hybridisation buffer  (Millipore)


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    Name:
    Microarray Hybridisation Buffer Version 2
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    Catalog Number:
    gerpk0325
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    Structured Review

    Millipore hybridisation buffer
    Double labelling of a mixture of four mRNAs (A, C) and distinct genes: cyclin B1 mRNA (D, F), peroxidase mRNA (G, I), cytokinin-specific binding protein mRNA (J, L) and pectin methylesterase mRNA (M, O), with Sm proteins (B, E, H, K, N) in Lupinus cells. The arrows indicate colocalisation of mRNA transcripts with CBs. A stronger signal was observed in the cytoplasm than in the nuclei after in situ <t>hybridisation</t> with the mixture of probes (A, C) and with a probe against cytokinin-specific binding protein mRNA (J, L). % indicates the percentage of nuclei showing the representative immunolocalisation pattern. Bar, 5 µm. N-nucleus, Nu- nucleolus, C- cytoplasm.

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    Images

    1) Product Images from "Poly(A) RNAs Including Coding Proteins RNAs Occur in Plant Cajal Bodies"

    Article Title: Poly(A) RNAs Including Coding Proteins RNAs Occur in Plant Cajal Bodies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111780

    Double labelling of a mixture of four mRNAs (A, C) and distinct genes: cyclin B1 mRNA (D, F), peroxidase mRNA (G, I), cytokinin-specific binding protein mRNA (J, L) and pectin methylesterase mRNA (M, O), with Sm proteins (B, E, H, K, N) in Lupinus cells. The arrows indicate colocalisation of mRNA transcripts with CBs. A stronger signal was observed in the cytoplasm than in the nuclei after in situ hybridisation with the mixture of probes (A, C) and with a probe against cytokinin-specific binding protein mRNA (J, L). % indicates the percentage of nuclei showing the representative immunolocalisation pattern. Bar, 5 µm. N-nucleus, Nu- nucleolus, C- cytoplasm.
    Figure Legend Snippet: Double labelling of a mixture of four mRNAs (A, C) and distinct genes: cyclin B1 mRNA (D, F), peroxidase mRNA (G, I), cytokinin-specific binding protein mRNA (J, L) and pectin methylesterase mRNA (M, O), with Sm proteins (B, E, H, K, N) in Lupinus cells. The arrows indicate colocalisation of mRNA transcripts with CBs. A stronger signal was observed in the cytoplasm than in the nuclei after in situ hybridisation with the mixture of probes (A, C) and with a probe against cytokinin-specific binding protein mRNA (J, L). % indicates the percentage of nuclei showing the representative immunolocalisation pattern. Bar, 5 µm. N-nucleus, Nu- nucleolus, C- cytoplasm.

    Techniques Used: Binding Assay, In Situ, Hybridization

    Analysis of the fluorescence intensity resulting from in situ hybridisation of poly(A) RNA in the meristematic cells of lupines roots. There were significant differences in intensity (p
    Figure Legend Snippet: Analysis of the fluorescence intensity resulting from in situ hybridisation of poly(A) RNA in the meristematic cells of lupines roots. There were significant differences in intensity (p

    Techniques Used: Fluorescence, In Situ, Hybridization

    2) Product Images from "Cellular, Molecular and Functional Characterisation of YAC Transgenic Mouse Models of Friedreich Ataxia"

    Article Title: Cellular, Molecular and Functional Characterisation of YAC Transgenic Mouse Models of Friedreich Ataxia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0107416

    Transgene copy number. ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.
    Figure Legend Snippet: Transgene copy number. ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.

    Techniques Used: Negative Control, Transgenic Assay, Fluorescence In Situ Hybridization, Staining, Hybridization

    3) Product Images from "Effects of lipopolysaccharide-induced inflammation on expression of growth-associated genes by corticospinal neurons"

    Article Title: Effects of lipopolysaccharide-induced inflammation on expression of growth-associated genes by corticospinal neurons

