human bone marrow derived mscs  (Lonza)


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    Name:
    Human Bone Marrow Stromal Cells
    Description:
    Bone Marrow Stromal Cells 5 million cells
    Catalog Number:
    2M-302
    Price:
    None
    Category:
    Primary and Stem Cells
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    Structured Review

    Lonza human bone marrow derived mscs
    OxPLs Reduce LRP6 Expression on <t>MSC</t> Surface. a , b Representative images of the flow cytometry analysis ( a ) and the percentage of LRP6 + cells ( b ) in human <t>MSCs</t> treated with PBS (Control), 20 μg·mL/ml nLDL or oxLDL (dissolved in PBS) for 30 min. * p
    Bone Marrow Stromal Cells 5 million cells
    https://www.bioz.com/result/human bone marrow derived mscs/product/Lonza
    Average 95 stars, based on 1 article reviews
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    human bone marrow derived mscs - by Bioz Stars, 2021-05
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    1) Product Images from "Oxidized phospholipids are ligands for LRP6"

    Article Title: Oxidized phospholipids are ligands for LRP6

    Journal: Bone Research

    doi: 10.1038/s41413-018-0023-x

    OxPLs Reduce LRP6 Expression on MSC Surface. a , b Representative images of the flow cytometry analysis ( a ) and the percentage of LRP6 + cells ( b ) in human MSCs treated with PBS (Control), 20 μg·mL/ml nLDL or oxLDL (dissolved in PBS) for 30 min. * p
    Figure Legend Snippet: OxPLs Reduce LRP6 Expression on MSC Surface. a , b Representative images of the flow cytometry analysis ( a ) and the percentage of LRP6 + cells ( b ) in human MSCs treated with PBS (Control), 20 μg·mL/ml nLDL or oxLDL (dissolved in PBS) for 30 min. * p

    Techniques Used: Expressing, Flow Cytometry, Cytometry

    Related Articles

    In Vitro:

    Article Title: Radially and Axially Graded Multizonal Bone Graft Substitutes Targeting Critical-Sized Bone Defects from Polycaprolactone/Hydroxyapatite/Tricalcium Phosphate
    Article Snippet: .. Human bone marrow stem cells (Poietics) available from Lonza Walkersville, Inc. were used for in vitro culturing. ..

    Cell Culture:

    Article Title: Mesenchymal stem cells promote metastasis through activation of an ABL-MMP9 signaling axis in lung cancer cells
    Article Snippet: All cell lines were tested routinely for mycoplasma (MycoAlert Plus, Lonza). .. Human bone marrow-derived mesenchymal stem cells (MSC, Lonza) were cultured in DMEM (low glucose, Life Technologies) supplemented with 10% FBS, 2 mM glutamine and antibiotics Pen/Strep. ..

    Article Title: A Mathematical Model Predicting the Coculture Dynamics of Endothelial and Mesenchymal Stem Cells for Tissue Regeneration
    Article Snippet: .. Human bone marrow mesenchymal stem cells and HUVECs (Lonza™, Basel, Switzerland) were cultured using two types of media and their combinations: complete Lonza endothelial growth medium-2 (EGM-2)™, HUVEC-designated medium according to the literature , ; and complete αMEM (minimum essential medium Eagle, alpha modification; Sigma-Aldrich™, St. Louis, MO), MSC-designated medium according to the literature, – supplemented with 10% Gibco® fetal bovine serum (FBS), 1% L-glutamine, 5 ng/mL basic fibroblast growth factor, and 1% antibiotic-antimycotic (Invitrogen™, Carlsbad, CA). ..

    other:

    Article Title: Paracrine-Mediated Neuroprotection and Neuritogenesis of Axotomised Retinal Ganglion Cells by Human Dental Pulp Stem Cells: Comparison with Human Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
    Article Snippet: DPSC/BMSC/AMSC cultures hDPSC were purchased from AllCell LLC (Berkeley, CA) and both hBMSC and hAMSC from Lonza (Slough, UK), and each represented pooled samples from 3 donors.

