human nonsmall cell lung cancer cell line a549  (Danaher Inc)

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and A549 cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Staining, Expressing, Incubation, Blocking Assay, Fluorescence, Software, Irradiation

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a), (b) Cytotoxicity and phototoxicity of free CUR/ICG, CUR/ICG-LPs and GE11-CUR/ICG-LPs under the NIR laser irradiation (808 nm) of 1 W·cm −2 for 5 min at different concentrations on A549 cells after 24 h incubation. (c) Fluorescence images of A549 cells stained with calcein-AM (green) after different treatments. Scale bar = 100 μ m. (d) Synergistic effect of photo- and chemotherapy based on GE11-CUR/ICG-LPs. Cytotoxicity of GE11-ICG-LPs, GE11-CUR-LPs, and GE11-CUR/ICG-LPs with or without laser exposure was performed by MTT assays. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Irradiation, Incubation, Fluorescence, Staining

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a) Effects of IC-GLPs on the caspase-3 activity in A549 cells, data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001. (b) Intracellular ROS detection by the DCFH-DA fluorescence staining. Scale bar = 50 μ m. (c) Cytoskeletal microtubulin (red) expression in A549 cells after different treatments with laser irradiation. DAPI (blue) counterstains cell nuclei. Scale bar = 10 μ m.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Activity Assay, Fluorescence, Staining, Expressing, Irradiation

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a) Representative western blot and (b) quantification of Bcl-2 and Bax expression in A549 cells following treatment with PBS, free CUR/ICG, CUR/ICG-LPs, and GE11-CUR/ICG-LPs under 808 nm laser irradiation for 5 min. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01. (c) Representative western blot and (d) quantification of PI3K, p-PI3K, Akt, and p-Akt expression in A549 cells following different treatments with laser irradiation. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Western Blot, Expressing, Irradiation

Journal: Frontiers in Oncology

Article Title: RNA Demethylase ALKBH5 Prevents Lung Cancer Progression by Regulating EMT and Stemness via Regulating p53

doi: 10.3389/fonc.2022.858694

Figure Lengend Snippet: Enrichment of CSCs from A549 and PC-9 cells. (A) After being cultured in a serum-free medium, spheres derived from A549 and PC-9 were imaged at an amplification of ×10. (B) By performing CCK-8 assay, the cell viability of CSCs and their parental cells from days 1 to 5 was measured. * p < 0.05 vs. the parental cell group. The mRNA (C) and protein levels (D) of stemness hallmarkers, including CD24, CD44, ALDH1, Nanog, Oct4, and Sox2 were measured by performing RT-qPCR and Western blot analysis. * p < 0.05 vs. parental cell group.

Article Snippet: Nonsmall cell lung cancer (NSCLC) cell lines A549 and PC-9 were all purchased from the American Type Culture Collection (ATCC).

Techniques: Cell Culture, Derivative Assay, Amplification, CCK-8 Assay, Quantitative RT-PCR, Western Blot

Journal: Frontiers in Oncology

Article Title: RNA Demethylase ALKBH5 Prevents Lung Cancer Progression by Regulating EMT and Stemness via Regulating p53

doi: 10.3389/fonc.2022.858694

Figure Lengend Snippet: CSCs present a relatively lower m 6 A methylation level compared with their parental cells. (A) Global m 6 A methylation was measured in CSCs and its parental cells of A549 and PC-9. * p < 0.05 vs. parental cells. m 6 A methylation-relative gene expressions including METLL3, METLL14, YTHDF1, WTAP, FTO, and ALKBH5 were measured in mRNA (B) and protein levels (C) . (D) By employing the GEPIA database analysis tool, the relative mRNA levels of METLL3, METLL14, YTHDF1, WTAP, FTO, and ALKBH5 were compared between tumor samples and nontumor samples.

Article Snippet: Nonsmall cell lung cancer (NSCLC) cell lines A549 and PC-9 were all purchased from the American Type Culture Collection (ATCC).

Techniques: Methylation

Journal: Frontiers in Oncology

Article Title: RNA Demethylase ALKBH5 Prevents Lung Cancer Progression by Regulating EMT and Stemness via Regulating p53

doi: 10.3389/fonc.2022.858694

Figure Lengend Snippet: p53 transcriptionally regulates ALKBH5 and subsequently decreased m 6 A methylation. (A) GEPIA database analysis tool was used to compare the correlation between ALKBH5 and p53 in LUAD and LUSC. (B) In A549 and PC-9 CSCs, the efficiency of p53 knockdown or overexpression was measured by performing a Western blot analysis. (C) After modification of p53 with or without PFT-α, ALKBH5 mRNA and protein level were measured. * p < 0.05 vs. shScrambled group; # p < 0.05 vs. p53 group. (D) After modification of p53 with or without PFT-α, m 6 A methylation level was measured. * p < 0.05 vs. shScrambled group; # p < 0.05 vs. shp53 group (left panel); * p < 0.05, vs. vector group; # p < 0.05 vs. p53 group (right panel).

Article Snippet: Nonsmall cell lung cancer (NSCLC) cell lines A549 and PC-9 were all purchased from the American Type Culture Collection (ATCC).

Techniques: Methylation, Over Expression, Western Blot, Modification, Plasmid Preparation

Journal: Frontiers in Oncology

Article Title: RNA Demethylase ALKBH5 Prevents Lung Cancer Progression by Regulating EMT and Stemness via Regulating p53

doi: 10.3389/fonc.2022.858694

Figure Lengend Snippet: p53 inhibits malignancies via exerting transcriptional activity. In A549 CSCs, p53 was efficiently knockdown by transfecting shRNA targeting to p53 mRNA. In PC-9 CSCs, p53 was efficiently overexpressed by transfecting p53-coding plasmid. Cell viability was then analyzed by performing CCK-8 assay (A) . * p < 0.05 vs. shScrambled group (left panel); * p < 0.05 vs. vector group (right panel). (B) Cell cycle distribution was analyzed by performing PI staining followed by flow cytometry assay. * p < 0.05 vs. shScrambled group; # p < 0.05 vs. shp53 group (left panel); * p < 0.05 vs. vector group; # p < 0.05 vs. p53 group (right panel). (C) Cell invasion was analyzed by performing Transwell assay. * p <0.05 vs. shScrambled group; # p < 0.05, vs. shp53 group (left panel); * p < 0.05, vs. vector group; # p < 0.05 vs. p53 group (right panel). (D) Tumor formation in soft agar was performed. * p < 0.05 vs. shScrambled group; # p < 0.05 vs. shp53 group (left panel); * p < 0.05 vs. vector group; # p < 0.05 vs. p53 group (right panel).

Article Snippet: Nonsmall cell lung cancer (NSCLC) cell lines A549 and PC-9 were all purchased from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, shRNA, Plasmid Preparation, CCK-8 Assay, Staining, Flow Cytometry, Transwell Assay

Journal: Frontiers in Oncology

Article Title: RNA Demethylase ALKBH5 Prevents Lung Cancer Progression by Regulating EMT and Stemness via Regulating p53

doi: 10.3389/fonc.2022.858694

Figure Lengend Snippet: p53 potentially regulates PRRX1, Sox2, and E-cadherin via ALKBH5. In A549 CSCs, p53 was efficiently knockdown by transfecting shRNA targeting p53 mRNA. In PC-9 CSCs, p53 was efficiently overexpressed by transfecting p53-coding plasmid. mRNA (A) and protein (B) of PRRX1, Sox2, and E-cadherin were measured. * p < 0.05 shScrambled group (left panel); * p < 0.05 vs. vector group; # p < 0.05 vs. p53 group (right panel).

Article Snippet: Nonsmall cell lung cancer (NSCLC) cell lines A549 and PC-9 were all purchased from the American Type Culture Collection (ATCC).

Techniques: shRNA, Plasmid Preparation