    Journal: BMC Neuroscience

    doi: 10.1186/1471-2202-7-8

    GAP-43 mRNA expression after LPS application . Coronal sections of motor cortex hybridised with GAP-43 mRNA probe 3 days (Figs 9a – d), 7 days (Figs 9e, f) and 1 month (Figs 9g, h) after unilateral application of LPS (Figs 9b, d, f, h) or sham operations to the contralateral (control) side (Figs 9a, c, e, g). Background levels of GAP-43 mRNA are seen in contralateral (control) cortex at 3 days but stronger expression is apparent in layers II–V of LPS-treated cortex. Areas of layer V in Fig. 9a and b are enlarged in Fig. 9c and d to better show differences in hybridisation signals. By 7 days (Figs 9e and 9f), GAP-43 mRNA expression appears to be identical on both sides, and remains so one month after LPS application (Figs 9g and h). Scale bar in Fig. 9a = 500 μm and also applies to Fig. 9b; scale bar in Fig. 9c = 50 μm and also applies to Fig. 9d; scale bar in Fig. 9e = 200 μm and also applies to Figs 9f – h.
    Figure Legend Snippet: GAP-43 mRNA expression after LPS application . Coronal sections of motor cortex hybridised with GAP-43 mRNA probe 3 days (Figs 9a – d), 7 days (Figs 9e, f) and 1 month (Figs 9g, h) after unilateral application of LPS (Figs 9b, d, f, h) or sham operations to the contralateral (control) side (Figs 9a, c, e, g). Background levels of GAP-43 mRNA are seen in contralateral (control) cortex at 3 days but stronger expression is apparent in layers II–V of LPS-treated cortex. Areas of layer V in Fig. 9a and b are enlarged in Fig. 9c and d to better show differences in hybridisation signals. By 7 days (Figs 9e and 9f), GAP-43 mRNA expression appears to be identical on both sides, and remains so one month after LPS application (Figs 9g and h). Scale bar in Fig. 9a = 500 μm and also applies to Fig. 9b; scale bar in Fig. 9c = 50 μm and also applies to Fig. 9d; scale bar in Fig. 9e = 200 μm and also applies to Figs 9f – h.

    Techniques Used: Expressing, Hybridization

    4) Product Images from "Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones"

    Article Title: Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones

    Journal: Molecular Pain

    doi: 10.1186/1744-8069-6-61

    Expression of PAR1-4 in sections of adult mouse DRG . A. In situ hybridisation (ISH) for PAR1-4 carried out as described in Methods. Positive cells shown by arrowheads. Sections counterstained using hematoxylin-eosin. Scale bars 40 μm. B. Similar sections in which PAR1, 3 and 4 expression was determined using immunohistochemistry. Positive cells shown with arrows. The PAR2 antibodies available to us were found to be non-specific on Western blots and results for PAR2 are therefore not shown. Sections counterstained using hematoxylin-eosin. Scale bars 40 μm. C. Expression of PAR1 - 4 as a function of neuronal size in adult mouse DRG using ISH. Overall neuronal population (grey) is compared with those positive for each PAR isoform (black). Overall, PAR1 was found to be expressed in 15.0% of neurones, PAR2 in 21.5%, PAR3 in 49.5% and PAR4 in 14.5%. D. Similar results obtained using immunohistochemistry. PAR2 is not shown because the antibody was found to exhibit non-specific binding, and PAR4 is not shown because it proved impossible to distinguish neuronal from glial cell staining (see B). Overall PAR1 was expressed in 10.3% of neurones, and PAR3 in 42.0%.
    Figure Legend Snippet: Expression of PAR1-4 in sections of adult mouse DRG . A. In situ hybridisation (ISH) for PAR1-4 carried out as described in Methods. Positive cells shown by arrowheads. Sections counterstained using hematoxylin-eosin. Scale bars 40 μm. B. Similar sections in which PAR1, 3 and 4 expression was determined using immunohistochemistry. Positive cells shown with arrows. The PAR2 antibodies available to us were found to be non-specific on Western blots and results for PAR2 are therefore not shown. Sections counterstained using hematoxylin-eosin. Scale bars 40 μm. C. Expression of PAR1 - 4 as a function of neuronal size in adult mouse DRG using ISH. Overall neuronal population (grey) is compared with those positive for each PAR isoform (black). Overall, PAR1 was found to be expressed in 15.0% of neurones, PAR2 in 21.5%, PAR3 in 49.5% and PAR4 in 14.5%. D. Similar results obtained using immunohistochemistry. PAR2 is not shown because the antibody was found to exhibit non-specific binding, and PAR4 is not shown because it proved impossible to distinguish neuronal from glial cell staining (see B). Overall PAR1 was expressed in 10.3% of neurones, and PAR3 in 42.0%.

