Structured Review

Michrom htc pal autosampler
Htc Pal Autosampler, supplied by Michrom, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/htc pal autosampler/product/Michrom
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
htc pal autosampler - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

High Performance Liquid Chromatography:

Article Title: Kinase-interacting substrate screening is a novel method to identify kinase substrates
Article Snippet: .. Nanoelectrospray tandem mass analysis was performed using a Finnigan LTQ/Orbitrap mass spectrometry (for the analysis of Rho-kinase, PKA, MAPK1, CaMK1, CDK5, PAK7, and PKN) or Q Exactive mass spectrometry (for the analysis of LYN and FYN; Thermo Fisher Scientific) system combined with an HTC-PAL autosampler and a Paradigm MS4 HPLC System (Michrom BioResources Inc.) using a C18 reversed-phase column and an ADVANCE Plug-and-Play NanoSpray Source (Michrom BioResources Inc.). .. A peak list was generated and calibrated using MaxQuant software (version1.3.0.5; ).

Article Title: Discovery of long-range inhibitory signaling to ensure single axon formation
Article Snippet: .. Nanoelectrospray tandem mass analysis was performed using a Q Exactive mass spectrometry system (Thermo Fisher Scientific Inc.) combined with an HTC-PAL autosampler and a Paradigm MS4 HPLC System (Michrom BioResources Inc.) using a C18 reverse-phase column and a Michrom ADVANCE Plug-and-Play NanoSpray Source. .. A peak list was generated and calibrated using MaxQuant software.

Article Title: A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance
Article Snippet: .. Briefly, we used an LTQ Orbitrap XL mass spectrometer (Thermo Scientific) connected to an HTC-PAL autosampler and a Paradigm MS4 HPLC with a C18 reversed-phase column and ADVANCE Plug and Play Nano Source (Michrom Bioresources). .. The reversed-phase chromatography condition was a linear gradient (0 min, 5% B; 70 min, 100% B) of solvent A (2% CH3 CN with 0.1% trifluoroacetic acid (TFA)) and solvent B (98% CH3 CN with 0.1% TFA) at a flow rate of 500 nl min−1 .

Article Title: Bruton's Tyrosine Kinase (BTK) and Vav1 Contribute to Dectin1-Dependent Phagocytosis of Candida albicans in Macrophages
Article Snippet: .. LC-ESI-MS analysis was performed using a Micromass LCT mass spectrometer (Micromass MS Technologies, USA) and a Paradigm MG4 HPLC system equipped with a HTC PAL autosampler (Michrom BioResources, USA) and a Waters Symmetry 5 µm C8 column (2.1×50 mm, MeCN∶H2 O (0.1% formic acid) gradient mobile phase, 150 µL/min). .. HPLC purifications were achieved using an Agilent 1100 Series HPLC system equipped with a Waters Delta Pak 15 µm, 100 Å C18 column (7.8×300 mm) using A: H2 O, B: MeCN and C: 1% aqueous trifluoroacetic acid as mobile phase (3 mL/min). (R/S)-1-Boc-3-Hydroxypiperidine (1.05 g, 5 mmol) was dissolved in CH2 Cl2 (25 mL) and subsequently triethylamine (1.39 mL, 10 mmol) and methanesulfonyl chloride (0.394 mL, 5.1 mmol) were added.

Article Title: Substrate Filtering by the Active Site Crossover Loop in UCHL3 Revealed by Sortagging and Gain-of-function Mutations
Article Snippet: .. Mass Spectrometry —Liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) was performed on a Micromass LCT mass spectrometer (Micromass MS Technologies) and a Paradigm MG4 HPLC system equipped with a HTC PAL autosampler (Michrom BioResources) and a Waters Symmetry 5 μ m C8 column (2.1 × 50 mm, MeCN:H2 O (0.1% formic acid) gradient mobile phase, 150 μl/min). .. Cloning and Protein Expression —Sortase A was expressed and purified as described ( ).

Article Title: A Straight Path to Circular Proteins *
Article Snippet: .. ESI-MS was performed on a Micromass LCT mass spectrometer (Micromass® MS Technologies) and a Paradigm MG4 HPLC system equipped with an HTC PAL autosampler (Michrom BioResources) and a Waters symmetry 5-μm C8 column (2.1 × 50 mm, MeCN:H2 O (0.1% formic acid) gradient mobile phase, 150 μl/min). .. G5 -eGFP-LPETG-His6 (50 μ m ) was circularized by treatment with sortase A (50 μ m ) in sortase reaction buffer (50 m m Tris, pH 7.5, 150 m m NaCl, 10 m m CaCl2 ) for 24 h at 37 °C.