    Modification:

    Article Title: A Mathematical Model Predicting the Coculture Dynamics of Endothelial and Mesenchymal Stem Cells for Tissue Regeneration
    Article Snippet: .. Human bone marrow mesenchymal stem cells and HUVECs (Lonza™, Basel, Switzerland) were cultured using two types of media and their combinations: complete Lonza endothelial growth medium-2 (EGM-2)™, HUVEC-designated medium according to the literature , ; and complete αMEM (minimum essential medium Eagle, alpha modification; Sigma-Aldrich™, St. Louis, MO), MSC-designated medium according to the literature, – supplemented with 10% Gibco® fetal bovine serum (FBS), 1% L-glutamine, 5 ng/mL basic fibroblast growth factor, and 1% antibiotic-antimycotic (Invitrogen™, Carlsbad, CA). ..

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    Lonza primary human bone marrow derived mscs
    Long-term monitoring of human <t>MSCs</t> in BALB/c mice. a Representative BLI of mice administered with 5 × 10 5 <t>hBM-MSC</t> or hUC-MSC via the IC or IV route. The signal was progressively lost shortly after administration, with no evidence of malignant growth. b Ex vivo bioluminescence imaging of organs within 5 h of administration of the cells. Organs are indicated as the kidneys (k), spleen (s), liver (li), lungs (lu), heart (h) or brain (b). In some occasions, signal foci were seen in the heart of mice that received hUC-MSC IV (red arrow). c BLI images from mice that displayed hUC-MSC signal that persisted beyond day 7 after IV administration (ventral orientation, lower scale). In all cases, the signals had disappeared by day 21 and had not returned by the end of the experiment
    Primary Human Bone Marrow Derived Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    96
    Lonza hbm mscs hbm mscs
    Effects of endocannabinoids on adipogenesis in <t>hBM-MSCs.</t> hBM-MSCs were cultured in 24 well plates. When confluent, the cell viability effects of endocannabinoids were evaluated in hBM-MSCs. NADA (A), AEA (B) and 2-AG (C), were treated for 72 hours in hBM-MSC culture. The cell viability was determined with a detecting reagent, 10 μM of WST-1. Adipogenesis was induced in hBM-MSCs under the presence of the IDX adipocyte differentiation inducing medium. After treating NADA, AEA and 2-AG, in every two or three day, media were exchanged. At the 7 th days in culture, lipid droplets in differentiated adipocytes were stained with Oil Red O (ORO) (D). After dissolving the ORO in isopropyl alcohol, the level of staining was quantified at 500 nm using a spectrometer. Data were normalized by setting the control as 1 (E). Results are the mean ± standard deviation (SD) of three measurements using independent hBM-MSCs from three different donors (n=3). * p ≤0.05 and ** p ≤0.01.
    Hbm Mscs Hbm Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Long-term monitoring of human MSCs in BALB/c mice. a Representative BLI of mice administered with 5 × 10 5 hBM-MSC or hUC-MSC via the IC or IV route. The signal was progressively lost shortly after administration, with no evidence of malignant growth. b Ex vivo bioluminescence imaging of organs within 5 h of administration of the cells. Organs are indicated as the kidneys (k), spleen (s), liver (li), lungs (lu), heart (h) or brain (b). In some occasions, signal foci were seen in the heart of mice that received hUC-MSC IV (red arrow). c BLI images from mice that displayed hUC-MSC signal that persisted beyond day 7 after IV administration (ventral orientation, lower scale). In all cases, the signals had disappeared by day 21 and had not returned by the end of the experiment

