rhil 7  (R&D Systems)

 
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    Name:
    Recombinant Human IL 7 Protein CF
    Description:
    The Recombinant Human IL 7 Protein from R D Systems is derived from E coli The Recombinant Human IL 7 Protein has been validated for the following applications Bioactivity
    Catalog Number:
    207-IL-005/CF
    Price:
    229
    Category:
    Proteins and Enzymes
    Source:
    E. coli-derived Recombinant Human IL-7 Protein
    Applications:
    Bioactivity
    Purity:
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Conjugate:
    Unconjugated
    Size:
    5 ug
    Buy from Supplier


    Structured Review

    R&D Systems rhil 7
    <t>rhIL-7</t> prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    The Recombinant Human IL 7 Protein from R D Systems is derived from E coli The Recombinant Human IL 7 Protein has been validated for the following applications Bioactivity
    https://www.bioz.com/result/rhil 7/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rhil 7 - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    2) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    3) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    4) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    5) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    6) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    7) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    8) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    9) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    10) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    11) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    12) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    13) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    14) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    15) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    16) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    17) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    18) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    19) Product Images from "IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis"

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0903151

    rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in
    Figure Legend Snippet: rhIL-7 prevents loss of CD4 and CD8 T cells in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 or diluent 90 min after surgery. Spleens were harvested for determination of absolute cell counts ~24 h later. Sepsis induced a loss in

    Techniques Used: Mouse Assay

    rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1
    Figure Legend Snippet: rhIL-7 reverses the sepsis-induced defect in IFN-γ production. A , Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min after surgery. Twenty-four hours later, splenocytes were harvested, and 1

    Techniques Used: Mouse Assay

    rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes
    Figure Legend Snippet: rhIL-7 preserves naive, central memory, and effector memory CD4 and CD8 T cells in the spleen and regional lymph nodes

    Techniques Used:

    rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained
    Figure Legend Snippet: rhIL-7 increases LFA-1, VLA-4, CD4, and CD8 expression on T cells. Mice underwent sham or CLP surgery, and selected groups were treated with 5 μg of rhIL-7 90 min and 24 and 48 h after surgery. Splenocytes were harvested 72 h later and stained

    Techniques Used: Expressing, Mouse Assay, Staining

    rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation
    Figure Legend Snippet: rhIL-7 increases cell proliferation. A , Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery and again 24 and 48 h later. At 72 h after surgery, spleens were harvested, and lymphocytes were stained for the proliferation

    Techniques Used: Mouse Assay, Staining

    rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked
    Figure Legend Snippet: rhIL-7 prevents sepsis-induced apoptosis of T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Spleens were harvested 24 h later, and apoptosis was determined by the TUNEL method. rhIL-7 prevented the marked

    Techniques Used: Mouse Assay, TUNEL Assay

    rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets
    Figure Legend Snippet: rhIL-7 increases CD4 and CD8 T cell subsets in lymph nodes. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery. Mesenteric lymph nodes were harvested 24 h later, and central memory, naive, and effector memory T cell subsets

    Techniques Used: Mouse Assay

    rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens
    Figure Legend Snippet: rhIL-7 increases antiapoptotic Bcl-2 and decreases proapoptotic PUMA protein and mRNA. A , Flow cytometry of intracellular staining for Bcl-2 in CD4 T cells. Sham or septic mice were treated with 5 μg of rhIL-7 at 90 min after surgery, spleens

    Techniques Used: Flow Cytometry, Cytometry, Staining, Mouse Assay

    IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment
    Figure Legend Snippet: IL-7R expression is increased in sepsis. Sham or septic mice were treated with 5 μg of rhIL-7 for three consecutive days and killed on the third postoperative day. Sepsis induced a marked increase in IL-7R expression in CD4 and CD8 T cells. Treatment

    Techniques Used: Expressing, Mouse Assay

    rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly
    Figure Legend Snippet: rhIL-7 improves survival in sepsis. A , CD-1 mice were injected with 5 μg of rhIL-7 90 min after CLP and again at 24 and 48 h. Septic control mice received the saline diluent. Survival was recorded for 7 d. Mice receiving rhIL-7 had a markedly

    Techniques Used: Mouse Assay, Injection

    Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,
    Figure Legend Snippet: Sepsis induces a loss in the DTH response that is prevented by rhIL-7. Mice underwent sham or CLP surgery, and selected groups were treated with 2.5 μg of rhIL-7 90 min postsurgery and every 48 h for two additional doses. Four days postsurgery,

    Techniques Used: Mouse Assay

    Related Articles

    other:

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: Sepsis induced major decreases in absolute cell counts in all five cell phenotypes examined, including CD4 T cells (49.3% ± 6.5% loss), CD8 T cells (45.2% ± 6.7% loss), B cells (40.2% ± 7.3% loss), NK cells (54.9% ± 7.9% loss), and dendritic cells (48.5% ± 5.7% loss) ( ). rhIL-7 effectively prevented the sepsis-induced loss in CD4 and CD8 T cells, such that absolute cell counts were not statistically different in the sham plus rhIL-7 group versus the CLP plus rhIL-7 group. rhIL-7 had no effect on the sepsis-induced loss of B cells, NK cells, or dendritic cells ( ).

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: Five micrograms of rhIL-7 was administered 90 min after sham or CLP surgery.

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: To determine whether rhIL-7 prevented the loss of CD4 and CD8 T cells throughout the duration of sepsis, absolute cell counts for CD4 and CD8 T cells were quantified at 4 d postsham or CLP surgery.

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: A series of mechanistic studies was performed to determine potential means by which rhIL-7 improves survival.

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: The protective effect of IL-7 to prevent depletion of tissue immune effector cells was also seen in the thymus. rhIL-7 decreased the sepsis-induced loss in double-positive, double-negative, and single-positive CD4/CD8 thymocytes (Supplemental Fig. 2).

