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Elabscience human cygb elisa kit
Human Cygb Elisa Kit, supplied by Elabscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cygb elisa kit/product/Elabscience
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human cygb elisa kit - by Bioz Stars, 2020-08
93/100 stars

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Modification:

Article Title: Role of Hypoxia Inducible Factor-1 Alpha (HIF-1α) in Cytoglobin Expression and Fibroblast Proliferation of Keloids
Article Snippet: .. Material for this studies were as follow: Dulbecco’s modified Eagles medium (DMEM) low-glucose (Gibco, Fisher Scientific, UK), 1% penicillin-streptomycin (Sigma-Aldrich, USA), phosphate buffered saline/PBS (Sigma-Aldrich, USA), 1% amphotericin (Biosera, UK), 10% fetal bovine serum/FBS (Gibco, Thermo Scientific, UK ), 24-well culture plate for primary fibroblast culture (Costar, Corning, USA), Ibuprofen (Sigma-Aldrich, USA) for HIF-1α inhibition; Tripure Isolation reagent (Sigma-Aldrich, USA) for total RNA isolation; Primers of qRT PCR for HIF-1α were 5′-TGATGACCAGCAACTTGAGG-3′ (forward) and 5′-TTGATTGAGTGCAGGGTCAG-3′(reverse); while primers for Cygb were 5′-CAGTTCAAGCACATGGAGGA-3′ (forward) and 5′-GTGGGAAGTCACTGGCAAAT-3′ (reverse); 18s rRNA primer as housekeeping gene were 5′-AAACGGCTACCACATCCAAG-3′ (forward) and 5′-CCTCCAATGGATCCTCGTTA-3′ (reverse); cell extraction buffer (Thermo Fisher Scientific, USA) to isolate total protein; bovine serum albumin/BSA (Sigma-Aldrich, USA) and the material for ELISA kit were human HIF-1α ELISA kit (Elabscience, UK) and human Cygb ELISA kit (Elabscience, UK), trypan blue (Sigma-Aldrich, USA) for determination of fibroblast proliferation. ..

Isolation:

Article Title: Role of Hypoxia Inducible Factor-1 Alpha (HIF-1α) in Cytoglobin Expression and Fibroblast Proliferation of Keloids
Article Snippet: .. Material for this studies were as follow: Dulbecco’s modified Eagles medium (DMEM) low-glucose (Gibco, Fisher Scientific, UK), 1% penicillin-streptomycin (Sigma-Aldrich, USA), phosphate buffered saline/PBS (Sigma-Aldrich, USA), 1% amphotericin (Biosera, UK), 10% fetal bovine serum/FBS (Gibco, Thermo Scientific, UK ), 24-well culture plate for primary fibroblast culture (Costar, Corning, USA), Ibuprofen (Sigma-Aldrich, USA) for HIF-1α inhibition; Tripure Isolation reagent (Sigma-Aldrich, USA) for total RNA isolation; Primers of qRT PCR for HIF-1α were 5′-TGATGACCAGCAACTTGAGG-3′ (forward) and 5′-TTGATTGAGTGCAGGGTCAG-3′(reverse); while primers for Cygb were 5′-CAGTTCAAGCACATGGAGGA-3′ (forward) and 5′-GTGGGAAGTCACTGGCAAAT-3′ (reverse); 18s rRNA primer as housekeeping gene were 5′-AAACGGCTACCACATCCAAG-3′ (forward) and 5′-CCTCCAATGGATCCTCGTTA-3′ (reverse); cell extraction buffer (Thermo Fisher Scientific, USA) to isolate total protein; bovine serum albumin/BSA (Sigma-Aldrich, USA) and the material for ELISA kit were human HIF-1α ELISA kit (Elabscience, UK) and human Cygb ELISA kit (Elabscience, UK), trypan blue (Sigma-Aldrich, USA) for determination of fibroblast proliferation. ..

Inhibition:

Article Title: Role of Hypoxia Inducible Factor-1 Alpha (HIF-1α) in Cytoglobin Expression and Fibroblast Proliferation of Keloids
Article Snippet: .. Material for this studies were as follow: Dulbecco’s modified Eagles medium (DMEM) low-glucose (Gibco, Fisher Scientific, UK), 1% penicillin-streptomycin (Sigma-Aldrich, USA), phosphate buffered saline/PBS (Sigma-Aldrich, USA), 1% amphotericin (Biosera, UK), 10% fetal bovine serum/FBS (Gibco, Thermo Scientific, UK ), 24-well culture plate for primary fibroblast culture (Costar, Corning, USA), Ibuprofen (Sigma-Aldrich, USA) for HIF-1α inhibition; Tripure Isolation reagent (Sigma-Aldrich, USA) for total RNA isolation; Primers of qRT PCR for HIF-1α were 5′-TGATGACCAGCAACTTGAGG-3′ (forward) and 5′-TTGATTGAGTGCAGGGTCAG-3′(reverse); while primers for Cygb were 5′-CAGTTCAAGCACATGGAGGA-3′ (forward) and 5′-GTGGGAAGTCACTGGCAAAT-3′ (reverse); 18s rRNA primer as housekeeping gene were 5′-AAACGGCTACCACATCCAAG-3′ (forward) and 5′-CCTCCAATGGATCCTCGTTA-3′ (reverse); cell extraction buffer (Thermo Fisher Scientific, USA) to isolate total protein; bovine serum albumin/BSA (Sigma-Aldrich, USA) and the material for ELISA kit were human HIF-1α ELISA kit (Elabscience, UK) and human Cygb ELISA kit (Elabscience, UK), trypan blue (Sigma-Aldrich, USA) for determination of fibroblast proliferation. ..

