human polyclonal glyceraldehyde 3 phosphate dehydrogenase gapdh  (Millipore)


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    Structured Review

    Millipore human polyclonal glyceraldehyde 3 phosphate dehydrogenase gapdh
    Human Polyclonal Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human polyclonal glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human polyclonal glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    93/100 stars

    Related Products / Commonly Used Together

    mouse monoclonal antibodies against β-actin

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    Western Blot:

    Article Title: Electrophilic fatty acid nitroalkenes regulate Nrf2 and NF-κB signaling:A medicinal chemistry investigation of structure-function relationships
    Article Snippet: Paragraph title: Western blotting ... To verify protein loading, membranes were subsequently stripped and reprobed with mouse monoclonal antibodies against β-actin (Sigma) as well as the human polyclonal glyceraldehyde 3-phosphate dehydrogenase (gapdh) purchased from Sigma (A4700) and Trevigen (2275), respectively.

    Incubation:

    Article Title: Electrophilic fatty acid nitroalkenes regulate Nrf2 and NF-κB signaling:A medicinal chemistry investigation of structure-function relationships
    Article Snippet: Membranes were probed with antibodies to inducible nitric oxide synthase (iNOS) at 1:1500 (Cell Signaling; 13120), heme oxygenase1 (HO1) at 1:5000 (Enzo Life; ADI-SPA-896), NAD(P)H dehydrogenase quinone 1 (NQO1) at 1:1500 (Abcam, ab34173), glutamate–cysteine ligase complex modifier subunit (GCLM) at 1:1000 (Invitrogen, 50–55–466) overnight, washed with TTBS, and then incubated with horseradish peroxidase-conjugated antibodies at 1:10,000 dilution. .. To verify protein loading, membranes were subsequently stripped and reprobed with mouse monoclonal antibodies against β-actin (Sigma) as well as the human polyclonal glyceraldehyde 3-phosphate dehydrogenase (gapdh) purchased from Sigma (A4700) and Trevigen (2275), respectively.

    Stripping Membranes:

    Article Title: Electrophilic fatty acid nitroalkenes regulate Nrf2 and NF-κB signaling:A medicinal chemistry investigation of structure-function relationships
    Article Snippet: Control experiments were performed to ensure that there were no immunoreactive bands following the stripping protocol for GCLM and NQO1. .. To verify protein loading, membranes were subsequently stripped and reprobed with mouse monoclonal antibodies against β-actin (Sigma) as well as the human polyclonal glyceraldehyde 3-phosphate dehydrogenase (gapdh) purchased from Sigma (A4700) and Trevigen (2275), respectively.

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  • 90
    Millipore rabbit anti bax
    Effects of coffee oil-algae oil nanoemulsions on protein expressions of <t>cyclin</t> B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and <t>Bax,</t> Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p
    Rabbit Anti Bax, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax/product/Millipore
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rabbit anti bax - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    82
    Millipore rabbit anti bax antibody
    Relationship of BNIP3 expression to <t>Bax</t> activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control <t>COX</t> IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.
    Rabbit Anti Bax Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax antibody/product/Millipore
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti bax antibody - by Bioz Stars, 2020-04
    82/100 stars
      Buy from Supplier

    Image Search Results


    Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Journal: International Journal of Nanomedicine

    Article Title: Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

    doi: 10.2147/IJN.S144705

    Figure Lengend Snippet: Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Article Snippet: The primary antibodies include mouse monoclonal anti-α-tubulin antibody (Sigma–Aldrich Co.), mouse anti-cyclin A and rabbit anti-Bax (EMD Millipore), mouse anti-CDK2, mouse anti-cytochrome C, mouse anti-p21, mouse anti-cyclin B, and mouse anti-Bcl-2 (BD Biosciences), as well as anti-cdc2 (CDK1) and mouse anti-p53 (Novus Biologicals Co., Littleton, CO, USA).

    Techniques: Incubation, Standard Deviation

    L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Journal: BMC Medicine

    Article Title: Sodium vanadate combined with l-ascorbic acid delays disease progression, enhances motor performance, and ameliorates muscle atrophy and weakness in mice with spinal muscular atrophy

    doi: 10.1186/1741-7015-11-38

    Figure Lengend Snippet: L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Article Snippet: Primary antibodies used for western blotting included mouse anti-SMN (1:5,000; BD Biosciences, San Diego, CA, USA), mouse anti-β-actin (1:10,000; Sigma), rabbit anti-Bax (1:1,000; Millipore, Temecula, CA, USA), and rabbit anti-caspase 3 (1:1,000; Cell Signaling, Temecula, CA, USA).

    Techniques: Western Blot, Expressing

    Scheme of AUR/BSO- or AUR/ERA-triggered synergistic cell death. AUR inhibits TrxR, which leads to upregulation of GSH, inhibiting AUR. ERA or BSO cause depletion of GSH levels, thereby counteracting the AUR-mediated upregulation of GSH and increasing AUR's cytotoxic activity. This leads to proteasome inhibition and subsequently to accumulation of ubiquitinated NOXA and MCL-1, followed by activation of BAX/BAK and caspases. See text for more details

    Journal: Cell Death & Disease

    Article Title: Targeting redox homeostasis in rhabdomyosarcoma cells: GSH-depleting agents enhance auranofin-induced cell death

    doi: 10.1038/cddis.2017.412

    Figure Lengend Snippet: Scheme of AUR/BSO- or AUR/ERA-triggered synergistic cell death. AUR inhibits TrxR, which leads to upregulation of GSH, inhibiting AUR. ERA or BSO cause depletion of GSH levels, thereby counteracting the AUR-mediated upregulation of GSH and increasing AUR's cytotoxic activity. This leads to proteasome inhibition and subsequently to accumulation of ubiquitinated NOXA and MCL-1, followed by activation of BAX/BAK and caspases. See text for more details

    Article Snippet: Western blot analysis Western blot analysis was performed as described previously, using the following antibodies: mouse anti-GPX4 (R & D Systems, Wiesbaden, Germany), rabbit anti-NQO1 and mouse IgG1 anti-ubiquitin (P4D1) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-BAX (Millipore, Darmstadt, Germany), mouse anti-NOXA (Alexis Biochemicals, Grünberg, Germany), rabbit anti-BAK (BD Biosciences), mouse anti-PARP (Cell Signaling, Beverly, MA, USA), rabbit anti-MCL-1 (Stressgen, Victoria, BC, Canada), mouse anti-GAPDH (HyTest, Turku, Finland) or mouse anti-β -Actin (Sigma-Aldrich).

    Techniques: Activity Assay, Inhibition, Activation Assay

    Relationship of BNIP3 expression to Bax activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control COX IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: Cyanide-Induced Apoptosis of Dopaminergic Cells Is Promoted by BNIP3 and Bax Modulation of Endoplasmic Reticulum-Mitochondrial Ca2+ Levels S⃞

    doi: 10.1124/jpet.109.159103

    Figure Lengend Snippet: Relationship of BNIP3 expression to Bax activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control COX IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.

    Article Snippet: The primary antibodies were: mouse anti-BNIP3 antibody, mouse anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO), mouse anti-cytochrome c oxidase (COX IV), rabbit anticalnexin antibodies (Abcam Inc., Cambridge, MA), rabbit anti-Bax antibody (Millipore, Billerica, MA), and mouse anti-Bax antibody (Santa Cruz Biotechnology, San Cruz, CA).

    Techniques: Expressing, Activation Assay, Transfection, Western Blot, Plasmid Preparation