human cot 1 dna  (Millipore)


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    Name:
    COT Human DNA
    Description:
    The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements These interspersed repetitive sequences IRS such as SINEs small interspersed repetitive elements e g Alu elements and LINEs large interspersed repetitive elements e g L1 elements are distributed ubiquitously throughout the genome COT Human DNA is prepared from human placental DNA by shearing denaturing and reannealing under conditions that enrich these repetitive elements
    Catalog Number:
    11581074001
    Price:
    None
    Applications:
    COT Human DNA is used in chromosome in situ suppression (CISS) hybridization. Cosmid or YAC probes contain repetitive elements that result in monospecific hybridization signals distributed over the entire chromosome. To enable specific hybridization to the chromosomal target site, the probe is denatured together with an excess of unlabeled COT Human DNA as a competitor. COT Human DNA can be used to suppress nonspecific hybridization to human repetitive sequences in microarray analysis, and in filter and in fluorescent in situ hybridization experiments.
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    Structured Review

    Millipore human cot 1 dna
    COT Human DNA
    The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements These interspersed repetitive sequences IRS such as SINEs small interspersed repetitive elements e g Alu elements and LINEs large interspersed repetitive elements e g L1 elements are distributed ubiquitously throughout the genome COT Human DNA is prepared from human placental DNA by shearing denaturing and reannealing under conditions that enrich these repetitive elements
    https://www.bioz.com/result/human cot 1 dna/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    human cot 1 dna - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Metagenomic Profiling: Microarray Analysis of an Environmental Genomic Library
    Article Snippet: .. Test DNA and reference DNA were combined with 40 μg of human Cot-1 DNA and 100 μg of salmon sperm genomic DNA and reduced to a volume of 5 μl by using Microcon YM-30 concentrators (Millipore); 20 μl of 1.25× unihyb hybridization buffer (Telechem) was added to the target DNA mixture, and the target was preannealed to blocking DNA by boiling the preparation for 1.5 min, followed by 30 min of incubation at 37°C. ..

    Blocking Assay:

    Article Title: Metagenomic Profiling: Microarray Analysis of an Environmental Genomic Library
    Article Snippet: .. Test DNA and reference DNA were combined with 40 μg of human Cot-1 DNA and 100 μg of salmon sperm genomic DNA and reduced to a volume of 5 μl by using Microcon YM-30 concentrators (Millipore); 20 μl of 1.25× unihyb hybridization buffer (Telechem) was added to the target DNA mixture, and the target was preannealed to blocking DNA by boiling the preparation for 1.5 min, followed by 30 min of incubation at 37°C. ..

    Labeling:

    Article Title: Deciphering the Contribution of Human Meningothelial Cells to the Inflammatory and Antimicrobial Response at the Meninges
    Article Snippet: .. Labeled aRNA, in a maximum volume of 4 μl containing 40 pmol of dye equivalents, was mixed with 1 μg (1 μl) human Cot-I DNA (Sigma) and 100 μl 1× Schott hybridization buffer (Schott) and hybridized to microarrays containing 30,000 human gene-specific probes (Human 30K OciChip [A, B, and C]; Ocimum Biosolutions) and printed onto Schott Nexterion A+ APS-coated glass slides by the Post-genomics Technology Facility (University of Nottingham, Nottingham, United Kingdom). .. Hybridizations were performed on a HS 4800 hybridization station (Tecan) for 16 h at 50°C with moderate agitation.

    Hybridization:

    Article Title: Metagenomic Profiling: Microarray Analysis of an Environmental Genomic Library
    Article Snippet: .. Test DNA and reference DNA were combined with 40 μg of human Cot-1 DNA and 100 μg of salmon sperm genomic DNA and reduced to a volume of 5 μl by using Microcon YM-30 concentrators (Millipore); 20 μl of 1.25× unihyb hybridization buffer (Telechem) was added to the target DNA mixture, and the target was preannealed to blocking DNA by boiling the preparation for 1.5 min, followed by 30 min of incubation at 37°C. ..

    Article Title: Population-specific gene expression responses to hybridization between farm and wild Atlantic salmon
    Article Snippet: .. The cDNA was hybridized to the microarrays in a formamide-based buffer (25% formamide, 4X SSC, 0.5% SDS, 2X Denhardt's solution) with competitor DNA [LNA dT bloker (Genisphere, Hatfield, Pennsylvania, US), human COT-1 DNA (Sigma)] for 16 h in hybridization chambers (Corning, New York, US) placed in a 51°C water bath. .. The arrays were washed once for 5 min at 45°C in 2X SSC, 0.1% SDS, twice for 3 min in 2X SSC, 0.1% SDS at room temperature (RT), twice for 3 min in 1X SSC at RT, twice for 3 min in 0.1X SSC at RT, and dried by centrifugation.

    Article Title: Deciphering the Contribution of Human Meningothelial Cells to the Inflammatory and Antimicrobial Response at the Meninges
    Article Snippet: .. Labeled aRNA, in a maximum volume of 4 μl containing 40 pmol of dye equivalents, was mixed with 1 μg (1 μl) human Cot-I DNA (Sigma) and 100 μl 1× Schott hybridization buffer (Schott) and hybridized to microarrays containing 30,000 human gene-specific probes (Human 30K OciChip [A, B, and C]; Ocimum Biosolutions) and printed onto Schott Nexterion A+ APS-coated glass slides by the Post-genomics Technology Facility (University of Nottingham, Nottingham, United Kingdom). .. Hybridizations were performed on a HS 4800 hybridization station (Tecan) for 16 h at 50°C with moderate agitation.

    Article Title: HiCTMap: Detection and analysis of chromosome territory structure and position by high-throughput imaging
    Article Snippet: .. Probe mix for chromosome paint probes consisting each of 300 ng DNA, 30 μg salmon sperm DNA (Ambion, Cat # AM9680), and 1 μg human COT1 Human DNA (Sigma-Aldrich Roche, Cat # 11581074001) per well was ethanol precipitated at −80 °C for 25 min and re-suspended in 12 μL of hybridization buffer per well (50% deionized formamide, pH7 (Ambion, Cat # AM9342), 20% dextran sulfate (Milipore, Cat # S4030), and 2X SSC (KSD Scientific, Cat # B3821-1000). .. Fixed cells were permeabilized for 20 min in 0.5% Triton X-100 (Sigma-Aldrich, Cat # T9234-500ML) in DPBS, washed in 0.05% Triton X-100 in DPBS, incubated for 10 min in 0.1 N HCl, neutralized for 5 min in 2X SSC, and equilibrated for at least 30 min in 50% formamide in 2X SSC prior to probe addition.

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  • 92
    Millipore human cot 1 dna
    Human Cot 1 Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cot 1 dna/product/Millipore
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    human cot 1 dna - by Bioz Stars, 2020-09
    92/100 stars
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