hrp streptavidin reaction  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 78

    Structured Review

    Thermo Fisher hrp streptavidin reaction
    Aging-associated change on ER expression and DNA methylation. ( A ) mRNA expression for ERα and ERβ in mice aorta normalized to the 18S expression. ( B ) Image on top shows a representative experiment of biotin-dCTP incorporation to DNA following no digestion (Mock) and digestion with MspI (no methylation sensitive) or HpaII (methylation sensitive).DNA was spotted in a nylon membrane and visualized with <t>streptavidin-HRP</t> method (data shown in triplicate). Bar graphs show (left) the results of densitometric analyses from pooled data for global DNA methylation and (right) percentage of specific DNA methylation at 5′ flanking region of the gene encoding ERα. OVX, ovariectomized; OVX+E2, ovariectomized treated with estrogen. Each data represents the mean ± SEM derived from 6–8 independent experiments. * p
    Hrp Streptavidin Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp streptavidin reaction/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp streptavidin reaction - by Bioz Stars, 2020-03
    78/100 stars

    Images

    1) Product Images from "Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ER?/ER? Balance in Female Mice"

    Article Title: Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ER?/ER? Balance in Female Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0025335

    Aging-associated change on ER expression and DNA methylation. ( A ) mRNA expression for ERα and ERβ in mice aorta normalized to the 18S expression. ( B ) Image on top shows a representative experiment of biotin-dCTP incorporation to DNA following no digestion (Mock) and digestion with MspI (no methylation sensitive) or HpaII (methylation sensitive).DNA was spotted in a nylon membrane and visualized with streptavidin-HRP method (data shown in triplicate). Bar graphs show (left) the results of densitometric analyses from pooled data for global DNA methylation and (right) percentage of specific DNA methylation at 5′ flanking region of the gene encoding ERα. OVX, ovariectomized; OVX+E2, ovariectomized treated with estrogen. Each data represents the mean ± SEM derived from 6–8 independent experiments. * p
    Figure Legend Snippet: Aging-associated change on ER expression and DNA methylation. ( A ) mRNA expression for ERα and ERβ in mice aorta normalized to the 18S expression. ( B ) Image on top shows a representative experiment of biotin-dCTP incorporation to DNA following no digestion (Mock) and digestion with MspI (no methylation sensitive) or HpaII (methylation sensitive).DNA was spotted in a nylon membrane and visualized with streptavidin-HRP method (data shown in triplicate). Bar graphs show (left) the results of densitometric analyses from pooled data for global DNA methylation and (right) percentage of specific DNA methylation at 5′ flanking region of the gene encoding ERα. OVX, ovariectomized; OVX+E2, ovariectomized treated with estrogen. Each data represents the mean ± SEM derived from 6–8 independent experiments. * p

    Techniques Used: Expressing, DNA Methylation Assay, Mouse Assay, Methylation, Derivative Assay

    Related Articles

    Isolation:

    Article Title: Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ER?/ER? Balance in Female Mice
    Article Snippet: Briefly, equal amounts of genomic DNA (500 ng), isolated from total aorta underwent no digestion (Mock) or digestion with 10 U of methylation-sensitive restriction enzyme HpaII or its isoschizomer MspI (New England Biolabs) for 16–18 hours in separate tubes. .. After washing with 0.4 N NaOH and cross-link at U.V. light, biotin incorporated to the membranes was visualized with HRP-streptavidin reaction (Pierce), according to manufacturer.

    Methylation:

    Article Title: Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ER?/ER? Balance in Female Mice
    Article Snippet: Paragraph title: Analysis of Global Methylation ... After washing with 0.4 N NaOH and cross-link at U.V. light, biotin incorporated to the membranes was visualized with HRP-streptavidin reaction (Pierce), according to manufacturer.

    Non-Isotopic Labeling:

    Article Title: Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ER?/ER? Balance in Female Mice
    Article Snippet: Analysis of Global Methylation Global DNA methylation status was determined by non-isotopic cytosine extension assay, as previously described . .. After washing with 0.4 N NaOH and cross-link at U.V. light, biotin incorporated to the membranes was visualized with HRP-streptavidin reaction (Pierce), according to manufacturer.