Quantitative RT-PCR:

Article Title: Role of Hypoxia Inducible Factor-1 Alpha (HIF-1α) in Cytoglobin Expression and Fibroblast Proliferation of Keloids
Article Snippet: .. Material for this studies were as follow: Dulbecco’s modified Eagles medium (DMEM) low-glucose (Gibco, Fisher Scientific, UK), 1% penicillin-streptomycin (Sigma-Aldrich, USA), phosphate buffered saline/PBS (Sigma-Aldrich, USA), 1% amphotericin (Biosera, UK), 10% fetal bovine serum/FBS (Gibco, Thermo Scientific, UK ), 24-well culture plate for primary fibroblast culture (Costar, Corning, USA), Ibuprofen (Sigma-Aldrich, USA) for HIF-1α inhibition; Tripure Isolation reagent (Sigma-Aldrich, USA) for total RNA isolation; Primers of qRT PCR for HIF-1α were 5′-TGATGACCAGCAACTTGAGG-3′ (forward) and 5′-TTGATTGAGTGCAGGGTCAG-3′(reverse); while primers for Cygb were 5′-CAGTTCAAGCACATGGAGGA-3′ (forward) and 5′-GTGGGAAGTCACTGGCAAAT-3′ (reverse); 18s rRNA primer as housekeeping gene were 5′-AAACGGCTACCACATCCAAG-3′ (forward) and 5′-CCTCCAATGGATCCTCGTTA-3′ (reverse); cell extraction buffer (Thermo Fisher Scientific, USA) to isolate total protein; bovine serum albumin/BSA (Sigma-Aldrich, USA) and the material for ELISA kit were human HIF-1α ELISA kit (Elabscience, UK) and human Cygb ELISA kit (Elabscience, UK), trypan blue (Sigma-Aldrich, USA) for determination of fibroblast proliferation. ..

Enzyme-linked Immunosorbent Assay:

Article Title: Role of Hypoxia Inducible Factor-1 Alpha (HIF-1α) in Cytoglobin Expression and Fibroblast Proliferation of Keloids
Article Snippet: .. Material for this studies were as follow: Dulbecco’s modified Eagles medium (DMEM) low-glucose (Gibco, Fisher Scientific, UK), 1% penicillin-streptomycin (Sigma-Aldrich, USA), phosphate buffered saline/PBS (Sigma-Aldrich, USA), 1% amphotericin (Biosera, UK), 10% fetal bovine serum/FBS (Gibco, Thermo Scientific, UK ), 24-well culture plate for primary fibroblast culture (Costar, Corning, USA), Ibuprofen (Sigma-Aldrich, USA) for HIF-1α inhibition; Tripure Isolation reagent (Sigma-Aldrich, USA) for total RNA isolation; Primers of qRT PCR for HIF-1α were 5′-TGATGACCAGCAACTTGAGG-3′ (forward) and 5′-TTGATTGAGTGCAGGGTCAG-3′(reverse); while primers for Cygb were 5′-CAGTTCAAGCACATGGAGGA-3′ (forward) and 5′-GTGGGAAGTCACTGGCAAAT-3′ (reverse); 18s rRNA primer as housekeeping gene were 5′-AAACGGCTACCACATCCAAG-3′ (forward) and 5′-CCTCCAATGGATCCTCGTTA-3′ (reverse); cell extraction buffer (Thermo Fisher Scientific, USA) to isolate total protein; bovine serum albumin/BSA (Sigma-Aldrich, USA) and the material for ELISA kit were human HIF-1α ELISA kit (Elabscience, UK) and human Cygb ELISA kit (Elabscience, UK), trypan blue (Sigma-Aldrich, USA) for determination of fibroblast proliferation. ..

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    Elabscience human cygb elisa kit
    Human Cygb Elisa Kit, supplied by Elabscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cygb elisa kit/product/Elabscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human cygb elisa kit - by Bioz Stars, 2020-08
    93/100 stars
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