human lung cancer cell lines nci h1299  (ATCC)

Journal: OncoTargets and Therapy

Article Title: Prognostic Value of miR-10a-3p in Non-Small Cell Lung Cancer Patients

doi: 10.2147/OTT.S475644

Figure Lengend Snippet: miRNA expression pattern in lung cancer cell lines in contrast to normal lung cell line. ( A ) Venn diagram demonstrating counts of statistically significant differentially expressed miRNAs in H23, H1299, Calu-1, and A549 (>2 fold-change, FDR<0.05, at least 10 mapped reads per million transcripts in miRNA library) compared to normal lung cell line HBEC. The bold text represents 8 commonly deregulated miRNAs in the more aggressive Calu-1, H1299, and A549 cells. ( B ) Expression pattern of miRNAs differentially expressed in lung cancer H23, H1299, Calu-1, and A549.

Article Snippet: The human bronchial epithelial cell HBEC3 (ATCC No. CRL-4051) and human lung cancer cell lines NCI-H1299 (ATCC No. CRL-5803), A549 (ATCC No. CCL-185), Calu-1 (ATCC No. HTB-54) and NCI-H23 (ATCC No. CRL-5800) were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Biogenic silver nanoparticles incorporated hydrogel beads for anticancer and antibacterial activities

doi: 10.1038/s41598-024-79003-z

Figure Lengend Snippet: % Cell viability of CT and Ag-@ CT NPs against A549 cells. The data is expressed as mean ± SD (n = 3).

Article Snippet: Human lung A549 cancer cell line was obtained from the American Type Culture Collection (ATCC).

Techniques:

Journal: Scientific Reports

Article Title: Biogenic silver nanoparticles incorporated hydrogel beads for anticancer and antibacterial activities

doi: 10.1038/s41598-024-79003-z

Figure Lengend Snippet: Morphological appearance of A549 cells in phase contrast microscopy for (a) Control (b) CT and (c) Control (d) Ag@ CT NPs.

Article Snippet: Human lung A549 cancer cell line was obtained from the American Type Culture Collection (ATCC).

Techniques: Microscopy, Control

Journal: Scientific Reports

Article Title: Biogenic silver nanoparticles incorporated hydrogel beads for anticancer and antibacterial activities

doi: 10.1038/s41598-024-79003-z

Figure Lengend Snippet: Apoptotic development and morphological changes in A549 lung cancer cells upon treatment with (a) control (b) CT (c) control and (d) Ag@ CT NPs.

Article Snippet: Human lung A549 cancer cell line was obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: In Vivo

Article Title: The Effects of Nebivolol-Gefitinib-Loratadine Against Lung Cancer Cell Lines

doi: 10.21873/invivo.13746

Figure Lengend Snippet: The combinations of gefitinib (G), loratadine (L) and nebivolol (N) abolish the metabolic activity in A549 and NCI-H1975 cell lines. A) Drug-alone effect; B) L-IC 10 and N-IC 10 and their two- and three-drug combination effects on the A549 cell line; C) two-drug combinations effects at different ICs on both cell lines; D) three-drug combination effects. The combination of two or three drugs significantly inhibited metabolic activity in both cell lines compared to the effect of individual drugs. Cells were cultured in the presence of the drug combinations for 72 h and results are shown as the mean±S.D. of eight replicates for each group and from three different experiments. Statistically significant differences vs. the DMSO group (*), vs. L-IC 20 (▽) (or L-IC 10 in panel B), vs. L-IC 50 (∂) (or L-IC 20 in panel B), vs. N-IC 20 (φ) (or N-IC 10 in panel B), vs. N-IC 50 (●) (or N-IC 20 in panel B), vs. G-IC 20 (∍), vs. G-IC 50 (γ), vs. groups using G-IC 20 (♦) in the two-drug combination assay and vs. groups using G-IC 50 (φ) also in the two-drug combination assay. p<0.05 in all cases.

Article Snippet: The human lung cancer cell lines A549 and NCI-H1975 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured according to the suppliers instructions.

Techniques: Activity Assay, Cell Culture

Journal: In Vivo

Article Title: The Effects of Nebivolol-Gefitinib-Loratadine Against Lung Cancer Cell Lines

doi: 10.21873/invivo.13746

Figure Lengend Snippet: Apoptosis induction by gefitinib (G), loratadine (L), nebivolol (N), and their combinations in lung cancer cell lines. A) Nebivolol and drug combination increased apoptosis in comparison with the DMSO group in A549 cells. B) Drug combination increased apoptosis vs DMSO and drug-alone in NCI-H1975 cells. Cells were cultured in the presence of the combinations or individual drugs for 72 h. Results are shown as the mean±S.D. of three different experiments. C) Representative plots from the flow-cytometry data indicating the different quadrants separating the cell populations: viable cells (left panel), necrosis cells (center panel), apoptosis and late apoptosis cells (right panel). Statistically significant difference vs. the DMSO group (*), vs. L-IC 10 (▽) (or L-IC 20 in panel B), vs. N-IC 20 (∂), vs. G-IC 20 (φ) (or G-IC 50 in panel B). p<0.05 in all cases.

Article Snippet: The human lung cancer cell lines A549 and NCI-H1975 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured according to the suppliers instructions.

Techniques: Comparison, Cell Culture, Flow Cytometry

Journal: In Vivo

Article Title: The Effects of Nebivolol-Gefitinib-Loratadine Against Lung Cancer Cell Lines

doi: 10.21873/invivo.13746

Figure Lengend Snippet: Drug combinations of gefitinib (G), loratadine (L), and nebivolol (N) suppress cell survival in A549 and NCI-H1975 cells. The combinations tested were able to completely inhibit the formation of colonies. Percentage of colonies formed under each condition in A) A549 and B) NCI-H1975 cell lines. Cells were cultured in the presence of the individual drugs or their combinations or DMSO for 72 h. Results are shown as the mean±S.D. from three different assays. C) Representative examples of colony formation in Petri dishes, cells were stained with crystal violet (1%). Statistically significant differences vs. the DMSO group (*), vs. L-IC 10 (▽), vs. N-IC 10 (φ), vs. N-IC 20 (●), vs. G-IC 20 (∍), vs. G-IC 50 (γ). p<0.05 in all cases.

Article Snippet: The human lung cancer cell lines A549 and NCI-H1975 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured according to the suppliers instructions.

Techniques: Cell Culture, Staining