human brain mrna  (TaKaRa)

 
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    Name:
    Human Brain Poly A RNA
    Description:
    Our poly A RNA is enriched for mRNA transcripts with two rounds of oligo dT cellulose purification The mRNA is isolated from our total RNA collection which is meticulously prepared to high quality using our proprietary modified guanidinium thiocyanate method We offer an extensive selection of poly A RNA from the human CNS including tissues of the brain and spinal cord
    Catalog Number:
    636102
    Price:
    None
    Size:
    5 ug
    Category:
    Brain CNS Poly A RNA human Purified total RNA and mRNA cDNA synthesis
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    Structured Review

    TaKaRa human brain mrna
    ZNF135 is expressed in fetal and adult human brain. <t>rtPCR</t> products of the expected size for ZNF135 from human adult (lane 2) and fetal (lane 3) whole brain <t>mRNA.</t> Lanes 4 and 5 were duplicate reactions without adding primers. Lane 1 is MW marker.
    Our poly A RNA is enriched for mRNA transcripts with two rounds of oligo dT cellulose purification The mRNA is isolated from our total RNA collection which is meticulously prepared to high quality using our proprietary modified guanidinium thiocyanate method We offer an extensive selection of poly A RNA from the human CNS including tissues of the brain and spinal cord
    https://www.bioz.com/result/human brain mrna/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human brain mrna - by Bioz Stars, 2020-09
    92/100 stars

    Images

    1) Product Images from "Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms"

    Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms

    Journal: Neuroreport

    doi: 10.1097/WNR.0000000000000754

    ZNF135 is expressed in fetal and adult human brain. rtPCR products of the expected size for ZNF135 from human adult (lane 2) and fetal (lane 3) whole brain mRNA. Lanes 4 and 5 were duplicate reactions without adding primers. Lane 1 is MW marker.
    Figure Legend Snippet: ZNF135 is expressed in fetal and adult human brain. rtPCR products of the expected size for ZNF135 from human adult (lane 2) and fetal (lane 3) whole brain mRNA. Lanes 4 and 5 were duplicate reactions without adding primers. Lane 1 is MW marker.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker

    Related Articles

    RNA Sequencing Assay:

    Article Title: ACNP 55th Annual Meeting: Poster Session II December 6, 2016
    Article Snippet: .. Identify GAD1 transcripts: To find potential splicing variants, RNA sequencing was performed on two commercial (Clontech) pooled poly A+ RNA samples (human fetal brain poly A+ RNA and human adult brain poly A+ RNA). ..

    Synthesized:

    Article Title: Limited Inhibitory Effects of Oseltamivir and Zanamivir on Human Sialidases ▿
    Article Snippet: .. To obtain the NEU2 cDNA, the first-strand cDNAs were synthesized from poly(A)+ RNA from human brain (Clontech) using oligo(dT)12-18 primers and murine leukemia virus reverse transcriptase (SuperscriptII reverse transcriptase; Invitrogen) and applied as templates for the PCR described previously ( ). .. To cover the entire coding sequence, the cDNA was amplified with the two primer pairs with EcoRI sites (5′-ATGGCGTCCCTTCCTGTCCTG-3′, forward, and 5′-TCACTGAGGCAGGTACTCAGC-3′) using LA Taq polymerase (Takara), subcloned into pBluescript, sequenced, and cloned into the expression vector.

