transient gfp endo transfection (Becton Dickinson)

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Becton Dickinson transient gfp endo transfection

Chloroquine treatment. (A and B) Fluorescence micrographs from mCCD cells transiently expressing <t>GFP-Endo</t> to label endocytic compartments. Typical punctuate fluorescent staining pattern of endosomes (green) in untreated cells (A) collapsed to distended coalesced structures (B) in chloroquine-treated cells (white bar = 5 μm, nuclei labeled blue). (C) Representative I SC trace from chloroquine-treated epithelium with repeated forskolin stimulation (gray bar). Note large dips in trace are the result of extended wash protocol to ensure total removal of forskolin from basal chamber. Addition of amiloride (black bar) following the third stimulation demonstrated that the majority of recorded I SC was due to ENaC.

Transient Gfp Endo Transfection, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transient gfp endo transfection/product/Becton Dickinson
Average 85 stars, based on 9 article reviews
Price from $9.99 to $1999.99

transient gfp endo transfection - by Bioz Stars, 2021-01

85/100 stars

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Images

1) Product Images from "Acute ENaC Stimulation by cAMP in a Kidney Cell Line is Mediated by Exocytic Insertion from a Recycling Channel Pool"

Article Title: Acute ENaC Stimulation by cAMP in a Kidney Cell Line is Mediated by Exocytic Insertion from a Recycling Channel Pool

Journal: The Journal of General Physiology

doi: 10.1085/jgp.200409124

Chloroquine treatment. (A and B) Fluorescence micrographs from mCCD cells transiently expressing GFP-Endo to label endocytic compartments. Typical punctuate fluorescent staining pattern of endosomes (green) in untreated cells (A) collapsed to distended coalesced structures (B) in chloroquine-treated cells (white bar = 5 μm, nuclei labeled blue). (C) Representative I SC trace from chloroquine-treated epithelium with repeated forskolin stimulation (gray bar). Note large dips in trace are the result of extended wash protocol to ensure total removal of forskolin from basal chamber. Addition of amiloride (black bar) following the third stimulation demonstrated that the majority of recorded I SC was due to ENaC.

Figure Legend Snippet: Chloroquine treatment. (A and B) Fluorescence micrographs from mCCD cells transiently expressing GFP-Endo to label endocytic compartments. Typical punctuate fluorescent staining pattern of endosomes (green) in untreated cells (A) collapsed to distended coalesced structures (B) in chloroquine-treated cells (white bar = 5 μm, nuclei labeled blue). (C) Representative I SC trace from chloroquine-treated epithelium with repeated forskolin stimulation (gray bar). Note large dips in trace are the result of extended wash protocol to ensure total removal of forskolin from basal chamber. Addition of amiloride (black bar) following the third stimulation demonstrated that the majority of recorded I SC was due to ENaC.

Techniques Used: Fluorescence, Expressing, Staining, Labeling

Staining:

Article Title: Succession of transiently active tumor‐initiating cell clones in human pancreatic cancer xenografts
Article Snippet: .. Primary pancreatic tumor cells were stained for 30 min on ice with antibodies against human CD44 (clone G44‐26, dilution 1:10, or 27–35, dilution 1:200; BD Biosciences), human CD24 (clone ML5, dilution 1:20; BD Biosciences), human EpCam (clone EBA‐1, dilution 1:20; BD Biosciences), human CD133 (clone AC133 and 293C3, dilution 1:10; Miltenyi), human thy1 (clone 5E10, dilution 1:200; BD Biosciences), and murine thy1.2 (clone 53‐2.1, dilution 1:50; BD Biosciences). ..

Recombinant:

Article Title: Matrix metalloproteinases cleave membrane-bound PD-L1 on CD90+ (myo-)fibroblasts in Crohn’s disease and regulate Th1/Th17 cell responses
Article Snippet: .. Antibodies, recombinant protein and chemicals Fluorochrome-conjugated forms of murine IgG1κ, isotype control and monoclonal antibodies (mAbs) directed against human CD90 (clone 5E10) were purchased from BD PharMingen (San Diego, CA, USA). .. Fluorochrome-conjugated mAbs against human PD-L1 (clone MIH1), PD-L2 (clone MIH18), human Fc receptor blocker and functional grade mouse IgG1κ isotype control were purchased from Thermo Fisher-eBioscience (San Diego, CA, USA).