    Journal: Stem Cell Research & Therapy

    Article Title: Non-invasive imaging reveals conditions that impact distribution and persistence of cells after in vivo administration

    doi: 10.1186/s13287-018-1076-x

    Figure Lengend Snippet: Long-term monitoring of human MSCs in BALB/c mice. a Representative BLI of mice administered with 5 × 10 5 hBM-MSC or hUC-MSC via the IC or IV route. The signal was progressively lost shortly after administration, with no evidence of malignant growth. b Ex vivo bioluminescence imaging of organs within 5 h of administration of the cells. Organs are indicated as the kidneys (k), spleen (s), liver (li), lungs (lu), heart (h) or brain (b). In some occasions, signal foci were seen in the heart of mice that received hUC-MSC IV (red arrow). c BLI images from mice that displayed hUC-MSC signal that persisted beyond day 7 after IV administration (ventral orientation, lower scale). In all cases, the signals had disappeared by day 21 and had not returned by the end of the experiment

    Article Snippet: Cell preparation Mouse kidney-derived stem cells (mKSCs) [ ], the D1 mouse MSC (mMSC) line (D1 ORL UVA [D1](ATCC® CRL-12424™)), primary human umbilical cord-derived MSCs (hUC-MSCs; collected from consenting donors and produced identically to those already being used in clinical trials by NHS Blood and Transplant (NHSBT)), primary human bone marrow-derived MSCs (hBM-MSCs; Lonza PT-2501), human kidney cells (hKCs; the kidneys deemed unsuitable for transplantation via UK NHSBT [ ]) and RAW264.7 macrophages (European Collection of Authenticated Cell Cultures 91062702) were cultured at 37 °C under a humidified atmosphere with 5% CO2 (culture media are described in Additional file ).

    Techniques: Mouse Assay, Ex Vivo, Imaging

    Cell proliferation and mediator release after in vitro coculture of primary human AML cells and normal mesenchymal stem cells (MSCs), after treatment with the glycolytic inhibitor 2DG. Primary AML cells derived from 14 patients were cultured together with normal MSCs in transwell cocultures for 3 days in the presence of 2DG, before proliferation was assayed using the [ 3 H]-thymidine incorporation assay. Mediator levels were also determined in coculture supernatants with and without 48-hour treatment with 2DG, using multiplex analysis. ( A ) Effect of 2DG on cell proliferation in MSC-AML cocultures. Proliferation of MSCs or AML cells in 2DG-treated cocultures relative to the inhibitor-free control cocultures (shown as stippled line at 100%) was calculated and an antiproliferative effect by 2DG was then observed both for the MSCs (derived from one donor) and the AML cells (derived from 14 patients) during coculture. The median proliferation of the MSC cells and the AML primary cells after treatment in cocultures is indicated in the figure ( ‒ ). ( B ) The constitutive release of soluble mediators in cocultures after 48-hour treatment with 2DG. The results are presented as the median levels with 25/75 percentiles (boxes), 5/95 percentiles (whiskers). The mediator concentrations (pg/mL) are given on the X-axis as log-values. Significant p -values are shown to the right (Wilcoxon signed rank test; 2DG-treated cocultures compared to untreated cocultures). Grey boxes illustrate mediator levels in untreated cocultures and white boxes indicate levels after treatment with 0.6 mM 2DG.

    Journal: Cells

    Article Title: Targeting Cellular Metabolism in Acute Myeloid Leukemia and the Role of Patient Heterogeneity

    doi: 10.3390/cells9051155

    Figure Lengend Snippet: Cell proliferation and mediator release after in vitro coculture of primary human AML cells and normal mesenchymal stem cells (MSCs), after treatment with the glycolytic inhibitor 2DG. Primary AML cells derived from 14 patients were cultured together with normal MSCs in transwell cocultures for 3 days in the presence of 2DG, before proliferation was assayed using the [ 3 H]-thymidine incorporation assay. Mediator levels were also determined in coculture supernatants with and without 48-hour treatment with 2DG, using multiplex analysis. ( A ) Effect of 2DG on cell proliferation in MSC-AML cocultures. Proliferation of MSCs or AML cells in 2DG-treated cocultures relative to the inhibitor-free control cocultures (shown as stippled line at 100%) was calculated and an antiproliferative effect by 2DG was then observed both for the MSCs (derived from one donor) and the AML cells (derived from 14 patients) during coculture. The median proliferation of the MSC cells and the AML primary cells after treatment in cocultures is indicated in the figure ( ‒ ). ( B ) The constitutive release of soluble mediators in cocultures after 48-hour treatment with 2DG. The results are presented as the median levels with 25/75 percentiles (boxes), 5/95 percentiles (whiskers). The mediator concentrations (pg/mL) are given on the X-axis as log-values. Significant p -values are shown to the right (Wilcoxon signed rank test; 2DG-treated cocultures compared to untreated cocultures). Grey boxes illustrate mediator levels in untreated cocultures and white boxes indicate levels after treatment with 0.6 mM 2DG.