    Northern Blot:

    Article Title: PRAJA is overexpressed in glioblastoma and contributes to neural precursor development
    Article Snippet: .. Northern blot analysis Northern blots with 2 μ g poly-A(+) mRNA from human brain (Clontech, USA) were probed with 32 P-labeled Praja Clone CH7 [ ] insert antisense strand using ExpressHyb hybridization solution (Clontech, USA) at 68°C. .. A 32 P-labeled β-actin probe supplied with the Northern blots was used as a control to confirm that normalized RNA levels were present in each lane (Clontech).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
    Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

    DNA Sequencing:

    Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
    Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

    Expressing:

    Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
    Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

    Polymerase Chain Reaction:

    Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
    Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

    Article Title: Limited Inhibitory Effects of Oseltamivir and Zanamivir on Human Sialidases ▿
    Article Snippet: .. To obtain the NEU2 cDNA, the first-strand cDNAs were synthesized from poly(A)+ RNA from human brain (Clontech) using oligo(dT)12-18 primers and murine leukemia virus reverse transcriptase (SuperscriptII reverse transcriptase; Invitrogen) and applied as templates for the PCR described previously ( ). .. To cover the entire coding sequence, the cDNA was amplified with the two primer pairs with EcoRI sites (5′-ATGGCGTCCCTTCCTGTCCTG-3′, forward, and 5′-TCACTGAGGCAGGTACTCAGC-3′) using LA Taq polymerase (Takara), subcloned into pBluescript, sequenced, and cloned into the expression vector.

    Hybridization:

    Article Title: PRAJA is overexpressed in glioblastoma and contributes to neural precursor development
    Article Snippet: .. Northern blot analysis Northern blots with 2 μ g poly-A(+) mRNA from human brain (Clontech, USA) were probed with 32 P-labeled Praja Clone CH7 [ ] insert antisense strand using ExpressHyb hybridization solution (Clontech, USA) at 68°C. .. A 32 P-labeled β-actin probe supplied with the Northern blots was used as a control to confirm that normalized RNA levels were present in each lane (Clontech).

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  • 94
    TaKaRa human brain total rna
    Expression and functional characterization of R12A-AS1. ( a ) Schematic overview of a miniSINEUP and the target mRNA. 5′ and 3′ UTRs, coding sequence (CDS) of the mRNA and the Binding Domain (BD) and Effector Domain (ED) of the SINEUP are indicated. BD and ED are connected with a spacer sequence, (shown as a black elbow line). ( b ) Genomic organization of the PPP1R12A sense-antisense overlapping region (the elements are not in scale). PPP1R12A coding exons are shown as thick grey bars, UTRs as thin grey bars. R12A-AS1 exons in the two RIKEN full-length <t>cDNA</t> clones are shown in red, and introns by dashed lines. Green vertical lines indicate the position of the AUG codon in the mRNA. The R12A-AS1 isoform, assembled by Cufflinks, is shown by a patterned bar, and the position of the FRAM element is indicated by a red box. ( c ) Expression of PPP1R12A and R12A-AS1 in the FANTOM5 dataset. The tpm values for the top 25 samples of Supplementary Table 3 are presented as a matrix plot. ( d ) Western blot analysis of PPP1R12A in HEK293T cells transfected with 30, 60, 150 and 300 pmol of pR12A-AS1, or empty vector as control, respectively. β-actin is shown as a loading control. Lower panel shows mean intensity of PPP1R12A bands, normalized to actin B. ( e ) Corresponding <t>RNA</t> levels, detected by RT-qP PCR (mean ± s.d. n = 3). *(n = 3, mean + S.D., p
    Human Brain Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human brain total rna/product/TaKaRa
    Average 94 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    human brain total rna - by Bioz Stars, 2020-09
    94/100 stars
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    98
    TaKaRa mouse brain cdna
    Expression of the <t>D-AKAP2</t> protein in various mouse tissues. ( A ) Mouse tissue extracts were prepared as described in Experimental Procedures ). ( B ) Comparison of mouse and human full-length D-AKAP2. Human D-AKAP2 was in vitro translated by using a expression vector containing the <t>cDNA</t> as described in Experimental Procedures . It was then loaded onto SDS-PAGE along with a sample of mouse heart extract and probed with anti-D-AKAP2. M, mouse; H, human. The two arrows indicate the mobilities of the two full-length proteins. Lane 10 and M correspond to the same extract (*).
    Mouse Brain Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse brain cdna/product/TaKaRa
    Average 98 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mouse brain cdna - by Bioz Stars, 2020-09
    98/100 stars
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    99
    TaKaRa human brain mrna rtpcr
    ZNF135 is expressed in fetal and adult human brain. <t>rtPCR</t> products of the expected size for ZNF135 from human adult (lane 2) and fetal (lane 3) whole brain <t>mRNA.</t> Lanes 4 and 5 were duplicate reactions without adding primers. Lane 1 is MW marker.
    Human Brain Mrna Rtpcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human brain mrna rtpcr/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human brain mrna rtpcr - by Bioz Stars, 2020-09
    99/100 stars
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    rna  (TaKaRa)
    94
    TaKaRa rna
    Comparison of Drosophila and mammalian gcm-motif genes. ( A ) Sequence alignment of human GCMa (hGCMa), mouse GCMa (mGCMa), mouse GCMb (mGCMb), and Drosophila GCM (GCM). Amino acid residues shared by these four gene products are highlighted and the gcm-motif is shown below. Similar residues are shown by asterisks. The conserved seven cysteine and four histidine residues are indicated by #. Three absolutely conserved stretches of 9 or 10 amino acids are underlined ( A–C ). Gaps are indicated by dashes. ( B ) Sequence alignment of <t>PCR</t> products. mGCMa, a2, and b are amplified by RT-PCR, using mouse adult head poly(A) + <t>RNA</t> as a template. dGCM2 is a product of PCR using Drosophila genomic DNA as template. Those PCR products have about 70% nucleotide identity to GCM.
    Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1042 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/TaKaRa
    Average 94 stars, based on 1042 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-09
    94/100 stars
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    Image Search Results


