human recombinant epidermal growth factor  (Thermo Fisher)


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    Name:
    EGF Recombinant Human Protein
    Description:
    Epidermal growth factor EGF recombinant human protein is supplied as a lyophilized powder It is suitable for use in performing cell based assays It can also be used as a protein standard or in other research applications This recombinant protein was expressed from a DNA sequence encoding the mature soluble form of human EGF NP 001954 2 Asn 971 Arg 1023 fused to the Fc region of human IgG1 at the N terminus Activity Measured in a cell proliferation assay using BALB c 3T3 mouse embryonic fibroblasts The EC50 for this effect is typically 20 80 pg ml Formulation Lyophilized in 140 mM NaCl 2 7 mM KCl 10 mM Na2HPO4 1 8 mM KH2PO4 pH 7 4 5 Mannitol 5 Trehalose 0 02 Tween 80 Reconstitution Dissolve the protein in sterile double distilled water to a concentration of 0 2 mg ml or lower It is recommended that the protein be aliquoted and be used as soon as possible Store aliquots under sterile conditions at 20°C Avoid repeated freeze thaw cycles Expiration Date Expires one year from date of receipt when stored as instructed This protein is manufactured by Sino Biological Inc
    Catalog Number:
    10605h01h25
    Price:
    None
    Applications:
    Enzyme & Protein Activity Assays|Protein Assay Controls, Reference Standards & Accessories|Protein Assays and Analysis|Protein Biology
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher human recombinant epidermal growth factor
    Epidermal growth factor EGF recombinant human protein is supplied as a lyophilized powder It is suitable for use in performing cell based assays It can also be used as a protein standard or in other research applications This recombinant protein was expressed from a DNA sequence encoding the mature soluble form of human EGF NP 001954 2 Asn 971 Arg 1023 fused to the Fc region of human IgG1 at the N terminus Activity Measured in a cell proliferation assay using BALB c 3T3 mouse embryonic fibroblasts The EC50 for this effect is typically 20 80 pg ml Formulation Lyophilized in 140 mM NaCl 2 7 mM KCl 10 mM Na2HPO4 1 8 mM KH2PO4 pH 7 4 5 Mannitol 5 Trehalose 0 02 Tween 80 Reconstitution Dissolve the protein in sterile double distilled water to a concentration of 0 2 mg ml or lower It is recommended that the protein be aliquoted and be used as soon as possible Store aliquots under sterile conditions at 20°C Avoid repeated freeze thaw cycles Expiration Date Expires one year from date of receipt when stored as instructed This protein is manufactured by Sino Biological Inc
    https://www.bioz.com/result/human recombinant epidermal growth factor/product/Thermo Fisher
    Average 99 stars, based on 394 article reviews
    Price from $9.99 to $1999.99
    human recombinant epidermal growth factor - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Induction of membrane-type-1 matrix metalloproteinase by epidermal growth factor-mediated signaling in gliomas 1
    Article Snippet: .. Glioma cells were treated with recombinant human EGF as described, prior to RNA extraction using Trizol Reagent, according to the manufacturer’s protocol (Invitrogen, Gaithersburg, Md.). ..

    Binding Assay:

    Article Title: Mammalian Expression and In Situ Biotinylation of Extracellular Protein Targets for Directed Evolution
    Article Snippet: .. To measure the binding of EGFR with its ligand, human recombinant EGF (Gibco Fisher, PHG0313) was adsorbed to an ELISA plate overnight at 4 °C in PBS. .. Following blocking with 2% (w/v) BSA in TBS, EGFR-ECD was serially diluted and allowed to bind.

    Cell Culture:

    Article Title: STAT3 Modulation to Enhance Motor Neuron Differentiation in Human Neural Stem Cells
    Article Snippet: .. Svendsen as we previously described , were cultured as free-floating “neurospheres” in 75-cm2 flasks with growth medium containing DMEM (high glucose, L-glutamine)/Ham’s F12 (3∶1; Invitrogen/Gibco, Grand Island, NY), and supplemented with 15 mM HEPES (Sigma, St. Louis, MO), 1.5% D-glucose (Sigma), 67 IU/ml/67 µg/ml penicillin/streptomycin (Mediatech, Inc/CellGro, Manassas, VA), 25 µg/ml bovine insulin (Sigma), 100 µg/ml human transferrin (Sigma), 100 µM putrescine (Sigma), 20 nM progesterone (Sigma), 30 nM sodium selenite (Sigma), 20 ng/ml recombinant human EGF (Invitrogen), 20 ng/ml recombinant human FGF2 (R & D Systems, Minneapolis, MN), 5 µg/ml heparin (R & D Systems), 10 ng/ml recombinant human LIF (Millipore), and 2 mM L-glutamine (Sigma). .. Cultured spheres were pelleted by centrifugation at 216×g for 10 min, resuspended in 0.025% trypsin (Sigma) plus 1.5% D-glucose dissolved in calcium- and magnesium-free Dulbecco’s phosphate buffered saline (CMF-dPBS, CellGro), and incubated for 10 min at 37°C with periodic trituration.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mammalian Expression and In Situ Biotinylation of Extracellular Protein Targets for Directed Evolution
    Article Snippet: .. To measure the binding of EGFR with its ligand, human recombinant EGF (Gibco Fisher, PHG0313) was adsorbed to an ELISA plate overnight at 4 °C in PBS. .. Following blocking with 2% (w/v) BSA in TBS, EGFR-ECD was serially diluted and allowed to bind.

