hybridization buffer  (Millipore)


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    Name:
    Hybridization Buffer
    Description:
    Hybridization buffer is used in combination with the PCR ELISA enzyme linked immunosorbent assay and the series of DIG Detection ELISAs for nucleic acid hybridization reactions
    Catalog Number:
    11717472001
    Price:
    None
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    Structured Review

    Millipore hybridization buffer
    Hybridization buffer is used in combination with the PCR ELISA enzyme linked immunosorbent assay and the series of DIG Detection ELISAs for nucleic acid hybridization reactions
    https://www.bioz.com/result/hybridization buffer/product/Millipore
    Average 99 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    hybridization buffer - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Microarray:

    Article Title: Genome-wide Identification and Quantitative Analysis of Cleaved tRNA Fragments Induced by Cellular Stress *
    Article Snippet: .. The labeled tiRNA sample was then dissolved in microarray hybridization buffer (Sigma-Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. This was then applied to the hybridization chamber of the GeneTAC Hyb4 station (Genomic Solutions, Ann Arbor, MI).

    Article Title: Genome-wide analysis of N1-methyl-adenosine modification in human tRNAs
    Article Snippet: .. The reverse-transcribed RNA samples (corresponding to ∼2 μg of total RNA) were dissolved in microarray hybridization buffer (Sigma Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. The sample was applied to the Hybridization chamber of the GeneTAC Hyb4 station (Genomic solutions).

    Article Title: Microarray Analysis Confirms the Specificity of a Chlamydomonas reinhardtii Chloroplast RNA Stability Mutant 1
    Article Snippet: .. Experimental and reference fluorescent-labeled cDNAs were combined in 22 μ L hybridization buffer (see above), denatured at 95°C for 5 min, cooled to room temperature for 2 min, and then placed over the microarray, covered with a 22 × 22 mm coverslip (Z36, 590-4 Hybri-Slips, Sigma, St. Louis), and incubated in a sealed chamber (Corning, CMT Hybridization chambers 2551) at 42°C for 20 h. Slides were washed at room temperature in 2× SSC, 0.25% SDS to remove coverslips, followed by a 10-min wash in the same buffer at 42°C with vigorous agitation. ..

    Article Title: Codon optimality controls differential mRNA translation during amino acid starvation
    Article Snippet: .. Briefly, the labeled tRNA sample was first dissolved in microarray hybridization buffer (Sigma-Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. This mixture was then applied to the hybridization chamber of the GeneTAC Hyb4 station (Genomic Solutions).

    Incubation:

    Article Title: Microarray Analysis Confirms the Specificity of a Chlamydomonas reinhardtii Chloroplast RNA Stability Mutant 1
    Article Snippet: .. Experimental and reference fluorescent-labeled cDNAs were combined in 22 μ L hybridization buffer (see above), denatured at 95°C for 5 min, cooled to room temperature for 2 min, and then placed over the microarray, covered with a 22 × 22 mm coverslip (Z36, 590-4 Hybri-Slips, Sigma, St. Louis), and incubated in a sealed chamber (Corning, CMT Hybridization chambers 2551) at 42°C for 20 h. Slides were washed at room temperature in 2× SSC, 0.25% SDS to remove coverslips, followed by a 10-min wash in the same buffer at 42°C with vigorous agitation. ..

    Labeling:

    Article Title: Genome-wide Identification and Quantitative Analysis of Cleaved tRNA Fragments Induced by Cellular Stress *
    Article Snippet: .. The labeled tiRNA sample was then dissolved in microarray hybridization buffer (Sigma-Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. This was then applied to the hybridization chamber of the GeneTAC Hyb4 station (Genomic Solutions, Ann Arbor, MI).

    Article Title: Codon optimality controls differential mRNA translation during amino acid starvation
    Article Snippet: .. Briefly, the labeled tRNA sample was first dissolved in microarray hybridization buffer (Sigma-Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. This mixture was then applied to the hybridization chamber of the GeneTAC Hyb4 station (Genomic Solutions).

    Blocking Assay:

    Article Title: Differential expression of miR-184 in temporal lobe epilepsy patients with and without hippocampal sclerosis – Influence on microglial function
    Article Snippet: .. Slides were then rinsed in hybridization buffer (5× SSC, 50% formamide and 5× Denhardt’s, 250 μg/ml yeast t-RNA (Sigma) 2% blocking reagent (Roche), 0.1% Tween 20) for 2 h at RT. .. Probes (4 pmol) were incubated in hybridization buffer overnight at Tm −20 °C (Tm provided by Exiqon) in a humidified chamber. miR-124 served as positive control for hybridization and miR-scr served as negative control.