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: This latter finding suggests that rhIL-7 did not prime cells for excessive production, but rather reversed the sepsis-induced defect.

    Mouse Assay:

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: This latter finding suggests that rhIL-7 did not prime cells for excessive production, but rather reversed the sepsis-induced defect. .. One possible explanation for the increase in IFN-γ production in the splenocytes from septic mice treated with rhIL-7 is that splenocytes from rhIL-7–treated mice had improved survival during the overnight incubation and, therefore, produced more IFN-γ. .. It is also possible that rhIL-7 improved the ability of the individual splenocytes to produce more IFN-γ.

    Incubation:

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: This latter finding suggests that rhIL-7 did not prime cells for excessive production, but rather reversed the sepsis-induced defect. .. One possible explanation for the increase in IFN-γ production in the splenocytes from septic mice treated with rhIL-7 is that splenocytes from rhIL-7–treated mice had improved survival during the overnight incubation and, therefore, produced more IFN-γ. .. It is also possible that rhIL-7 improved the ability of the individual splenocytes to produce more IFN-γ.

    Produced:

    Article Title: IL-7 Promotes T Cell Viability, Trafficking, and Functionality and Improves Survival in Sepsis
    Article Snippet: This latter finding suggests that rhIL-7 did not prime cells for excessive production, but rather reversed the sepsis-induced defect. .. One possible explanation for the increase in IFN-γ production in the splenocytes from septic mice treated with rhIL-7 is that splenocytes from rhIL-7–treated mice had improved survival during the overnight incubation and, therefore, produced more IFN-γ. .. It is also possible that rhIL-7 improved the ability of the individual splenocytes to produce more IFN-γ.

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    R&D Systems interleukin 7
    Expression of lytic molecules during the in vitro expansion of primary CD8 + T cells. (A) Expression of GrA, GrB and Pfp in human PBMC and purified CD8 + T cells at days 0, 7 and 17 after stimulation with anti-CD3/CD28 mAb-coated beads in the presence of IL-2, <t>Il-7</t> and IL-15. Cells were stained with anti-CD8, anti-GrA, anti-GrB and anti-Pfp mAbs and analyzed by flow cytometry. The expression levels of GrA, GrB and Pfp are plotted against the CD8 marker. The values in each plot show the frequency of each subset in the cell population. The data are from one representative donor out of 3 analyzed in a similar way. (B) Frequency of the cells expressing the three lytic molecules GrA, GrB and Pfp. Percentage of co-expression was measured by first gating the CD8 and GrA positive cells and then on the GrB and Pfp positive cells. The mean percentage and SD of the resulting population at days 0, 7 and 17 are shown (n = 3). (C) The mean fluorescence intensity (MFI) of GrA, GrB and Pfp for CD8 + cells at days 0, 7 and 17 are shown (n = 3).
    Interleukin 7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems antibodies against il 7
    Interferon-response factor-1 (IRF1) transcription exaggerates interferon (IFN)-induced <t>interleukin-7</t> <t>(IL-7)</t> expression. (A) Quantification of IRF-1 mRNA levels relative to housekeeping GAPDH mRNA in Huh-7.5 cells, which were treated for the indicated time points with IFN-α (500 i.E./ml), IFN-γ (5 ng/ml), or IFN-λ2 (50 ng/ml). IRF-1 mRNA levels relative to housekeeping GAPDH mRNA are expressed relative to untreated cells. Standard deviations of three experiments (n=3) performed with three replicates each are shown. * P
    Antibodies Against Il 7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against il 7/product/R&D Systems
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    R&D Systems cytokine cocktail mix
    Multiparametric functional assessment of EBV- and CMV-specific CD8 + T cells in SLE patients. ( A ) Representative cytofluorometric detection (left) and functional analysis (right) of CD8 + T cells specific for one of the lytic EBV antigens tested (BZLF1) in a healthy control (upper panel) and in an inactive SLE patient (lover panel) post peptide antigen stimulation of PBMC. Lytic EBV and CMV antigen-specific cells were detected with peptide/MHC tetramer and anti-CD8 antibody (red box) and simultaneously analyzed for intra-cellular IFN-γ, TNF-α, IL-2 and MIP-1β content. <t>Cytokine/chemokine</t> gates were positioned according to control stains of non-stimulated virus-specific T cells. (B) Magnitude and (C) functionality of EBV- (upper panel) and CMV-specific (lower panel) responses in healthy controls (H, n = 26 and 15, respectively), inactive (i, n = 19 and 10) and active (a, n = 27 and 11) SLE patients. (D) EBV-specific T cells (upper panel) are strikingly less polyfunctional in inactive (iSLE) and active (aSLE) SLE patients compared to controls (healthy), while polyfunctionality of CMV-specific responses (lower panel) is preserved. Pie representations of virus-specific CD8 + T cells represent the fraction of individual cells secreting none (0) or any (1, 2, 3 or 4) of the four cytokines IFN-γ, TNF-α, IL-2 and MIP-1β (color coded as indicated). E.g. the red pie slice indicates the proportion of cells producing four cytokines (IFN-γ, TNF-α, IL-2 and MIP-1β). P -values monitoring differences between healthy donors and SLE patients are calculated using a non-parametric Mann-Whitney test and pie comparison statistics of the Spice software.
    Cytokine Cocktail Mix, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Human IL 7 Biotinylated Antibody from R D Systems is a goat polyclonal antibody to IL 7 This antibody reacts with human The Human IL 7 Biotinylated Antibody has
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    Expression of lytic molecules during the in vitro expansion of primary CD8 + T cells. (A) Expression of GrA, GrB and Pfp in human PBMC and purified CD8 + T cells at days 0, 7 and 17 after stimulation with anti-CD3/CD28 mAb-coated beads in the presence of IL-2, Il-7 and IL-15. Cells were stained with anti-CD8, anti-GrA, anti-GrB and anti-Pfp mAbs and analyzed by flow cytometry. The expression levels of GrA, GrB and Pfp are plotted against the CD8 marker. The values in each plot show the frequency of each subset in the cell population. The data are from one representative donor out of 3 analyzed in a similar way. (B) Frequency of the cells expressing the three lytic molecules GrA, GrB and Pfp. Percentage of co-expression was measured by first gating the CD8 and GrA positive cells and then on the GrB and Pfp positive cells. The mean percentage and SD of the resulting population at days 0, 7 and 17 are shown (n = 3). (C) The mean fluorescence intensity (MFI) of GrA, GrB and Pfp for CD8 + cells at days 0, 7 and 17 are shown (n = 3).