    Expression and functional characterization of R12A-AS1. ( a ) Schematic overview of a miniSINEUP and the target mRNA. 5′ and 3′ UTRs, coding sequence (CDS) of the mRNA and the Binding Domain (BD) and Effector Domain (ED) of the SINEUP are indicated. BD and ED are connected with a spacer sequence, (shown as a black elbow line). ( b ) Genomic organization of the PPP1R12A sense-antisense overlapping region (the elements are not in scale). PPP1R12A coding exons are shown as thick grey bars, UTRs as thin grey bars. R12A-AS1 exons in the two RIKEN full-length cDNA clones are shown in red, and introns by dashed lines. Green vertical lines indicate the position of the AUG codon in the mRNA. The R12A-AS1 isoform, assembled by Cufflinks, is shown by a patterned bar, and the position of the FRAM element is indicated by a red box. ( c ) Expression of PPP1R12A and R12A-AS1 in the FANTOM5 dataset. The tpm values for the top 25 samples of Supplementary Table 3 are presented as a matrix plot. ( d ) Western blot analysis of PPP1R12A in HEK293T cells transfected with 30, 60, 150 and 300 pmol of pR12A-AS1, or empty vector as control, respectively. β-actin is shown as a loading control. Lower panel shows mean intensity of PPP1R12A bands, normalized to actin B. ( e ) Corresponding RNA levels, detected by RT-qP PCR (mean ± s.d. n = 3). *(n = 3, mean + S.D., p

    Journal: Scientific Reports

    Article Title: Identification of antisense long noncoding RNAs that function as SINEUPs in human cells

