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human lung fibroblast cell line hlf  (ATCC)


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    ATCC human lung fibroblast cell line hlf
    Human Lung Fibroblast Cell Line Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 757 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 757 article reviews
    human lung fibroblast cell line hlf - by Bioz Stars, 2026-02
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    human lung fibroblast cell line hlf  (ATCC)
    97
    ATCC human lung fibroblast cell line hlf
    Human Lung Fibroblast Cell Line Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) <t>hTERT</t> RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.
    Cell Lines Htert Rpe1 Stearns Lab, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human lung fibroblast hlf cell line  (ATCC)
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    ATCC human lung fibroblast hlf cell line
    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) <t>hTERT</t> RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.
    Human Lung Fibroblast Hlf Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human lung fibroblast hlf mrc 5 cell line  (ATCC)
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    ATCC human lung fibroblast hlf mrc 5 cell line
    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) <t>hTERT</t> RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.
    Human Lung Fibroblast Hlf Mrc 5 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal human lung fibroblast cell line hlf-a  (Procell Inc)
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    Procell Inc normal human lung fibroblast cell line hlf-a
    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) <t>hTERT</t> RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.
    Normal Human Lung Fibroblast Cell Line Hlf A, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human lung fibroblast hlf 1 cell line  (ATCC)
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    ATCC human lung fibroblast hlf 1 cell line
    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) <t>hTERT</t> RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.
    Human Lung Fibroblast Hlf 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung fibroblast hlf 1 cell line/product/ATCC
    Average 97 stars, based on 1 article reviews
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    incubatedwith human lung fibroblasts hlf 1 cell line  (ATCC)
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    ATCC incubatedwith human lung fibroblasts hlf 1 cell line
    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) <t>hTERT</t> RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.
    Incubatedwith Human Lung Fibroblasts Hlf 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubatedwith human lung fibroblasts hlf 1 cell line/product/ATCC
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    human lung fibroblasts cell line hlf a  (ATCC)
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    ATCC human lung fibroblasts cell line hlf a
    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) <t>hTERT</t> RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.
    Human Lung Fibroblasts Cell Line Hlf A, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung fibroblasts cell line hlf a/product/ATCC
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    Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) hTERT RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.

    Journal: Molecular cell

    Article Title: Increasing cell size remodels the proteome and promotes senescence.

    doi: 10.1016/j.molcel.2022.07.017

    Figure Lengend Snippet: Figure 7. Cell volume-to-ploidy ratio drives size-dependent proteome changes (A) hTERT RPE-1 cells expressing fluorescent cell- cycle reporters (Cdt1-mKO2, Geminin-mAG) were treated with an Aurora kinase inhibitor barasertib (75 nM, 48 h) to partially inhibit cytokinesis. Cells were then sorted based on ploidy and G1 cell-cycle phase. The attainment of differently sized G1 cells was confirmed using a Coulter counter. The histo- gram shows a representative example of size distri- butions for sorted cells, and the numbers next to it represent mean cell size ± standard error for n = 3 biological replicates. (B) Ploidy-sorted and size-sorted G1 cells were iso- lated by FACS and their proteomes measured using TMT. Ploidy-sorted protein slope values were calcu- lated by plotting the relative protein concentration against the mean cell size in the 2N, 4N, and 8N bins to obtain a slope value (Table S9). (C) Distributions of ploidy-sorted and size-sorted protein slope values. Despite large increases in cell size from 2N to 8N, concentration changes were minimal. (D) Slightly negative correlation between ploidy- sorted and size-sorted protein slope values are consistent with a small increase in DNA-to-cell vol- ume ratio in polyploid G1 cells. x-axis bins are shown in dark blue. Error bars represent the 95% confi- dence interval, and r denotes the Pearson’s correla- tion coefficient. (E) DNA-to-cell-volume-dependent concentration changes for a representative set of proteins. For each protein panel, dotted lines represent unique peptide measurements.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Pierce BCA Protein Assay Kit Thermo Scientific Cat#23225 Sep-Pak 50mg C18 Cartridges Waters Cat##054955 High pH Revered-phased Peptide Fractionation Kit Thermo Scientific Cat#84868 Senescence b-Galactosidase Staining Kit Cell Signaling Technology Cat#9860 Direct-zol RNA Microprep Kit Zymo Research Cat#R2063 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat#C10424 CellTrace CFSE Cell Proliferation Kit Thermo Fisher Scientific Cat#C34554 Deposited data All Proteomics raw files and spectral searches This paper PRIDE: PXD033332 All RNAseq raw files This paper GEO: GSE208348 Experimental models: Cell lines hTERT RPE1 Stearns Lab (Stanford) RRID: CVCL_4388 Primary human fetal lung fibroblasts Cell Applications Cat#506-05f Software and algorithms FlowJo BD Biosciences, https://www.flowjo.com/solutions/flowjo RRID: SCR_008520 FIJI https://imagej.net/software/fiji/ RRID: SCR_002285 MaxQuant Mann Lab N/A Python - Jupyter Notebooks Anaconda N/A

    Techniques: Expressing, Protein Concentration, Concentration Assay