    Journal: PLoS ONE

    Article Title: Stepwise Maturation of Lytic Granules during Differentiation and Activation of Human CD8+ T Lymphocytes

    doi: 10.1371/journal.pone.0027057

    Figure Lengend Snippet: Expression of lytic molecules during the in vitro expansion of primary CD8 + T cells. (A) Expression of GrA, GrB and Pfp in human PBMC and purified CD8 + T cells at days 0, 7 and 17 after stimulation with anti-CD3/CD28 mAb-coated beads in the presence of IL-2, Il-7 and IL-15. Cells were stained with anti-CD8, anti-GrA, anti-GrB and anti-Pfp mAbs and analyzed by flow cytometry. The expression levels of GrA, GrB and Pfp are plotted against the CD8 marker. The values in each plot show the frequency of each subset in the cell population. The data are from one representative donor out of 3 analyzed in a similar way. (B) Frequency of the cells expressing the three lytic molecules GrA, GrB and Pfp. Percentage of co-expression was measured by first gating the CD8 and GrA positive cells and then on the GrB and Pfp positive cells. The mean percentage and SD of the resulting population at days 0, 7 and 17 are shown (n = 3). (C) The mean fluorescence intensity (MFI) of GrA, GrB and Pfp for CD8 + cells at days 0, 7 and 17 are shown (n = 3).

    Article Snippet: CD8+ T cells were stimulated and expanded with anti-CD3/anti-CD28 T-Cell Expander Dynabeads™ (Invitrogen) in RPMI 1640 medium supplemented with 10% FCS, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 10 mM Hepes, 50 U/ml Penicillin, 50 µg/ml Streptomycin (all from Invitrogen), 50 µM b-Mercaptoethanol, 150 U/ml Interleukin-2 (Chiron/Proleukin), 10 ng/ml Interleukin-7 (R & D System) and 5 ng/ml Interleukin-15 (R & D System).

    Techniques: Expressing, In Vitro, Purification, Staining, Flow Cytometry, Cytometry, Marker, Fluorescence

    Interferon-response factor-1 (IRF1) transcription exaggerates interferon (IFN)-induced interleukin-7 (IL-7) expression. (A) Quantification of IRF-1 mRNA levels relative to housekeeping GAPDH mRNA in Huh-7.5 cells, which were treated for the indicated time points with IFN-α (500 i.E./ml), IFN-γ (5 ng/ml), or IFN-λ2 (50 ng/ml). IRF-1 mRNA levels relative to housekeeping GAPDH mRNA are expressed relative to untreated cells. Standard deviations of three experiments (n=3) performed with three replicates each are shown. * P

    Journal: Frontiers in Immunology

    Article Title: Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity

    doi: 10.3389/fimmu.2020.581352

    Figure Lengend Snippet: Interferon-response factor-1 (IRF1) transcription exaggerates interferon (IFN)-induced interleukin-7 (IL-7) expression. (A) Quantification of IRF-1 mRNA levels relative to housekeeping GAPDH mRNA in Huh-7.5 cells, which were treated for the indicated time points with IFN-α (500 i.E./ml), IFN-γ (5 ng/ml), or IFN-λ2 (50 ng/ml). IRF-1 mRNA levels relative to housekeeping GAPDH mRNA are expressed relative to untreated cells. Standard deviations of three experiments (n=3) performed with three replicates each are shown. * P

    Article Snippet: Neutralizing antibodies against IL-7 (MAB207) and IFNAR1 (AF245) were from R & D Systems.

    Techniques: Expressing

    The IFN-IRF-1-IL-7 axis is present in patients with liver cirrhosis. (A) Presence of the proteins interferon-response factor-1 (IRF1), Glycogen synthase kinase-3 (GSK3), CD68 as a macrophage marker, and CK18 as part of the hepatocellular cytoskeleton was analyzed by immunofluorescence in paraffin-embedded tissue of a representative patient with liver cirrhosis. One out of two experiments (n=2) is shown as demonstration. (B) Interferon-stimulated gene (ISG)-15 relative to GAPDH mRNA concentration was quantified by quantitative PCR in (PBMCs of 11 randomly selected patients with liver cirrhosis from (C) and correlated with serum concentrations of interleukin-7 (IL-7). P value for Pearson correlation. (C) Flow cytometric analyses of the percentage of CD127 positive myeloid cells relative to all myeloid cells from patients with liver cirrhosis and available PBMCs or healthy controls. (D) Serum concentrations of IL-7 and interferon-(IFN)-γ in healthy controls and in patients with different liver cirrhosis stages or with acute-on-chronic liver failure (ACLF) were determined by ELISA. P for comparison between healthy controls and all patients with liver cirrhosis. (E) Monocytes from peripheral blood and ascites-derived macrophages of patients with ascites (n=4), compensated liver cirrhosis (n=4) and decompensated liver cirrhosis (n=4, all measured in duplicate) were stimulated ex vivo with 10 ng/ml IL-7 and/or 10 ng/ml LPS, as indicated, for 6 h. Then, quantitative analysis of mRNA of IL-1β, IL-6, and tumor necrosis factor α (TNFα) levels relative to housekeeping GAPDH mRNA was performed. * P

