Structured Review

Kapa Biosystems hyper library preparation kit
Hyper Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hyper library preparation kit/product/Kapa Biosystems
Average 93 stars, based on 7 article reviews
Price from $9.99 to $1999.99
hyper library preparation kit - by Bioz Stars, 2020-05
93/100 stars

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Amplification:

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Construct:

Article Title: Brain Tumor Mutations Detected in Cerebral Spinal Fluid
Article Snippet: .. The Illumina libraries were constructed with Hyper Library Preparation Kit (Kapa Biosystems). .. The targeted DNA was enriched by the NimbleGene SeqCap EZ Comprehensive Cancer Design kit (Roche).

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

Polymerase Chain Reaction:

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Sequencing:

Article Title: Whole exome and whole genome sequencing with dried blood spot DNA without whole genome amplification
Article Snippet: .. The sequencing libraries were made using the KAPA HTP or Hyper Library Preparation Kit according to manufacturer’s instructions (Kapa Biosystems, Wilmington, MA, USA). .. For libraries optimized for larger inserts sizes, the ligation products were treated with the KAPA dual-SPRI size selection washing procedure.

Sonication:

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

Chromatin Immunoprecipitation:

Article Title: KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase
Article Snippet: .. In brief, indexed libraries were prepared from 20 ng Bioruptor-sheared (Diagenode) ChIP DNA using a Hyper Library Preparation Kit (Kapa Biosystems). .. The libraries were amplified using three cycles of PCR and then assessed for quality using a Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies) and quantified using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).

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    Kapa Biosystems hyper library preparation kit
    Hyper Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hyper library preparation kit/product/Kapa Biosystems
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    hyper library preparation kit - by Bioz Stars, 2020-05
    93/100 stars
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