Journal: Biological Research

Article Title: Identification and functional characteristics of CHD1L gene variants implicated in human Müllerian duct anomalies

doi: 10.1186/s40659-024-00550-w

Figure Lengend Snippet: The interaction between WT or mutant CHD1L and PARP1. A Co-IP assay performed using cell lysates of 293FT cells co-transfected with exogenous flag-tagged CHD1L and HA-tagged PARP1 plasmids. B Co-IP assay between exogenous flag-tagged CHD1L proteins and endogenous PARP1. ACTB was used as loading control. C Schematic representation illustrating the molecular docking of Macro domain of human WT CHD1L and PARP1

Article Snippet: The related primary antibodies included GAPDH antibody (AC033; ABclonal, China), DYKDDDDK tag antibody (66008-4-Ig; ProteinTech, China), HA tag antibody (51064-2-AP; ProteinTech, China), anti-β actin antibody (TA-09; ZSGB-BIO, China), anti-CHD1L antibody (ab197019; Abcam, USA) and human PARP1 antibody (13371-1-AP; ProteinTech, China).

Techniques: Mutagenesis, Co-Immunoprecipitation Assay, Transfection, Control

Journal: Biological Research

Article Title: Identification and functional characteristics of CHD1L gene variants implicated in human Müllerian duct anomalies

doi: 10.1186/s40659-024-00550-w

Figure Lengend Snippet: The recruitment of CHD1L to sites of laser-induced DNA damage. A The locations of WT or mutant GFP-CHD1L and mCherry-PARP1 in living 293FT cells under fluorescence microscope. The GFP-NC plasmid transfected was used as negative control. The cellular nucleus was stained using Hoechst (blue). B Mobilization, association, and dissociation of CHD1L to the specific sites of laser-induced DNA damage. C – E Quantification of the recruitment time, dissociation time and normalized fluorescence of WT and R319Q CHD1L proteins at DNA damage sites. Plotted data were presented as the mean ± SD of 25 cells. ****P < 0.0001, ***P < 0.001, unpaired, two-tailed Student’s t test

Article Snippet: The related primary antibodies included GAPDH antibody (AC033; ABclonal, China), DYKDDDDK tag antibody (66008-4-Ig; ProteinTech, China), HA tag antibody (51064-2-AP; ProteinTech, China), anti-β actin antibody (TA-09; ZSGB-BIO, China), anti-CHD1L antibody (ab197019; Abcam, USA) and human PARP1 antibody (13371-1-AP; ProteinTech, China).

Techniques: Mutagenesis, Fluorescence, Microscopy, Plasmid Preparation, Transfection, Negative Control, Staining, Two Tailed Test

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: PARP-1, EpCAM, and FRα as potential targets for intraoperative detection and delineation of endometriosis: a quantitative tissue expression analysis

doi: 10.1186/s12958-024-01264-0

Figure Lengend Snippet: PARP-1 expression in the cohort of patients. ( A ) Whole slide image with a zoomed-in cross sectional area showing the endometriosis lesion. ( B ) Representative microphotographs showing expression of PARP-1 by IHC in endometriosis lesions and surrounding tissues. DAB (brown) chromogen was used to visualize the binding of the anti-human PARP-1 antibody. Magnification 100x; scale bars, 50 μm. In representative images of the output from the quantification analyses, the red signal corresponds to the 3 + signal (labeled as strong positive), the orange signal corresponds to the 2 + signal (labeled as positive), the yellow signal corresponds to the 1 + signal (labeled as weak positive), and finally, the blue signal corresponds to the negative expression (labeled as negative signal). For the average percentage calculation, we considered only strong positive signals and normal positive signals, as shown and described in Additional file

Article Snippet: We used the following primary antibodies: rabbit anti-human PARP-1 (clone EPR18461, 1:100 pH6, Abcam), rabbit anti-human EpCAM (1:400 pH9, Abcam), rabbit anti-human FRα (1:1000 pH9, Thermofisher).

Techniques: Expressing, Binding Assay, Labeling

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: PARP-1, EpCAM, and FRα as potential targets for intraoperative detection and delineation of endometriosis: a quantitative tissue expression analysis

doi: 10.1186/s12958-024-01264-0

Figure Lengend Snippet: Comparison of the percentages of PARP-1, EpCAM, and FRα positivity between lesion samples and surrounding tissues (paired bootstrap t-test adjusted p-values < 10 − 5 ). ST: Surrounding tissue, Nsp: number of strong positive cells, Np: number of positive cells, Nt: number of total cells

Article Snippet: We used the following primary antibodies: rabbit anti-human PARP-1 (clone EPR18461, 1:100 pH6, Abcam), rabbit anti-human EpCAM (1:400 pH9, Abcam), rabbit anti-human FRα (1:1000 pH9, Thermofisher).

