human prostate cancer cell line  (LGC Standards)

Journal: Oncology Research

Article Title: TMED3 promotes prostate cancer via FOXO1a and FOXO3a phosphorylation

doi: 10.32604/or.2024.048054

Figure Lengend Snippet: The silencing efficiency of shTMED3. (A) DU145 cells were treated with shTMED3-1, shTMED3-2, and shTMED3-3 for 72 h and subjected to qRT-PCR. Relative TMED3 mRNA expression in DU145 was analyzed. (B) DU145 cells were treated with shTMED3-3 for 72 h and subjected to western blotting. The TMED3 protein level in DU145 was determined. * p < 0.05; ** p < 0.01.

Article Snippet: The human prostate cancer cell line DU145 without mycoplasma was obtained from Procell Life Science & Technology Co., Ltd. DU145 cells were cultured using DMEM with 10% FBS, penicillin and streptomycin under 5% CO 2 at 37 °C.

Techniques: Quantitative RT-PCR, Expressing, Western Blot

Journal: Oncology Research

Article Title: TMED3 promotes prostate cancer via FOXO1a and FOXO3a phosphorylation

doi: 10.32604/or.2024.048054

Figure Lengend Snippet: TMED3 regulated proliferation and promoted apoptosis in DU145 cells. (A) TMED3 protein levels in normal human prostate cells and prostate cancer cells were determined by western blotting. DU145 cells were infected with a lentivirus to silence TMED3 expression, and shCtrl was used as a scrambled control. (B) Cytotoxicity at different time points was determined by performing the CCK-8 assay. (C) A representative scatter plot of PI + Annexin V + and PI − Annexin V + DU145 cells by flow cytometry. (D) The proportions of DU145 cells at different stages of apoptosis (early apoptosis, late apoptosis, and total apoptosis) by flow cytometry. (E and F) The cell cycle (G1, S, and G2 phases) of DU145 cells was determined by flow cytometry. ** p < 0.01; *** p < 0.001.

Article Snippet: The human prostate cancer cell line DU145 without mycoplasma was obtained from Procell Life Science & Technology Co., Ltd. DU145 cells were cultured using DMEM with 10% FBS, penicillin and streptomycin under 5% CO 2 at 37 °C.

Techniques: Western Blot, Infection, Expressing, Control, CCK-8 Assay, Flow Cytometry

Journal: Oncology Research

Article Title: TMED3 promotes prostate cancer via FOXO1a and FOXO3a phosphorylation

doi: 10.32604/or.2024.048054

Figure Lengend Snippet: TMED3 regulated the invasion and migration of prostate cancer cells. DU145 cells were infected with a lentivirus to silence TMED3 expression, and shCtrl was used as a scrambled control. (A and B) The wound-healing assay was performed to determine the invasion ability of DU145 cells. (C and D) DU145 cell migration was assessed by performing the Transwell assay. *** p < 0.001. Original magnification, 200×.

Article Snippet: The human prostate cancer cell line DU145 without mycoplasma was obtained from Procell Life Science & Technology Co., Ltd. DU145 cells were cultured using DMEM with 10% FBS, penicillin and streptomycin under 5% CO 2 at 37 °C.

Techniques: Migration, Infection, Expressing, Control, Wound Healing Assay, Transwell Assay

Journal: Oncology Research

Article Title: TMED3 promotes prostate cancer via FOXO1a and FOXO3a phosphorylation

doi: 10.32604/or.2024.048054

Figure Lengend Snippet: TMED3 inhibited the FOXO pathway by inducing FOXO1a and FOXO3a phosphorylation during prostate cancer progression. (A) The enrichment analysis of the TCGA data was performed to analyze the correlation of TMED3 with 26 signaling pathways. The sizes of the circles indicate the significance of enrichment, whereas their colors represent the strength and direction of the enrichment (red: positive correlation, blue: negative correlation). (B) The key role of the FOXO family in cancer-related pathways. (C) Western blotting of FOXO1a, p-FOXO1a, FOXO3a, and p-FOXO3a in DU145 cells.

Article Snippet: The human prostate cancer cell line DU145 without mycoplasma was obtained from Procell Life Science & Technology Co., Ltd. DU145 cells were cultured using DMEM with 10% FBS, penicillin and streptomycin under 5% CO 2 at 37 °C.

Techniques: Western Blot