    Article Snippet: Cocultures of Human Mesenchymal Stem Cells (MSCs) and AML CellsHuman MSCs (Lonza, Cambrex BioScience, Walkersville, MD, USA) derived from a healthy donor (MSC24539) were expanded in complete MSC growth medium (MSCGM™; Lonza) with 10% heat-inactivated FBS and 4 mM L-glutamine.

    Techniques: In Vitro, Derivative Assay, Cell Culture, Thymidine Incorporation Assay, Multiplex Assay

    Expression of CD73, CD90, CD105 in LNC and BMMSC. P4 LNC and P4 BMMSC cultured on 2D Matrigel in MESCM were subjected to fluorescence-activated cell sorting (FACS) analysis of MSC markers ( A – F , n = 3).

    Journal: Scientific Reports

    Article Title: Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model

    doi: 10.1038/s41598-018-24862-6

    Figure Lengend Snippet: Expression of CD73, CD90, CD105 in LNC and BMMSC. P4 LNC and P4 BMMSC cultured on 2D Matrigel in MESCM were subjected to fluorescence-activated cell sorting (FACS) analysis of MSC markers ( A – F , n = 3).

    Article Snippet: The second passage of bone marrow-derived MSC (BMMSC, PT-2501) was obtained from LONZA (Allendale, NJ) and cultured in parallel.

    Techniques: Expressing, Cell Culture, Fluorescence, FACS

    LNC express more ESC, MSC and NC markers. P4 LNC and P4 BMMSC cultured on 2D Matrigel in MESCM were subjected to qRT-PCR for transcription expression of ESC markers ( A ), MSC and neural crest markers ( B , n = 3, *p

    Journal: Scientific Reports

    Article Title: Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model

    doi: 10.1038/s41598-018-24862-6

    Figure Lengend Snippet: LNC express more ESC, MSC and NC markers. P4 LNC and P4 BMMSC cultured on 2D Matrigel in MESCM were subjected to qRT-PCR for transcription expression of ESC markers ( A ), MSC and neural crest markers ( B , n = 3, *p

    Article Snippet: The second passage of bone marrow-derived MSC (BMMSC, PT-2501) was obtained from LONZA (Allendale, NJ) and cultured in parallel.

    Techniques: Cell Culture, Quantitative RT-PCR, Expressing

    Effects of endocannabinoids on adipogenesis in hBM-MSCs. hBM-MSCs were cultured in 24 well plates. When confluent, the cell viability effects of endocannabinoids were evaluated in hBM-MSCs. NADA (A), AEA (B) and 2-AG (C), were treated for 72 hours in hBM-MSC culture. The cell viability was determined with a detecting reagent, 10 μM of WST-1. Adipogenesis was induced in hBM-MSCs under the presence of the IDX adipocyte differentiation inducing medium. After treating NADA, AEA and 2-AG, in every two or three day, media were exchanged. At the 7 th days in culture, lipid droplets in differentiated adipocytes were stained with Oil Red O (ORO) (D). After dissolving the ORO in isopropyl alcohol, the level of staining was quantified at 500 nm using a spectrometer. Data were normalized by setting the control as 1 (E). Results are the mean ± standard deviation (SD) of three measurements using independent hBM-MSCs from three different donors (n=3). * p ≤0.05 and ** p ≤0.01.

