genechip human gene 1 0 st array  (Thermo Fisher)


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    Name:
    GeneChip Human Gene 1 0 ST Array
    Description:
    The GeneChip Human Gene 1 0 ST Array provides the most accurate sensitive and comprehensive measurement of protein coding and long intergenic non coding RNA transcripts We also offer the GeneChip Human Gene 2 0 ST Array which can measure differential expression of long intergenic non coding RNA transcripts
    Catalog Number:
    901085
    Price:
    None
    Applications:
    DNA & RNA Microarray Analysis|Microarray Analysis
    Category:
    Microarrays PCR Arrays
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    Structured Review

    Thermo Fisher genechip human gene 1 0 st array
    The GeneChip Human Gene 1 0 ST Array provides the most accurate sensitive and comprehensive measurement of protein coding and long intergenic non coding RNA transcripts We also offer the GeneChip Human Gene 2 0 ST Array which can measure differential expression of long intergenic non coding RNA transcripts
    https://www.bioz.com/result/genechip human gene 1 0 st array/product/Thermo Fisher
    Average 90 stars, based on 203 article reviews
    Price from $9.99 to $1999.99
    genechip human gene 1 0 st array - by Bioz Stars, 2020-01
    90/100 stars

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    Centrifugation:

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: To improve recovery from the columns, water or elution buffer was spun into the matrix at 50 g and left to stand for 4 minutes before a 16,000-g centrifugation. .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

    Amplification:

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: Amplified products were purified using the GeneChip cDNA Sample Cleanup Module (Affymetrix) with a 6,000-g centrifugation during the first two steps. .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

    Synthesized:

    Article Title: PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease
    Article Snippet: Materials Dulbecco’s phosphate-buffered saline (D-PBS) and TRIzol® reagent were purchased from Invitrogen (Carlsbad, CA, USA), the IsoPrep RNeasy total RNA kit was from Qiagen (Hilden, Germany), and the Affymetrix GeneChip® Human Gene 1.0 ST Array was from Affymetrix (Santa Clara, CA, USA). .. Quantitative reverse transcription (qRT)-PCR primers were designed using Primer3 (v.0.4.0) software, GenBank sequences and based on previous studies, and were synthesized by Pacific Science Co., Ltd. (Bangkok, Thailand).

    Quantitative RT-PCR:

    Article Title: PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease
    Article Snippet: Materials Dulbecco’s phosphate-buffered saline (D-PBS) and TRIzol® reagent were purchased from Invitrogen (Carlsbad, CA, USA), the IsoPrep RNeasy total RNA kit was from Qiagen (Hilden, Germany), and the Affymetrix GeneChip® Human Gene 1.0 ST Array was from Affymetrix (Santa Clara, CA, USA). .. Quantitative reverse transcription (qRT)-PCR primers were designed using Primer3 (v.0.4.0) software, GenBank sequences and based on previous studies, and were synthesized by Pacific Science Co., Ltd. (Bangkok, Thailand).

    Enzyme-linked Immunosorbent Assay:

    Article Title: PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease
    Article Snippet: Materials Dulbecco’s phosphate-buffered saline (D-PBS) and TRIzol® reagent were purchased from Invitrogen (Carlsbad, CA, USA), the IsoPrep RNeasy total RNA kit was from Qiagen (Hilden, Germany), and the Affymetrix GeneChip® Human Gene 1.0 ST Array was from Affymetrix (Santa Clara, CA, USA). .. Human HNP 1–3 enzyme-linked immunosorbent assay (ELISA) reagents were purchased from Hycult® Biotech (Uden, the Netherlands).

    Microarray:

    Article Title: Wnt/?-catenin signaling is a key downstream mediator of MET signaling in glioblastoma stem cells
    Article Snippet: Paragraph title: Microarray Analysis ... The global gene expressions of the METhigh/+ and METlow/− cells were analyzed using Affymetrix GeneChip Human Gene 1.0 ST Array.

    Article Title: Hypoxia-Inducible Factor 2α Mutation-Related Paragangliomas Classify as Discrete Pseudohypoxic Subcluster
    Article Snippet: GeneChip Human Gene 1.0 ST Array (Affymetrix) “Core” probe sets were used to perform “gene-level” probe set summarization, background subtraction, and quantile normalization using the RMA option in Expression Console 1.0 (Affymetrix). .. Differential expression analysis was done by significance analysis of microarray.

