human inositol monophosphatase i himpasei cdna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human inositol monophosphatase i himpasei cdna
    Human Inositol Monophosphatase I Himpasei Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human inositol monophosphatase i himpasei cdna/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human inositol monophosphatase i himpasei cdna - by Bioz Stars, 2020-02
    78/100 stars

    Related Products / Commonly Used Together

    pcr
    expression vector pet15b
    cdna

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    Related Articles

    Amplification:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: .. Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. The hIMPase was over-expressed in Escherichia coli strain BL21(DE3) (Novagen) and purified using nickel-affinity chromatography (Ni-NTA, Qiagen, #1004493).

    Chromatography:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. The hIMPase was over-expressed in Escherichia coli strain BL21(DE3) (Novagen) and purified using nickel-affinity chromatography (Ni-NTA, Qiagen, #1004493).

    Purification:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. The hIMPase was over-expressed in Escherichia coli strain BL21(DE3) (Novagen) and purified using nickel-affinity chromatography (Ni-NTA, Qiagen, #1004493).

    Produced:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. Inositol monophosphatase activity in the presence of varying amounts of gal-1-p was measured by the amount of inositol produced using the method described by Dolhofer and Wieland [ ].

    Activity Assay:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. Inositol monophosphatase activity in the presence of varying amounts of gal-1-p was measured by the amount of inositol produced using the method described by Dolhofer and Wieland [ ].

    Expressing:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: .. Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. The hIMPase was over-expressed in Escherichia coli strain BL21(DE3) (Novagen) and purified using nickel-affinity chromatography (Ni-NTA, Qiagen, #1004493).

    Polymerase Chain Reaction:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: .. Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. The hIMPase was over-expressed in Escherichia coli strain BL21(DE3) (Novagen) and purified using nickel-affinity chromatography (Ni-NTA, Qiagen, #1004493).

    Plasmid Preparation:

    Article Title: INVOLVEMENT OF ENDOPLASMIC RETICULUM STRESS IN A NOVEL CLASSIC GALACTOSEMIA MODEL
    Article Snippet: .. Human inositol monophosphatase I (hIMPaseI) cDNA clone was purchased from Open Biosystems. cDNA was amplified by PCR and subcloned into the bacterial expression vector pET15b (Novagen). .. The hIMPase was over-expressed in Escherichia coli strain BL21(DE3) (Novagen) and purified using nickel-affinity chromatography (Ni-NTA, Qiagen, #1004493).