hydrophobic interaction liquid chromatography hilic column  (Agilent technologies)

 
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    Name:
    Zorbax
    Description:
    The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed phase HPLC ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution From ZORBAX Eclipse Plus C18 to sub 2 micron UHPLC columms a wide selection of stationary phases and particle sizes allows you to fine tune your selectivity to match your application
    Catalog Number:
    5188-5328
    Price:
    None
    Category:
    Products Small Molecule Columns Reversed Phase Hplc Columns Zorbax
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    Structured Review

    Agilent technologies hydrophobic interaction liquid chromatography hilic column
    The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed phase HPLC ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution From ZORBAX Eclipse Plus C18 to sub 2 micron UHPLC columms a wide selection of stationary phases and particle sizes allows you to fine tune your selectivity to match your application
    https://www.bioz.com/result/hydrophobic interaction liquid chromatography hilic column/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydrophobic interaction liquid chromatography hilic column - by Bioz Stars, 2021-07
    97/100 stars

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    Related Articles

    Purification:

    Article Title: Development of a selective agonist for relaxin family peptide receptor 3
    Article Snippet: Refolded precursors were purified by HPLC using a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm, Agilent Technology, Santa Clara, CA, USA), and then sequentially treated with endoproteinase Lys-C, papaya glutaminyl cyclase, and carboxypeptidase B, as described in our previous studies , . .. Finally, mature R3/I5 mutants were purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm, Agilent Technology) and their identity confirmed by electrospray mass spectrometry on a QTRAP mass spectrometer (Applied Biosystems, Foster City, CA, USA). .. Circular dichroism spectroscopy Mature R3/I5 mutants were dissolved in 1.0 mM aqueous hydrochloride solution (pH 3.0) and their concentrations were determined by absorbance at 280 nm using an extinction coefficient (ε280nm ) of 7365 M−1 ·cm−1 .

    Article Title: Antioxidant Peptides from Collagen Hydrolysate of Redlip Croaker (Pseudosciaena polyactis) Scales: Preparation, Characterization, and Cytoprotective Effects on H2O2-Damaged HepG2 Cells
    Article Snippet: .. Each eluate (3 mL) was collected and monitored at 214 nm, and the fraction of GFC-2 solution (25 μL, 10.0 mg/mL) was further purified on an Agilent 1260 HPLC system (Agilent Ltd., Santa Rosa, CA, USA) with an Agilent Zorbax, SB C-18 column (4.6 mm × 250 mm). .. The sample was eluated with a linear gradient of ACN (0%–50% in 0–40 min) in 0.1% TFA at a flow rate of 0.8 mL/min, and six CPs (RCP1 to RCP6) were isolated on the absorbance at 214 nm.

    High Performance Liquid Chromatography:

    Article Title: Development of a selective agonist for relaxin family peptide receptor 3
    Article Snippet: Refolded precursors were purified by HPLC using a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm, Agilent Technology, Santa Clara, CA, USA), and then sequentially treated with endoproteinase Lys-C, papaya glutaminyl cyclase, and carboxypeptidase B, as described in our previous studies , . .. Finally, mature R3/I5 mutants were purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm, Agilent Technology) and their identity confirmed by electrospray mass spectrometry on a QTRAP mass spectrometer (Applied Biosystems, Foster City, CA, USA). .. Circular dichroism spectroscopy Mature R3/I5 mutants were dissolved in 1.0 mM aqueous hydrochloride solution (pH 3.0) and their concentrations were determined by absorbance at 280 nm using an extinction coefficient (ε280nm ) of 7365 M−1 ·cm−1 .

    Article Title: Antioxidant Peptides from Collagen Hydrolysate of Redlip Croaker (Pseudosciaena polyactis) Scales: Preparation, Characterization, and Cytoprotective Effects on H2O2-Damaged HepG2 Cells
    Article Snippet: .. Each eluate (3 mL) was collected and monitored at 214 nm, and the fraction of GFC-2 solution (25 μL, 10.0 mg/mL) was further purified on an Agilent 1260 HPLC system (Agilent Ltd., Santa Rosa, CA, USA) with an Agilent Zorbax, SB C-18 column (4.6 mm × 250 mm). .. The sample was eluated with a linear gradient of ACN (0%–50% in 0–40 min) in 0.1% TFA at a flow rate of 0.8 mL/min, and six CPs (RCP1 to RCP6) were isolated on the absorbance at 214 nm.

