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blinded human plasma  (Bio-Rad)
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Bio-Rad blinded human plasma
Blinded Human Plasma, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human plasma fibronectin  (Thermo Fisher)
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Thermo Fisher human plasma fibronectin
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Human Plasma Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human plasma fibronectin - by Bioz Stars, 2024-12
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normal human plasma  (Sysmex Corporation)
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Sysmex Corporation normal human plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Normal Human Plasma, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human plasma/product/Sysmex Corporation
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normal human plasma - by Bioz Stars, 2024-12
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incubatedwith healthy human plasma  (Danaher Inc)
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Danaher Inc incubatedwith healthy human plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Incubatedwith Healthy Human Plasma, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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incubatedwith healthy human plasma - by Bioz Stars, 2024-12
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human plasma  (Danaher Inc)
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Danaher Inc human plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Human Plasma, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human plasma/product/Danaher Inc
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human plasma - by Bioz Stars, 2024-12
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human plasma  (Millipore)
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Millipore human plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Human Plasma, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human plasma/product/Millipore
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human plasma - by Bioz Stars, 2024-12
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charcoal stripped human plasma  (BioIVT Inc)
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BioIVT Inc charcoal stripped human plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Charcoal Stripped Human Plasma, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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charcoal stripped human plasma - by Bioz Stars, 2024-12
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human plasma  (Cosmo Bio USA)
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Cosmo Bio USA human plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Human Plasma, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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technology nist standardized human plasma  (National Institute of Standards and Technology)
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National Institute of Standards and Technology technology nist standardized human plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Technology Nist Standardized Human Plasma, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human k2edta plasma  (Keystone Bioanalytical)
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Keystone Bioanalytical human k2edta plasma
Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or <t>fibronectin.</t> At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.
Human K2edta Plasma, supplied by Keystone Bioanalytical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or fibronectin. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.

Journal: APL Bioengineering

Article Title: 3D confinement alters smooth muscle cell responses to chemical and mechanical cues

doi: 10.1063/5.0225569

Figure Lengend Snippet: Cell–ECM and cell–cell interactions regulate SMC gene expression and contractility in confinement. (a) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m channels coated with collagen I or fibronectin. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05. No statistically significant difference was observed between 4- μ m channel with fibronectin and 4- μ m channel with collagen I and between 4- μ m channel with fibronectin and 10- μ m channel with either fibronectin or collagen-I. Statistical comparisons are made using two-way ANOVA. (b) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I or fibronectin after 4 days of entrapment. At least 12 cells analyzed per experiment; 3 independent experiments. Data are mean ± S.D. ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (c) Schematic showing coating of a surface with CDH11-FC or CDH2-FC. (d) Quantification of CArG-RE activity on day 4 (normalized to day 0) in SMCs entrapped in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.E.M. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA. (e) Percent decrease in SMC length in response to 0.25 μ M U-46619 in 10- or 4- μ m microchannels coated with collagen I, CDH11-FC, or CDH2-FC after 4 days of entrapment. At least 12 cells analyzed per experiment; at least 3 independent experiments. Data are mean ± S.D. * p < 0.05, ** p < 0.01. Statistical comparisons are made using two-way ANOVA.

Article Snippet: Microchannels were then coated with the following ECM molecules: rat tail collagen I (20 μ g/mL; Gibco), collagen I-FITC (20 μ g/mL; Millipore-Sigma), and fibronectin-FITC (40 μ g/mL; Millipore-Sigma) for 1 h at 37 °C, laminin (20 μ g/mL; SouthernBiotech) for 1 h at 37 °C and then overnight at 4 °C, and human plasma fibronectin (20 μ g/mL; Gibco) overnight at 4 °C.

Techniques: Expressing, Activity Assay