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TaKaRa semi quantitative rt pcr
Semi Quantitative Rt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 27538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/semi quantitative rt pcr/product/TaKaRa
Average 94 stars, based on 27538 article reviews
Price from $9.99 to $1999.99
semi quantitative rt pcr - by Bioz Stars, 2020-09
94/100 stars

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Electroporation:

Article Title: The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice
Article Snippet: .. Plasmids and in u tero electroporation Fetal human brain RNA (Clontech) was reverse transcribed with QuantScript RT kit using oligo (dT)15 primers (Tiangen). .. TBC1D3 cDNA was acquired by PCR and cloned into pE/nestin-EGFP vectors (a kind gift from Dr. H. Okano) , to generate the pE/nestin-TBC1D3 plasmid after replacing the EGFP sequence.

Amplification:

Article Title: ADAM 23/MDC3, a Human Disintegrin That Promotes Cell Adhesion via Interaction with the ?v?3 Integrin through an RGD-independent Mechanism
Article Snippet: .. To obtain this DNA fragment, we performed PCR amplification of a human brain cDNA ( Clontech ) with two specific primers, 5′-CAACAAAGCTATTTGAGCCCACGG and 5′-TTGGTGGGCACTGACCAGAGTCT, derived from the sequence. .. The PCR reaction was carried out in a GeneAmp 2400 PCR system from Perkin Elmer (Norwalk, CT) for 40 cycles of denaturation (94°C, 15 s), annealing (64°C, 20 s), and extension (72°C, 20 s).

Plasmid Preparation:

Article Title: ?-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform
Article Snippet: .. The pAS2–1/NHE5 (amino acids 544–896) plasmid was cotransformed with a human brain cDNA library subcloned into the pACT2 vector in-frame with the GAL4 activation domain (Clontech) into the Saccharomyces cerevisiae strain AH109 by using a modified lithium acetate method ( ). .. More than 2 × 106 clones were screened on the synthetic complete media containing 0.67% bacto-yeast nitrogen base without amino acids, 2% glucose, 1.5% bacto-agar, and 0.2% Leu-Trp-His-Ade drop-out mix.

Polymerase Chain Reaction:

Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

Article Title: ADAM 23/MDC3, a Human Disintegrin That Promotes Cell Adhesion via Interaction with the ?v?3 Integrin through an RGD-independent Mechanism
Article Snippet: .. To obtain this DNA fragment, we performed PCR amplification of a human brain cDNA ( Clontech ) with two specific primers, 5′-CAACAAAGCTATTTGAGCCCACGG and 5′-TTGGTGGGCACTGACCAGAGTCT, derived from the sequence. .. The PCR reaction was carried out in a GeneAmp 2400 PCR system from Perkin Elmer (Norwalk, CT) for 40 cycles of denaturation (94°C, 15 s), annealing (64°C, 20 s), and extension (72°C, 20 s).

Article Title: Pathogenic exon-trapping by SVA retrotransposon and rescue in Fukuyama muscular dystrophy
Article Snippet: .. To detect abnormally-spliced RT-PCR products from FCMD, ARH, and NLSDM patients, and from human brain AB627340 cDNA, long range PCR was performed using LA Taq with LA Taq Buffer II (Takara), adding dimethyl sulfoxide and 7-deaza-dGTP (Roche). .. The RT-PCR products were directly sequenced (FCMD and NLSDM), or cloned with the TOPO TA Cloning Kit (Invitrogen) before sequencing (ARH and AB627340).

Northern blot:

Article Title: A Human Mitochondrial GTP Binding Protein Related to tRNA Modification May Modulate Phenotypic Expression of the Deafness-Associated Mitochondrial 12S rRNA Mutation
Article Snippet: .. A 12-lane human multiple tissue RNA blot and an 8-lane human brain tissue RNA blot (Clontech) containing 2 μg of poly(A)+ RNA/lane were used for the present study. .. A 636-bp GTPBP3 cDNA fragment corresponding to nt 1220 to 1855 was random prime labeled with [32 P]dATP and hybridized with the RNA blots according to the manufacturer's instructions.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

Article Title: Pathogenic exon-trapping by SVA retrotransposon and rescue in Fukuyama muscular dystrophy
Article Snippet: .. To detect abnormally-spliced RT-PCR products from FCMD, ARH, and NLSDM patients, and from human brain AB627340 cDNA, long range PCR was performed using LA Taq with LA Taq Buffer II (Takara), adding dimethyl sulfoxide and 7-deaza-dGTP (Roche). .. The RT-PCR products were directly sequenced (FCMD and NLSDM), or cloned with the TOPO TA Cloning Kit (Invitrogen) before sequencing (ARH and AB627340).