    Article Title: Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2
    Article Snippet: Each eluate (3 mL) was collected, monitored at 214 nm, and measured the DPPH· and hydroxyl radical (HO·) scavenging activity. .. Two subfractions (MUA-4-A and MUA-4-B) were collected and MUA-4-B with higher DPPH· and HO· scavenging activity was isolated using a Zorbax, SB C-18 column (4.6 × 250 mm, 5 µm) in an Agilent 1260 HPLC system. ..

    Mass Spectrometry:

    Article Title: Development of a selective agonist for relaxin family peptide receptor 3
    Article Snippet: Refolded precursors were purified by HPLC using a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm, Agilent Technology, Santa Clara, CA, USA), and then sequentially treated with endoproteinase Lys-C, papaya glutaminyl cyclase, and carboxypeptidase B, as described in our previous studies , . .. Finally, mature R3/I5 mutants were purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm, Agilent Technology) and their identity confirmed by electrospray mass spectrometry on a QTRAP mass spectrometer (Applied Biosystems, Foster City, CA, USA). .. Circular dichroism spectroscopy Mature R3/I5 mutants were dissolved in 1.0 mM aqueous hydrochloride solution (pH 3.0) and their concentrations were determined by absorbance at 280 nm using an extinction coefficient (ε280nm ) of 7365 M−1 ·cm−1 .

    Injection:

    Article Title: Clove Extract Inhibits Tumor Growth and Promotes Cell Cycle Arrest and Apoptosis
    Article Snippet: Analytical standards of oleanolic acid (OA), ursolic acid (UA), eugenol (Sigma, St. Louis, MO, USA), and EAEC were analyzed by HPLC based on a previously described assay ( ). .. Briefly, extracts were resuspended in 60% methanol and injected onto a C18 column (RP-18, Agilent Zorbax Eclipse XDB-C18 3.0×150 mm, 3.5 μm) with a C18 guard column (RP18, Applied Biosciences). .. The mobile phase was composed of methanol with 0.2% ammonium acetate (solvent A) and water with 0.2% ammonium acetate, pH 6.6 (solvent B), with the following gradient: 0–5 min, 60% solvent A; 5–35 min, 60–90% solvent A linearly; 35–45 min, 90% solvent A, at a flow rate of 0.5 ml/min.

    Article Title: Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandem-mass Spectrometry
    Article Snippet: MassHunter Workstation software was used for data analysis. .. The 5-AIQC-tagged samples (1 μL) were individually injected on an UHPLC column (Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD, 2.1 × 100 mm, 1.8 μm) with its temperature set to 50 °C. .. Ultrapure water (MilliQ) and methanol containing 0.1% (v/v) formic acid were used as two mobile phases A and B, respectively, with flow rate of 0.6 mL/min.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Melatonin exerts oncostatic capacity and decreases melanogenesis in human MNT-1 melanoma cells
    Article Snippet: Resulting samples were dried with liquid nitrogen, redissoved in methanol followed by LC-MS analysis using Xevo G2-XS QTof LC-MS system (Waters). .. Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology) and Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters) were used for LC-MS analysis. ..

    Activity Assay:

    Article Title: Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2
    Article Snippet: Each eluate (3 mL) was collected, monitored at 214 nm, and measured the DPPH· and hydroxyl radical (HO·) scavenging activity. .. Two subfractions (MUA-4-A and MUA-4-B) were collected and MUA-4-B with higher DPPH· and HO· scavenging activity was isolated using a Zorbax, SB C-18 column (4.6 × 250 mm, 5 µm) in an Agilent 1260 HPLC system. ..

    Isolation:

    Article Title: Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2
    Article Snippet: Each eluate (3 mL) was collected, monitored at 214 nm, and measured the DPPH· and hydroxyl radical (HO·) scavenging activity. .. Two subfractions (MUA-4-A and MUA-4-B) were collected and MUA-4-B with higher DPPH· and HO· scavenging activity was isolated using a Zorbax, SB C-18 column (4.6 × 250 mm, 5 µm) in an Agilent 1260 HPLC system. ..

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