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GE Healthcare hybond h
Hybond H, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybond h/product/GE Healthcare
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hybond h - by Bioz Stars, 2020-09
99/100 stars

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Agarose Gel Electrophoresis:

Article Title: Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen
Article Snippet: .. The DNA digests were separated on 0.8% agarose gel, then transferred to Hybond-N+ nylon membranes, according to the manufacturer's instructions (Amersham, USA). .. The membranes were blocked and hybridized with [32 p]cDNAlabeled probes prepared with a randomly primed DNA labeling kit (Boehringer Mannheim, Germany).

Northern Blot:

Article Title: GASTRIN-RELEASING PEPTIDE RECEPTOR IN BREAST CANCER MEDIATES CELLULAR MIGRATION AND INTERLEUKIN-8 EXPRESSION
Article Snippet: .. For Northern blot hybridization, 15 μg of total RNA was resolved on 1% agarose/formaldehyde gels by electrophoresis, transferred to Hybond=N+ membrane (Amersham Pharmacia Biotech, Buckinhamshire, England), aprobed with IL-8 cDNA labeled with [α-32 P] dATP (Perkin-Elmer Life Sciences Inc., Boston, MA) using a random-priming DNA-labeling kit (Stratagene, Cedar Creek, TX). .. Prehybridization (30 min) and hybridization (3 h) were performed at 68°C in Express Hybridization solution (Clontech, Palo Alto, CA).

Labeling:

Article Title: GASTRIN-RELEASING PEPTIDE RECEPTOR IN BREAST CANCER MEDIATES CELLULAR MIGRATION AND INTERLEUKIN-8 EXPRESSION
Article Snippet: .. For Northern blot hybridization, 15 μg of total RNA was resolved on 1% agarose/formaldehyde gels by electrophoresis, transferred to Hybond=N+ membrane (Amersham Pharmacia Biotech, Buckinhamshire, England), aprobed with IL-8 cDNA labeled with [α-32 P] dATP (Perkin-Elmer Life Sciences Inc., Boston, MA) using a random-priming DNA-labeling kit (Stratagene, Cedar Creek, TX). .. Prehybridization (30 min) and hybridization (3 h) were performed at 68°C in Express Hybridization solution (Clontech, Palo Alto, CA).

Electrophoresis:

Article Title: GASTRIN-RELEASING PEPTIDE RECEPTOR IN BREAST CANCER MEDIATES CELLULAR MIGRATION AND INTERLEUKIN-8 EXPRESSION
Article Snippet: .. For Northern blot hybridization, 15 μg of total RNA was resolved on 1% agarose/formaldehyde gels by electrophoresis, transferred to Hybond=N+ membrane (Amersham Pharmacia Biotech, Buckinhamshire, England), aprobed with IL-8 cDNA labeled with [α-32 P] dATP (Perkin-Elmer Life Sciences Inc., Boston, MA) using a random-priming DNA-labeling kit (Stratagene, Cedar Creek, TX). .. Prehybridization (30 min) and hybridization (3 h) were performed at 68°C in Express Hybridization solution (Clontech, Palo Alto, CA).

DNA Labeling:

Article Title: GASTRIN-RELEASING PEPTIDE RECEPTOR IN BREAST CANCER MEDIATES CELLULAR MIGRATION AND INTERLEUKIN-8 EXPRESSION
Article Snippet: .. For Northern blot hybridization, 15 μg of total RNA was resolved on 1% agarose/formaldehyde gels by electrophoresis, transferred to Hybond=N+ membrane (Amersham Pharmacia Biotech, Buckinhamshire, England), aprobed with IL-8 cDNA labeled with [α-32 P] dATP (Perkin-Elmer Life Sciences Inc., Boston, MA) using a random-priming DNA-labeling kit (Stratagene, Cedar Creek, TX). .. Prehybridization (30 min) and hybridization (3 h) were performed at 68°C in Express Hybridization solution (Clontech, Palo Alto, CA).

Western Blot:

Article Title: Utility of in vitro culture to the study of plant mitochondrial genome configuration and its dynamic features
Article Snippet: .. Total genomic DNA was digested with Bam HI (Arabidopsis) and Cla I (tobacco), and analyzed by DNA gel blot hybridization (Hybond-N, Amersham). .. Mitochondrial DNA repeats in Arabidopsis and tobacco mitochondrial genomes were PCR amplified, labeled with [α-32 P] dCTP by random priming (Stratagene), and used as probes.

Hybridization:

Article Title: Utility of in vitro culture to the study of plant mitochondrial genome configuration and its dynamic features
Article Snippet: .. Total genomic DNA was digested with Bam HI (Arabidopsis) and Cla I (tobacco), and analyzed by DNA gel blot hybridization (Hybond-N, Amersham). .. Mitochondrial DNA repeats in Arabidopsis and tobacco mitochondrial genomes were PCR amplified, labeled with [α-32 P] dCTP by random priming (Stratagene), and used as probes.

Article Title: GASTRIN-RELEASING PEPTIDE RECEPTOR IN BREAST CANCER MEDIATES CELLULAR MIGRATION AND INTERLEUKIN-8 EXPRESSION
Article Snippet: .. For Northern blot hybridization, 15 μg of total RNA was resolved on 1% agarose/formaldehyde gels by electrophoresis, transferred to Hybond=N+ membrane (Amersham Pharmacia Biotech, Buckinhamshire, England), aprobed with IL-8 cDNA labeled with [α-32 P] dATP (Perkin-Elmer Life Sciences Inc., Boston, MA) using a random-priming DNA-labeling kit (Stratagene, Cedar Creek, TX). .. Prehybridization (30 min) and hybridization (3 h) were performed at 68°C in Express Hybridization solution (Clontech, Palo Alto, CA).

SDS Page:

Article Title: M Phase Phosphoprotein 10 Is a Human U3 Small Nucleolar Ribonucleoprotein Component
Article Snippet: .. Proteins separated by SDS-PAGE were transferred to Immobilon-P or Hybond-N (Amersham, Arlington Heights, IL) and reacted with MPP10 antiserum at a dilution of 1:2000 to 1:10,000 in Tris-buffered saline containing Tween (50 mM Tris-HCl, pH 7.9, 150 mM NaCl, 0.1% Tween-20) with or without 5% nonfat dried milk. .. Bound antibodies were detected with horseradish peroxidase-conjugated anti-guinea pig antibody (Boehringer Mannheim, Indianapolis, IN, or Jackson ImmunoResearch Laboratories , West Grove, PA) at a dilution of 1:5000 and the ECL system (Amersham).

Staining:

Article Title: Characterization of an unusual tRNA-like sequence found inserted in a Neurospora retroplasmid
Article Snippet: .. After staining with ethidium bromide, the gel was blotted to Hybond-N (Amersham-Pharmacia) and the blots were hybridized overnight at 37°C with 32 P-labeled DNA probes ( > 5 × 104 c.p.m.) in 50% formamide, 5× SSC (1× SSC is 0.15 M NaCl, 0.015 M sodium citrate), 0.2% SDS and 100 mg/ml sheared, denatured salmon sperm DNA (Sigma). .. The blots were then washed in 5× SSC, 0.2% SDS for 5 min at 50°C and, if necessary to reduce background, once in 0.5× SSC, 0.2% SDS for an additional 5 min at 50°C, prior to autoradiography.

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    GE Healthcare hybond h membrane
    Hybond H Membrane, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybond h membrane/product/GE Healthcare
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    hybond h membrane - by Bioz Stars, 2020-09
    99/100 stars
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