    Article Title: The oncogenic receptor ErbB2 modulates gemcitabine and irinotecan/SN-38 chemoresistance of human pancreatic cancer cells via hCNT1 transporter and multidrug-resistance associated protein MRP-2
    Article Snippet: Paragraph title: Gene Expression Omnibus microarray ... The dataset GSE28735 used Affymetrix GeneChip Human Gene 1.0 ST array.

    Article Title: Genome-wide methylation analysis identifies ELOVL5 as an epigenetic biomarker for the risk of type 2 diabetes mellitus
    Article Snippet: .. The data were from an mRNA expression microarray based on the Affymetrix GeneChip Human Gene 1.0 ST Array. ..

    Article Title: Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer. Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer
    Article Snippet: .. RNA were used for microarray analysis with a GeneChip Human Gene 1.0 ST Array (29 096 probe sets; Affymetrix, Santa Clara, CA, USA). .. The results from GeneChip analysis are available at the Gene Expression Omnibus Database ( http://www.ncbi.nlm.nih.gov/geo/ ) under Accession No. GSE75918.

    Article Title: Hepatitis C Virus-Induced Upregulation of MicroRNA miR-146a-5p in Hepatocytes Promotes Viral Infection and Deregulates Metabolic Pathways Associated with Liver Disease Pathogenesis
    Article Snippet: .. Genome-wide mRNA profiling in differentiated hepatocyte-like Huh7.5.1 cells overexpressing miR-146a-5p was performed using a GeneChip Human Gene 1.0 ST array (Affymetrix TSA) at the IGBMC Microarray and Sequencing Platform (Illkirch, France). .. Briefly, biotinylated single-stranded cDNA targets were prepared as described previously ( ).

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: Paragraph title: Microarray profiling ... Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

    Incubation:

    Article Title: A Novel Molecular Signature Identified by Systems Genetics Approach Predicts Prognosis in Oral Squamous Cell Carcinoma
    Article Snippet: SNP typing and transcriptome profiling Tissue was homogenized in a homogenizing buffer (50 mM Tris-HCl [pH 8.2], 20 mM EDTA, 10 mM NaCl and 1 mg/ml proteinase K) incubated at 56°C overnight. .. RNA from these preparations was used to probe the Affymetrix GeneChip Human Gene 1.0 ST Array according to the Affymetrix standard protocol.

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix). .. Arrays were normalized and probeset-level expression values calculated with R/Bioconductor's (v2.14) affy package using the rma() function [ ].

    Expressing:

    Article Title: Hypoxia-Inducible Factor 2α Mutation-Related Paragangliomas Classify as Discrete Pseudohypoxic Subcluster
    Article Snippet: .. GeneChip Human Gene 1.0 ST Array (Affymetrix) “Core” probe sets were used to perform “gene-level” probe set summarization, background subtraction, and quantile normalization using the RMA option in Expression Console 1.0 (Affymetrix). ..

    Article Title: Evaluation of copy number variation and gene expression in neurofibromatosis type-1-associated malignant peripheral nerve sheath tumours
    Article Snippet: In previous studies of NF1-MPNSTs, many genetic alterations, including copy number alterations and differential gene expression, have been identified but no specific molecular signature pathognomonic of malignant transformation has so far been defined [ - ]. .. This study employed the GeneChip Human Gene 1.0 ST array (Affymetrix, Santa Clara, USA).

    Article Title: Natriuretic peptides promote glucose uptake in a cGMP-dependent manner in human adipocytes
    Article Snippet: Gene microarrays From adipose tissue biopsy total RNA in Cohort 2, biotinylated complementary RNA was analyzed using the GeneChip Human Gene 1.0 ST Array (Affymetrix Inc., Santa Clara, CA). .. Data are deposited at the National Center for Biotechnology Information Gene Expression Omnibus (GEO; http://ncbi.nim.nih.gov/geo ) under the accession number GSE25402.

    Article Title: A Novel Molecular Signature Identified by Systems Genetics Approach Predicts Prognosis in Oral Squamous Cell Carcinoma
    Article Snippet: RNA from these preparations was used to probe the Affymetrix GeneChip Human Gene 1.0 ST Array according to the Affymetrix standard protocol. .. The data are publicly available in the Gene Expression Omnibus database under the accession number GSE25104.

    Article Title: The oncogenic receptor ErbB2 modulates gemcitabine and irinotecan/SN-38 chemoresistance of human pancreatic cancer cells via hCNT1 transporter and multidrug-resistance associated protein MRP-2
    Article Snippet: Paragraph title: Gene Expression Omnibus microarray ... The dataset GSE28735 used Affymetrix GeneChip Human Gene 1.0 ST array.