    Journal: Frontiers in Immunology

    Article Title: Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity

    doi: 10.3389/fimmu.2020.581352

    Figure Lengend Snippet: The IFN-IRF-1-IL-7 axis is present in patients with liver cirrhosis. (A) Presence of the proteins interferon-response factor-1 (IRF1), Glycogen synthase kinase-3 (GSK3), CD68 as a macrophage marker, and CK18 as part of the hepatocellular cytoskeleton was analyzed by immunofluorescence in paraffin-embedded tissue of a representative patient with liver cirrhosis. One out of two experiments (n=2) is shown as demonstration. (B) Interferon-stimulated gene (ISG)-15 relative to GAPDH mRNA concentration was quantified by quantitative PCR in (PBMCs of 11 randomly selected patients with liver cirrhosis from (C) and correlated with serum concentrations of interleukin-7 (IL-7). P value for Pearson correlation. (C) Flow cytometric analyses of the percentage of CD127 positive myeloid cells relative to all myeloid cells from patients with liver cirrhosis and available PBMCs or healthy controls. (D) Serum concentrations of IL-7 and interferon-(IFN)-γ in healthy controls and in patients with different liver cirrhosis stages or with acute-on-chronic liver failure (ACLF) were determined by ELISA. P for comparison between healthy controls and all patients with liver cirrhosis. (E) Monocytes from peripheral blood and ascites-derived macrophages of patients with ascites (n=4), compensated liver cirrhosis (n=4) and decompensated liver cirrhosis (n=4, all measured in duplicate) were stimulated ex vivo with 10 ng/ml IL-7 and/or 10 ng/ml LPS, as indicated, for 6 h. Then, quantitative analysis of mRNA of IL-1β, IL-6, and tumor necrosis factor α (TNFα) levels relative to housekeeping GAPDH mRNA was performed. * P

    Article Snippet: Neutralizing antibodies against IL-7 (MAB207) and IFNAR1 (AF245) were from R & D Systems.

    Techniques: Marker, Immunofluorescence, Concentration Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Derivative Assay, Ex Vivo

    Inducible interleukin-7 (IL-7)-expression in the hepatic system relies on interferon-response factor-1 (IRF1) protein translation. (A) Huh7.5 cells underwent gene silencing by treatment with IRF-1 siRNA or unspecific control small interfering RNA (siRNA) for 24 h. After removing the siRNA, cells recovered for 24 h before being treated with 5 ng/ml interferon- (IFN)-γ for 6 h. Levels of IL-7 mRNA relative to housekeeping GAPDH mRNA are expressed relative to untreated cells. Standard deviations of two independent experiments (n=2) performed with three replicates each are shown. * P

    Journal: Frontiers in Immunology

    Article Title: Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity

    doi: 10.3389/fimmu.2020.581352

    Figure Lengend Snippet: Inducible interleukin-7 (IL-7)-expression in the hepatic system relies on interferon-response factor-1 (IRF1) protein translation. (A) Huh7.5 cells underwent gene silencing by treatment with IRF-1 siRNA or unspecific control small interfering RNA (siRNA) for 24 h. After removing the siRNA, cells recovered for 24 h before being treated with 5 ng/ml interferon- (IFN)-γ for 6 h. Levels of IL-7 mRNA relative to housekeeping GAPDH mRNA are expressed relative to untreated cells. Standard deviations of two independent experiments (n=2) performed with three replicates each are shown. * P

    Article Snippet: Neutralizing antibodies against IL-7 (MAB207) and IFNAR1 (AF245) were from R & D Systems.

    Techniques: Expressing, Small Interfering RNA

    Phosphorylation of Glycogen synthase kinase-3 (GSK3) in dendritic cells (DCs) and macrophages in response to interleukin-7 (IL-7). (A) Naive CD4 T cells were treated with ascending concentrations of human IL-7 for 15 min. Presence of phosphorylated and total Stat5 was analyzed by Western Blot. (B) Human monocytes were differentiated into monocyte-derived DCs (left panel) or monocyte-derived macrophages (MDMs) (right panel). Cells were pre-incubated with 10 ng/ml lipopolysaccharide (LPS) for 48 h before treatment with the displayed concentrations of IL-7 for 15 min. Presence and phosphorylation status of proteins (i.e. Serine 21/9 phosphorylation on GSK3α and GSK3β respectively and Tyrosine 694 phosphorylation on Stat5) was assessed using Western Blot with β-Actin as control. (C) monocyte-derived macrophages (MDMs) and DCs were stimulated with or without 10 ng/ml LPS for 16 h before incubation with the different concentrations of IL-7 (10 pg/ml or 10 ng/ml) for 15 min. Presence and phosphorylation status of proteins (i.e. Serine 21/9 phosphorylation on GSK3α and GSK3β respectively and Tyrosine 694 phosphorylation on Stat5) was assessed using Western Blot with β-Actin as control. In (A–C) , one out of three experiments (n=3) is shown as demonstration.