    Labeling:

    Article Title: Endocytic Adaptor Protein HIP1R Controls Intracellular Trafficking of Epidermal Growth Factor Receptor in Neuronal Dendritic Development
    Article Snippet: .. Recombinant human EGF (0.5 μg/μl) was labeled with Alexa Fluor 647 Oligonucleotide Amine Labeling Kits (A20196, Invitrogen, 1 μg/μl) according to manufacturer’s instruction. .. Neurons at DIV 8 with or without HIP1R knockdown were incubated with 200 ng/ml Alexa-647-conjugated EGF (Alexa647 -EGF) for 20 min to allow for internalization.

    Recombinant:

    Article Title: STAT3 Modulation to Enhance Motor Neuron Differentiation in Human Neural Stem Cells
    Article Snippet: .. Svendsen as we previously described , were cultured as free-floating “neurospheres” in 75-cm2 flasks with growth medium containing DMEM (high glucose, L-glutamine)/Ham’s F12 (3∶1; Invitrogen/Gibco, Grand Island, NY), and supplemented with 15 mM HEPES (Sigma, St. Louis, MO), 1.5% D-glucose (Sigma), 67 IU/ml/67 µg/ml penicillin/streptomycin (Mediatech, Inc/CellGro, Manassas, VA), 25 µg/ml bovine insulin (Sigma), 100 µg/ml human transferrin (Sigma), 100 µM putrescine (Sigma), 20 nM progesterone (Sigma), 30 nM sodium selenite (Sigma), 20 ng/ml recombinant human EGF (Invitrogen), 20 ng/ml recombinant human FGF2 (R & D Systems, Minneapolis, MN), 5 µg/ml heparin (R & D Systems), 10 ng/ml recombinant human LIF (Millipore), and 2 mM L-glutamine (Sigma). .. Cultured spheres were pelleted by centrifugation at 216×g for 10 min, resuspended in 0.025% trypsin (Sigma) plus 1.5% D-glucose dissolved in calcium- and magnesium-free Dulbecco’s phosphate buffered saline (CMF-dPBS, CellGro), and incubated for 10 min at 37°C with periodic trituration.

    Article Title: Epidermal growth factor promotes cyclin G2 degradation via calpain-mediated proteolysis in gynaecological cancer cells
    Article Snippet: .. Recombinant human EGF and V5 antibody were from Life Technologies (Burlington, ON, Canada). .. Anti-p-Tyr, anti-GAPDH and anti-β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and used to detect non-specific phosphorylated tyrosine residues.

    Article Title: USP17 is required for clathrin mediated endocytosis of epidermal growth factor receptor
    Article Snippet: .. Cells were then stimulated with 0.32 nM-3.2 nM EGF Alexa Fluor 555 (Invitrogen-Molecular Probes, Eugene, USA) or recombinant human EGF (Invitrogen-Gibco, Maryland, USA) for the indicated times in the figures. .. EGF internalisation Transfected cells were rested for 3hrs in DMEM medium without serum and stimulated with 0.32nM-3.2nM EGF Alexa Fluor 555 (Invitrogen-Molecular Probes, Eugene, USA) or recombinant EGF (Invitrogen-Gibco, Maryland, USA) for the indicated times in the figures.

    Article Title: KRASG12C inhibition produces a driver-limited state revealing collateral dependencies
    Article Snippet: .. Recombinant human EGF and FGF2 (FGF-basic) were obtained from Thermo Fisher Scientific. .. TAMRA-N3 and tris(benzyltriazolylmethyl)amine (TBTA) were obtained from Click Chemistry Tools.

    Article Title: Endocytic Adaptor Protein HIP1R Controls Intracellular Trafficking of Epidermal Growth Factor Receptor in Neuronal Dendritic Development
    Article Snippet: .. Recombinant human EGF (0.5 μg/μl) was labeled with Alexa Fluor 647 Oligonucleotide Amine Labeling Kits (A20196, Invitrogen, 1 μg/μl) according to manufacturer’s instruction. .. Neurons at DIV 8 with or without HIP1R knockdown were incubated with 200 ng/ml Alexa-647-conjugated EGF (Alexa647 -EGF) for 20 min to allow for internalization.

    Article Title: The matrix protein CCN1 (CYR61) induces apoptosis in fibroblasts
    Article Snippet: .. Recombinant human EGF and basic FGF were obtained from Invitrogen. .. DRB, human VN, monoclonal anti–β-actin antibody (AC-15), and rabbit and mouse IgGs were purchased from Sigma-Aldrich.

    Article Title: Induction of membrane-type-1 matrix metalloproteinase by epidermal growth factor-mediated signaling in gliomas 1
    Article Snippet: .. Glioma cells were treated with recombinant human EGF as described, prior to RNA extraction using Trizol Reagent, according to the manufacturer’s protocol (Invitrogen, Gaithersburg, Md.). ..

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    Thermo Fisher human recombinant epidermal growth factor
    Human Recombinant Epidermal Growth Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant epidermal growth factor/product/Thermo Fisher
    Average 99 stars, based on 394 article reviews
    Price from $9.99 to $1999.99
    human recombinant epidermal growth factor - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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