    DNA Methylation Assay:

    Article Title: Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ER?/ER? Balance in Female Mice
    Article Snippet: Analysis of Global Methylation Global DNA methylation status was determined by non-isotopic cytosine extension assay, as previously described . .. After washing with 0.4 N NaOH and cross-link at U.V. light, biotin incorporated to the membranes was visualized with HRP-streptavidin reaction (Pierce), according to manufacturer.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Thermo Fisher streptavidin conjugated to hrp
    TRAF6-mediated GSK3β ubiquitination at lysine 183 is critical for TLR3-dependent cytokine production. ( a ) BMDMs were stimulated with 10 μg ml −1 poly I:C for 10 min and subjected to immunoprecipitation with an anti-Ub antibody followed by western blotting with an anti-GSK3β antibody. ( b ) HEK293T cells transfected with HA-GSK3β and HA-Ub along with Flag-TRAF6 plasmids were subjected to immunoprecipitation with an anti-GSK3β antibody followed by western blotting with an anti-HA antibody. ( c ) HEK293T cells were transfected with HA-GSK3β and HA-Ub along with TRAF6 (WT) or TRAF6 (C70A) plasmids. These experiments were performed as described in b . ( d ) Traf6 +/+ and Traf6 −/− 3T3 cells stimulated with 10 μg ml −1 poly I:C for 10 min were subjected to immunoprecipitation with an anti-GSK3β antibody followed by western blotting with an anti-Ub antibody. ( e ) GSK3β proteins were incubated with E1, E2 and biotinylated-Ub (Bt-Ub) in the presence or absence of Flag-TRAF6 proteins for in vitro ubiquitination of GSK3β. Ubiquitination of GSK3β was analysed by western blotting with <t>streptavidin-HRP.</t> ( f ) HEK293T cells transfected with Ub and Flag-TRAF6 along with HA-GSK3β WT or various HA-GSK3β mutants were subjected to immunoprecipitation with an anti-HA antibody followed by western blotting with an anti-Ub antibody. ( g ) HEK293-TLR3 cells were transiently transfected with GSK3β (WT) or GSK3β (K183R) plasmids. The levels of IL-6, TNF-α and c-Fos mRNA were determined by real-time PCR analysis (top). GSK3β expression levels were confirmed by western blotting with an anti-HA antibody (bottom). A longer exposure of the HA blot shows the presence of ubiquitin ladder. Data are presented as the mean±s.d. from at least three independent experiments. Statistical analyses were calculated using the Student’s t -test (** P
    Streptavidin Conjugated To Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin conjugated to hrp/product/Thermo Fisher
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    streptavidin conjugated to hrp - by Bioz Stars, 2020-03
    91/100 stars
      Buy from Supplier

    99
    Thermo Fisher streptavidin horseradish peroxidase hrp
    Deletion of Acaca in beta cells impairs glucose-driven lipogenesis in mice. ( a ) PCR analysis to confirm deletion of the floxed Acaca allele (indicated by arrow) in Ins2 cre- Acaca flox/flox (βACC1KO) but not in Acaca flox/flox (ACC1 flox) mice. A control PCR confirmed amplifiable DNA template in all samples (upper band). ( b ) ACC1 protein in islet lysates assessed by avidin pull-down and <t>streptavidin–HRP</t> detection. Pyruvate carboxylase (PC) was used as a loading control. n =3 batches of islets from independent mice. ( c – e ) A d -[U- 14 C]glucose tracer was used to measure glucose-driven metabolic flux in islets isolated from mice and incorporation into cellular lipid pools ( c ), aqueous metabolites ( d ) and glucose oxidation ( e ) were quantified. White bars, INS2cre mouse islets; grey bars, βACC1KO mouse islets. n =4 batches of islets from independent mice. Data are presented as mean ± SEM. * p
    Streptavidin Horseradish Peroxidase Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin horseradish peroxidase hrp/product/Thermo Fisher
    Average 99 stars, based on 171 article reviews
    Price from $9.99 to $1999.99
    streptavidin horseradish peroxidase hrp - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    TRAF6-mediated GSK3β ubiquitination at lysine 183 is critical for TLR3-dependent cytokine production. ( a ) BMDMs were stimulated with 10 μg ml −1 poly I:C for 10 min and subjected to immunoprecipitation with an anti-Ub antibody followed by western blotting with an anti-GSK3β antibody. ( b ) HEK293T cells transfected with HA-GSK3β and HA-Ub along with Flag-TRAF6 plasmids were subjected to immunoprecipitation with an anti-GSK3β antibody followed by western blotting with an anti-HA antibody. ( c ) HEK293T cells were transfected with HA-GSK3β and HA-Ub along with TRAF6 (WT) or TRAF6 (C70A) plasmids. These experiments were performed as described in b . ( d ) Traf6 +/+ and Traf6 −/− 3T3 cells stimulated with 10 μg ml −1 poly I:C for 10 min were subjected to immunoprecipitation with an anti-GSK3β antibody followed by western blotting with an anti-Ub antibody. ( e ) GSK3β proteins were incubated with E1, E2 and biotinylated-Ub (Bt-Ub) in the presence or absence of Flag-TRAF6 proteins for in vitro ubiquitination of GSK3β. Ubiquitination of GSK3β was analysed by western blotting with streptavidin-HRP. ( f ) HEK293T cells transfected with Ub and Flag-TRAF6 along with HA-GSK3β WT or various HA-GSK3β mutants were subjected to immunoprecipitation with an anti-HA antibody followed by western blotting with an anti-Ub antibody. ( g ) HEK293-TLR3 cells were transiently transfected with GSK3β (WT) or GSK3β (K183R) plasmids. The levels of IL-6, TNF-α and c-Fos mRNA were determined by real-time PCR analysis (top). GSK3β expression levels were confirmed by western blotting with an anti-HA antibody (bottom). A longer exposure of the HA blot shows the presence of ubiquitin ladder. Data are presented as the mean±s.d. from at least three independent experiments. Statistical analyses were calculated using the Student’s t -test (** P