    Journal: Frontiers in Immunology

    Article Title: Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity

    doi: 10.3389/fimmu.2020.581352

    Figure Lengend Snippet: Phosphorylation of Glycogen synthase kinase-3 (GSK3) in dendritic cells (DCs) and macrophages in response to interleukin-7 (IL-7). (A) Naive CD4 T cells were treated with ascending concentrations of human IL-7 for 15 min. Presence of phosphorylated and total Stat5 was analyzed by Western Blot. (B) Human monocytes were differentiated into monocyte-derived DCs (left panel) or monocyte-derived macrophages (MDMs) (right panel). Cells were pre-incubated with 10 ng/ml lipopolysaccharide (LPS) for 48 h before treatment with the displayed concentrations of IL-7 for 15 min. Presence and phosphorylation status of proteins (i.e. Serine 21/9 phosphorylation on GSK3α and GSK3β respectively and Tyrosine 694 phosphorylation on Stat5) was assessed using Western Blot with β-Actin as control. (C) monocyte-derived macrophages (MDMs) and DCs were stimulated with or without 10 ng/ml LPS for 16 h before incubation with the different concentrations of IL-7 (10 pg/ml or 10 ng/ml) for 15 min. Presence and phosphorylation status of proteins (i.e. Serine 21/9 phosphorylation on GSK3α and GSK3β respectively and Tyrosine 694 phosphorylation on Stat5) was assessed using Western Blot with β-Actin as control. In (A–C) , one out of three experiments (n=3) is shown as demonstration.

    Article Snippet: Neutralizing antibodies against IL-7 (MAB207) and IFNAR1 (AF245) were from R & D Systems.

    Techniques: Western Blot, Derivative Assay, Incubation

    Interleukin-7 (IL-7) decreases intracellular ATP content of monocyte-derived macrophages but augments pro-inflammatory cytokine production in response to lipopolysaccharide (LPS). (A) Monocyte-derived macrophages (MDMs) underwent pre-incubation with or without 10 ng/ml LPS for 16 h before being stimulated with IL-7 or the Glycogen synthase kinase-3 (GSK3) Inhibitor CHIR99021 (5 µM) for the displayed time points. Then the intracellular ATP content relative to unstimulated controls was analyzed. Standard deviations of three experiments (n=3) performed with three replicates each are shown. * P

    Journal: Frontiers in Immunology

    Article Title: Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity

    doi: 10.3389/fimmu.2020.581352

    Figure Lengend Snippet: Interleukin-7 (IL-7) decreases intracellular ATP content of monocyte-derived macrophages but augments pro-inflammatory cytokine production in response to lipopolysaccharide (LPS). (A) Monocyte-derived macrophages (MDMs) underwent pre-incubation with or without 10 ng/ml LPS for 16 h before being stimulated with IL-7 or the Glycogen synthase kinase-3 (GSK3) Inhibitor CHIR99021 (5 µM) for the displayed time points. Then the intracellular ATP content relative to unstimulated controls was analyzed. Standard deviations of three experiments (n=3) performed with three replicates each are shown. * P

    Article Snippet: Neutralizing antibodies against IL-7 (MAB207) and IFNAR1 (AF245) were from R & D Systems.

    Techniques: Derivative Assay, Incubation

    Interferon-(IFN)-α and IFN-γ, but not IFN-λ, induce hepatic interleukin-7 (IL-7) expression and secretion. (A) Quantification of IL-7 mRNA levels in relation to housekeeping GAPDH mRNA in Huh-7.5 cells, which were stimulated for the indicated hours with IFN-α (left), IFN-γ (middle), or IFN-λ2 (right) at the indicated dosages. IL-7 expression is shown relative to untreated cells. (B) IFN-λ signaling is present in Huh-7.5 cells. Quantification of interferon-stimulated gene (ISG) mRNA levels relative to housekeeping GAPDH mRNA in Huh-7.5 cells, which were stimulated with IFN-α, IFN-γ, or IFN-λ2 at the indicated dosages for 6 h. ISG expression is shown relative to untreated cells. (C) Analysis of protein levels of IL- via ELISA in cell culture supernatant of Huh7.5 cells and (D) primary human hepatic sinusoidal endothelial cell (HLSEC), which were stimulated with IFN-α (500 i.E./ml), IFN-γ (5 ng/ml), or IFN-λ2 (50 ng/ml) for 6 or 24 h. In (A–D) , standard deviations of three independent experiments (n=3) performed with three replicates each are shown. * P

    Journal: Frontiers in Immunology

    Article Title: Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity

    doi: 10.3389/fimmu.2020.581352

    Figure Lengend Snippet: Interferon-(IFN)-α and IFN-γ, but not IFN-λ, induce hepatic interleukin-7 (IL-7) expression and secretion. (A) Quantification of IL-7 mRNA levels in relation to housekeeping GAPDH mRNA in Huh-7.5 cells, which were stimulated for the indicated hours with IFN-α (left), IFN-γ (middle), or IFN-λ2 (right) at the indicated dosages. IL-7 expression is shown relative to untreated cells. (B) IFN-λ signaling is present in Huh-7.5 cells. Quantification of interferon-stimulated gene (ISG) mRNA levels relative to housekeeping GAPDH mRNA in Huh-7.5 cells, which were stimulated with IFN-α, IFN-γ, or IFN-λ2 at the indicated dosages for 6 h. ISG expression is shown relative to untreated cells. (C) Analysis of protein levels of IL- via ELISA in cell culture supernatant of Huh7.5 cells and (D) primary human hepatic sinusoidal endothelial cell (HLSEC), which were stimulated with IFN-α (500 i.E./ml), IFN-γ (5 ng/ml), or IFN-λ2 (50 ng/ml) for 6 or 24 h. In (A–D) , standard deviations of three independent experiments (n=3) performed with three replicates each are shown. * P

    Article Snippet: Neutralizing antibodies against IL-7 (MAB207) and IFNAR1 (AF245) were from R & D Systems.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture

    Interleukin-7 (IL-7) decreases monocyte-derived macrophage viability. (A, B) Human monocyte-derived macrophages (MDMs) were pre-stimulated with (B) or without (A) 10 ng/ml lipopolysaccharide (LPS) for 16 h. Subsequently, cells were stimulated with the displayed concentrations of IL-7 or the Glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021. Cellular viability was estimated using the WST-1 reagent at an absorbance of 450 nm relative to unstimulated cells. Standard deviations of three experiments (n=3) performed with three replicates each are shown. * P

    Journal: Frontiers in Immunology

    Article Title: Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity

    doi: 10.3389/fimmu.2020.581352

    Figure Lengend Snippet: Interleukin-7 (IL-7) decreases monocyte-derived macrophage viability. (A, B) Human monocyte-derived macrophages (MDMs) were pre-stimulated with (B) or without (A) 10 ng/ml lipopolysaccharide (LPS) for 16 h. Subsequently, cells were stimulated with the displayed concentrations of IL-7 or the Glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021. Cellular viability was estimated using the WST-1 reagent at an absorbance of 450 nm relative to unstimulated cells. Standard deviations of three experiments (n=3) performed with three replicates each are shown. * P

    Article Snippet: Neutralizing antibodies against IL-7 (MAB207) and IFNAR1 (AF245) were from R & D Systems.

