Journal: Experimental and Therapeutic Medicine
Article Title: Overexpression of ELF1 combined with MMP9 is associated with prognosis and tumor microenvironment in gastric cancer
doi: 10.3892/etm.2024.12730
Figure Lengend Snippet: Association of ELF1 with CD19, CD3, CD4, CD8 and CD56 detected through immunohistochemical analysis. Magnification, x100; Scale bars, 250 µm). The red arrow indicates area of high protein expression, whilst the green arrow indicates areas of low protein expression. Upper, GC with ELF1 (+); lower, GC with ELF1 (-). ELF1, E74-like Factor 1.
Article Snippet: Subsequently, mouse antihuman ELF1 polyclonal antibody (dilution 1:400; cat. no. 22565-1-AP; ProteinTech Group, Inc.), MMP9 polyclonal antibody (dilution 1:300; cat. no. 10375-2-AP; ProteinTech Group, Inc.), mouse anti-human CD19 monoclonal (ready to use; cat. no. ZM-0038; ZSGB-BIO; OriGene Technologies, Inc.), mouse anti-human CD3 monoclonal (ready to use; cat. no. ZM-0417; ZSGB-BIO; OriGene Technologies, Inc.), mouse antihuman CD4 monoclonal (ready to use; cat. no. ZM-0418; ZSGB-BIO; OriGene Technologies, Inc.), rabbit anti-human CD8 monoclonal (ready to use; cat. no. ZM-0508 ZSGB-BIO; OriGene Technologies, Inc.) and mouse anti-human CD56 monoclonal (ready to use; cat. no. ZM-0057; ZSGB-BIO; OriGene Technologies, Inc.) were used for staining overnight at 4˚C.
Techniques: Immunohistochemical staining, Expressing