Structured Review

TaKaRa human zyxin
Human Zyxin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human zyxin/product/TaKaRa
Average 83 stars, based on 2 article reviews
Price from $9.99 to $1999.99
human zyxin - by Bioz Stars, 2020-02
83/100 stars

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Related Articles

Clone Assay:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: To generate subcellular localization fusion vectors used for experiments in , the appropriate cloning vector and an mEmerald fusion vector were digested, either sequentially or doubly, with the appropriate enzymes and ligated together after gel purification. .. Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene).

Microscopy:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene). .. For FRET efficiency and lifetime microscopy experiments, the plasmid encoding the mCerulean:mVenus fusion was provided by Dr. Steven Vogel (NIH) , and was used to generate the mCerulean3:mVenus and mTurquoise:mVenus fusion proteins described in by substituting the coding sequence for mCerulean with the cDNA for mCerulean3.

Polymerase Chain Reaction:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: N3 and N1 constructs were generated by PCR using previously described methods . .. Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene).

Generated:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: N3 and N1 constructs were generated by PCR using previously described methods . .. Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene).

Construct:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: N3 and N1 constructs were generated by PCR using previously described methods . .. Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene).

Expressing:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: Paragraph title: Mammalian expression vectors ... Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene).

Sequencing:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene). .. For FRET efficiency and lifetime microscopy experiments, the plasmid encoding the mCerulean:mVenus fusion was provided by Dr. Steven Vogel (NIH) , and was used to generate the mCerulean3:mVenus and mTurquoise:mVenus fusion proteins described in by substituting the coding sequence for mCerulean with the cDNA for mCerulean3.

Plasmid Preparation:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: .. Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene). .. To prepare mCerulean3 C-terminal fusions, the following digests were performed: human β-actin, NheI and BglII (Clontech); human α-tubulin, NheI and BglII (Clontech); human light chain clathrin, NheI and BglII (George Patterson, NIH); human lamin B1, NheI and BglII (George Patterson, NIH); mouse MAP4, NheI and BglII (mouse microtubule associated protein 4, nucleotides 1918–3135, Richard Cyr, Penn State University); mouse light chain 9 myosin, NheI and BglII (Patricia Wadsworth, University of Massachusetts); human CDC-42, NheI and BglII (OriGene); PCNA, AgeI and BspEI (David Gilbert, FSU); mouse CAF-1, NheI and BglII (Akash Gunjan, FSU); human fibrillarin, AgeI and BspEI (Dimitry Chudakov, Russian Academy of Sciences); human GTPase Rab5a, NheI and BglII (Vicky Allen, University of Manchester).

Gel Purification:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: To generate subcellular localization fusion vectors used for experiments in , the appropriate cloning vector and an mEmerald fusion vector were digested, either sequentially or doubly, with the appropriate enzymes and ligated together after gel purification. .. Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene).

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  • 83
    TaKaRa human zyxin
    Human Zyxin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human zyxin/product/TaKaRa
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human zyxin - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    79
    TaKaRa human zyxin cdna
    Human Zyxin Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human zyxin cdna/product/TaKaRa
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human zyxin cdna - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    78
    TaKaRa full length human zyxin sequence
    Full Length Human Zyxin Sequence, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length human zyxin sequence/product/TaKaRa
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    full length human zyxin sequence - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

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