    Techniques: Derivative Assay

    Multiparametric functional assessment of EBV- and CMV-specific CD8 + T cells in SLE patients. ( A ) Representative cytofluorometric detection (left) and functional analysis (right) of CD8 + T cells specific for one of the lytic EBV antigens tested (BZLF1) in a healthy control (upper panel) and in an inactive SLE patient (lover panel) post peptide antigen stimulation of PBMC. Lytic EBV and CMV antigen-specific cells were detected with peptide/MHC tetramer and anti-CD8 antibody (red box) and simultaneously analyzed for intra-cellular IFN-γ, TNF-α, IL-2 and MIP-1β content. Cytokine/chemokine gates were positioned according to control stains of non-stimulated virus-specific T cells. (B) Magnitude and (C) functionality of EBV- (upper panel) and CMV-specific (lower panel) responses in healthy controls (H, n = 26 and 15, respectively), inactive (i, n = 19 and 10) and active (a, n = 27 and 11) SLE patients. (D) EBV-specific T cells (upper panel) are strikingly less polyfunctional in inactive (iSLE) and active (aSLE) SLE patients compared to controls (healthy), while polyfunctionality of CMV-specific responses (lower panel) is preserved. Pie representations of virus-specific CD8 + T cells represent the fraction of individual cells secreting none (0) or any (1, 2, 3 or 4) of the four cytokines IFN-γ, TNF-α, IL-2 and MIP-1β (color coded as indicated). E.g. the red pie slice indicates the proportion of cells producing four cytokines (IFN-γ, TNF-α, IL-2 and MIP-1β). P -values monitoring differences between healthy donors and SLE patients are calculated using a non-parametric Mann-Whitney test and pie comparison statistics of the Spice software.

    Journal: PLoS Pathogens

    Article Title: Exhausted Cytotoxic Control of Epstein-Barr Virus in Human Lupus

    doi: 10.1371/journal.ppat.1002328

    Figure Lengend Snippet: Multiparametric functional assessment of EBV- and CMV-specific CD8 + T cells in SLE patients. ( A ) Representative cytofluorometric detection (left) and functional analysis (right) of CD8 + T cells specific for one of the lytic EBV antigens tested (BZLF1) in a healthy control (upper panel) and in an inactive SLE patient (lover panel) post peptide antigen stimulation of PBMC. Lytic EBV and CMV antigen-specific cells were detected with peptide/MHC tetramer and anti-CD8 antibody (red box) and simultaneously analyzed for intra-cellular IFN-γ, TNF-α, IL-2 and MIP-1β content. Cytokine/chemokine gates were positioned according to control stains of non-stimulated virus-specific T cells. (B) Magnitude and (C) functionality of EBV- (upper panel) and CMV-specific (lower panel) responses in healthy controls (H, n = 26 and 15, respectively), inactive (i, n = 19 and 10) and active (a, n = 27 and 11) SLE patients. (D) EBV-specific T cells (upper panel) are strikingly less polyfunctional in inactive (iSLE) and active (aSLE) SLE patients compared to controls (healthy), while polyfunctionality of CMV-specific responses (lower panel) is preserved. Pie representations of virus-specific CD8 + T cells represent the fraction of individual cells secreting none (0) or any (1, 2, 3 or 4) of the four cytokines IFN-γ, TNF-α, IL-2 and MIP-1β (color coded as indicated). E.g. the red pie slice indicates the proportion of cells producing four cytokines (IFN-γ, TNF-α, IL-2 and MIP-1β). P -values monitoring differences between healthy donors and SLE patients are calculated using a non-parametric Mann-Whitney test and pie comparison statistics of the Spice software.

    Article Snippet: Blockade of PD-1 signal pathway PBMCs were cultured for 10 days at 37°C 5% C02, in RPMI supplemented with 5% human serum and a cytokine cocktail mix (20 ng/ml of IL-7 and 20 ng/ml IL-2 (R & D Systems, Minneapolis, MN)).

    Techniques: Functional Assay, MANN-WHITNEY, Software

    IL7R* CC MS patients have increased plasma IL-7 levels. ( A ) IL-7 levels were measured in plasma and CSF from patients with MS according to genotype. IL7R* CC patients had significantly higher mean plasma IL-7 levels than IL7R* TT patients, whereas no difference was observed between IL7R*CC and IL7R*CT heterozygotes. No correlation between CSF IL-7 levels and IL7R genotype was observed. The dotted line denotes limit of detection. ( B ) Plasma IL-7 levels were measured in plasma from a second independent cohort of patients with MS, as well as a separate cohort of healthy controls. IL7R* CC MS patients have significantly higher mean IL-7 levels than IL7R* TT MS patients, whereas no difference was observed according to genotype in healthy controls. Each shape represents a plasma or CSF sample analyzed from one patient or healthy control.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Soluble IL7R? potentiates IL-7 bioactivity and promotes autoimmunity

    doi: 10.1073/pnas.1222303110

    Figure Lengend Snippet: IL7R* CC MS patients have increased plasma IL-7 levels. ( A ) IL-7 levels were measured in plasma and CSF from patients with MS according to genotype. IL7R* CC patients had significantly higher mean plasma IL-7 levels than IL7R* TT patients, whereas no difference was observed between IL7R*CC and IL7R*CT heterozygotes. No correlation between CSF IL-7 levels and IL7R genotype was observed. The dotted line denotes limit of detection. ( B ) Plasma IL-7 levels were measured in plasma from a second independent cohort of patients with MS, as well as a separate cohort of healthy controls. IL7R* CC MS patients have significantly higher mean IL-7 levels than IL7R* TT MS patients, whereas no difference was observed according to genotype in healthy controls. Each shape represents a plasma or CSF sample analyzed from one patient or healthy control.

