Journal: Cell

Article Title: The Parkinson’s disease protein alpha-synuclein is a modulator of Processing-bodies and mRNA stability

doi: 10.1016/j.cell.2022.05.008

Figure Lengend Snippet: Key resources table

Article Snippet: Human recombinant brain-derived neurotrophic factor (BDNF) , Peprotech , 450-02.

Techniques: Western Blot, Recombinant, Protease Inhibitor, Lysis, Magnetic Beads, Transfection, Expressing, Bicinchoninic Acid Protein Assay, Silver Staining, In Situ, Sample Prep, Luciferase, Reporter Assay, Biomarker Assay, Marker, Generated, Software, Mass Spectrometry, Imaging

Journal: Journal of visualized experiments : JoVE

Article Title: Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

doi: 10.3791/62726

Figure Lengend Snippet: Materials

Article Snippet: Recombinant human-brain-derived neurotrophic factor (BDNF) , Peprotech , 450–02 , Centrifuge briefly before reconstitution. Dissolve 100 μg in 1 mL of PBS to 100 μg/mL, and then add 9 mL of sterile 0.1% BSA-PBS to 10 μg/mL. Aliquot and freeze at −80 °C. Dilute at 1: 1000 for use. (working concentration 10 ng/mL)..

Techniques: Concentration Assay, Transferring, Microscopy, Cell Culture, Knock-Out, Recombinant