hydrophilic interaction liquid chromatography hilic fractionation  (Tosoh Corporation)

 
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    Structured Review

    Tosoh Corporation hydrophilic interaction liquid chromatography hilic fractionation
    Hydrophilic Interaction Liquid Chromatography Hilic Fractionation, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydrophilic interaction liquid chromatography hilic fractionation/product/Tosoh Corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydrophilic interaction liquid chromatography hilic fractionation - by Bioz Stars, 2020-07
    93/100 stars

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    Hydrophilic Interaction Liquid Chromatography:

    Article Title: First-trimester proteomic profiling identifies novel predictors of gestational diabetes mellitus
    Article Snippet: .. For each TMT 10-plex experiment all samples were pooled in 1:1 ratios and 50 μg was purified on custom made Poros R2/R3 (Thermo Scientific) micro columns, dried, reconstituted and subjected to hydrophilic interaction liquid chromatography (HILIC) fractionation (13–17 fractions) on a TSK Amide-80 3 μm column (Tosoh Bioscience, Stuttgart, Germany) using a Agilent 1200 series high performance liquid chromatography (HPLC) system (Agilent, Santa Clara, CA, US) [ , ]. .. The lyophilized fractions were reconstituted in 0.1% Formic acid (FA) and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) on an Easy 1000 nano-flow LC/orbitrap Q Exactive HF system (Thermo Scientific) using a custom made 2 column setup (Reprosil-Pur 120 C18-AQ, Dr. Maisch, Ammerbuch-Entringen, Germany), a two hour gradient and a top 20 shot gun proteomics setup.

    High Performance Liquid Chromatography:

    Article Title: First-trimester proteomic profiling identifies novel predictors of gestational diabetes mellitus
    Article Snippet: .. For each TMT 10-plex experiment all samples were pooled in 1:1 ratios and 50 μg was purified on custom made Poros R2/R3 (Thermo Scientific) micro columns, dried, reconstituted and subjected to hydrophilic interaction liquid chromatography (HILIC) fractionation (13–17 fractions) on a TSK Amide-80 3 μm column (Tosoh Bioscience, Stuttgart, Germany) using a Agilent 1200 series high performance liquid chromatography (HPLC) system (Agilent, Santa Clara, CA, US) [ , ]. .. The lyophilized fractions were reconstituted in 0.1% Formic acid (FA) and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) on an Easy 1000 nano-flow LC/orbitrap Q Exactive HF system (Thermo Scientific) using a custom made 2 column setup (Reprosil-Pur 120 C18-AQ, Dr. Maisch, Ammerbuch-Entringen, Germany), a two hour gradient and a top 20 shot gun proteomics setup.

    Fractionation:

    Article Title: First-trimester proteomic profiling identifies novel predictors of gestational diabetes mellitus
    Article Snippet: .. For each TMT 10-plex experiment all samples were pooled in 1:1 ratios and 50 μg was purified on custom made Poros R2/R3 (Thermo Scientific) micro columns, dried, reconstituted and subjected to hydrophilic interaction liquid chromatography (HILIC) fractionation (13–17 fractions) on a TSK Amide-80 3 μm column (Tosoh Bioscience, Stuttgart, Germany) using a Agilent 1200 series high performance liquid chromatography (HPLC) system (Agilent, Santa Clara, CA, US) [ , ]. .. The lyophilized fractions were reconstituted in 0.1% Formic acid (FA) and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) on an Easy 1000 nano-flow LC/orbitrap Q Exactive HF system (Thermo Scientific) using a custom made 2 column setup (Reprosil-Pur 120 C18-AQ, Dr. Maisch, Ammerbuch-Entringen, Germany), a two hour gradient and a top 20 shot gun proteomics setup.

    Purification:

    Article Title: First-trimester proteomic profiling identifies novel predictors of gestational diabetes mellitus
    Article Snippet: .. For each TMT 10-plex experiment all samples were pooled in 1:1 ratios and 50 μg was purified on custom made Poros R2/R3 (Thermo Scientific) micro columns, dried, reconstituted and subjected to hydrophilic interaction liquid chromatography (HILIC) fractionation (13–17 fractions) on a TSK Amide-80 3 μm column (Tosoh Bioscience, Stuttgart, Germany) using a Agilent 1200 series high performance liquid chromatography (HPLC) system (Agilent, Santa Clara, CA, US) [ , ]. .. The lyophilized fractions were reconstituted in 0.1% Formic acid (FA) and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) on an Easy 1000 nano-flow LC/orbitrap Q Exactive HF system (Thermo Scientific) using a custom made 2 column setup (Reprosil-Pur 120 C18-AQ, Dr. Maisch, Ammerbuch-Entringen, Germany), a two hour gradient and a top 20 shot gun proteomics setup.

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  • 93
    Tosoh Corporation hydrophilic interaction liquid chromatography hilic fractionation
    Hydrophilic Interaction Liquid Chromatography Hilic Fractionation, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydrophilic interaction liquid chromatography hilic fractionation/product/Tosoh Corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydrophilic interaction liquid chromatography hilic fractionation - by Bioz Stars, 2020-07
    93/100 stars
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