    Article Title: Fatty acid binding protein 7 may be a marker and therapeutic targets in clear cell renal cell carcinoma
    Article Snippet: .. Gene expression analysis was performed on the Genechip Human Gene 1.0 ST Array (Affymetrix®), according to the manufacturers’ instructions. .. The expression profiles were analyzed using an unsupervised hierarchical average linkage-clustering algorithm.

    Article Title: Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells
    Article Snippet: .. Gene Expression Analysis Gene expression profiles were analyzed by GeneChip Human Gene 1.0 ST Array (Affymetrix). .. DNA Methylation Analysis DNAm profiles were analyzed using the Infinium HumanMethylation450 BeadChip (Illumina), which addresses 485,577 CpG dinucleotides at a single-nucleotide resolution.

    Article Title: Genome-wide methylation analysis identifies ELOVL5 as an epigenetic biomarker for the risk of type 2 diabetes mellitus
    Article Snippet: .. The data were from an mRNA expression microarray based on the Affymetrix GeneChip Human Gene 1.0 ST Array. ..

    Article Title: Identification of CpG-SNPs associated with type 2 diabetes and differential DNA methylation in human pancreatic islets
    Article Snippet: .. Associations between type 2 diabetes CpG-SNPs that exhibit differential DNA methylation and alternative mRNA splicing The Gene Array Analyzer (GAA) [ ] was used together with mRNA expression data from the GeneChip Human Gene 1.0 ST array (Affymetrix) to test if type 2 diabetes CpG-SNPs that exhibit differential DNA methylation are associated with alternative splicing events in human islets. .. Each exon on the GeneChip Human Gene 1.0 ST array is covered by at least two probes, which is why the expression data from these arrays can be used to analyse alternative splicing events, as previously described [ ].

    Article Title: Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer. Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer
    Article Snippet: The results are available at the Gene Expression Omnibus database under Accession No. GSE75917. .. RNA were used for microarray analysis with a GeneChip Human Gene 1.0 ST Array (29 096 probe sets; Affymetrix, Santa Clara, CA, USA).

    Article Title: Implications of TGF? on Transcriptome and Cellular Biofunctions of Palatal Mesenchyme
    Article Snippet: .. TGFβ regulates differential expression of more than 2% of the palatal mesenchyme transcriptome We performed gene expression profiling on the Affymetrix GeneChip® Human Gene 1.0 ST Array representing 28,869 genes. .. We explored the alterations in gene expression in HEPM cells extracted from human embryonic palatal shelves in response to 10 ng/ml TGFβ1, TGFβ2, and TGFβ3 for 24 h. Statistically significant altered genes were filtered using dChip through normalization and modeling analysis.

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix). .. Arrays were normalized and probeset-level expression values calculated with R/Bioconductor's (v2.14) affy package using the rma() function [ ].

    Genome Wide:

    Article Title: A Novel Molecular Signature Identified by Systems Genetics Approach Predicts Prognosis in Oral Squamous Cell Carcinoma
    Article Snippet: DNA was hybridized to an Affymetrix Genome-wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA, USA) according to the manufacturer's protocol. .. RNA from these preparations was used to probe the Affymetrix GeneChip Human Gene 1.0 ST Array according to the Affymetrix standard protocol.

    Article Title: Hepatitis C Virus-Induced Upregulation of MicroRNA miR-146a-5p in Hepatocytes Promotes Viral Infection and Deregulates Metabolic Pathways Associated with Liver Disease Pathogenesis
    Article Snippet: .. Genome-wide mRNA profiling in differentiated hepatocyte-like Huh7.5.1 cells overexpressing miR-146a-5p was performed using a GeneChip Human Gene 1.0 ST array (Affymetrix TSA) at the IGBMC Microarray and Sequencing Platform (Illkirch, France). .. Briefly, biotinylated single-stranded cDNA targets were prepared as described previously ( ).

    Transformation Assay:

    Article Title: Evaluation of copy number variation and gene expression in neurofibromatosis type-1-associated malignant peripheral nerve sheath tumours
    Article Snippet: Identifying genes that are differentially expressed between benign and malignant tumours not only promises to improve our understanding of the process of malignant transformation but should also aid in identifying potential therapeutic targets. .. This study employed the GeneChip Human Gene 1.0 ST array (Affymetrix, Santa Clara, USA).