    Journal: Biomolecules & Therapeutics

    Article Title: A Cannabinoid Receptor Agonist N-Arachidonoyl Dopamine Inhibits Adipocyte Differentiation in Human Mesenchymal Stem Cells

    doi: 10.4062/biomolther.2014.137

    Figure Lengend Snippet: Effects of endocannabinoids on adipogenesis in hBM-MSCs. hBM-MSCs were cultured in 24 well plates. When confluent, the cell viability effects of endocannabinoids were evaluated in hBM-MSCs. NADA (A), AEA (B) and 2-AG (C), were treated for 72 hours in hBM-MSC culture. The cell viability was determined with a detecting reagent, 10 μM of WST-1. Adipogenesis was induced in hBM-MSCs under the presence of the IDX adipocyte differentiation inducing medium. After treating NADA, AEA and 2-AG, in every two or three day, media were exchanged. At the 7 th days in culture, lipid droplets in differentiated adipocytes were stained with Oil Red O (ORO) (D). After dissolving the ORO in isopropyl alcohol, the level of staining was quantified at 500 nm using a spectrometer. Data were normalized by setting the control as 1 (E). Results are the mean ± standard deviation (SD) of three measurements using independent hBM-MSCs from three different donors (n=3). * p ≤0.05 and ** p ≤0.01.

    Article Snippet: Cell culture and differentiation of hBM-MSCs hBM-MSCs were purchased from Lonza, Inc. (Walkersville, MD, USA). hBM-MSCs were cultured according to the manufacturer’s instructions with minor modifications. hBM-MSCs were maintained in Dulbecco’s modified eagle’s medium (DMEM) with low glucose (1 g/L glucose) containing 10% fetal bovine serum (FBS) (Lonza), supplemented with antibiotics and GlutamaxTM (Invitrogen, Carlsbad, CA, USA).

    Techniques: Cell Culture, Staining, Standard Deviation

    Transcriptional expression profile of CNR1, CNR2 and TRPV1 during adipogenesis in hBM-MSCs. Adipocyte differentiation was induced in hBM-MSCs by exchanging culture media supplemented with 1 μg/ml insulin, 0.1 μM dexamethasone and 0.5 mM isobutylmethylxanthine (IDX). At the seventh day after the induction of adipogenesis, total RNA was extracted and Q-RT-PCR analysis was performed for (A) CNR1 and (B) TRPV1. Values represent the mean expression ± SE of the mRNA of the various genes relative to human GAPDH expression (n=3), * p ≤0.05, ** p ≤0.01.

    Journal: Biomolecules & Therapeutics

    Article Title: A Cannabinoid Receptor Agonist N-Arachidonoyl Dopamine Inhibits Adipocyte Differentiation in Human Mesenchymal Stem Cells

    doi: 10.4062/biomolther.2014.137

    Figure Lengend Snippet: Transcriptional expression profile of CNR1, CNR2 and TRPV1 during adipogenesis in hBM-MSCs. Adipocyte differentiation was induced in hBM-MSCs by exchanging culture media supplemented with 1 μg/ml insulin, 0.1 μM dexamethasone and 0.5 mM isobutylmethylxanthine (IDX). At the seventh day after the induction of adipogenesis, total RNA was extracted and Q-RT-PCR analysis was performed for (A) CNR1 and (B) TRPV1. Values represent the mean expression ± SE of the mRNA of the various genes relative to human GAPDH expression (n=3), * p ≤0.05, ** p ≤0.01.

    Article Snippet: Cell culture and differentiation of hBM-MSCs hBM-MSCs were purchased from Lonza, Inc. (Walkersville, MD, USA). hBM-MSCs were cultured according to the manufacturer’s instructions with minor modifications. hBM-MSCs were maintained in Dulbecco’s modified eagle’s medium (DMEM) with low glucose (1 g/L glucose) containing 10% fetal bovine serum (FBS) (Lonza), supplemented with antibiotics and GlutamaxTM (Invitrogen, Carlsbad, CA, USA).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    The effects of rimonabant on adipogenesis in hBM-MSCs and PPARγ transactivation. (A) The concentration-dependent effect of rimonabant on adipogenesis in hBM-MSCs was evaluated in 24 well plates. The effect of rimonabant was compared with those of aspirin, glibenclamide and troglitazone. Cell culture supernatants were harvested for the measurement of adiponectin by ELISA at the 7 th days after exchanging media containing with IDX. (B) CV-1 cells were transiently cotransfected with the PPARγ expression vector and the PPRE-TK-Luciferase reporter, and then treated with vehicle, rimonabant (RIMO), AEA, NADA and 2-AG (1, 3, and 10 μM) or troglitazone (TRO, 1 μM). Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Journal: Biomolecules & Therapeutics