Techniques: Comparison

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: PARP-1, EpCAM, and FRα as potential targets for intraoperative detection and delineation of endometriosis: a quantitative tissue expression analysis

doi: 10.1186/s12958-024-01264-0

Figure Lengend Snippet: Representative microphotographs of double immunofluorescence staining for EpCAM, PARP-1, and FRα in endometriosis foci. The upper panels show EpCAM (green signal) and PARP-1 (red signal) expression. In the lower panels, FRα (green signal) and PARP-1 (red signal) are shown. Nuclei were stained with DAPI. Magnification 200x

Article Snippet: We used the following primary antibodies: rabbit anti-human PARP-1 (clone EPR18461, 1:100 pH6, Abcam), rabbit anti-human EpCAM (1:400 pH9, Abcam), rabbit anti-human FRα (1:1000 pH9, Thermofisher).

Techniques: Double Immunofluorescence Staining, Expressing, Staining

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: PARP1 expression in CRC and adjacent normal mucosal tissues. There was A positive and B weakly positive PARP1 expression in primary CRC (×200 magnification). There was negative PARP1 expression in primary CRC ( C ) and normal colorectal mucosa tissue ( D ). Western blotting ( E ) and real-time PCR ( F ). PARP1 expression was examined in eight CRC cell lines and in normal colonic epithelial cell (FHC) using Western blotting ( G ) and real-time PCR ( H ). ( p < 0.05; Fig. C, D). Blot menbranes were cut prior to hybridisation with antibodies during blotting and developed with X-ray film. The scale bar represents 100 µm. * p < 0.05, (Student’s t test)

Article Snippet: SW620 cells and anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA) were used.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Hybridization

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: Survival curve in regards to PARP1 expression and the PARP1 expression in SW480/SW620 cells. Patients with positive PARP1 expression have shorter OS ( A ) and RFS ( B ) than patients with negative PARP1 expression ( p = 0.001 ; p = 0.001 , respectively)

Article Snippet: SW620 cells and anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA) were used.

Techniques: Expressing

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: Clinicopathological characteristics and PARP1 expression status of patients with colorectal cancer

Article Snippet: SW620 cells and anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA) were used.

Techniques: Expressing

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: A Western blotting analysis of PARP1 expression in SW480/SW620-Vector, SW480/SW620-PARP1, SW480/SW620-Scramble, and SW480/SW620-PARP1/RNAi cells. B QPCR analysis of PARP1 mRNA expression in SW480 and SW620 cells. Blot menbranes were cut prior to hybridisation with antibodies during blotting and developed with X-ray film. *P < 0.05 , (Student’s t test)

Article Snippet: SW620 cells and anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA) were used.

Techniques: Western Blot, Expressing, Plasmid Preparation, Hybridization

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: Cell proliferation analysis. CCK-8 assay ( A ) and Colony formation assay ( B ) of SW480/SW620 cells transfected with PARP1, PARP1/RNAi, and Vector/Scramble. C Western blot analysis of cyclin D1 and p21 expression. D QPCR analysis of cyclin D1 and p21 expression. Blot membranes were cut prior to hybridisation with antibodies during blotting and developed with X-ray film. *p < 0.05 , (Student’s t test)

Article Snippet: SW620 cells and anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA) were used.

Techniques: CCK-8 Assay, Colony Assay, Transfection, Plasmid Preparation, Western Blot, Expressing, Hybridization

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: PARP1 interacted with XRCC2 regulates CRC cell proliferation. A The IP results of XRCC2 protein interacting with PARP1 protein. Input indicates the positive control group, IgG indicates the negative control group, IP indicates the target experimental group. B Western blot analysis of PARP1 expression. C CCK-8 assay and D colony formation assay to test cell proliferation of the PARP1 overexpression cells in SW480/SW620 transfected with NC, XRCC2/siRNA. Blot membranes were cut prior to hybridisation with antibodies during blotting and developed with X-ray film. *p < 0.05, (Student’s t test)

Article Snippet: SW620 cells and anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA) were used.

Techniques: Positive Control, Negative Control, Western Blot, Expressing, CCK-8 Assay, Colony Assay, Over Expression, Transfection, Hybridization

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: PARP1 expression in primary CRC tissue and adjacent noncancerous tissue

Article Snippet: SW620 cells and anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam, Cambridge, MA, USA) were used.

Techniques: Expressing