Journal: Biotechnology Reports

Article Title: A fusion protein designed for soluble expression, rapid purification, and enhanced stability of parasporin-2 with potential therapeutic applications

doi: 10.1016/j.btre.2024.e00851

Figure Lengend Snippet: Cell viability (%) of Hs68 (A) Jurkat (B) and MOLT-4 (C) cells after being treated with His 6 -fPS2, MBP-fPS2, and MBP-tPS2 at different concentrations. The proteinase K-treated and non-proteinase K-treated samples are denoted as + ProK and – ProK, respectively. Cell viability (%) of Jurkat (D) and MOLT-4 (E) in response to non-proteinase K-treated MBP-fPS2 and MBP-tPS2 treatments at different concentrations. After 24 h of incubation with various concentrations of protein samples, the percentage of cell viability was determined by MTT assay by comparing the absorbance of each sample to that of the negative control (PBS, pH 7.4). All data are presented as mean ± SD from the triplicate analyses. Statistical significance was analyzed using two-way ANOVA and annotated as follows: **** p < 0.0001, *** p < 0.001, ns (no significance) p > 0.9.

Article Snippet: Human foreskin fibroblast cell line Hs68 (ATCC CRL-1635), T-acute lymphoblastic leukemia cells lines: MOLT-4 (ATCC CRL-1582) and Jurkat (ATCC TIB-152T) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation, MTT Assay, Negative Control

Journal: Molecules

Article Title: From Ethnobotany to Biotechnology: Wound Healing and Anti-Inflammatory Properties of Sedum telephium L. In Vitro Cultures

doi: 10.3390/molecules29112472

Figure Lengend Snippet: Panel ( A ). Wound healing in HFF1 cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.

Article Snippet: The human foreskin fibroblasts 1 (HFF1) cell line was purchased from the American Type Culture Collection (ATCC, LGC Standards S.r.l., Sesto San Giovanni, Italy) and routinely cultured in DMEM supplemented with 1% ( vol / vol ) penicillin and streptomycin, 2 mM L-glutamine, and 10% ( vol / vol ) heat-inactivated FBS (all purchased from Life Technologies).

Techniques: Microscopy, Software, Incubation