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human sumo 1 c terminal  (WuXi AppTec)


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    Structured Review

    WuXi AppTec human sumo 1 c terminal
    Nucleotide and deduced amino acid sequences of L. vannamei <t>SUMO-1</t> (LvSUMO-1) cDNA. Amino acids are indicated as single capital letters under each triplet codon of the nucleotide sequence. The start codon is underlined and an asterisk (*) indicates the stop codon. Sumo modification prediction site on the polypeptide chain was showed in capital letters (AKPE). The polyadenylation signal (ATTAAA) is typed in capital letters underlined.
    Human Sumo 1 C Terminal, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human sumo 1 c terminal/product/WuXi AppTec
    Average 86 stars, based on 1 article reviews
    human sumo 1 c terminal - by Bioz Stars, 2025-02
    86/100 stars

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    1) Product Images from "Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei"

    Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei

    Journal: International Journal of Biological Sciences

    doi:

    Nucleotide and deduced amino acid sequences of L. vannamei SUMO-1 (LvSUMO-1) cDNA. Amino acids are indicated as single capital letters under each triplet codon of the nucleotide sequence. The start codon is underlined and an asterisk (*) indicates the stop codon. Sumo modification prediction site on the polypeptide chain was showed in capital letters (AKPE). The polyadenylation signal (ATTAAA) is typed in capital letters underlined.
    Figure Legend Snippet: Nucleotide and deduced amino acid sequences of L. vannamei SUMO-1 (LvSUMO-1) cDNA. Amino acids are indicated as single capital letters under each triplet codon of the nucleotide sequence. The start codon is underlined and an asterisk (*) indicates the stop codon. Sumo modification prediction site on the polypeptide chain was showed in capital letters (AKPE). The polyadenylation signal (ATTAAA) is typed in capital letters underlined.

    Techniques Used: Sequencing, Modification

    Amino acid sequence alignment among different SUMO-1 proteins. The amino acid sequences of Human (Accession no. AAH53528), Xenopus (Accession no. Q6DEP7), Zebrafish (Accession no. Q7SZR5), Brine shrimp: (Accession no. ABQ41279), Honeybee (Accession no. XP_392826), Wesp (Accession no. XP_001607301), Nematode (Accession no. CAP33470) and yeast (Accession no. NP_010798) are aligned. C-terminal diglycine motif, a site used for propeptide processing, is boxed, and the predicted cleavage site is marked by an arrowhead. A glutamine residue known to be important for the interaction of human SUMO-1 with protease Senp2 is asterisked.
    Figure Legend Snippet: Amino acid sequence alignment among different SUMO-1 proteins. The amino acid sequences of Human (Accession no. AAH53528), Xenopus (Accession no. Q6DEP7), Zebrafish (Accession no. Q7SZR5), Brine shrimp: (Accession no. ABQ41279), Honeybee (Accession no. XP_392826), Wesp (Accession no. XP_001607301), Nematode (Accession no. CAP33470) and yeast (Accession no. NP_010798) are aligned. C-terminal diglycine motif, a site used for propeptide processing, is boxed, and the predicted cleavage site is marked by an arrowhead. A glutamine residue known to be important for the interaction of human SUMO-1 with protease Senp2 is asterisked.

    Techniques Used: Sequencing

    Alignment of the LvSUMO-1 (sh1) with those of human SUMO-1 (hu1: GenBank Accession No. NP_003343), human SUMO-2(hu2: GenBank Accession No. P61956), human SUMO-3(hu: GenBank Accession No. NP_008867), mouse SUMO-1(m1: GenBank Accession No. NP_033486), mouse SUMO-2(m2: GenBank Accession No. NP_579932), mouse SUMO-3(m3: GenBank Accession No. Q9Z172) and Drosophilar smt3(dro: GenBank Accession No. NP_477411). Identical residues among all sequences are shown in black background and gaps are shown by hyphens. vanSUMO-1 grouped with human and mouse SUMO-1, is boxed.
    Figure Legend Snippet: Alignment of the LvSUMO-1 (sh1) with those of human SUMO-1 (hu1: GenBank Accession No. NP_003343), human SUMO-2(hu2: GenBank Accession No. P61956), human SUMO-3(hu: GenBank Accession No. NP_008867), mouse SUMO-1(m1: GenBank Accession No. NP_033486), mouse SUMO-2(m2: GenBank Accession No. NP_579932), mouse SUMO-3(m3: GenBank Accession No. Q9Z172) and Drosophilar smt3(dro: GenBank Accession No. NP_477411). Identical residues among all sequences are shown in black background and gaps are shown by hyphens. vanSUMO-1 grouped with human and mouse SUMO-1, is boxed.

