC assay using PATHWAY HER-2/neu rabbit monoclonal antibody (clone 4B5; Ventana) was performed with i View DAB Detection Kit (Ventana) on a BenchMark XT automated staining system (Ventana). .. They were then blocked using Endogenous Biotin Blocking Kit (Ventana), incubated with a biotinylated secondary antibody for 8 min, and incubated with a streptavidin-HRP conjugate for 8 min at 37°C.

Article Title: A Crucial Role for Kupffer Cell-Derived Galectin-9 in Regulation of T Cell Immunity in Hepatitis C Infection
Article Snippet: .. An I-VIEW DAB detection kit (Ventana) was used for antibody detection and included endogenous peroxidase block, secondary antibody, streptavidin horseradish peroxidase, DAB substrate with hydrogen peroxide activator and copper enhancer. .. A biotinylated rabbit anti-goat secondary antibody (Jackson ImmunoResearch; West Grove, PA) used at a 1∶50 dilution in PBS, pH 7.6 was used in place of the secondary antibody included in the I-View kit.

Expressing:

Article Title: Plasmalemmal Vesicle Associated Protein (PLVAP) as a therapeutic target for treatment of hepatocellular carcinoma
Article Snippet: Paragraph title: Immunohistochemical staining for PLVAP expression ... The sections were then processed using the i View™ DAB Detection Kit (Ventana Medical Systems).

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: For the negative controls, in addition to use the same cells/tissues without addition of the primary antibodies, we also performed the IHC assays using the tissues or cell lines that are known to have no expression of the antigens (Additional file ). .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana).

Article Title: Progranulin as a Prognostic Biomarker for Breast Cancer Recurrence in Patients Who Had Hormone Receptor-Positive Tumors: A Cohort Study
Article Snippet: Immunohistochemical Staining To assess the ER and PR expression status, immunohistochemical staining was performed using tissue sections cut from formalin-fixed, paraffin-embedded representative breast tumors. .. Staining was performed using the I-View DAB detection kit and a Ventana ES autostainer (Ventana Medical Systems, Tucson, AZ, USA) using primary antibodies against ER and PR (both from Ventana Medical Systems).

Article Title: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections
Article Snippet: .. IHC determination of HER2 protein expression The FDA-approved HER2 IHC assay using PATHWAY HER-2/neu rabbit monoclonal antibody (clone 4B5; Ventana) was performed with i View DAB Detection Kit (Ventana) on a BenchMark XT automated staining system (Ventana). .. Briefly, the tissue sections were deparaffinized with EZ Prep (Ventana) at 75°C, heat pretreated in Cell Conditioning 1 (CC1; Ventana) using “standard cell conditioning” for antigen retrieval at 100°C, and then incubated with the anti-HER2 primary antibody for 32 min at 37°C after inactivation of the endogenous peroxidase with hydrogen peroxide for 4 min.

Article Title: Biological importance of podoplanin expression in chorionic villous stromal cells and its relationship to placental pathologies
Article Snippet: Immunohistochemical staining and evaluation Immunohistochemical staining was performed on a fully automatic immunostaining device (BenchMark XT; Ventana Medical Systems Inc., Tucson, AZ, USA) using podoplanin (Clone D2-40, diluted 1:100) and an I-View DAB detection kit (Ventana Medical Systems Inc.). .. Villous and extravillous trophoblastic cells, Hofbauer cells, decidua, fetal membranes, endometrial glandular epithelium, and endometrial stromal cells were also examined for podoplanin expression, but were not scored.

Modification:

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana). .. The I-VIEW secondary antibody and enzyme were replaced with a species-specific secondary antibody (biotinylated goat anti-rabbit; 1:75; cat# 111-065-144; Jackson ImmunoResearch; 8 minutes) and streptavidin-horseradish (SA-HRP; 1:50; cat# SA-5004; DAKO Cytomation, Carpinteria, CA, USA; 8 minutes).

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA). ..

Article Title: Podocytes Contribute to the Formation of Glomerular Crescents
Article Snippet: The detection system used a rabbit biotinylated secondary antibody (Vector) with the Ventana i-VIEW DAB detection kit. .. To avoid mouse-on-mouse cross reaction, a modification of the ARK (Dako) detection procedure was adapted for use on the immunostainer.

Staining:

Article Title: A prospective study of mediastinal gray-zone lymphoma
Article Snippet: I-View DAB Detection Kit (Ventana Medical Systems, Tucson, AZ) was used as a detection system. .. Positive controls were run with each set of slides and showed appropriate staining patterns.

Article Title: Plasmalemmal Vesicle Associated Protein (PLVAP) as a therapeutic target for treatment of hepatocellular carcinoma
Article Snippet: Paragraph title: Immunohistochemical staining for PLVAP expression ... The sections were then processed using the i View™ DAB Detection Kit (Ventana Medical Systems).

Article Title: Progranulin as a Prognostic Biomarker for Breast Cancer Recurrence in Patients Who Had Hormone Receptor-Positive Tumors: A Cohort Study
Article Snippet: .. Staining was performed using the I-View DAB detection kit and a Ventana ES autostainer (Ventana Medical Systems, Tucson, AZ, USA) using primary antibodies against ER and PR (both from Ventana Medical Systems). ..

Article Title: Hemodynamic Response Imaging: A Potential Tool for the Assessment of Angiogenesis in Brain Tumors
Article Snippet: .. Immunohistochemical staining was performed on the Benchmark XT autostainer (Ventana Medical Systems Inc.) using the I-View DAB detection kit (Ventana). .. Statistical analysis Statistical analysis was performed on data obtained from the healthy subjects (n = 19) using SPSS 12.0 (SPSS Inc., Chicago, IL, USA).

Article Title: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections
Article Snippet: .. IHC determination of HER2 protein expression The FDA-approved HER2 IHC assay using PATHWAY HER-2/neu rabbit monoclonal antibody (clone 4B5; Ventana) was performed with i View DAB Detection Kit (Ventana) on a BenchMark XT automated staining system (Ventana). .. Briefly, the tissue sections were deparaffinized with EZ Prep (Ventana) at 75°C, heat pretreated in Cell Conditioning 1 (CC1; Ventana) using “standard cell conditioning” for antigen retrieval at 100°C, and then incubated with the anti-HER2 primary antibody for 32 min at 37°C after inactivation of the endogenous peroxidase with hydrogen peroxide for 4 min.

Article Title: Biological importance of podoplanin expression in chorionic villous stromal cells and its relationship to placental pathologies
Article Snippet: .. Immunohistochemical staining and evaluation Immunohistochemical staining was performed on a fully automatic immunostaining device (BenchMark XT; Ventana Medical Systems Inc., Tucson, AZ, USA) using podoplanin (Clone D2-40, diluted 1:100) and an I-View DAB detection kit (Ventana Medical Systems Inc.). .. Internal (endometrial blood and lymphatic vascular endothelium) and external (skin-dermal blood and lymphatic vascular endothelium) negative/positive control tissues were included in each staining.

Article Title: Desmoglein 1 deficiency results in severe dermatitis, multiple allergies and metabolic wasting
Article Snippet: For LEKTI and filaggrin staining, formaldehyde-fixed 5-μm paraffin-embedded sections were mounted on SuperFrost®Plus glass (Menzel-Glazer) and processed by using an automated immunostainer (Benchmark-XT, Ventana Medical System). .. Visualization of the bound primary antibodies was performed using the I-View DAB detection kit (Ventana Medical System).

Article Title: A Crucial Role for Kupffer Cell-Derived Galectin-9 in Regulation of T Cell Immunity in Hepatitis C Infection
Article Snippet: All of the staining steps were performed in the Ventana NexES autostainer (Ventana Medical Systems; Tucson, AZ) at 37°C. .. An I-VIEW DAB detection kit (Ventana) was used for antibody detection and included endogenous peroxidase block, secondary antibody, streptavidin horseradish peroxidase, DAB substrate with hydrogen peroxide activator and copper enhancer.