    Journal: Nature Communications

    Article Title: Glycogen synthase kinase 3β ubiquitination by TRAF6 regulates TLR3-mediated pro-inflammatory cytokine production

    doi: 10.1038/ncomms7765

    Figure Lengend Snippet: TRAF6-mediated GSK3β ubiquitination at lysine 183 is critical for TLR3-dependent cytokine production. ( a ) BMDMs were stimulated with 10 μg ml −1 poly I:C for 10 min and subjected to immunoprecipitation with an anti-Ub antibody followed by western blotting with an anti-GSK3β antibody. ( b ) HEK293T cells transfected with HA-GSK3β and HA-Ub along with Flag-TRAF6 plasmids were subjected to immunoprecipitation with an anti-GSK3β antibody followed by western blotting with an anti-HA antibody. ( c ) HEK293T cells were transfected with HA-GSK3β and HA-Ub along with TRAF6 (WT) or TRAF6 (C70A) plasmids. These experiments were performed as described in b . ( d ) Traf6 +/+ and Traf6 −/− 3T3 cells stimulated with 10 μg ml −1 poly I:C for 10 min were subjected to immunoprecipitation with an anti-GSK3β antibody followed by western blotting with an anti-Ub antibody. ( e ) GSK3β proteins were incubated with E1, E2 and biotinylated-Ub (Bt-Ub) in the presence or absence of Flag-TRAF6 proteins for in vitro ubiquitination of GSK3β. Ubiquitination of GSK3β was analysed by western blotting with streptavidin-HRP. ( f ) HEK293T cells transfected with Ub and Flag-TRAF6 along with HA-GSK3β WT or various HA-GSK3β mutants were subjected to immunoprecipitation with an anti-HA antibody followed by western blotting with an anti-Ub antibody. ( g ) HEK293-TLR3 cells were transiently transfected with GSK3β (WT) or GSK3β (K183R) plasmids. The levels of IL-6, TNF-α and c-Fos mRNA were determined by real-time PCR analysis (top). GSK3β expression levels were confirmed by western blotting with an anti-HA antibody (bottom). A longer exposure of the HA blot shows the presence of ubiquitin ladder. Data are presented as the mean±s.d. from at least three independent experiments. Statistical analyses were calculated using the Student’s t -test (** P

    Article Snippet: Samples were subsequently immunoprecipitated with an anti-GSK3β antibody and separated on SDS–PAGE followed by streptavidin conjugated to HRP (Thermo Fisher Scientific).

