human sumo 1 c terminal (WuXi AppTec)

Structured Review

Human Sumo 1 C Terminal, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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1) Product Images from "Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei"
Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei
Journal: International Journal of Biological Sciences
doi:

Figure Legend Snippet: Nucleotide and deduced amino acid sequences of L. vannamei SUMO-1 (LvSUMO-1) cDNA. Amino acids are indicated as single capital letters under each triplet codon of the nucleotide sequence. The start codon is underlined and an asterisk (*) indicates the stop codon. Sumo modification prediction site on the polypeptide chain was showed in capital letters (AKPE). The polyadenylation signal (ATTAAA) is typed in capital letters underlined.
Techniques Used: Sequencing, Modification

Figure Legend Snippet: Amino acid sequence alignment among different SUMO-1 proteins. The amino acid sequences of Human (Accession no. AAH53528), Xenopus (Accession no. Q6DEP7), Zebrafish (Accession no. Q7SZR5), Brine shrimp: (Accession no. ABQ41279), Honeybee (Accession no. XP_392826), Wesp (Accession no. XP_001607301), Nematode (Accession no. CAP33470) and yeast (Accession no. NP_010798) are aligned. C-terminal diglycine motif, a site used for propeptide processing, is boxed, and the predicted cleavage site is marked by an arrowhead. A glutamine residue known to be important for the interaction of human SUMO-1 with protease Senp2 is asterisked.
Techniques Used: Sequencing

Figure Legend Snippet: Alignment of the LvSUMO-1 (sh1) with those of human SUMO-1 (hu1: GenBank Accession No. NP_003343), human SUMO-2(hu2: GenBank Accession No. P61956), human SUMO-3(hu: GenBank Accession No. NP_008867), mouse SUMO-1(m1: GenBank Accession No. NP_033486), mouse SUMO-2(m2: GenBank Accession No. NP_579932), mouse SUMO-3(m3: GenBank Accession No. Q9Z172) and Drosophilar smt3(dro: GenBank Accession No. NP_477411). Identical residues among all sequences are shown in black background and gaps are shown by hyphens. vanSUMO-1 grouped with human and mouse SUMO-1, is boxed.
Techniques Used:

Figure Legend Snippet: Western Blot analysis of shrimp SUMO-1 transcripts from various tissues compost of heart(H), intestine(I), hepatopancrease(Hep), haemocytes(Cy), lymphoid(L), brain and nerve(BN), Gill(G), pleopods(P) and abdominal muscle(AM) in cytosolic and nuclear protein fraction(M=protein marker). Protein expression level of shrimp SUMO-1 was very high expressed in hepatopancreas both in cytosilic and nuclear protein fraction. In addition, the expression in were found in heart and intestine of cytoplasmic protein fraction and in intestine and lymphoid of nuclear protein fraction.
Techniques Used: Western Blot, Marker, Expressing

Figure Legend Snippet: Differentiation stage of shrimp SUMO-1 gene expression by RT-PCR (Nau=nauplius, PL1= postlarvae 1 day, PL10= postlarvae 10 days, PL19= postlarvae 19 day and Adu= adult). The expression level is up regulated in all postlavae stated and adult.
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

Figure Legend Snippet: Shrimp SUMO-1 gene expression in different molting stage by RT-PCR. (A= postmolt, B=intermolt, C=premolt). SUMO-1 mRNA level is low expressed between postmolt and intermolt but high expressed in premolt.
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction