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Journal: bioRxiv
Article Title: Metformin inhibits PDGF signaling to suppress hyaluronan and IL-6 production in Thyroid Eye Disease
doi: 10.1101/2025.05.19.654298
Figure Lengend Snippet: A) non-TED (n=3) and TED (n=3) OFs were treated with or without PDGFβ for 72 hours. Secreted HA levels in media supernatant collected after treatment were analyzed on agarose gel electrophoresis and stained using Stains-All. 15 MDa and HiLadder were used as a size standard. B) Quantitative HA levels in both non-TED and TED after treatment were measured as described in the Methods section. C) and D) The relative production of IL6 and IL8 by OFs after PDGFβ treatment for 72 hours was measured with ELISA assay. The experiments were conducted in triplicate. The data represent mean ± SEM and were analyzed statistically using One-way ANOVA with Tukey’s multiple comparisons. Vehicle versus PDGFβ: * P ≤ 0.05, ** P = 0.001, *** P < 0.0008, **** P < 0.0001, and non-TED versus TED: #### P ≤ 0.0001. HA, hyaluronan.
Article Snippet: 15 MDa HA (Lifecore Biomedical, Chaska, MN, HA15M-1) and
Techniques: Agarose Gel Electrophoresis, Staining, Enzyme-linked Immunosorbent Assay