    Article Title: A Cannabinoid Receptor Agonist N-Arachidonoyl Dopamine Inhibits Adipocyte Differentiation in Human Mesenchymal Stem Cells

    doi: 10.4062/biomolther.2014.137

    Figure Lengend Snippet: The effects of rimonabant on adipogenesis in hBM-MSCs and PPARγ transactivation. (A) The concentration-dependent effect of rimonabant on adipogenesis in hBM-MSCs was evaluated in 24 well plates. The effect of rimonabant was compared with those of aspirin, glibenclamide and troglitazone. Cell culture supernatants were harvested for the measurement of adiponectin by ELISA at the 7 th days after exchanging media containing with IDX. (B) CV-1 cells were transiently cotransfected with the PPARγ expression vector and the PPRE-TK-Luciferase reporter, and then treated with vehicle, rimonabant (RIMO), AEA, NADA and 2-AG (1, 3, and 10 μM) or troglitazone (TRO, 1 μM). Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Article Snippet: Cell culture and differentiation of hBM-MSCs hBM-MSCs were purchased from Lonza, Inc. (Walkersville, MD, USA). hBM-MSCs were cultured according to the manufacturer’s instructions with minor modifications. hBM-MSCs were maintained in Dulbecco’s modified eagle’s medium (DMEM) with low glucose (1 g/L glucose) containing 10% fetal bovine serum (FBS) (Lonza), supplemented with antibiotics and GlutamaxTM (Invitrogen, Carlsbad, CA, USA).

    Techniques: Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing, Plasmid Preparation, Luciferase, Standard Deviation

    Effects of endocannabinoids on adipocyte differentiation marker expression during adipogenesis in hBM-MSCs. When hBM-MSCs were in confluent state, adipogenesis was induced by exchanging media with the IDX adipogenic cocktail. At the 7 th days in culture, total RNA samples were extracted and Q-RT-PCR was performed for PPARγ (A), FABP4 (B) and adiponectin (C). GAPDH was used as an internal control for Q-RT-PCR standardization. Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Journal: Biomolecules & Therapeutics

    Article Title: A Cannabinoid Receptor Agonist N-Arachidonoyl Dopamine Inhibits Adipocyte Differentiation in Human Mesenchymal Stem Cells

    doi: 10.4062/biomolther.2014.137

    Figure Lengend Snippet: Effects of endocannabinoids on adipocyte differentiation marker expression during adipogenesis in hBM-MSCs. When hBM-MSCs were in confluent state, adipogenesis was induced by exchanging media with the IDX adipogenic cocktail. At the 7 th days in culture, total RNA samples were extracted and Q-RT-PCR was performed for PPARγ (A), FABP4 (B) and adiponectin (C). GAPDH was used as an internal control for Q-RT-PCR standardization. Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Article Snippet: Cell culture and differentiation of hBM-MSCs hBM-MSCs were purchased from Lonza, Inc. (Walkersville, MD, USA). hBM-MSCs were cultured according to the manufacturer’s instructions with minor modifications. hBM-MSCs were maintained in Dulbecco’s modified eagle’s medium (DMEM) with low glucose (1 g/L glucose) containing 10% fetal bovine serum (FBS) (Lonza), supplemented with antibiotics and GlutamaxTM (Invitrogen, Carlsbad, CA, USA).