    Hybridization:

    Article Title: Hepatitis C Virus-Induced Upregulation of MicroRNA miR-146a-5p in Hepatocytes Promotes Viral Infection and Deregulates Metabolic Pathways Associated with Liver Disease Pathogenesis
    Article Snippet: Genome-wide mRNA profiling in differentiated hepatocyte-like Huh7.5.1 cells overexpressing miR-146a-5p was performed using a GeneChip Human Gene 1.0 ST array (Affymetrix TSA) at the IGBMC Microarray and Sequencing Platform (Illkirch, France). .. Following fragmentation and end labeling, hybridization of 2.07 μg of cDNAs was performed using a GeneChip fluidics station 450 instrument (Affymetrix) for 16 h at 45°C.

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix). .. Arrays were normalized and probeset-level expression values calculated with R/Bioconductor's (v2.14) affy package using the rma() function [ ].

    Transfection:

    Article Title: Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer. Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer
    Article Snippet: 2.4 Microarray analysis MCF‐7 cells were transfected with control or DYRK2 siRNA. .. RNA were used for microarray analysis with a GeneChip Human Gene 1.0 ST Array (29 096 probe sets; Affymetrix, Santa Clara, CA, USA).

    Generated:

    Article Title: Evaluation of copy number variation and gene expression in neurofibromatosis type-1-associated malignant peripheral nerve sheath tumours
    Article Snippet: This study employed the GeneChip Human Gene 1.0 ST array (Affymetrix, Santa Clara, USA). .. Additionally, this array uses a labelling protocol that generates biotinylated sense strand DNA instead of complementary RNA (cRNA), thereby yielding DNA-DNA duplexes, which are more specific than the RNA-DNA duplexes generated using standard protocols [ ].

    Sequencing:

    Article Title: Hepatitis C Virus-Induced Upregulation of MicroRNA miR-146a-5p in Hepatocytes Promotes Viral Infection and Deregulates Metabolic Pathways Associated with Liver Disease Pathogenesis
    Article Snippet: .. Genome-wide mRNA profiling in differentiated hepatocyte-like Huh7.5.1 cells overexpressing miR-146a-5p was performed using a GeneChip Human Gene 1.0 ST array (Affymetrix TSA) at the IGBMC Microarray and Sequencing Platform (Illkirch, France). .. Briefly, biotinylated single-stranded cDNA targets were prepared as described previously ( ).

    Binding Assay:

    Article Title: Genome-wide methylation analysis identifies ELOVL5 as an epigenetic biomarker for the risk of type 2 diabetes mellitus
    Article Snippet: The data were from an mRNA expression microarray based on the Affymetrix GeneChip Human Gene 1.0 ST Array. .. LASAGNA-Search 2.0 showed no evidence for transcription factor binding sites positionally overlapping with CpG sites in the promoter region of ELOVL5 (data not shown) .

    Isolation:

    Article Title: A Novel Molecular Signature Identified by Systems Genetics Approach Predicts Prognosis in Oral Squamous Cell Carcinoma
    Article Snippet: Total RNA was isolated from tissue using the Qiagen RNeasy Mini Kit (Qiagen, Germantown, MD, USA) according to the manufacturer's protocol. .. RNA from these preparations was used to probe the Affymetrix GeneChip Human Gene 1.0 ST Array according to the Affymetrix standard protocol.

    Labeling:

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: The cDNA was then fragmented by uracil DNA glycosylase and apurinic/apyrimidic endonuclease 1 and biotin-labeled with terminal deoxynucleotidyl transferase using the GeneChip WT Terminal labeling kit (Affymetrix). .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

    Purification:

    Article Title: A Novel Molecular Signature Identified by Systems Genetics Approach Predicts Prognosis in Oral Squamous Cell Carcinoma
    Article Snippet: DNA was purified by phenol/chloroform extraction and sodium acetate/ethanol precipitation. .. RNA from these preparations was used to probe the Affymetrix GeneChip Human Gene 1.0 ST Array according to the Affymetrix standard protocol.

    Article Title: Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer. Downregulation of dual‐specificity tyrosine‐regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin‐dependent kinase 14 expression in breast cancer
    Article Snippet: Total RNA was purified from the pSuper control cells and shDYRK2 cells. .. RNA were used for microarray analysis with a GeneChip Human Gene 1.0 ST Array (29 096 probe sets; Affymetrix, Santa Clara, CA, USA).

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: Amplified products were purified using the GeneChip cDNA Sample Cleanup Module (Affymetrix) with a 6,000-g centrifugation during the first two steps. .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

    Chromatin Immunoprecipitation:

    Article Title: Wnt/?-catenin signaling is a key downstream mediator of MET signaling in glioblastoma stem cells
    Article Snippet: The global gene expressions of the METhigh/+ and METlow/− cells were analyzed using Affymetrix GeneChip Human Gene 1.0 ST Array. .. Total RNA was processed and hybridized to the array chip according to the manufacturer's manual (Affymetrix, USA).