Article Title: Identification of host cell factors required for intoxication through use of modified cholera toxin
Article Snippet: .. We were unable to observe the Alexa Fluor 647 peptide with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer, so we inferred the molecular weight and activity as a nucleophile by setting up a test transpeptidation reaction with sortase A and an LPETG-tagged GFP substrate as described previously , then observing the mass change in the transpeptidation product by electrospray ionization mass spectrometry on a liquid chromatography mass spectrometer (Micromass MS Technologies), an HPLC system equipped with an HTC PAL autosampler (Paradigm MG4; Michrom BioResources), and a Symmetry 5 mm C8 column (2.1 × 50 mm, MeCN/H2O (0.1% formic acid) gradient mobile phase, 150 ml/min; Waters). .. The predicted molecular weight for the Alexa Fluor 647 peptide is 1,155.06 g/mol, obs = 1155.0 g/mol.

Mass Spectrometry:

Article Title: Kinase-interacting substrate screening is a novel method to identify kinase substrates
Article Snippet: .. Nanoelectrospray tandem mass analysis was performed using a Finnigan LTQ/Orbitrap mass spectrometry (for the analysis of Rho-kinase, PKA, MAPK1, CaMK1, CDK5, PAK7, and PKN) or Q Exactive mass spectrometry (for the analysis of LYN and FYN; Thermo Fisher Scientific) system combined with an HTC-PAL autosampler and a Paradigm MS4 HPLC System (Michrom BioResources Inc.) using a C18 reversed-phase column and an ADVANCE Plug-and-Play NanoSpray Source (Michrom BioResources Inc.). .. A peak list was generated and calibrated using MaxQuant software (version1.3.0.5; ).

Article Title: Discovery of long-range inhibitory signaling to ensure single axon formation
Article Snippet: .. Nanoelectrospray tandem mass analysis was performed using a Q Exactive mass spectrometry system (Thermo Fisher Scientific Inc.) combined with an HTC-PAL autosampler and a Paradigm MS4 HPLC System (Michrom BioResources Inc.) using a C18 reverse-phase column and a Michrom ADVANCE Plug-and-Play NanoSpray Source. .. A peak list was generated and calibrated using MaxQuant software.

Article Title: A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance
Article Snippet: .. Briefly, we used an LTQ Orbitrap XL mass spectrometer (Thermo Scientific) connected to an HTC-PAL autosampler and a Paradigm MS4 HPLC with a C18 reversed-phase column and ADVANCE Plug and Play Nano Source (Michrom Bioresources). .. The reversed-phase chromatography condition was a linear gradient (0 min, 5% B; 70 min, 100% B) of solvent A (2% CH3 CN with 0.1% trifluoroacetic acid (TFA)) and solvent B (98% CH3 CN with 0.1% TFA) at a flow rate of 500 nl min−1 .

Article Title: Bruton's Tyrosine Kinase (BTK) and Vav1 Contribute to Dectin1-Dependent Phagocytosis of Candida albicans in Macrophages
Article Snippet: .. LC-ESI-MS analysis was performed using a Micromass LCT mass spectrometer (Micromass MS Technologies, USA) and a Paradigm MG4 HPLC system equipped with a HTC PAL autosampler (Michrom BioResources, USA) and a Waters Symmetry 5 µm C8 column (2.1×50 mm, MeCN∶H2 O (0.1% formic acid) gradient mobile phase, 150 µL/min). .. HPLC purifications were achieved using an Agilent 1100 Series HPLC system equipped with a Waters Delta Pak 15 µm, 100 Å C18 column (7.8×300 mm) using A: H2 O, B: MeCN and C: 1% aqueous trifluoroacetic acid as mobile phase (3 mL/min). (R/S)-1-Boc-3-Hydroxypiperidine (1.05 g, 5 mmol) was dissolved in CH2 Cl2 (25 mL) and subsequently triethylamine (1.39 mL, 10 mmol) and methanesulfonyl chloride (0.394 mL, 5.1 mmol) were added.

Article Title: Substrate Filtering by the Active Site Crossover Loop in UCHL3 Revealed by Sortagging and Gain-of-function Mutations
Article Snippet: .. Mass Spectrometry —Liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) was performed on a Micromass LCT mass spectrometer (Micromass MS Technologies) and a Paradigm MG4 HPLC system equipped with a HTC PAL autosampler (Michrom BioResources) and a Waters Symmetry 5 μ m C8 column (2.1 × 50 mm, MeCN:H2 O (0.1% formic acid) gradient mobile phase, 150 μl/min). .. Cloning and Protein Expression —Sortase A was expressed and purified as described ( ).

Article Title: A Straight Path to Circular Proteins *
Article Snippet: .. ESI-MS was performed on a Micromass LCT mass spectrometer (Micromass® MS Technologies) and a Paradigm MG4 HPLC system equipped with an HTC PAL autosampler (Michrom BioResources) and a Waters symmetry 5-μm C8 column (2.1 × 50 mm, MeCN:H2 O (0.1% formic acid) gradient mobile phase, 150 μl/min). .. G5 -eGFP-LPETG-His6 (50 μ m ) was circularized by treatment with sortase A (50 μ m ) in sortase reaction buffer (50 m m Tris, pH 7.5, 150 m m NaCl, 10 m m CaCl2 ) for 24 h at 37 °C.

Article Title: Identification of host cell factors required for intoxication through use of modified cholera toxin
Article Snippet: .. We were unable to observe the Alexa Fluor 647 peptide with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer, so we inferred the molecular weight and activity as a nucleophile by setting up a test transpeptidation reaction with sortase A and an LPETG-tagged GFP substrate as described previously , then observing the mass change in the transpeptidation product by electrospray ionization mass spectrometry on a liquid chromatography mass spectrometer (Micromass MS Technologies), an HPLC system equipped with an HTC PAL autosampler (Paradigm MG4; Michrom BioResources), and a Symmetry 5 mm C8 column (2.1 × 50 mm, MeCN/H2O (0.1% formic acid) gradient mobile phase, 150 ml/min; Waters). .. The predicted molecular weight for the Alexa Fluor 647 peptide is 1,155.06 g/mol, obs = 1155.0 g/mol.

Activity Assay:

Article Title: Identification of host cell factors required for intoxication through use of modified cholera toxin
Article Snippet: .. We were unable to observe the Alexa Fluor 647 peptide with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer, so we inferred the molecular weight and activity as a nucleophile by setting up a test transpeptidation reaction with sortase A and an LPETG-tagged GFP substrate as described previously , then observing the mass change in the transpeptidation product by electrospray ionization mass spectrometry on a liquid chromatography mass spectrometer (Micromass MS Technologies), an HPLC system equipped with an HTC PAL autosampler (Paradigm MG4; Michrom BioResources), and a Symmetry 5 mm C8 column (2.1 × 50 mm, MeCN/H2O (0.1% formic acid) gradient mobile phase, 150 ml/min; Waters). .. The predicted molecular weight for the Alexa Fluor 647 peptide is 1,155.06 g/mol, obs = 1155.0 g/mol.

Liquid Chromatography:

Article Title: Substrate Filtering by the Active Site Crossover Loop in UCHL3 Revealed by Sortagging and Gain-of-function Mutations
Article Snippet: .. Mass Spectrometry —Liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) was performed on a Micromass LCT mass spectrometer (Micromass MS Technologies) and a Paradigm MG4 HPLC system equipped with a HTC PAL autosampler (Michrom BioResources) and a Waters Symmetry 5 μ m C8 column (2.1 × 50 mm, MeCN:H2 O (0.1% formic acid) gradient mobile phase, 150 μl/min). .. Cloning and Protein Expression —Sortase A was expressed and purified as described ( ).

Article Title: Identification of host cell factors required for intoxication through use of modified cholera toxin
Article Snippet: .. We were unable to observe the Alexa Fluor 647 peptide with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer, so we inferred the molecular weight and activity as a nucleophile by setting up a test transpeptidation reaction with sortase A and an LPETG-tagged GFP substrate as described previously , then observing the mass change in the transpeptidation product by electrospray ionization mass spectrometry on a liquid chromatography mass spectrometer (Micromass MS Technologies), an HPLC system equipped with an HTC PAL autosampler (Paradigm MG4; Michrom BioResources), and a Symmetry 5 mm C8 column (2.1 × 50 mm, MeCN/H2O (0.1% formic acid) gradient mobile phase, 150 ml/min; Waters). .. The predicted molecular weight for the Alexa Fluor 647 peptide is 1,155.06 g/mol, obs = 1155.0 g/mol.

Molecular Weight:

Article Title: Identification of host cell factors required for intoxication through use of modified cholera toxin
Article Snippet: .. We were unable to observe the Alexa Fluor 647 peptide with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer, so we inferred the molecular weight and activity as a nucleophile by setting up a test transpeptidation reaction with sortase A and an LPETG-tagged GFP substrate as described previously , then observing the mass change in the transpeptidation product by electrospray ionization mass spectrometry on a liquid chromatography mass spectrometer (Micromass MS Technologies), an HPLC system equipped with an HTC PAL autosampler (Paradigm MG4; Michrom BioResources), and a Symmetry 5 mm C8 column (2.1 × 50 mm, MeCN/H2O (0.1% formic acid) gradient mobile phase, 150 ml/min; Waters). .. The predicted molecular weight for the Alexa Fluor 647 peptide is 1,155.06 g/mol, obs = 1155.0 g/mol.

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    Michrom htc pal autosampler
    Htc Pal Autosampler, supplied by Michrom, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htc pal autosampler/product/Michrom
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    htc pal autosampler - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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