    Hybridization:

    Article Title: Microsporidia Interact with Host Cell Mitochondria via Voltage-Dependent Anion Channels Using Sporoplasm Surface Protein 1
    Article Snippet: .. Briefly, coverslips in the 24-well plates were washed gently with PBS to remove free spores and fixed with 1:1 methanol/acetone for 10 min at room temperature, after which the fixative was removed and the coverslips were allowed to air-dry at room temperature for 30 min. Prehybridization of the coverslips was performed with 100 μl hybridization buffer (Sigma-Aldrich catalog no. 11717472001) for 1 h at 57°C in a humid chamber. .. Hybridization was then carried out by removing the prehybridization solution and then adding a 20 μM concentration of HEL878F probe (Alexa Fluor 594 ACTCTCACACTCACTTCAG [Thermo Fisher Scientific HPLC-purified custom oligonucleotide]) to hybridization buffer at 57°C overnight on a circular rocking platform in a humid chamber.

    Article Title: Efficient CRISPR/Cas9-mediated editing of trinucleotide repeat expansion in myotonic dystrophy patient-derived iPS and myogenic cells
    Article Snippet: .. After the rehydration step, the cells were hybridized in a hybridization chamber with custom Peptide Nucleic Acid (PNA)-probe (CY3–5′-CAGCAGCAGCAGCAG-3′ ) (Eurogentec) at a dilution of 1:500 in hybridization buffer [0.1% BSA (Sigma Aldrich) in water, 50% volume of formamide (Thermo Scientific) and 25% volume of SSC Buffer (Life Technologies)] for 90 min at 37°C. .. In the next step, the cells were washed with 0.1% tween 20 (Sigma Aldrich) in PBS for 5 min at RT.

    Article Title: Genome-wide Identification and Quantitative Analysis of Cleaved tRNA Fragments Induced by Cellular Stress *
    Article Snippet: .. The labeled tiRNA sample was then dissolved in microarray hybridization buffer (Sigma-Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. This was then applied to the hybridization chamber of the GeneTAC Hyb4 station (Genomic Solutions, Ann Arbor, MI).

    Article Title: Genome-wide analysis of N1-methyl-adenosine modification in human tRNAs
    Article Snippet: .. The reverse-transcribed RNA samples (corresponding to ∼2 μg of total RNA) were dissolved in microarray hybridization buffer (Sigma Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. The sample was applied to the Hybridization chamber of the GeneTAC Hyb4 station (Genomic solutions).

    Article Title: Microarray Analysis Confirms the Specificity of a Chlamydomonas reinhardtii Chloroplast RNA Stability Mutant 1
    Article Snippet: .. Experimental and reference fluorescent-labeled cDNAs were combined in 22 μ L hybridization buffer (see above), denatured at 95°C for 5 min, cooled to room temperature for 2 min, and then placed over the microarray, covered with a 22 × 22 mm coverslip (Z36, 590-4 Hybri-Slips, Sigma, St. Louis), and incubated in a sealed chamber (Corning, CMT Hybridization chambers 2551) at 42°C for 20 h. Slides were washed at room temperature in 2× SSC, 0.25% SDS to remove coverslips, followed by a 10-min wash in the same buffer at 42°C with vigorous agitation. ..

    Article Title: Nitric Oxide and the Oxytocin System in Pregnancy
    Article Snippet: .. The sections were hybridized for 17 hr at 37°C in 40 μl of hybridization buffer [4× SSC, 50% formamide, 1× Denhardt's solution, 500 μg/ml sheared salmon sperm DNA (Sigma), 10% dextran sulfate, and 0.3% mercaptoethanol] containing35 S-labeled NOS oligonucleotide probes at 2500 dpm/μl. .. The slides were stringently washed in SSC at 55°C, dipped in 300 m m ammonium acetate, 70% ethanol, air-dried, placed in autoradiographic cassettes, and exposed to Hyperfilm-βmax autoradiography film (Amersham Pharmacia Biotech) with35 S brain paste standards.

    Article Title: Codon optimality controls differential mRNA translation during amino acid starvation
    Article Snippet: .. Briefly, the labeled tRNA sample was first dissolved in microarray hybridization buffer (Sigma-Aldrich) containing 20 μg of salmon sperm DNA and 10 μg of poly(A). .. This mixture was then applied to the hybridization chamber of the GeneTAC Hyb4 station (Genomic Solutions).

    Article Title: Differential expression of miR-184 in temporal lobe epilepsy patients with and without hippocampal sclerosis – Influence on microglial function
    Article Snippet: .. Slides were then rinsed in hybridization buffer (5× SSC, 50% formamide and 5× Denhardt’s, 250 μg/ml yeast t-RNA (Sigma) 2% blocking reagent (Roche), 0.1% Tween 20) for 2 h at RT. .. Probes (4 pmol) were incubated in hybridization buffer overnight at Tm −20 °C (Tm provided by Exiqon) in a humidified chamber. miR-124 served as positive control for hybridization and miR-scr served as negative control.

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  • 99
    Millipore hybridization buffer
    Hybridization Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybridization buffer/product/Millipore
    Average 99 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    hybridization buffer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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