human peripheral blood mononuclear cells pbmcs  (R&D Systems)

 
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    R&D Systems human peripheral blood mononuclear cells pbmcs
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmcs/product/R&D Systems
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    91/100 stars

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    Recombinant:

    Article Title: Inhibition of TGF-? Enhances the In Vivo Antitumor Efficacy of EGF Receptor-Targeted Therapy
    Article Snippet: .. Human peripheral blood mononuclear cells (PBMC) from normal donors were stimulated with recombinant human interleukin-2 (rh IL-2; 200 IU/mL; Chiron) in the presence or absence of rhTGFβ1 (5 ng/mL; R & D Systems) in Adoptive Immunotherapy Media V (AIM V) containing low Ig FBS (Invitrogen) for 48 hours and used as effectors. .. Tumor cells (5,000 cells/well) were cultured in a 96-well U-bottomed plate in growth medium containing low Ig serum for 24 hours before being used as targets.

    other:

    Article Title: The Canarypox-virus vaccine vector ALVAC triggers the release of IFN-γ by Natural Killer (NK) cells enhancing Th1 polarization
    Article Snippet: 2.10 Cell death analysis Cell death analysis of either human peripheral blood mononuclear cells (PBMCs) or murine BMDCs was carried out using the TACS Annexin-V-FITC Apoptosis Detection Kit as described by the manufacturer (R & D Systems).

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    R&D Systems alloreactive human pbmc response
    FTY720 suppressed <t>Alloreactive</t> T-cell responses in mice and human with reducing pro-inflammatory cytokines. a , b In the mixed lymphocyte reaction assay, a total of 10 5 B/c(B6) splenic T cells (responders) were incubated with 10 5 irradiated B/c(B6) (syngeneic stimulators [Syn]) or B6(B/c) (allogeneic stimulators [Allo]) splenic APCs for 4 days. c In the mixed lymphocyte reaction assay, human <t>PBMC</t> of healthy volunteer were incubated with another volunteer for 4 days. d IL-17 and IL-10 concentrations in culture supernatants as determined by ELISA
    Alloreactive Human Pbmc Response, supplied by R&D Systems, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alloreactive human pbmc response/product/R&D Systems
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    R&D Systems dendritic cells pbmc dc
    Production of cytokines in human <t>PBMC/DCs</t> co-cultured with recombinant Lactobacillus Human Peripheral Blood Mononuclear Cells (PBMCs) were treated with 100 nM GM-CSF and 10 nM IL-4 during 5 days to derive monocytes into dendritic cells. 10 6 cells/well were seed in 24 well plates and co-cultured with UV-killed recombinant Lactobacillus expressing wild type LcrV (LpV) or ss LcrV (Lp ss V) at MOI 10:1 colony-forming units per cell. 100 ng/ml Escherichia coli O111:B4 lipopolysaccharide (LPS) and L. plantarum (Lp) were used as positive and negative control, respectively. After 48 h of stimulation, supernatants were collected and TNFα (A), IL-12 (B), IFNγ (C), IL-6 (D), and IL-10 (E) cytokine production was measured by sandwich ELISA (Quantikine). The average of triplicate samples was determined and the error bar indicates standard deviation. Results are representative of one of three independent experiments. * p =0.001, ** p =0.0001, *** p =0.05
    Dendritic Cells Pbmc Dc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems pbmc
    Mesenchymal stem or stromal cells (MSC) reduced the proliferation of <t>CD4</t> + T cells and suppressed tumour necrosis factor (TNF)-α production in vivo . Peripheral blood mononuclear cells <t>(PBMC)</t> labelled with 10 μM carboxyfluorescein succinimidyl
    Pbmc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc/product/R&D Systems
    Average 93 stars, based on 35 article reviews
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    FTY720 suppressed Alloreactive T-cell responses in mice and human with reducing pro-inflammatory cytokines. a , b In the mixed lymphocyte reaction assay, a total of 10 5 B/c(B6) splenic T cells (responders) were incubated with 10 5 irradiated B/c(B6) (syngeneic stimulators [Syn]) or B6(B/c) (allogeneic stimulators [Allo]) splenic APCs for 4 days. c In the mixed lymphocyte reaction assay, human PBMC of healthy volunteer were incubated with another volunteer for 4 days. d IL-17 and IL-10 concentrations in culture supernatants as determined by ELISA

    Journal: Journal of Translational Medicine

    Article Title: FTY720 ameliorates GvHD by blocking T lymphocyte migration to target organs and by skin fibrosis inhibition

    doi: 10.1186/s12967-020-02386-w

    Figure Lengend Snippet: FTY720 suppressed Alloreactive T-cell responses in mice and human with reducing pro-inflammatory cytokines. a , b In the mixed lymphocyte reaction assay, a total of 10 5 B/c(B6) splenic T cells (responders) were incubated with 10 5 irradiated B/c(B6) (syngeneic stimulators [Syn]) or B6(B/c) (allogeneic stimulators [Allo]) splenic APCs for 4 days. c In the mixed lymphocyte reaction assay, human PBMC of healthy volunteer were incubated with another volunteer for 4 days. d IL-17 and IL-10 concentrations in culture supernatants as determined by ELISA

    Article Snippet: Cytokine level measurement by enzyme-linked immunosorbent assay (ELISA)Concentrations of interleukin (IL)-17, and IL-10 in culture supernatants from alloreactive human PBMC response were measured using an ELISA DuoSet (R & D Systems, Lille, France).

    Techniques: Mouse Assay, Incubation, Irradiation, Enzyme-linked Immunosorbent Assay

    Production of cytokines in human PBMC/DCs co-cultured with recombinant Lactobacillus Human Peripheral Blood Mononuclear Cells (PBMCs) were treated with 100 nM GM-CSF and 10 nM IL-4 during 5 days to derive monocytes into dendritic cells. 10 6 cells/well were seed in 24 well plates and co-cultured with UV-killed recombinant Lactobacillus expressing wild type LcrV (LpV) or ss LcrV (Lp ss V) at MOI 10:1 colony-forming units per cell. 100 ng/ml Escherichia coli O111:B4 lipopolysaccharide (LPS) and L. plantarum (Lp) were used as positive and negative control, respectively. After 48 h of stimulation, supernatants were collected and TNFα (A), IL-12 (B), IFNγ (C), IL-6 (D), and IL-10 (E) cytokine production was measured by sandwich ELISA (Quantikine). The average of triplicate samples was determined and the error bar indicates standard deviation. Results are representative of one of three independent experiments. * p =0.001, ** p =0.0001, *** p =0.05

    Journal: Vaccine

    Article Title: Platform technology to deliver prophylactic molecules orally: an example using the Class A select agent Yersinia pestis

    doi: 10.1016/j.vaccine.2010.07.084

    Figure Lengend Snippet: Production of cytokines in human PBMC/DCs co-cultured with recombinant Lactobacillus Human Peripheral Blood Mononuclear Cells (PBMCs) were treated with 100 nM GM-CSF and 10 nM IL-4 during 5 days to derive monocytes into dendritic cells. 10 6 cells/well were seed in 24 well plates and co-cultured with UV-killed recombinant Lactobacillus expressing wild type LcrV (LpV) or ss LcrV (Lp ss V) at MOI 10:1 colony-forming units per cell. 100 ng/ml Escherichia coli O111:B4 lipopolysaccharide (LPS) and L. plantarum (Lp) were used as positive and negative control, respectively. After 48 h of stimulation, supernatants were collected and TNFα (A), IL-12 (B), IFNγ (C), IL-6 (D), and IL-10 (E) cytokine production was measured by sandwich ELISA (Quantikine). The average of triplicate samples was determined and the error bar indicates standard deviation. Results are representative of one of three independent experiments. * p =0.001, ** p =0.0001, *** p =0.05

    Article Snippet: To derive the monocyte population of the PBMCs into dendritic cells (PBMC/DC) we cultured 1×106 cells/well in 24-well tissue culture plates for 5 days in 2 ml of complete RPMI 1640 supplemented with 10 ng/ml IL-4, and 100 ng/ml recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) (R & D system, Minneapolis, MN).

    Techniques: Cell Culture, Recombinant, Expressing, Negative Control, Sandwich ELISA, Standard Deviation

    Mesenchymal stem or stromal cells (MSC) reduced the proliferation of CD4 + T cells and suppressed tumour necrosis factor (TNF)-α production in vivo . Peripheral blood mononuclear cells (PBMC) labelled with 10 μM carboxyfluorescein succinimidyl

    Journal: Clinical and Experimental Immunology

    Article Title: Human mesenchymal stem cells suppress donor CD4+ T cell proliferation and reduce pathology in a humanized mouse model of acute graft-versus-host disease

    doi: 10.1111/cei.12056

    Figure Lengend Snippet: Mesenchymal stem or stromal cells (MSC) reduced the proliferation of CD4 + T cells and suppressed tumour necrosis factor (TNF)-α production in vivo . Peripheral blood mononuclear cells (PBMC) labelled with 10 μM carboxyfluorescein succinimidyl

    Article Snippet: Human CD4+ T cells were isolated from PBMC by magnetic bead separation following the manufacturer's guidelines (R & D Systems, Minneapolis, MN, USA).

    Techniques: In Vivo

    Co-culture of stress-treated DC with autologous PBMC induce CD40L and CD45RO + memory in CD4 + T cells. These are significantly inhibited with both IL-15 antibodies and IL-1β antagonists with all stress agents. For optimum expression of human CD45RO

    Journal: The Journal of Biological Chemistry

    Article Title: Stress-activated Dendritic Cells (DC) Induce Dual Interleukin (IL)-15- and IL1β-mediated Pathways, Which May Elicit CD4+ Memory T Cells and Interferon (IFN)-stimulated Genes *

    doi: 10.1074/jbc.M115.645754

    Figure Lengend Snippet: Co-culture of stress-treated DC with autologous PBMC induce CD40L and CD45RO + memory in CD4 + T cells. These are significantly inhibited with both IL-15 antibodies and IL-1β antagonists with all stress agents. For optimum expression of human CD45RO

    Article Snippet: To ascertain whether the homeostasis (IL-15-mediated) or inflammasomes (IL-1β mediated) or both pathways are involved in eliciting CD40L and CD45RO+ memory T cells, co-cultures of DC and PBMC were treated with either 10 μg/ml of anti-IL-15 neutralizing antibodies (R & D Systems) or 50 μ m IL-1β receptor I antagonist (IL-1β RI, Merck) or both were used with previously determined optimum doses of inhibitors.

    Techniques: Co-Culture Assay, Expressing