    doi: 10.1038/srep33605

    Figure Lengend Snippet: Expression and functional characterization of R12A-AS1. ( a ) Schematic overview of a miniSINEUP and the target mRNA. 5′ and 3′ UTRs, coding sequence (CDS) of the mRNA and the Binding Domain (BD) and Effector Domain (ED) of the SINEUP are indicated. BD and ED are connected with a spacer sequence, (shown as a black elbow line). ( b ) Genomic organization of the PPP1R12A sense-antisense overlapping region (the elements are not in scale). PPP1R12A coding exons are shown as thick grey bars, UTRs as thin grey bars. R12A-AS1 exons in the two RIKEN full-length cDNA clones are shown in red, and introns by dashed lines. Green vertical lines indicate the position of the AUG codon in the mRNA. The R12A-AS1 isoform, assembled by Cufflinks, is shown by a patterned bar, and the position of the FRAM element is indicated by a red box. ( c ) Expression of PPP1R12A and R12A-AS1 in the FANTOM5 dataset. The tpm values for the top 25 samples of Supplementary Table 3 are presented as a matrix plot. ( d ) Western blot analysis of PPP1R12A in HEK293T cells transfected with 30, 60, 150 and 300 pmol of pR12A-AS1, or empty vector as control, respectively. β-actin is shown as a loading control. Lower panel shows mean intensity of PPP1R12A bands, normalized to actin B. ( e ) Corresponding RNA levels, detected by RT-qP PCR (mean ± s.d. n = 3). *(n = 3, mean + S.D., p

    Article Snippet: AS-ITFG2 cDNA was produced from 1 ug human brain total RNA (Clontech, #636530), using Primescript reverse transcriptase (Clontech, #2680A) and transcript-specific primers.

    Techniques: Expressing, Functional Assay, Sequencing, Binding Assay, Clone Assay, Western Blot, Transfection, Plasmid Preparation, Polymerase Chain Reaction

    ITFG2-AS1 functions as a SINEUP in human cells. ( a ) Genomic organization of the ITFG2/ITFG2-AS1 overlapping region (the elements are not in scale). The ITFG2 exons are shown in red, ITFG2-AS1 in dashed grey, and introns by dashed lines. Green vertical line indicates the position of the AUG codon in the mRNA. The position of the MIRb transposable element is indicated by a red box. ( b ) Expression of ITFG2 and ITFG2-AS1 in the FANTOM5 dataset. The tpm values for the top 25 samples of Supplementary Table 4 are presented as a matrix plot. ( c ) Western blot analysis of ITFG2 in HEK293T cells transfected with increasing amounts of the pITFG2-AS1 construct or empty vector as control, respectively. β−actin is shown as a control. ( d ) Quantification of ITFG2 mRNA and ITFG2-AS1 RNA levels in the HEK293T cell samples from panel c. *(n = 3, mean + S.D., p

    Journal: Scientific Reports

    Article Title: Identification of antisense long noncoding RNAs that function as SINEUPs in human cells

    doi: 10.1038/srep33605

    Figure Lengend Snippet: ITFG2-AS1 functions as a SINEUP in human cells. ( a ) Genomic organization of the ITFG2/ITFG2-AS1 overlapping region (the elements are not in scale). The ITFG2 exons are shown in red, ITFG2-AS1 in dashed grey, and introns by dashed lines. Green vertical line indicates the position of the AUG codon in the mRNA. The position of the MIRb transposable element is indicated by a red box. ( b ) Expression of ITFG2 and ITFG2-AS1 in the FANTOM5 dataset. The tpm values for the top 25 samples of Supplementary Table 4 are presented as a matrix plot. ( c ) Western blot analysis of ITFG2 in HEK293T cells transfected with increasing amounts of the pITFG2-AS1 construct or empty vector as control, respectively. β−actin is shown as a control. ( d ) Quantification of ITFG2 mRNA and ITFG2-AS1 RNA levels in the HEK293T cell samples from panel c. *(n = 3, mean + S.D., p

    Article Snippet: AS-ITFG2 cDNA was produced from 1 ug human brain total RNA (Clontech, #636530), using Primescript reverse transcriptase (Clontech, #2680A) and transcript-specific primers.