    Article Snippet: Human IL-7 levels in human plasma, mouse plasma, and culture supernatants were measured by high-sensitivity IL-7 ELISA kit according to the manufacturer’s instructions (Quantikine; R & D Systems).

    Techniques: Mass Spectrometry

    sIL7RΑ potentiates IL-7–mediated survival and diminishes consumption of IL-7. ( A ) Survival of the IL-7–dependent cell line 2E8 was measured in the presence of rhIL-7 (2,000 pg/mL) plus varying concentrations of sIL7Rα. No effects were seen at any time point using molar ratios of 128:1 and below. However, on day 12, increased survival was observed at sIL7Rα:IL-7 molar ratios of 256–512:1, which was diminished at higher molar ratios. ( B ) Using lower concentrations of rhIL-7 [250 pg/mL ( Left ) and 500 pg/mL ( Right )], survival of 2E8 from days 0–7 is shown with no cytokine, rhIL-7 alone, or sIL7Rα:rhIL7 molar ratio of 500:1. sIL7RΑ significantly increased survival on days 5 and 7 (* P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Soluble IL7R? potentiates IL-7 bioactivity and promotes autoimmunity

    doi: 10.1073/pnas.1222303110

    Figure Lengend Snippet: sIL7RΑ potentiates IL-7–mediated survival and diminishes consumption of IL-7. ( A ) Survival of the IL-7–dependent cell line 2E8 was measured in the presence of rhIL-7 (2,000 pg/mL) plus varying concentrations of sIL7Rα. No effects were seen at any time point using molar ratios of 128:1 and below. However, on day 12, increased survival was observed at sIL7Rα:IL-7 molar ratios of 256–512:1, which was diminished at higher molar ratios. ( B ) Using lower concentrations of rhIL-7 [250 pg/mL ( Left ) and 500 pg/mL ( Right )], survival of 2E8 from days 0–7 is shown with no cytokine, rhIL-7 alone, or sIL7Rα:rhIL7 molar ratio of 500:1. sIL7RΑ significantly increased survival on days 5 and 7 (* P

    Article Snippet: Human IL-7 levels in human plasma, mouse plasma, and culture supernatants were measured by high-sensitivity IL-7 ELISA kit according to the manufacturer’s instructions (Quantikine; R & D Systems).

    Techniques:

    sIL7Rα binds IL-7 but not TSLP. The binding kinetics for all of the cytokine/receptor interactions fit best to a two-step binding reaction model using two on-rate ( k 1 and k 2 ) and off-rate ( k -1 and k -2 ) constants. ( A–D ) Surface plasmon resonance sensorgrams are shown for each designated protein pair. Black lines represent raw data and red lines represent the global fitting analysis of the sensorgrams to a two-step binding reaction model using ClampXP. ( E ) Summary of binding affinities measured in A–D . Experiments were performed in triplicate with errors on the order of 5–10% for the rate constants.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Soluble IL7R? potentiates IL-7 bioactivity and promotes autoimmunity

    doi: 10.1073/pnas.1222303110

    Figure Lengend Snippet: sIL7Rα binds IL-7 but not TSLP. The binding kinetics for all of the cytokine/receptor interactions fit best to a two-step binding reaction model using two on-rate ( k 1 and k 2 ) and off-rate ( k -1 and k -2 ) constants. ( A–D ) Surface plasmon resonance sensorgrams are shown for each designated protein pair. Black lines represent raw data and red lines represent the global fitting analysis of the sensorgrams to a two-step binding reaction model using ClampXP. ( E ) Summary of binding affinities measured in A–D . Experiments were performed in triplicate with errors on the order of 5–10% for the rate constants.

    Article Snippet: Human IL-7 levels in human plasma, mouse plasma, and culture supernatants were measured by high-sensitivity IL-7 ELISA kit according to the manufacturer’s instructions (Quantikine; R & D Systems).

    Techniques: Binding Assay, SPR Assay

    sIL7Rα diminishes IL-7 clearance in vivo and increases IL-7–mediated homeostatic peripheral expansion. ( A ) On day 0, IL-7 −/− mice received one dose of rhIL-7 (5 μg) with or without sIL7Rα (100 μg) coinjected in the same syringe. A 10:1 molar ratio was chosen because of limited availability of recombinant sIL7Rα. Plasma levels were measured at 24 and 96 h. Mice injected with rhIL-7 plus sIL7Rα have higher plasma IL-7 levels after 24 h than those receiving rhIL-7 alone. ( B ) On day 0, mice received IL-7 with or without sIL7Rα, as in A above, plus 2 × 10 6 congenic lymph node cells. On day 8, mice injected with rhIL-7 plus sIL7Rα showed higher numbers of adoptively transferred CD4 + and CD8 + splenocytes compared with mice receiving rhIL-7 alone. Asterisks denote significant differences. These experiments were repeated once for two independent experiments with similar results ( n = 5 per group). ( C ) C57BL/6 mice ( n = 10/group) were immunized with MOG and then scored for EAE symptoms. PBS, rhIL-7, sIL7Rα, or rhIL-7 plus sIL7Rα was administered i.p. on days 9 and 15 postimmunization (arrows). Mice injected with rhIL-7 plus sIL7Rα showed increased EAE scores ( Left) , more rapid progression to EAE score 3 ( Center ), and higher overall disability as measured by area under the curve of EAE score over time per individual mouse ( Right ). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Soluble IL7R? potentiates IL-7 bioactivity and promotes autoimmunity