    Techniques Used:

    Western Blot analysis of shrimp SUMO-1 transcripts from various tissues compost of heart(H), intestine(I), hepatopancrease(Hep), haemocytes(Cy), lymphoid(L), brain and nerve(BN), Gill(G), pleopods(P) and abdominal muscle(AM) in cytosolic and nuclear protein fraction(M=protein marker). Protein expression level of shrimp SUMO-1 was very high expressed in hepatopancreas both in cytosilic and nuclear protein fraction. In addition, the expression in were found in heart and intestine of cytoplasmic protein fraction and in intestine and lymphoid of nuclear protein fraction.
    Figure Legend Snippet: Western Blot analysis of shrimp SUMO-1 transcripts from various tissues compost of heart(H), intestine(I), hepatopancrease(Hep), haemocytes(Cy), lymphoid(L), brain and nerve(BN), Gill(G), pleopods(P) and abdominal muscle(AM) in cytosolic and nuclear protein fraction(M=protein marker). Protein expression level of shrimp SUMO-1 was very high expressed in hepatopancreas both in cytosilic and nuclear protein fraction. In addition, the expression in were found in heart and intestine of cytoplasmic protein fraction and in intestine and lymphoid of nuclear protein fraction.

    Techniques Used: Western Blot, Marker, Expressing

    Differentiation stage of shrimp SUMO-1 gene expression by RT-PCR (Nau=nauplius, PL1= postlarvae 1 day, PL10= postlarvae 10 days, PL19= postlarvae 19 day and Adu= adult). The expression level is up regulated in all postlavae stated and adult.
    Figure Legend Snippet: Differentiation stage of shrimp SUMO-1 gene expression by RT-PCR (Nau=nauplius, PL1= postlarvae 1 day, PL10= postlarvae 10 days, PL19= postlarvae 19 day and Adu= adult). The expression level is up regulated in all postlavae stated and adult.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

    Shrimp SUMO-1 gene expression in different molting stage by RT-PCR. (A= postmolt, B=intermolt, C=premolt). SUMO-1 mRNA level is low expressed between postmolt and intermolt but high expressed in premolt.
    Figure Legend Snippet: Shrimp SUMO-1 gene expression in different molting stage by RT-PCR. (A= postmolt, B=intermolt, C=premolt). SUMO-1 mRNA level is low expressed between postmolt and intermolt but high expressed in premolt.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction



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    WuXi AppTec human sumo 1 c terminal
    Nucleotide and deduced amino acid sequences of L. vannamei <t>SUMO-1</t> (LvSUMO-1) cDNA. Amino acids are indicated as single capital letters under each triplet codon of the nucleotide sequence. The start codon is underlined and an asterisk (*) indicates the stop codon. Sumo modification prediction site on the polypeptide chain was showed in capital letters (AKPE). The polyadenylation signal (ATTAAA) is typed in capital letters underlined.
    Human Sumo 1 C Terminal, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human sumo 1 c terminal/product/WuXi AppTec
    Average 86 stars, based on 1 article reviews
    human sumo 1 c terminal - by Bioz Stars, 2025-02
    86/100 stars
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    Nucleotide and deduced amino acid sequences of L. vannamei SUMO-1 (LvSUMO-1) cDNA. Amino acids are indicated as single capital letters under each triplet codon of the nucleotide sequence. The start codon is underlined and an asterisk (*) indicates the stop codon. Sumo modification prediction site on the polypeptide chain was showed in capital letters (AKPE). The polyadenylation signal (ATTAAA) is typed in capital letters underlined.

    Journal: International Journal of Biological Sciences

    Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei

    doi:

    Figure Lengend Snippet: Nucleotide and deduced amino acid sequences of L. vannamei SUMO-1 (LvSUMO-1) cDNA. Amino acids are indicated as single capital letters under each triplet codon of the nucleotide sequence. The start codon is underlined and an asterisk (*) indicates the stop codon. Sumo modification prediction site on the polypeptide chain was showed in capital letters (AKPE). The polyadenylation signal (ATTAAA) is typed in capital letters underlined.

    Article Snippet: Proteins were transferred onto PVDF membrane (Bio Rad) in electoblotting buffer (25mM Tris-HCl, 190mM Glycine, 20% methanol) at a constant current of 2.5mA for 2 h. The membrane was immersed in blocking buffer (5% nonfat dry milk, 1X Tris buffer saline (TBS), pH 7.4, 0.1% Tween 20) at room temperature for 1 hr followed by incubation with rabbit polyclonal antibody to human SUMO-1 (c-terminal) (Abgent) in antibody dilution buffer (1% nonfat dry milk, 1x Tris buffer saline(TBS), pH 7.4, 0.1% Tween 20) at a concentration of 1:500 at 4 C overnight.

