Journal: bioRxiv
Article Title: Endothelial FABP4 constitutes the majority of basal circulating hormone levels and regulates lipolysis-driven insulin secretion
doi: 10.1101/2022.10.13.511807
Figure Lengend Snippet: ( A) Top panel: Plasma FABP4 levels in WT, Adipo-KO, Endo-KO, and Total-KO lean male mice, after 6h daytime food withdrawal. Data are pooled from samples from 9 experiments. ****p<0.0001, **p<0.01. Bottom panel: Immunoblots of perirenal and mesenteric adipose FABP4 protein expression (same images as in and S2A), for comparison with plasma levels. ( B ) Plasma FABP4 levels in 6h WT vs. Myeloid-KO mice. Data are pooled from samples from 2 experiments. ( C ) Plasma FABP5 levels in WT, Adipo-KO, Endo-KO and Total-KO mice. *p<0.05. Data in 3A, 3C, and 3D were analyzed by one-way ANOVA, followed by Tukey’s multiple comparison test. Data in 3B were analyzed by unpaired t-test. Data are presented as mean ± SEM. ND: No signal detected.
Article Snippet: Plasma and media FABP5, insulin, von Willebrand factor (vWF), and endothelin-1 (ET-1) levels were determined by commercially available ELISA (CircuLex Mouse FABP5/-E-FABP/mal1 ELISA kit, cat# CY-8056; Alpco Mouse Ultrasensitive Insulin ELISA, cat# 80-INSMSU-E01 or Crystal Chem Ultra Sensitive Mouse Insulin ELISA, cat# 90080; Human vWF-A2 DuoSet ELISA, R&D Systems, cat # DY2764-05; Human Endothelin-1 QuantiGlo ELISA Kit, R&D Systems, cat # QET00B).
Techniques: Western Blot, Expressing, Comparison