    Software:

    Article Title: PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease
    Article Snippet: Materials Dulbecco’s phosphate-buffered saline (D-PBS) and TRIzol® reagent were purchased from Invitrogen (Carlsbad, CA, USA), the IsoPrep RNeasy total RNA kit was from Qiagen (Hilden, Germany), and the Affymetrix GeneChip® Human Gene 1.0 ST Array was from Affymetrix (Santa Clara, CA, USA). .. Quantitative reverse transcription (qRT)-PCR primers were designed using Primer3 (v.0.4.0) software, GenBank sequences and based on previous studies, and were synthesized by Pacific Science Co., Ltd. (Bangkok, Thailand).

    Article Title: The oncogenic receptor ErbB2 modulates gemcitabine and irinotecan/SN-38 chemoresistance of human pancreatic cancer cells via hCNT1 transporter and multidrug-resistance associated protein MRP-2
    Article Snippet: Data were analysed using GEO2R software. .. The dataset GSE28735 used Affymetrix GeneChip Human Gene 1.0 ST array.

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix). .. Arrays were normalized and probeset-level expression values calculated with R/Bioconductor's (v2.14) affy package using the rma() function [ ].

    Spectrophotometry:

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: The quantity and purity of the cRNA and cDNA produced during the first and second rounds were evaluated using a NanoDrop ND-1000 spectrophotometer (Nanodrop Technologies). .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

    DNA Methylation Assay:

    Article Title: Genome-wide methylation analysis identifies ELOVL5 as an epigenetic biomarker for the risk of type 2 diabetes mellitus
    Article Snippet: ELOVL5 is correlated with gene expression in human pancreatic islets To investigate the relationship between DNA methylation and gene expression, we tested expression profiles using publicly accessible resources (GEO accession number: GSE38642) in the Gene Expression Omnibus (GEO) database. .. The data were from an mRNA expression microarray based on the Affymetrix GeneChip Human Gene 1.0 ST Array.

    Article Title: Identification of CpG-SNPs associated with type 2 diabetes and differential DNA methylation in human pancreatic islets
    Article Snippet: .. Associations between type 2 diabetes CpG-SNPs that exhibit differential DNA methylation and alternative mRNA splicing The Gene Array Analyzer (GAA) [ ] was used together with mRNA expression data from the GeneChip Human Gene 1.0 ST array (Affymetrix) to test if type 2 diabetes CpG-SNPs that exhibit differential DNA methylation are associated with alternative splicing events in human islets. .. Each exon on the GeneChip Human Gene 1.0 ST array is covered by at least two probes, which is why the expression data from these arrays can be used to analyse alternative splicing events, as previously described [ ].

    Produced:

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: The quantity and purity of the cRNA and cDNA produced during the first and second rounds were evaluated using a NanoDrop ND-1000 spectrophotometer (Nanodrop Technologies). .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

    End Labeling:

    Article Title: Hepatitis C Virus-Induced Upregulation of MicroRNA miR-146a-5p in Hepatocytes Promotes Viral Infection and Deregulates Metabolic Pathways Associated with Liver Disease Pathogenesis
    Article Snippet: Genome-wide mRNA profiling in differentiated hepatocyte-like Huh7.5.1 cells overexpressing miR-146a-5p was performed using a GeneChip Human Gene 1.0 ST array (Affymetrix TSA) at the IGBMC Microarray and Sequencing Platform (Illkirch, France). .. Following fragmentation and end labeling, hybridization of 2.07 μg of cDNAs was performed using a GeneChip fluidics station 450 instrument (Affymetrix) for 16 h at 45°C.

    Staining:

    Article Title: Mesenchymal precursor cells maintain the differentiation and proliferation potentials of breast epithelial cells
    Article Snippet: Following hybridization, non-specifically bound nucleotides were removed by washing and specifically bound target detected using a GeneChip Hybridization, Wash and Stain kit and a GeneChip Fluidics Station 450 (Affymetrix). .. Hybridization was carried out with 5 μg of biotinylated target, which was incubated with a GeneChip human Gene 1.0 ST Array (Affymetrix) at 45°C for 16 h. The arrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix) and CEL files acquired using GeneChip Command Console Software (Affymetrix).

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  • 90
    Thermo Fisher genechip human gene 1 0 st array
    Genechip Human Gene 1 0 St Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genechip human gene 1 0 st array/product/Thermo Fisher
    Average 90 stars, based on 448 article reviews
    Price from $9.99 to $1999.99
    genechip human gene 1 0 st array - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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