human serum  (Access Biologicals)


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    Name:
    Human Male AB Serum
    Description:
    Human Male AB is a proprietary product manufactured with either human or bovine thrombin following a cGMP process
    Catalog Number:
    515
    Price:
    None
    Category:
    Cell Culture product
    Size:
    100ml
    Buy from Supplier


    Structured Review

    Access Biologicals human serum
    Human Male AB is a proprietary product manufactured with either human or bovine thrombin following a cGMP process
    https://www.bioz.com/result/human serum/product/Access Biologicals
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human serum - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Modeling HIV-1 Latency Using Primary CD4+ T Cells from Virally Suppressed HIV-1-Infected Individuals on Antiretroviral Therapy
    Article Snippet: The purity of the enriched CD4+ T cells was greater than 94% based on flow cytometry. .. The CD4+ T cells were expanded in RPMI 1640 medium supplemented with 8% human serum (Access Biologicals), 1 μg/ml of phytohemagglutinin-L (PHA-L; Sigma-Aldrich), 10 ng/ml of rhIL-2 (IL-2 improved sequence; Miltenyi Biotec), 5 BRMP U/ml of natural human IL-2/TCGF (ZeptoMetrix), ARVs (100 nM efavirenz, 180 nM zidovudine, and 200 nM raltegravir), 100 U/ml of penicillin-streptomycin (Quality Biological), and irradiated feeder cells (10 × 106 cells/plate of freshly isolated PBMCs from three different HIV-negative donors and 1 × 106 cells/plate of an allogeneic Epstein-Barr virus immortalized B cell line) (T cell expansion media). .. The purified primary CD4+ T cells (up to 3 × 106 CD4+ T cells/plate), along with feeder cells, were suspended in 15 ml of the T cell expansion media and plated in 96-well U bottom plates (150 μl of the cell suspension/well).

    Article Title: CTL Clonotypes with Higher TCR Affinity Have Better Ability to Reduce the HIV Latent Reservoir.
    Article Snippet: The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction.

    Irradiation:

    Article Title: Modeling HIV-1 Latency Using Primary CD4+ T Cells from Virally Suppressed HIV-1-Infected Individuals on Antiretroviral Therapy
    Article Snippet: The purity of the enriched CD4+ T cells was greater than 94% based on flow cytometry. .. The CD4+ T cells were expanded in RPMI 1640 medium supplemented with 8% human serum (Access Biologicals), 1 μg/ml of phytohemagglutinin-L (PHA-L; Sigma-Aldrich), 10 ng/ml of rhIL-2 (IL-2 improved sequence; Miltenyi Biotec), 5 BRMP U/ml of natural human IL-2/TCGF (ZeptoMetrix), ARVs (100 nM efavirenz, 180 nM zidovudine, and 200 nM raltegravir), 100 U/ml of penicillin-streptomycin (Quality Biological), and irradiated feeder cells (10 × 106 cells/plate of freshly isolated PBMCs from three different HIV-negative donors and 1 × 106 cells/plate of an allogeneic Epstein-Barr virus immortalized B cell line) (T cell expansion media). .. The purified primary CD4+ T cells (up to 3 × 106 CD4+ T cells/plate), along with feeder cells, were suspended in 15 ml of the T cell expansion media and plated in 96-well U bottom plates (150 μl of the cell suspension/well).

    Article Title: CTL Clonotypes with Higher TCR Affinity Have Better Ability to Reduce the HIV Latent Reservoir.
    Article Snippet: The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction.

    Isolation:

    Article Title: Modeling HIV-1 Latency Using Primary CD4+ T Cells from Virally Suppressed HIV-1-Infected Individuals on Antiretroviral Therapy
    Article Snippet: The purity of the enriched CD4+ T cells was greater than 94% based on flow cytometry. .. The CD4+ T cells were expanded in RPMI 1640 medium supplemented with 8% human serum (Access Biologicals), 1 μg/ml of phytohemagglutinin-L (PHA-L; Sigma-Aldrich), 10 ng/ml of rhIL-2 (IL-2 improved sequence; Miltenyi Biotec), 5 BRMP U/ml of natural human IL-2/TCGF (ZeptoMetrix), ARVs (100 nM efavirenz, 180 nM zidovudine, and 200 nM raltegravir), 100 U/ml of penicillin-streptomycin (Quality Biological), and irradiated feeder cells (10 × 106 cells/plate of freshly isolated PBMCs from three different HIV-negative donors and 1 × 106 cells/plate of an allogeneic Epstein-Barr virus immortalized B cell line) (T cell expansion media). .. The purified primary CD4+ T cells (up to 3 × 106 CD4+ T cells/plate), along with feeder cells, were suspended in 15 ml of the T cell expansion media and plated in 96-well U bottom plates (150 μl of the cell suspension/well).

    Article Title: CTL Clonotypes with Higher TCR Affinity Have Better Ability to Reduce the HIV Latent Reservoir.
    Article Snippet: The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction.

    Staining:

    Article Title: CTL Clonotypes with Higher TCR Affinity Have Better Ability to Reduce the HIV Latent Reservoir.
    Article Snippet: The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction.

    Flow Cytometry:

    Article Title: CTL Clonotypes with Higher TCR Affinity Have Better Ability to Reduce the HIV Latent Reservoir.
    Article Snippet: The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction.

    Recombinant:

    Article Title: CTL Clonotypes with Higher TCR Affinity Have Better Ability to Reduce the HIV Latent Reservoir.
    Article Snippet: The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction. .. The success of the shock and kill strategy for the HIV cure depends both on the reactivation of the latent reservoir and on the ability of the immune system to eliminate infected cells.. As latency reversal alone has not shown any impact in the size of the latent reservoir, ensuring that effector CTLs are able to recognize and kill HIV-infected cells could contribute to reservoir reduction.

    In Vitro:

    Article Title: Preclinical Characterization of Radioimmunoconjugate 111In/90Y-FF-21101 Against P-cadherin Expressing Tumor in Mouse Xenograft Model and Non-Human Primate.
    Article Snippet: Kd and maximum binding (Bmax) values were calculated via nonlinear regression using GraphPad Prism Version 5.04 software (GraphPad Software, California, USA) [one site-binding analysis equation Y = Bmax × X / (Kd + X)]. .. In Vitro Stability in Serum 111In-FF-21101 and 90Y-FF-21101 were added to human serum (Access Biologicals, California, USA) at a concentration of 0.5 MBq/5 μg/mL (n=3). ..

    Concentration Assay:

    Article Title: Preclinical Characterization of Radioimmunoconjugate 111In/90Y-FF-21101 Against P-cadherin Expressing Tumor in Mouse Xenograft Model and Non-Human Primate.
    Article Snippet: Kd and maximum binding (Bmax) values were calculated via nonlinear regression using GraphPad Prism Version 5.04 software (GraphPad Software, California, USA) [one site-binding analysis equation Y = Bmax × X / (Kd + X)]. .. In Vitro Stability in Serum 111In-FF-21101 and 90Y-FF-21101 were added to human serum (Access Biologicals, California, USA) at a concentration of 0.5 MBq/5 μg/mL (n=3). ..

    Incubation:

    Article Title: C-terminal–modified LY2510924: a versatile scaffold for targeting C-X-C chemokine receptor type 4
    Article Snippet: The IC50 values were calculated by using GraphPad Prism version 5.04 (GraphPad Software, San Diego, CA). .. Stability assessment A 67Ga-FRM001 solution was diluted 11-fold in human serum (Access Biologicals, Vista, CA), and the solutions were then incubated at 37 °C. ..

    Proliferation Assay:

    Article Title: Pre-existing Immunity to Japanese Encephalitis Virus Alters CD4 T Cell Responses to Zika Virus Inactivated Vaccine
    Article Snippet: .. CD4 and CD8 T Cell Proliferation Assay PBMCs were labeled with CellTrace™ CFSE (Invitrogen, USA) following manufacturer's instructions, washed extensively, and cultured in RPMI supplemented with 8% human serum (Access Biologicals, USA) for 6 days in the presence of peptide pools (1 μg/mL each) or whole viral particle from ZPIV. .. For the negative control, each sample was also cultured with DMSO using the same volume as the peptide pools.

    Labeling:

    Article Title: Pre-existing Immunity to Japanese Encephalitis Virus Alters CD4 T Cell Responses to Zika Virus Inactivated Vaccine
    Article Snippet: .. CD4 and CD8 T Cell Proliferation Assay PBMCs were labeled with CellTrace™ CFSE (Invitrogen, USA) following manufacturer's instructions, washed extensively, and cultured in RPMI supplemented with 8% human serum (Access Biologicals, USA) for 6 days in the presence of peptide pools (1 μg/mL each) or whole viral particle from ZPIV. .. For the negative control, each sample was also cultured with DMSO using the same volume as the peptide pools.

    Cell Culture:

    Article Title: Pre-existing Immunity to Japanese Encephalitis Virus Alters CD4 T Cell Responses to Zika Virus Inactivated Vaccine
    Article Snippet: .. CD4 and CD8 T Cell Proliferation Assay PBMCs were labeled with CellTrace™ CFSE (Invitrogen, USA) following manufacturer's instructions, washed extensively, and cultured in RPMI supplemented with 8% human serum (Access Biologicals, USA) for 6 days in the presence of peptide pools (1 μg/mL each) or whole viral particle from ZPIV. .. For the negative control, each sample was also cultured with DMSO using the same volume as the peptide pools.

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