    Techniques Used: Expressing, In Situ, Hybridization, In Situ Hybridization, Immunohistochemistry, Western Blot, Binding Assay, Staining

    5) Product Images from "Impact of humanised isolation and culture conditions on stemness and osteogenic potential of bone marrow derived mesenchymal stromal cells"

    Article Title: Impact of humanised isolation and culture conditions on stemness and osteogenic potential of bone marrow derived mesenchymal stromal cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52442-9

    Human cell identification by in situ hybridisation of the human-specific repetitive Alu sequence. ( a) Micrographs showing human cells 1 week post-implantation populated with host cells (purple nuclei) and human cells (brown nuclei) surrounding BCP granules in both Direct(AB + FGF high ) and Direct(PL) (black arrows). Human cells populating dense connective tissue in Direct(PL) compared to a loose connective tissue in Direct(AB + FGF high ). (b) Human cells identified by the brown nuclei (black arrows) in osteocyte lacunae (black arrows) within the mineralised bone formed in Direct(AB + FGF high ) after 11 weeks post implantation. Scale 100 µm.
    Figure Legend Snippet: Human cell identification by in situ hybridisation of the human-specific repetitive Alu sequence. ( a) Micrographs showing human cells 1 week post-implantation populated with host cells (purple nuclei) and human cells (brown nuclei) surrounding BCP granules in both Direct(AB + FGF high ) and Direct(PL) (black arrows). Human cells populating dense connective tissue in Direct(PL) compared to a loose connective tissue in Direct(AB + FGF high ). (b) Human cells identified by the brown nuclei (black arrows) in osteocyte lacunae (black arrows) within the mineralised bone formed in Direct(AB + FGF high ) after 11 weeks post implantation. Scale 100 µm.

    Techniques Used: In Situ, Hybridization, Sequencing

    6) Product Images from "Expression profiling in vivo demonstrates rapid changes in lung microRNA levels following lipopolysaccharide-induced inflammation but not in the anti-inflammatory action of glucocorticoids"

    Article Title: Expression profiling in vivo demonstrates rapid changes in lung microRNA levels following lipopolysaccharide-induced inflammation but not in the anti-inflammatory action of glucocorticoids

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-8-240

    LPS-induced changes in miRNA-223 expression in the mouse lung. Lungs were removed from saline-challenged (A-D) and LPS-challenged (E-H) mice at 3 hrs post-challenge and tissue slices were examined by in situ hybridisation using either a miRNA-223-specific (A, B, E, F) or scrambled (C, G) LNA probe, or histologically following cresyl violet staining (D, H). Bronchial (BE) and alveolar (AE) epithelia, as well as the vascular endothelium (VE) and leukocytes (L) are indicated.
    Figure Legend Snippet: LPS-induced changes in miRNA-223 expression in the mouse lung. Lungs were removed from saline-challenged (A-D) and LPS-challenged (E-H) mice at 3 hrs post-challenge and tissue slices were examined by in situ hybridisation using either a miRNA-223-specific (A, B, E, F) or scrambled (C, G) LNA probe, or histologically following cresyl violet staining (D, H). Bronchial (BE) and alveolar (AE) epithelia, as well as the vascular endothelium (VE) and leukocytes (L) are indicated.

    Techniques Used: Expressing, Mouse Assay, In Situ, Hybridization, Staining

    7) Product Images from "Guidelines for Optimisation of a Multiplex Oligonucleotide Ligation-PCR for Characterisation of Microbial Pathogens in a Microsphere Suspension Array"

    Article Title: Guidelines for Optimisation of a Multiplex Oligonucleotide Ligation-PCR for Characterisation of Microbial Pathogens in a Microsphere Suspension Array

    Journal: BioMed Research International

    doi: 10.1155/2015/790170

    Boxplot of signal-to-noise ratios for different combinations of amount of microspheres in the hybridisation reaction and streptavidin-R-phycoerythrin (SAPE) concentration in the reporter mix. Results of one of 2 independent tests are presented, that is, results of 6 isolates × 6 conditions = 36 MOL-PCR assays (for each of the 6 conditions: n total = 120 with n neg = 64 and n pos = 56). 2500: 2500 microspheres of each region per reaction were combined in the microsphere mix; 375: 375 microspheres of each region per reaction were combined in the microsphere mix; 750: 750 microspheres of each region per reaction were combined in the microsphere mix; 4 µ g: the reporter mix contained 4 μ g/mL of SAPE; 10 µ g: the reporter mix contained 10 μ g/mL of SAPE; neg: expected negative results; pos: expected positive results; R: reference condition; * : statistically significant difference with the reference condition.
    Figure Legend Snippet: Boxplot of signal-to-noise ratios for different combinations of amount of microspheres in the hybridisation reaction and streptavidin-R-phycoerythrin (SAPE) concentration in the reporter mix. Results of one of 2 independent tests are presented, that is, results of 6 isolates × 6 conditions = 36 MOL-PCR assays (for each of the 6 conditions: n total = 120 with n neg = 64 and n pos = 56). 2500: 2500 microspheres of each region per reaction were combined in the microsphere mix; 375: 375 microspheres of each region per reaction were combined in the microsphere mix; 750: 750 microspheres of each region per reaction were combined in the microsphere mix; 4 µ g: the reporter mix contained 4 μ g/mL of SAPE; 10 µ g: the reporter mix contained 10 μ g/mL of SAPE; neg: expected negative results; pos: expected positive results; R: reference condition; * : statistically significant difference with the reference condition.

    Techniques Used: Hybridization, Concentration Assay, Polymerase Chain Reaction

    8) Product Images from "Poly(A) RNAs Including Coding Proteins RNAs Occur in Plant Cajal Bodies"

    Article Title: Poly(A) RNAs Including Coding Proteins RNAs Occur in Plant Cajal Bodies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111780

    Double labelling of a mixture of four mRNAs (A, C) and distinct genes: cyclin B1 mRNA (D, F), peroxidase mRNA (G, I), cytokinin-specific binding protein mRNA (J, L) and pectin methylesterase mRNA (M, O), with Sm proteins (B, E, H, K, N) in Lupinus cells. The arrows indicate colocalisation of mRNA transcripts with CBs. A stronger signal was observed in the cytoplasm than in the nuclei after in situ hybridisation with the mixture of probes (A, C) and with a probe against cytokinin-specific binding protein mRNA (J, L). % indicates the percentage of nuclei showing the representative immunolocalisation pattern. Bar, 5 µm. N-nucleus, Nu- nucleolus, C- cytoplasm.
    Figure Legend Snippet: Double labelling of a mixture of four mRNAs (A, C) and distinct genes: cyclin B1 mRNA (D, F), peroxidase mRNA (G, I), cytokinin-specific binding protein mRNA (J, L) and pectin methylesterase mRNA (M, O), with Sm proteins (B, E, H, K, N) in Lupinus cells. The arrows indicate colocalisation of mRNA transcripts with CBs. A stronger signal was observed in the cytoplasm than in the nuclei after in situ hybridisation with the mixture of probes (A, C) and with a probe against cytokinin-specific binding protein mRNA (J, L). % indicates the percentage of nuclei showing the representative immunolocalisation pattern. Bar, 5 µm. N-nucleus, Nu- nucleolus, C- cytoplasm.

    Techniques Used: Binding Assay, In Situ, Hybridization

    Analysis of the fluorescence intensity resulting from in situ hybridisation of poly(A) RNA in the meristematic cells of lupines roots. There were significant differences in intensity (p
    Figure Legend Snippet: Analysis of the fluorescence intensity resulting from in situ hybridisation of poly(A) RNA in the meristematic cells of lupines roots. There were significant differences in intensity (p

    Techniques Used: Fluorescence, In Situ, Hybridization

    9) Product Images from "A novel GAA-repeat-expansion-based mouse model of Friedreich’s ataxia"

    Article Title: A novel GAA-repeat-expansion-based mouse model of Friedreich’s ataxia

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.018952

    Transgene copy number. (A) Two TaqMan copy-number assays were applied: Hs05092416-cn assay (ATX 416; represented in black) was designed to amplify a 106-bp fragment of FXN within intron 3, and Hs02407730-cn assay (ATX 730; represented in grey) was designed to amplify an 80-bp fragment of FXN within intron 1 and exon 2. Human mammary epithelial cell (HMEC) with copy numbers of two served as a calibrator. Error bars=s.d. n =2. (B) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin-labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8sR cells. YG8sR showed one hybridisation signal, indicating a single integration site of the FXN transgene containing one copy of the FXN gene. Scale bars: 10 μm.
    Figure Legend Snippet: Transgene copy number. (A) Two TaqMan copy-number assays were applied: Hs05092416-cn assay (ATX 416; represented in black) was designed to amplify a 106-bp fragment of FXN within intron 3, and Hs02407730-cn assay (ATX 730; represented in grey) was designed to amplify an 80-bp fragment of FXN within intron 1 and exon 2. Human mammary epithelial cell (HMEC) with copy numbers of two served as a calibrator. Error bars=s.d. n =2. (B) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin-labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8sR cells. YG8sR showed one hybridisation signal, indicating a single integration site of the FXN transgene containing one copy of the FXN gene. Scale bars: 10 μm.

