Journal: International Journal of Molecular Sciences

Article Title: Association Between Pentraxins and Obesity in Prediabetes and Newly Diagnosed Type 2 Diabetes Mellitus Patients

doi: 10.3390/ijms26083661

Figure Lengend Snippet: Figure 1. The PTX3 (a), hs-CRP (b), TNF–α (c), IL–6 (d), PLR (e), AISI (f), MCVL (g), and IIC (h) levels for patients with preDM and T2DM vary in the HbA1c quartiles. When we compared the

Article Snippet: For each of the following mediators, we used commercially available test sets: PTX3 (Catalog# E-EL-H6081; sensitivity: 4.12 pg/mL; detection range: 6.86–5000 pg/mL), hs-CRP (Catalog# E-EL-H5134; sensitivity: 9.38 pg/mL; detection range: 15.63–1000 pg/mL), from the Elabscience (Houston, TX, USA); TNF-α (Catalog# BMS223-4; sensitivity: 2.3 pg/mL; assay range: 7.8–500 pg/mL), IL-6 (Catalog# BMS213-2; sensitivity: 0.92 pg/mL; assay range: 1.56–100 pg/mL), from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Association Between Pentraxins and Obesity in Prediabetes and Newly Diagnosed Type 2 Diabetes Mellitus Patients

doi: 10.3390/ijms26083661

Figure Lengend Snippet: Figure 4. Receiver operating characteristic (ROC) curve for IL-6 (A), PTX3 (B), MCVL (C), TNF-α (D), hs-CRP (E), PLR (F), SII (G), NLR (H), MLR (I), IIC (J), and AISI (K). ROC analysis showed that the PTX3 (AUC was 0.720; p = 0.003; cut-off value 1888.00 pg/mL, with 67.60% sensitivity and the highest specificity of 73.30%) and MCVL index (AUC was 0.677; p = 0.047; cut-off value 39.60, with 63.30% sensitivity and the second highest specificity of 66.70%) had a good accurate diagnosis compared with IL-6 (AUC was 0.866; p < 0.0001; cut-off value 40.30 pg/mL, with 100.00% sensitivity and 60.00% specificity). IIC showed 61.70% sensitivity and 60.00% specificity, with an AUC of 0.572, p = 0.027, and a cut-off value of 2.35. In our study, we found the following results for various hematological indices: PLR demonstrated 73.30% sensitivity and 60.00% specificity (cut-off value of 101); SII exhibited 56.70% sensitivity and 60.00% specificity (cut-off value of 397); NLR showed a sensitivity of 65.00% and a specificity of 60.00% (cut-off value of 1.98); MLR had a sensitivity of 53.30% and a specificity of 56.70% (cut-off value of 0.212); while AISI demonstrated a sensitivity of 56.70% and a specificity of 53.30% (cut-off value of 233). PTX3: pentraxin 3; hs-CRP: high-sensitivity C-reactive protein; TNF-α: tumor necrosis factor alpha; IL-6: interleukin 6; NLR: neutrophil-lymphocyte ratio; MLR: monocyte-lymphocyte ratio; PLR: platelet-lymphocyte ratio; AISI: aggregate index of systemic inflammation; SII: systemic immune-inflammation index; MCVL: ratio between the mean corpuscular volume/lymphocytes; IIC: cumulative inflammatory index.

Article Snippet: For each of the following mediators, we used commercially available test sets: PTX3 (Catalog# E-EL-H6081; sensitivity: 4.12 pg/mL; detection range: 6.86–5000 pg/mL), hs-CRP (Catalog# E-EL-H5134; sensitivity: 9.38 pg/mL; detection range: 15.63–1000 pg/mL), from the Elabscience (Houston, TX, USA); TNF-α (Catalog# BMS223-4; sensitivity: 2.3 pg/mL; assay range: 7.8–500 pg/mL), IL-6 (Catalog# BMS213-2; sensitivity: 0.92 pg/mL; assay range: 1.56–100 pg/mL), from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Techniques: Biomarker Discovery