    Techniques: Sequencing, Modification

    Amino acid sequence alignment among different SUMO-1 proteins. The amino acid sequences of Human (Accession no. AAH53528), Xenopus (Accession no. Q6DEP7), Zebrafish (Accession no. Q7SZR5), Brine shrimp: (Accession no. ABQ41279), Honeybee (Accession no. XP_392826), Wesp (Accession no. XP_001607301), Nematode (Accession no. CAP33470) and yeast (Accession no. NP_010798) are aligned. C-terminal diglycine motif, a site used for propeptide processing, is boxed, and the predicted cleavage site is marked by an arrowhead. A glutamine residue known to be important for the interaction of human SUMO-1 with protease Senp2 is asterisked.

    Journal: International Journal of Biological Sciences

    Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei

    doi:

    Figure Lengend Snippet: Amino acid sequence alignment among different SUMO-1 proteins. The amino acid sequences of Human (Accession no. AAH53528), Xenopus (Accession no. Q6DEP7), Zebrafish (Accession no. Q7SZR5), Brine shrimp: (Accession no. ABQ41279), Honeybee (Accession no. XP_392826), Wesp (Accession no. XP_001607301), Nematode (Accession no. CAP33470) and yeast (Accession no. NP_010798) are aligned. C-terminal diglycine motif, a site used for propeptide processing, is boxed, and the predicted cleavage site is marked by an arrowhead. A glutamine residue known to be important for the interaction of human SUMO-1 with protease Senp2 is asterisked.

    Article Snippet: Proteins were transferred onto PVDF membrane (Bio Rad) in electoblotting buffer (25mM Tris-HCl, 190mM Glycine, 20% methanol) at a constant current of 2.5mA for 2 h. The membrane was immersed in blocking buffer (5% nonfat dry milk, 1X Tris buffer saline (TBS), pH 7.4, 0.1% Tween 20) at room temperature for 1 hr followed by incubation with rabbit polyclonal antibody to human SUMO-1 (c-terminal) (Abgent) in antibody dilution buffer (1% nonfat dry milk, 1x Tris buffer saline(TBS), pH 7.4, 0.1% Tween 20) at a concentration of 1:500 at 4 C overnight.

    Techniques: Sequencing

    Alignment of the LvSUMO-1 (sh1) with those of human SUMO-1 (hu1: GenBank Accession No. NP_003343), human SUMO-2(hu2: GenBank Accession No. P61956), human SUMO-3(hu: GenBank Accession No. NP_008867), mouse SUMO-1(m1: GenBank Accession No. NP_033486), mouse SUMO-2(m2: GenBank Accession No. NP_579932), mouse SUMO-3(m3: GenBank Accession No. Q9Z172) and Drosophilar smt3(dro: GenBank Accession No. NP_477411). Identical residues among all sequences are shown in black background and gaps are shown by hyphens. vanSUMO-1 grouped with human and mouse SUMO-1, is boxed.

    Journal: International Journal of Biological Sciences

    Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei

    doi:

    Figure Lengend Snippet: Alignment of the LvSUMO-1 (sh1) with those of human SUMO-1 (hu1: GenBank Accession No. NP_003343), human SUMO-2(hu2: GenBank Accession No. P61956), human SUMO-3(hu: GenBank Accession No. NP_008867), mouse SUMO-1(m1: GenBank Accession No. NP_033486), mouse SUMO-2(m2: GenBank Accession No. NP_579932), mouse SUMO-3(m3: GenBank Accession No. Q9Z172) and Drosophilar smt3(dro: GenBank Accession No. NP_477411). Identical residues among all sequences are shown in black background and gaps are shown by hyphens. vanSUMO-1 grouped with human and mouse SUMO-1, is boxed.

    Article Snippet: Proteins were transferred onto PVDF membrane (Bio Rad) in electoblotting buffer (25mM Tris-HCl, 190mM Glycine, 20% methanol) at a constant current of 2.5mA for 2 h. The membrane was immersed in blocking buffer (5% nonfat dry milk, 1X Tris buffer saline (TBS), pH 7.4, 0.1% Tween 20) at room temperature for 1 hr followed by incubation with rabbit polyclonal antibody to human SUMO-1 (c-terminal) (Abgent) in antibody dilution buffer (1% nonfat dry milk, 1x Tris buffer saline(TBS), pH 7.4, 0.1% Tween 20) at a concentration of 1:500 at 4 C overnight.

    Techniques:

    Western Blot analysis of shrimp SUMO-1 transcripts from various tissues compost of heart(H), intestine(I), hepatopancrease(Hep), haemocytes(Cy), lymphoid(L), brain and nerve(BN), Gill(G), pleopods(P) and abdominal muscle(AM) in cytosolic and nuclear protein fraction(M=protein marker). Protein expression level of shrimp SUMO-1 was very high expressed in hepatopancreas both in cytosilic and nuclear protein fraction. In addition, the expression in were found in heart and intestine of cytoplasmic protein fraction and in intestine and lymphoid of nuclear protein fraction.

    Journal: International Journal of Biological Sciences

    Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei

    doi:

    Figure Lengend Snippet: Western Blot analysis of shrimp SUMO-1 transcripts from various tissues compost of heart(H), intestine(I), hepatopancrease(Hep), haemocytes(Cy), lymphoid(L), brain and nerve(BN), Gill(G), pleopods(P) and abdominal muscle(AM) in cytosolic and nuclear protein fraction(M=protein marker). Protein expression level of shrimp SUMO-1 was very high expressed in hepatopancreas both in cytosilic and nuclear protein fraction. In addition, the expression in were found in heart and intestine of cytoplasmic protein fraction and in intestine and lymphoid of nuclear protein fraction.

    Article Snippet: Proteins were transferred onto PVDF membrane (Bio Rad) in electoblotting buffer (25mM Tris-HCl, 190mM Glycine, 20% methanol) at a constant current of 2.5mA for 2 h. The membrane was immersed in blocking buffer (5% nonfat dry milk, 1X Tris buffer saline (TBS), pH 7.4, 0.1% Tween 20) at room temperature for 1 hr followed by incubation with rabbit polyclonal antibody to human SUMO-1 (c-terminal) (Abgent) in antibody dilution buffer (1% nonfat dry milk, 1x Tris buffer saline(TBS), pH 7.4, 0.1% Tween 20) at a concentration of 1:500 at 4 C overnight.

    Techniques: Western Blot, Marker, Expressing

    Differentiation stage of shrimp SUMO-1 gene expression by RT-PCR (Nau=nauplius, PL1= postlarvae 1 day, PL10= postlarvae 10 days, PL19= postlarvae 19 day and Adu= adult). The expression level is up regulated in all postlavae stated and adult.

    Journal: International Journal of Biological Sciences

    Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei

    doi:

    Figure Lengend Snippet: Differentiation stage of shrimp SUMO-1 gene expression by RT-PCR (Nau=nauplius, PL1= postlarvae 1 day, PL10= postlarvae 10 days, PL19= postlarvae 19 day and Adu= adult). The expression level is up regulated in all postlavae stated and adult.

    Article Snippet: Proteins were transferred onto PVDF membrane (Bio Rad) in electoblotting buffer (25mM Tris-HCl, 190mM Glycine, 20% methanol) at a constant current of 2.5mA for 2 h. The membrane was immersed in blocking buffer (5% nonfat dry milk, 1X Tris buffer saline (TBS), pH 7.4, 0.1% Tween 20) at room temperature for 1 hr followed by incubation with rabbit polyclonal antibody to human SUMO-1 (c-terminal) (Abgent) in antibody dilution buffer (1% nonfat dry milk, 1x Tris buffer saline(TBS), pH 7.4, 0.1% Tween 20) at a concentration of 1:500 at 4 C overnight.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Shrimp SUMO-1 gene expression in different molting stage by RT-PCR. (A= postmolt, B=intermolt, C=premolt). SUMO-1 mRNA level is low expressed between postmolt and intermolt but high expressed in premolt.

    Journal: International Journal of Biological Sciences

    Article Title: Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei

    doi:

    Figure Lengend Snippet: Shrimp SUMO-1 gene expression in different molting stage by RT-PCR. (A= postmolt, B=intermolt, C=premolt). SUMO-1 mRNA level is low expressed between postmolt and intermolt but high expressed in premolt.

    Article Snippet: Proteins were transferred onto PVDF membrane (Bio Rad) in electoblotting buffer (25mM Tris-HCl, 190mM Glycine, 20% methanol) at a constant current of 2.5mA for 2 h. The membrane was immersed in blocking buffer (5% nonfat dry milk, 1X Tris buffer saline (TBS), pH 7.4, 0.1% Tween 20) at room temperature for 1 hr followed by incubation with rabbit polyclonal antibody to human SUMO-1 (c-terminal) (Abgent) in antibody dilution buffer (1% nonfat dry milk, 1x Tris buffer saline(TBS), pH 7.4, 0.1% Tween 20) at a concentration of 1:500 at 4 C overnight.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction