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normal human lung fibroblasts  (ATCC)


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    Structured Review

    ATCC normal human lung fibroblasts
    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
    Normal Human Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human lung fibroblasts/product/ATCC
    Average 86 stars, based on 1 article reviews
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    normal human lung fibroblasts - by Bioz Stars, 2024-12
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    Images

    1) Product Images from "YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer"

    Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer

    Journal: Bioscience Reports

    doi: 10.1042/BSR20241320

    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated fibroblasts stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
    Figure Legend Snippet: ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated fibroblasts stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).

    Techniques Used: Invasion Assay, Control, Contraction Assay



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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
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    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
    Human Lung Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human lung primary fibroblast cell line
    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated <t>fibroblasts</t> stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).
    Human Lung Primary Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated fibroblasts stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).

    Journal: Bioscience Reports

    Article Title: YAP signaling orchestrates the endothelin-1-guided invadopodia formation in high-grade serous ovarian cancer

    doi: 10.1042/BSR20241320

    Figure Lengend Snippet: ( A ) Invasion assay of PD HG-SOC cells silenced or not for RhoC or YAP for 72 h and stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of invading cells were photographed (scale bar: 100 µm, magnification 20×) ( left panels ) or counted ( right graph ). Bars are means ± SD (* P <0.002 vs. CTR; ** P <0.0002 vs. ET-1; n = 3). ( B ) IB analysis for MMP-2 and MMP-9 in total extracts of PD HG-SOC cells, silenced or not for RhoC or YAP for 72 h, stimulated or not with ET-1 and/or MAC for 24h. Tubulin was used as a loading control. Uncropped gels of Fig. B are shown in Supplementary Figure S7. ( C ) Collagen contraction assay of activated fibroblasts stimulated or not with ET-1 and/or treated with MAC for 24 h. Representative images of the collagen contraction were photographed ( left panels ). The right graph indicates the collagen gel area (cm 2 ). Bars are means ± SD (* P <0.0002 vs. untreated collagen; ** P <0.0002 vs. ET-1; n = 3).

    Article Snippet: Normal human lung fibroblasts (WI-38, CCL-75 ATCC) were cultured with Eagle’s minimum essential medium (EMEM) (30-2003 ATCC), supplemented with 10% FBS and 1% penicillin‒streptomycin.

    Techniques: Invasion Assay, Control, Contraction Assay