    Techniques: Expressing, Western Blot, Transfection, Construct, Plasmid Preparation

    Expression of the D-AKAP2 protein in various mouse tissues. ( A ) Mouse tissue extracts were prepared as described in Experimental Procedures ). ( B ) Comparison of mouse and human full-length D-AKAP2. Human D-AKAP2 was in vitro translated by using a expression vector containing the cDNA as described in Experimental Procedures . It was then loaded onto SDS-PAGE along with a sample of mouse heart extract and probed with anti-D-AKAP2. M, mouse; H, human. The two arrows indicate the mobilities of the two full-length proteins. Lane 10 and M correspond to the same extract (*).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Cloning and mitochondrial localization of full-length D-AKAP2, a protein kinase A anchoring protein

    doi: 10.1073/pnas.051633398

    Figure Lengend Snippet: Expression of the D-AKAP2 protein in various mouse tissues. ( A ) Mouse tissue extracts were prepared as described in Experimental Procedures ). ( B ) Comparison of mouse and human full-length D-AKAP2. Human D-AKAP2 was in vitro translated by using a expression vector containing the cDNA as described in Experimental Procedures . It was then loaded onto SDS-PAGE along with a sample of mouse heart extract and probed with anti-D-AKAP2. M, mouse; H, human. The two arrows indicate the mobilities of the two full-length proteins. Lane 10 and M correspond to the same extract (*).

    Article Snippet: Most of the missing N-terminal region of mouse D-AKAP2 was PCR amplified from mouse brain cDNA (CLONTECH) by using primers based on known mouse and human sequence, and random mouse cDNA sequences homologous to the human gene from GenBank.

    Techniques: Expressing, In Vitro, Plasmid Preparation, SDS Page

    ZNF135 is expressed in fetal and adult human brain. rtPCR products of the expected size for ZNF135 from human adult (lane 2) and fetal (lane 3) whole brain mRNA. Lanes 4 and 5 were duplicate reactions without adding primers. Lane 1 is MW marker.

    Journal: Neuroreport

    Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms

    doi: 10.1097/WNR.0000000000000754

    Figure Lengend Snippet: ZNF135 is expressed in fetal and adult human brain. rtPCR products of the expected size for ZNF135 from human adult (lane 2) and fetal (lane 3) whole brain mRNA. Lanes 4 and 5 were duplicate reactions without adding primers. Lane 1 is MW marker.

    Article Snippet: The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Marker

    Comparison of Drosophila and mammalian gcm-motif genes. ( A ) Sequence alignment of human GCMa (hGCMa), mouse GCMa (mGCMa), mouse GCMb (mGCMb), and Drosophila GCM (GCM). Amino acid residues shared by these four gene products are highlighted and the gcm-motif is shown below. Similar residues are shown by asterisks. The conserved seven cysteine and four histidine residues are indicated by #. Three absolutely conserved stretches of 9 or 10 amino acids are underlined ( A–C ). Gaps are indicated by dashes. ( B ) Sequence alignment of PCR products. mGCMa, a2, and b are amplified by RT-PCR, using mouse adult head poly(A) + RNA as a template. dGCM2 is a product of PCR using Drosophila genomic DNA as template. Those PCR products have about 70% nucleotide identity to GCM.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The gcm-motif: A novel DNA-binding motif conserved inDrosophila and mammals

    doi:

    Figure Lengend Snippet: Comparison of Drosophila and mammalian gcm-motif genes. ( A ) Sequence alignment of human GCMa (hGCMa), mouse GCMa (mGCMa), mouse GCMb (mGCMb), and Drosophila GCM (GCM). Amino acid residues shared by these four gene products are highlighted and the gcm-motif is shown below. Similar residues are shown by asterisks. The conserved seven cysteine and four histidine residues are indicated by #. Three absolutely conserved stretches of 9 or 10 amino acids are underlined ( A–C ). Gaps are indicated by dashes. ( B ) Sequence alignment of PCR products. mGCMa, a2, and b are amplified by RT-PCR, using mouse adult head poly(A) + RNA as a template. dGCM2 is a product of PCR using Drosophila genomic DNA as template. Those PCR products have about 70% nucleotide identity to GCM.

    Article Snippet: For isolation of mouse genes, reverse transcriptase–PCR was performed with mouse adult brain poly(A)+ RNA (CLONTECH CL6616-1).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction