Structured Review

Becton Dickinson transient gfp endo transfection
Chloroquine treatment. (A and B) Fluorescence micrographs from mCCD cells transiently expressing <t>GFP-Endo</t> to label endocytic compartments. Typical punctuate fluorescent staining pattern of endosomes (green) in untreated cells (A) collapsed to distended coalesced structures (B) in chloroquine-treated cells (white bar = 5 μm, nuclei labeled blue). (C) Representative I SC trace from chloroquine-treated epithelium with repeated forskolin stimulation (gray bar). Note large dips in trace are the result of extended wash protocol to ensure total removal of forskolin from basal chamber. Addition of amiloride (black bar) following the third stimulation demonstrated that the majority of recorded I SC was due to ENaC.
Transient Gfp Endo Transfection, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Acute ENaC Stimulation by cAMP in a Kidney Cell Line is Mediated by Exocytic Insertion from a Recycling Channel Pool"

Article Title: Acute ENaC Stimulation by cAMP in a Kidney Cell Line is Mediated by Exocytic Insertion from a Recycling Channel Pool

Journal: The Journal of General Physiology

doi: 10.1085/jgp.200409124

Chloroquine treatment. (A and B) Fluorescence micrographs from mCCD cells transiently expressing GFP-Endo to label endocytic compartments. Typical punctuate fluorescent staining pattern of endosomes (green) in untreated cells (A) collapsed to distended coalesced structures (B) in chloroquine-treated cells (white bar = 5 μm, nuclei labeled blue). (C) Representative I SC trace from chloroquine-treated epithelium with repeated forskolin stimulation (gray bar). Note large dips in trace are the result of extended wash protocol to ensure total removal of forskolin from basal chamber. Addition of amiloride (black bar) following the third stimulation demonstrated that the majority of recorded I SC was due to ENaC.
Figure Legend Snippet: Chloroquine treatment. (A and B) Fluorescence micrographs from mCCD cells transiently expressing GFP-Endo to label endocytic compartments. Typical punctuate fluorescent staining pattern of endosomes (green) in untreated cells (A) collapsed to distended coalesced structures (B) in chloroquine-treated cells (white bar = 5 μm, nuclei labeled blue). (C) Representative I SC trace from chloroquine-treated epithelium with repeated forskolin stimulation (gray bar). Note large dips in trace are the result of extended wash protocol to ensure total removal of forskolin from basal chamber. Addition of amiloride (black bar) following the third stimulation demonstrated that the majority of recorded I SC was due to ENaC.

Techniques Used: Fluorescence, Expressing, Staining, Labeling

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