Sequencing:

Article Title: ADAM 23/MDC3, a Human Disintegrin That Promotes Cell Adhesion via Interaction with the ?v?3 Integrin through an RGD-independent Mechanism
Article Snippet: .. To obtain this DNA fragment, we performed PCR amplification of a human brain cDNA ( Clontech ) with two specific primers, 5′-CAACAAAGCTATTTGAGCCCACGG and 5′-TTGGTGGGCACTGACCAGAGTCT, derived from the sequence. .. The PCR reaction was carried out in a GeneAmp 2400 PCR system from Perkin Elmer (Norwalk, CT) for 40 cycles of denaturation (94°C, 15 s), annealing (64°C, 20 s), and extension (72°C, 20 s).

Activation Assay:

Article Title: ?-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform
Article Snippet: .. The pAS2–1/NHE5 (amino acids 544–896) plasmid was cotransformed with a human brain cDNA library subcloned into the pACT2 vector in-frame with the GAL4 activation domain (Clontech) into the Saccharomyces cerevisiae strain AH109 by using a modified lithium acetate method ( ). .. More than 2 × 106 clones were screened on the synthetic complete media containing 0.67% bacto-yeast nitrogen base without amino acids, 2% glucose, 1.5% bacto-agar, and 0.2% Leu-Trp-His-Ade drop-out mix.

cDNA Library Assay:

Article Title: ?-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform
Article Snippet: .. The pAS2–1/NHE5 (amino acids 544–896) plasmid was cotransformed with a human brain cDNA library subcloned into the pACT2 vector in-frame with the GAL4 activation domain (Clontech) into the Saccharomyces cerevisiae strain AH109 by using a modified lithium acetate method ( ). .. More than 2 × 106 clones were screened on the synthetic complete media containing 0.67% bacto-yeast nitrogen base without amino acids, 2% glucose, 1.5% bacto-agar, and 0.2% Leu-Trp-His-Ade drop-out mix.

DNA Sequencing:

Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

Expressing:

Article Title: Assessment of mutations in KCNN2 andZNF135 to patient neurological symptoms
Article Snippet: .. The rtPCR on human brain mRNA rtPCR on human brain mRNA used Poly A+ RNA from adult and fetal brains purchased from Clontech Laboratories, Inc. (Mountain View, CA) Expression of hZNF135 mRNA was tested using gene specific primers and AccessQuick RT-PCR system (Promega Corp. Madison, WI) followed by DNA sequencing of the PCR product. .. Transfections into HEK293 cells used Lipofectamine (Thermo Fisher Scientific, Inc., Waltham, MA) on poly-D-lysine-coated microscopic cover glasses in 12-well tissue culture plates.

Modification:

Article Title: ?-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform
Article Snippet: .. The pAS2–1/NHE5 (amino acids 544–896) plasmid was cotransformed with a human brain cDNA library subcloned into the pACT2 vector in-frame with the GAL4 activation domain (Clontech) into the Saccharomyces cerevisiae strain AH109 by using a modified lithium acetate method ( ). .. More than 2 × 106 clones were screened on the synthetic complete media containing 0.67% bacto-yeast nitrogen base without amino acids, 2% glucose, 1.5% bacto-agar, and 0.2% Leu-Trp-His-Ade drop-out mix.

Derivative Assay:

Article Title: ADAM 23/MDC3, a Human Disintegrin That Promotes Cell Adhesion via Interaction with the ?v?3 Integrin through an RGD-independent Mechanism
Article Snippet: .. To obtain this DNA fragment, we performed PCR amplification of a human brain cDNA ( Clontech ) with two specific primers, 5′-CAACAAAGCTATTTGAGCCCACGG and 5′-TTGGTGGGCACTGACCAGAGTCT, derived from the sequence. .. The PCR reaction was carried out in a GeneAmp 2400 PCR system from Perkin Elmer (Norwalk, CT) for 40 cycles of denaturation (94°C, 15 s), annealing (64°C, 20 s), and extension (72°C, 20 s).

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