    Techniques: Marker, Expressing, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Effects of endocannabinoids on adiponectin production during adipogenesis in hBM-MSCs. When hBM-MSCs were in confluent state, adipogenesis was induced by exchanging media with the IDX adipogenic cocktail. ELISA was performed to measure the concentration of adiponectin (A) accumulated in cell culture supernatants for 48 hours after the last medium exchange. The concentration-dependent effect of arachidonyl dopamine (NADA) on the inhibition of adipogenesis was evaluated (B). Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Journal: Biomolecules & Therapeutics

    Article Title: A Cannabinoid Receptor Agonist N-Arachidonoyl Dopamine Inhibits Adipocyte Differentiation in Human Mesenchymal Stem Cells

    doi: 10.4062/biomolther.2014.137

    Figure Lengend Snippet: Effects of endocannabinoids on adiponectin production during adipogenesis in hBM-MSCs. When hBM-MSCs were in confluent state, adipogenesis was induced by exchanging media with the IDX adipogenic cocktail. ELISA was performed to measure the concentration of adiponectin (A) accumulated in cell culture supernatants for 48 hours after the last medium exchange. The concentration-dependent effect of arachidonyl dopamine (NADA) on the inhibition of adipogenesis was evaluated (B). Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Article Snippet: Cell culture and differentiation of hBM-MSCs hBM-MSCs were purchased from Lonza, Inc. (Walkersville, MD, USA). hBM-MSCs were cultured according to the manufacturer’s instructions with minor modifications. hBM-MSCs were maintained in Dulbecco’s modified eagle’s medium (DMEM) with low glucose (1 g/L glucose) containing 10% fetal bovine serum (FBS) (Lonza), supplemented with antibiotics and GlutamaxTM (Invitrogen, Carlsbad, CA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Cell Culture, Inhibition, Expressing, Standard Deviation

    Effects of rimonabant and capsazepine on the NADA-dependent inhibition of adipogenesis in hBM-MSCs. hBM-MSCs were cultured in 24 well plates. When confluent, the cell viability effects were evaluated for rimonabant (A) and capsazepine (B), both of which were treated for 72 hours in hBM-MSC culture. The cell viability was determined by WST-1 assay. For testing the effects of capsazepine (C) and rimonabant (D) on the NADA-induced inhibition on the adipogenesis in hBM-MSCs, cell culture supernatants were harvested for the measurement of adiponectin by ELISA at the 7 th days after exchanging media containing with IDX. Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Journal: Biomolecules & Therapeutics

    Article Title: A Cannabinoid Receptor Agonist N-Arachidonoyl Dopamine Inhibits Adipocyte Differentiation in Human Mesenchymal Stem Cells

    doi: 10.4062/biomolther.2014.137

    Figure Lengend Snippet: Effects of rimonabant and capsazepine on the NADA-dependent inhibition of adipogenesis in hBM-MSCs. hBM-MSCs were cultured in 24 well plates. When confluent, the cell viability effects were evaluated for rimonabant (A) and capsazepine (B), both of which were treated for 72 hours in hBM-MSC culture. The cell viability was determined by WST-1 assay. For testing the effects of capsazepine (C) and rimonabant (D) on the NADA-induced inhibition on the adipogenesis in hBM-MSCs, cell culture supernatants were harvested for the measurement of adiponectin by ELISA at the 7 th days after exchanging media containing with IDX. Values represent the mean expression ± standard deviation (SD) (n=3). * p ≤0.05 and ** p ≤0.01.

    Article Snippet: Cell culture and differentiation of hBM-MSCs hBM-MSCs were purchased from Lonza, Inc. (Walkersville, MD, USA). hBM-MSCs were cultured according to the manufacturer’s instructions with minor modifications. hBM-MSCs were maintained in Dulbecco’s modified eagle’s medium (DMEM) with low glucose (1 g/L glucose) containing 10% fetal bovine serum (FBS) (Lonza), supplemented with antibiotics and GlutamaxTM (Invitrogen, Carlsbad, CA, USA).

    Techniques: Inhibition, Cell Culture, WST-1 Assay, Enzyme-linked Immunosorbent Assay, Expressing, Standard Deviation