    Techniques Used: Transgenic Assay, Fluorescence In Situ Hybridization, Staining, Hybridization

    10) Product Images from "Occipital foramina development involves localised regulation of mesenchyme proliferation and is independent of apoptosis"

    Article Title: Occipital foramina development involves localised regulation of mesenchyme proliferation and is independent of apoptosis

    Journal: Journal of Anatomy

    doi: 10.1111/joa.12304

    In situ hybridisation of Sox9 during cranial foramina development. A diagram of cranial foramina in transverse section at early stages of development is shown in (A). Sox9 expression in the mesenchyme immediately surrounding the jugular blood vessel (jbv)
    Figure Legend Snippet: In situ hybridisation of Sox9 during cranial foramina development. A diagram of cranial foramina in transverse section at early stages of development is shown in (A). Sox9 expression in the mesenchyme immediately surrounding the jugular blood vessel (jbv)

    Techniques Used: In Situ, Hybridization, Expressing

    11) Product Images from "Poly(A) RNAs Including Coding Proteins RNAs Occur in Plant Cajal Bodies"

    Article Title: Poly(A) RNAs Including Coding Proteins RNAs Occur in Plant Cajal Bodies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111780

    Double labelling of a mixture of four mRNAs (A, C) and distinct genes: cyclin B1 mRNA (D, F), peroxidase mRNA (G, I), cytokinin-specific binding protein mRNA (J, L) and pectin methylesterase mRNA (M, O), with Sm proteins (B, E, H, K, N) in Lupinus cells. The arrows indicate colocalisation of mRNA transcripts with CBs. A stronger signal was observed in the cytoplasm than in the nuclei after in situ hybridisation with the mixture of probes (A, C) and with a probe against cytokinin-specific binding protein mRNA (J, L). % indicates the percentage of nuclei showing the representative immunolocalisation pattern. Bar, 5 µm. N-nucleus, Nu- nucleolus, C- cytoplasm.
    Figure Legend Snippet: Double labelling of a mixture of four mRNAs (A, C) and distinct genes: cyclin B1 mRNA (D, F), peroxidase mRNA (G, I), cytokinin-specific binding protein mRNA (J, L) and pectin methylesterase mRNA (M, O), with Sm proteins (B, E, H, K, N) in Lupinus cells. The arrows indicate colocalisation of mRNA transcripts with CBs. A stronger signal was observed in the cytoplasm than in the nuclei after in situ hybridisation with the mixture of probes (A, C) and with a probe against cytokinin-specific binding protein mRNA (J, L). % indicates the percentage of nuclei showing the representative immunolocalisation pattern. Bar, 5 µm. N-nucleus, Nu- nucleolus, C- cytoplasm.

    Techniques Used: Binding Assay, In Situ, Hybridization

    Analysis of the fluorescence intensity resulting from in situ hybridisation of poly(A) RNA in the meristematic cells of lupines roots. There were significant differences in intensity (p
    Figure Legend Snippet: Analysis of the fluorescence intensity resulting from in situ hybridisation of poly(A) RNA in the meristematic cells of lupines roots. There were significant differences in intensity (p

    Techniques Used: Fluorescence, In Situ, Hybridization

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    Whole Genome Amplification:

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    Ligation:

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    Purification:

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    Microarray:

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    Incubation:

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    Polymerase Chain Reaction:

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    Chromatin Immunoprecipitation:

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    Hybridization:

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    Article Snippet: .. Secondly, an alternative method was used for amplifying the DIP sample prior to microarray hybridisation using a proprietary whole genome amplification method (WGA2, Sigma-Aldrich) instead of ligation-mediated PCR. .. This advance in the application of microarray-based technology now offers a novel method and protocol for examining DNA damage in human and other cells.

    Article Title: Expression profiling of genes regulated by TGF-beta: Differential regulation in normal and tumour cells
    Article Snippet: .. 160 μl of hybridisation buffer (Ultrahyb, Sigma-Aldrich, USA) was added to the probe, incubated at 75°C for 5 minutes and then added to the human 19 K array (University Health network, Toronto). .. The hybridisation was carried out in the GeneTAC Hyb Station (Genomic Solutions, UK) at 65° for 4 hours, 60° for 4 hours and 55° for 10 hours.

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  • 99
    Millipore hybridization buffer
    Hybridization Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybridization buffer/product/Millipore
    Average 99 stars, based on 183 article reviews
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    hybridization buffer - by Bioz Stars, 2020-07
    99/100 stars
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