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GE Healthcare hybond h
Hybond H, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybond h/product/GE Healthcare
Average 82 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hybond h - by Bioz Stars, 2020-01
82/100 stars

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Agarose Gel Electrophoresis:

Article Title: Prevalence, distribution, and sequence diversity of hmwA among commensal and otitis media non-typeable Haemophilus influenzae
Article Snippet: .. A subset of hmwA -leader positive isolates ( ) were subjected to Southern blotting for detection of hmwA as follows: five micrograms of NTHi genomic DNA was digested with either Bgl II or Eco RI, separated on a 0.8% agarose gel in 1× TAE, and transferred by capillary blotting onto Hybond-H+ (Amersham Biosciences, Piscataway, NJ). ..

Southern Blot:

Article Title: Prevalence, distribution, and sequence diversity of hmwA among commensal and otitis media non-typeable Haemophilus influenzae
Article Snippet: .. A subset of hmwA -leader positive isolates ( ) were subjected to Southern blotting for detection of hmwA as follows: five micrograms of NTHi genomic DNA was digested with either Bgl II or Eco RI, separated on a 0.8% agarose gel in 1× TAE, and transferred by capillary blotting onto Hybond-H+ (Amersham Biosciences, Piscataway, NJ). ..

Northern Blot:

Article Title: Analysis of novel human papillomavirus type 16 late mRNAs in differentiated W12 cervical epithelial cells
Article Snippet: .. Northern blotting 10 μg total RNA or 0.5 μg polyadenylated RNA was fractionated on a denaturing 1.2% agarose–2.2 M formaldehyde gel and Northern blotted onto Hybond-H (Amersham) as described ( ). .. Blots were hybridised with [32 P]-labelled DNA probes synthesised by random priming in 5× SSC (1× SSC is 0.15 M sodium citrate)–50% formamide at 55 °C for 16 h and washed to 0.1× SSC at 65 °C followed by autoradiography.

Autoradiography:

Article Title: Analysis of novel human papillomavirus type 16 late mRNAs in differentiated W12 cervical epithelial cells
Article Snippet: Northern blotting 10 μg total RNA or 0.5 μg polyadenylated RNA was fractionated on a denaturing 1.2% agarose–2.2 M formaldehyde gel and Northern blotted onto Hybond-H (Amersham) as described ( ). .. Blots were hybridised with [32 P]-labelled DNA probes synthesised by random priming in 5× SSC (1× SSC is 0.15 M sodium citrate)–50% formamide at 55 °C for 16 h and washed to 0.1× SSC at 65 °C followed by autoradiography.

Blocking Assay:

Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
Article Snippet: Five hundred nanograms of DNA were dotted on a Hybond-H+ (Amersham Bioscience) membrane and cross-linked with 70,000 μJ cm−2 UV. .. The membrane was blocked with Odyssey blocking buffer (LI-COR Biosciences) with 1% SDS, incubated with IRDye Streptavidin 800CW, 1/10,000 (LI-COR Biosciences) in the same buffer, washed (three times in TBS-0.1% Tween20) and allowed to dry.

Incubation:

Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
Article Snippet: Five hundred nanograms of DNA were dotted on a Hybond-H+ (Amersham Bioscience) membrane and cross-linked with 70,000 μJ cm−2 UV. .. The membrane was blocked with Odyssey blocking buffer (LI-COR Biosciences) with 1% SDS, incubated with IRDye Streptavidin 800CW, 1/10,000 (LI-COR Biosciences) in the same buffer, washed (three times in TBS-0.1% Tween20) and allowed to dry.

Article Title: Prevalence, distribution, and sequence diversity of hmwA among commensal and otitis media non-typeable Haemophilus influenzae
Article Snippet: A subset of hmwA -leader positive isolates ( ) were subjected to Southern blotting for detection of hmwA as follows: five micrograms of NTHi genomic DNA was digested with either Bgl II or Eco RI, separated on a 0.8% agarose gel in 1× TAE, and transferred by capillary blotting onto Hybond-H+ (Amersham Biosciences, Piscataway, NJ). .. Briefly, the blot was incubated in 25 mls of 1× PerfectHyb Plus Hybridization buffer (Sigma-Aldrich, St. Louis, MO) for three hours at 68°C prior to overnight hybridization with the hmwA probe at 68°C in PerfectHyb Plus.

Modification:

Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
Article Snippet: Selective labelling of protein-blocked DNA ends The selective labelling of protein-blocked DNA ends (SLOPE) assay is a modification of selective labelling of TOP2 cleavage sites (SLOT) protocol . .. Five hundred nanograms of DNA were dotted on a Hybond-H+ (Amersham Bioscience) membrane and cross-linked with 70,000 μJ cm−2 UV.

Software:

Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
Article Snippet: Five hundred nanograms of DNA were dotted on a Hybond-H+ (Amersham Bioscience) membrane and cross-linked with 70,000 μJ cm−2 UV. .. Dots were analysed and quantified in Odyssey CLx with ImageStudio Odyssey CLx Software.

Plasmid Preparation:

Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
Article Snippet: Five hundred nanograms of DNA were dotted on a Hybond-H+ (Amersham Bioscience) membrane and cross-linked with 70,000 μJ cm−2 UV. .. The results are related to an internal control of the same labelling protocol performed on plasmid DNA.

Hybridization:

Article Title: Prevalence, distribution, and sequence diversity of hmwA among commensal and otitis media non-typeable Haemophilus influenzae
Article Snippet: A subset of hmwA -leader positive isolates ( ) were subjected to Southern blotting for detection of hmwA as follows: five micrograms of NTHi genomic DNA was digested with either Bgl II or Eco RI, separated on a 0.8% agarose gel in 1× TAE, and transferred by capillary blotting onto Hybond-H+ (Amersham Biosciences, Piscataway, NJ). .. Briefly, the blot was incubated in 25 mls of 1× PerfectHyb Plus Hybridization buffer (Sigma-Aldrich, St. Louis, MO) for three hours at 68°C prior to overnight hybridization with the hmwA probe at 68°C in PerfectHyb Plus.

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    GE Healthcare hybond h
    Hybond H, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybond h/product/GE Healthcare
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hybond h - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

    80
    GE Healthcare hybond h membrane
    Hybond H Membrane, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybond h membrane/product/GE Healthcare
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hybond h membrane - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    80
    GE Healthcare hybond h nylon membrane
    Hybond H Nylon Membrane, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybond h nylon membrane/product/GE Healthcare
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hybond h nylon membrane - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

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