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Kapa Biosystems hyper library preparation kit
Hyper Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hyper library preparation kit/product/Kapa Biosystems
Average 92 stars, based on 7 article reviews
Price from $9.99 to $1999.99
hyper library preparation kit - by Bioz Stars, 2019-10
92/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. Libraries were pooled in equimolar concentration and size selected on a 2% agarose gel for fragments 500 bp to 800 bp in length.

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The size-selected library was quantified with Qubit (ThermoFisher) and run on an Agilent bioanalyzer DNA high-sensitivity chip (Agilent, Santa Clara, CA, USA) to confirm the presence of DNA fragments of the expected size range.

Flow Cytometry:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The pool was further quantitated by quantitative PCR on a CFX Connect real-time system (Bio-Rad, Hercules, CA).

Amplification:

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Agarose Gel Electrophoresis:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

Microarray:

Article Title: KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase
Article Snippet: ChIP sequencing was performed at the Sequencing and Microarray Facility at The University of Texas MD Anderson Cancer Center. .. In brief, indexed libraries were prepared from 20 ng Bioruptor-sheared (Diagenode) ChIP DNA using a Hyper Library Preparation Kit (Kapa Biosystems).

Sequencing:

Article Title: KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase
Article Snippet: ChIP sequencing was performed at the Sequencing and Microarray Facility at The University of Texas MD Anderson Cancer Center. .. In brief, indexed libraries were prepared from 20 ng Bioruptor-sheared (Diagenode) ChIP DNA using a Hyper Library Preparation Kit (Kapa Biosystems).

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: Paragraph title: Genome sequencing and assembly. ... The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland).

Article Title: Brain Tumor Mutations Detected in Cerebral Spinal Fluid
Article Snippet: Paragraph title: CSF CANCER PANEL SEQUENCING ... The Illumina libraries were constructed with Hyper Library Preparation Kit (Kapa Biosystems).

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: Paragraph title: Sequence data generation ... A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification.

Construct:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

Article Title: Brain Tumor Mutations Detected in Cerebral Spinal Fluid
Article Snippet: Matched CSF total DNA and healthy tissue DNA were fragmented to approximately 200 bp by sonication before library construction. .. The Illumina libraries were constructed with Hyper Library Preparation Kit (Kapa Biosystems). .. The targeted DNA was enriched by the NimbleGene SeqCap EZ Comprehensive Cancer Design kit (Roche).

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Produced:

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: Sanger data : The sequence data set comprised of 30,111,484 Sanger reads for the Thoroughbred mare Twilight, produced for and used to build EquCab2 , were downloaded from the NCBI Trace Archive using the perl script query_tracedb downloaded from the NCBI website with the query string query page_size 30,000 page_number 223 binary SPECIES_CODE = EQUUS CABALLUS and CENTER_NAME = BI and CENTER_PROJECT = G836. .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification.

Concentration Assay:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

Incubation:

Article Title: KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase
Article Snippet: Chromatin prepared from cells in a 15-cm dish was used to determine total DNA input and was incubated overnight with specific antibodies or normal rabbit IgG. .. In brief, indexed libraries were prepared from 20 ng Bioruptor-sheared (Diagenode) ChIP DNA using a Hyper Library Preparation Kit (Kapa Biosystems).

Polyacrylamide Gel Electrophoresis:

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: Sanger data : The sequence data set comprised of 30,111,484 Sanger reads for the Thoroughbred mare Twilight, produced for and used to build EquCab2 , were downloaded from the NCBI Trace Archive using the perl script query_tracedb downloaded from the NCBI website with the query string query page_size 30,000 page_number 223 binary SPECIES_CODE = EQUUS CABALLUS and CENTER_NAME = BI and CENTER_PROJECT = G836. .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification.

Polymerase Chain Reaction:

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Sonication:

Article Title: Transcriptional and Functional Analysis of Bifidobacterium animalis subsp. lactis Exposure to Tetracycline
Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. The shotgun genomic DNA libraries were constructed from 500 ng of DNA after sonication with an ME220 ultrasonicator (Covaris, Woburn, MA) to an average fragment size of 500 base pairs (bp) with the Hyper library preparation kit from (Kapa Biosystems, Roche, Basel, Switzerland). .. The individually barcoded libraries were amplified with 3 cycles of PCR and run on a fragment analyzer (AATI, Ankeny, IA) to confirm the absence of free primers and primer dimers and to confirm the presence of DNA of the expected size range.

Article Title: Brain Tumor Mutations Detected in Cerebral Spinal Fluid
Article Snippet: Matched CSF total DNA and healthy tissue DNA were fragmented to approximately 200 bp by sonication before library construction. .. The Illumina libraries were constructed with Hyper Library Preparation Kit (Kapa Biosystems).

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: Carver Biotechnology Center, University of Illinois at Urbana-Champaign (UIUC). .. A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

Chromatin Immunoprecipitation:

Article Title: KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase
Article Snippet: ChIP sequencing was performed at the Sequencing and Microarray Facility at The University of Texas MD Anderson Cancer Center. .. In brief, indexed libraries were prepared from 20 ng Bioruptor-sheared (Diagenode) ChIP DNA using a Hyper Library Preparation Kit (Kapa Biosystems). .. The libraries were amplified using three cycles of PCR and then assessed for quality using a Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies) and quantified using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: A shotgun genomic DNA library with an insert size of 500 bp (range 300–650 bp) was constructed from 2 µg of Twilight’s genomic DNA after sonication with a Covaris M220 (Covaris, MA, USA) with the Hyper Library Preparation Kit from Kapa Biosystems (Roche) with no PCR amplification. .. The DNA library with adaptors was loaded onto a 2% agarose gel and fragments 450 to 550 bp in length were cut from the gel and recovered with the QIAquick Gel Extraction Kit (Qiagen, CA, USA).

ChIP-sequencing:

Article Title: KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase
Article Snippet: Paragraph title: ChIP and ChIP–seq assay ... In brief, indexed libraries were prepared from 20 ng Bioruptor-sheared (Diagenode) ChIP DNA using a Hyper Library Preparation Kit (Kapa Biosystems).

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    Kapa Biosystems kapa hyper prep library preparation kit
    Kapa Hyper Prep Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hyper prep library preparation kit/product/Kapa Biosystems
    Average 86 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    kapa hyper prep library preparation kit - by Bioz Stars, 2019-10
    86/100 stars
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