Article Title: Podocytes Contribute to the Formation of Glomerular Crescents
Article Snippet: Sections underwent heat-induced epitope retrieval and were blocked for endogenous peroxidase and biotin, as part of the automated staining protocol. .. The detection system used a rabbit biotinylated secondary antibody (Vector) with the Ventana i-VIEW DAB detection kit.

Article Title: Major Functional Transcriptome of an Inferred Center Regulator of an ER(-) Breast Cancer Model System
Article Snippet: Paragraph title: Immunohistochemical (IHC) stain of paraffin-embedded breast cancer slides ... For visualization of immunostain, we applied diaminobenzidine as a chromogen (i view™ DAB Detection Kit, Ventana Medical Systems, USA).

Immunostaining:

Article Title: Immunohistochemical Analysis of Oral Dysplasia: Diagnostic Assessment by Fascin and Podoplanin Expression
Article Snippet: .. The slides were incubated with specific primary antibodies raised against fascin (mouse monoclonal antibody, clone 55K-2; DAKO) at a 1:100 dilution and podoplanin (mouse monoclonal antibody, clone NZ-1; AngioBio, Del Mar, CA) at a 1:200 dilution at 37°C for 32 min. Immunostaining with an i VIEW DAB Detection Kit (Ventana Medical Systems Inc., AZ) and BenchMark XT (Ventana Medical Systems) was performed. ..

Article Title: Biological importance of podoplanin expression in chorionic villous stromal cells and its relationship to placental pathologies
Article Snippet: .. Immunohistochemical staining and evaluation Immunohistochemical staining was performed on a fully automatic immunostaining device (BenchMark XT; Ventana Medical Systems Inc., Tucson, AZ, USA) using podoplanin (Clone D2-40, diluted 1:100) and an I-View DAB detection kit (Ventana Medical Systems Inc.). .. Internal (endometrial blood and lymphatic vascular endothelium) and external (skin-dermal blood and lymphatic vascular endothelium) negative/positive control tissues were included in each staining.

Article Title: Podocytes Contribute to the Formation of Glomerular Crescents
Article Snippet: Mice were examined at 5.5 wk of age; 5-μm formalin-fixed, paraffin-embedded tissue sections were mounted on positively charged microscope slides and then baked overnight at 60°C before immunostaining. .. The detection system used a rabbit biotinylated secondary antibody (Vector) with the Ventana i-VIEW DAB detection kit.

Chromogenic In Situ Hybridization:

Article Title: Major Functional Transcriptome of an Inferred Center Regulator of an ER(-) Breast Cancer Model System
Article Snippet: For visualization of immunostain, we applied diaminobenzidine as a chromogen (i view™ DAB Detection Kit, Ventana Medical Systems, USA). .. For ERBB2 (IHC score: 2+), determination of Her-2/neu gene copy number by chromogenic in situ hybridization (CISH) was performed ( ).

Plasmid Preparation:

Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer
Article Snippet: Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana). .. Ki-67 and cleaved caspase-3 antibodies were incubated for 32 minutes and detected with a modified I-VIEW DAB detection kit (Ventana).

Article Title: Podocytes Contribute to the Formation of Glomerular Crescents
Article Snippet: .. The detection system used a rabbit biotinylated secondary antibody (Vector) with the Ventana i-VIEW DAB detection kit. .. Staining was performed on the Benchmark auto-immunostainer (Ventana).

Immunolabeling:

Article Title: Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation
Article Snippet: Immunolabeling of Ki-67 (1:500 rabbit mAb SP6, no. RM-9106-SO; Neomarkers/Pierce, Rockford, IL) was accomplished following a 32-min incubation with primary Ab at 37°C on a NexES stainer (Ventana Medical Systems, Tucson, AZ). .. Secondary detection employed a modification of the I-VIEW DAB detection kit (Ventana); specifically, the I-VIEW secondary antibody and enzyme were replaced with Rabbit ImmPress no. MP-7401 (Vector Laboratories, Burlingame, CA).

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    Ventana Medical avidin biotin complexed immunoperoxidase reaction
    Avidin Biotin Complexed Immunoperoxidase Reaction, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complexed immunoperoxidase reaction/product/Ventana Medical
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complexed immunoperoxidase reaction - by Bioz Stars, 2020-02
    78/100 stars
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