    doi: 10.1073/pnas.1222303110

    Figure Lengend Snippet: sIL7Rα diminishes IL-7 clearance in vivo and increases IL-7–mediated homeostatic peripheral expansion. ( A ) On day 0, IL-7 −/− mice received one dose of rhIL-7 (5 μg) with or without sIL7Rα (100 μg) coinjected in the same syringe. A 10:1 molar ratio was chosen because of limited availability of recombinant sIL7Rα. Plasma levels were measured at 24 and 96 h. Mice injected with rhIL-7 plus sIL7Rα have higher plasma IL-7 levels after 24 h than those receiving rhIL-7 alone. ( B ) On day 0, mice received IL-7 with or without sIL7Rα, as in A above, plus 2 × 10 6 congenic lymph node cells. On day 8, mice injected with rhIL-7 plus sIL7Rα showed higher numbers of adoptively transferred CD4 + and CD8 + splenocytes compared with mice receiving rhIL-7 alone. Asterisks denote significant differences. These experiments were repeated once for two independent experiments with similar results ( n = 5 per group). ( C ) C57BL/6 mice ( n = 10/group) were immunized with MOG and then scored for EAE symptoms. PBS, rhIL-7, sIL7Rα, or rhIL-7 plus sIL7Rα was administered i.p. on days 9 and 15 postimmunization (arrows). Mice injected with rhIL-7 plus sIL7Rα showed increased EAE scores ( Left) , more rapid progression to EAE score 3 ( Center ), and higher overall disability as measured by area under the curve of EAE score over time per individual mouse ( Right ). * P

    Article Snippet: Human IL-7 levels in human plasma, mouse plasma, and culture supernatants were measured by high-sensitivity IL-7 ELISA kit according to the manufacturer’s instructions (Quantikine; R & D Systems).

    Techniques: In Vivo, Mouse Assay, Recombinant, Injection

    sIL7Rα-mediated modulation of IL-7 signaling on human T cells leads to diminished IL-7 consumption and diminished SOCS1 and CD95 Induction. Human PBMCs were coincubated with rhIL-7 with or without sIL7Rα and then analyzed at the times shown. Molar ratios were as noted in A , whereas 500:1 sIL7Rα:rhIL7 molar ratio was used in B – D . ( A ) IL-7–induced STAT-5 phosphorylation was measured in gated CD4 + and CD8 + T cells after 15 min. Cells were serum-starved before IL-7 was added. No effect was seen at 50:1 molar ratio, incomplete inhibition was seen at 500:1 ratio (similar effects at 1 and 10 ng/mL rhIL-7), and complete inhibition was seen at 5,000:1 ratio. Controls using human serum albumin and rat IgG2a at similar concentrations showed no significant impact on STAT-5 signaling. ( B ) Modulation of IL-7–induced changes in CD127, CXCR4, and CD95 expression by sIL7Rα. Representative flow-cytometric histograms of CD127 expression at day 5 on CD4 + and CD8 + T cells are shown on the right. ( C ) IL-7 consumption by human PBMCs is diminished in the presence of sIL7Rα, as measured by ELISA. ( D ) SOCS1 levels are reduced in the presence of sIL7Rα after 24 h of incubation with IL-7. Relative quantity (RQ) of SOCS1/GAPDH mRNA expression compared with untreated cells was determined. Error bars represent 95% CI of triplicate experiments. A total of three independent experiments were performed using PBMCs from different donors with comparable results. Statistical significance shown (* P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Soluble IL7R? potentiates IL-7 bioactivity and promotes autoimmunity

    doi: 10.1073/pnas.1222303110

    Figure Lengend Snippet: sIL7Rα-mediated modulation of IL-7 signaling on human T cells leads to diminished IL-7 consumption and diminished SOCS1 and CD95 Induction. Human PBMCs were coincubated with rhIL-7 with or without sIL7Rα and then analyzed at the times shown. Molar ratios were as noted in A , whereas 500:1 sIL7Rα:rhIL7 molar ratio was used in B – D . ( A ) IL-7–induced STAT-5 phosphorylation was measured in gated CD4 + and CD8 + T cells after 15 min. Cells were serum-starved before IL-7 was added. No effect was seen at 50:1 molar ratio, incomplete inhibition was seen at 500:1 ratio (similar effects at 1 and 10 ng/mL rhIL-7), and complete inhibition was seen at 5,000:1 ratio. Controls using human serum albumin and rat IgG2a at similar concentrations showed no significant impact on STAT-5 signaling. ( B ) Modulation of IL-7–induced changes in CD127, CXCR4, and CD95 expression by sIL7Rα. Representative flow-cytometric histograms of CD127 expression at day 5 on CD4 + and CD8 + T cells are shown on the right. ( C ) IL-7 consumption by human PBMCs is diminished in the presence of sIL7Rα, as measured by ELISA. ( D ) SOCS1 levels are reduced in the presence of sIL7Rα after 24 h of incubation with IL-7. Relative quantity (RQ) of SOCS1/GAPDH mRNA expression compared with untreated cells was determined. Error bars represent 95% CI of triplicate experiments. A total of three independent experiments were performed using PBMCs from different donors with comparable results. Statistical significance shown (* P

    Article Snippet: Human IL-7 levels in human plasma, mouse plasma, and culture supernatants were measured by high-sensitivity IL-7 ELISA kit according to the manufacturer’s instructions (Quantikine; R & D Systems).

    Techniques: Inhibition, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Incubation