    Techniques: Immunoprecipitation, Western Blot, Transfection, Incubation, In Vitro, Real-time Polymerase Chain Reaction, Expressing

    Deletion of Acaca in beta cells impairs glucose-driven lipogenesis in mice. ( a ) PCR analysis to confirm deletion of the floxed Acaca allele (indicated by arrow) in Ins2 cre- Acaca flox/flox (βACC1KO) but not in Acaca flox/flox (ACC1 flox) mice. A control PCR confirmed amplifiable DNA template in all samples (upper band). ( b ) ACC1 protein in islet lysates assessed by avidin pull-down and streptavidin–HRP detection. Pyruvate carboxylase (PC) was used as a loading control. n =3 batches of islets from independent mice. ( c – e ) A d -[U- 14 C]glucose tracer was used to measure glucose-driven metabolic flux in islets isolated from mice and incorporation into cellular lipid pools ( c ), aqueous metabolites ( d ) and glucose oxidation ( e ) were quantified. White bars, INS2cre mouse islets; grey bars, βACC1KO mouse islets. n =4 batches of islets from independent mice. Data are presented as mean ± SEM. * p

    Journal: Diabetologia

    Article Title: Disruption of beta cell acetyl-CoA carboxylase-1 in mice impairs insulin secretion and beta cell mass

    doi: 10.1007/s00125-018-4743-7

    Figure Lengend Snippet: Deletion of Acaca in beta cells impairs glucose-driven lipogenesis in mice. ( a ) PCR analysis to confirm deletion of the floxed Acaca allele (indicated by arrow) in Ins2 cre- Acaca flox/flox (βACC1KO) but not in Acaca flox/flox (ACC1 flox) mice. A control PCR confirmed amplifiable DNA template in all samples (upper band). ( b ) ACC1 protein in islet lysates assessed by avidin pull-down and streptavidin–HRP detection. Pyruvate carboxylase (PC) was used as a loading control. n =3 batches of islets from independent mice. ( c – e ) A d -[U- 14 C]glucose tracer was used to measure glucose-driven metabolic flux in islets isolated from mice and incorporation into cellular lipid pools ( c ), aqueous metabolites ( d ) and glucose oxidation ( e ) were quantified. White bars, INS2cre mouse islets; grey bars, βACC1KO mouse islets. n =4 batches of islets from independent mice. Data are presented as mean ± SEM. * p

    Article Snippet: ACC1 protein was identified by avidin–agarose bead pull-down, SDS-PAGE, transfer to polyvinylidene difluoride (PVDF), incubation with streptavidin–horseradish peroxidase (HRP) (ThermoFisher) and chemiluminescent detection: this method utilises the affinity of the endogenous ACC1-bound biotin for avidin/streptavidin and has been described previously [ ].

    Techniques: Mouse Assay, Polymerase Chain Reaction, Avidin-Biotin Assay, Isolation

    Identification of genome-wide association of Klf9 in HT22 cell chromatin using chromatin-streptavidin precipitation sequencing. a HT22 [BirA/FLBIO-Klf9] cells express biotinylated Klf9. Whole cell extracts from the HT22 parent cell line (lane 1), HT22 [BirA] cells (lane 2) or HT22 [BirA/FLBIO-Klf9] (lane 3) were fractionated on 10% SDS-PAGE and analyzed by Western blotting using streptavidin-HRP. b Chromatin-streptavidin precipitation gives ~25-fold enrichment at the Klf13 promoter in HT22 [BirA/FLBIO-Klf9] cells compared with HT22 [BirA] cells. Precipitated DNA was analyzed by qPCR at the Klf13 promoter and intron (negative control region). The asterisk indicates a statistically significant difference by Student’s two-sample t -test ( p

    Journal: BMC Genomics

    Article Title: The Krüppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding

    doi: 10.1186/s12864-017-3640-7

    Figure Lengend Snippet: Identification of genome-wide association of Klf9 in HT22 cell chromatin using chromatin-streptavidin precipitation sequencing. a HT22 [BirA/FLBIO-Klf9] cells express biotinylated Klf9. Whole cell extracts from the HT22 parent cell line (lane 1), HT22 [BirA] cells (lane 2) or HT22 [BirA/FLBIO-Klf9] (lane 3) were fractionated on 10% SDS-PAGE and analyzed by Western blotting using streptavidin-HRP. b Chromatin-streptavidin precipitation gives ~25-fold enrichment at the Klf13 promoter in HT22 [BirA/FLBIO-Klf9] cells compared with HT22 [BirA] cells. Precipitated DNA was analyzed by qPCR at the Klf13 promoter and intron (negative control region). The asterisk indicates a statistically significant difference by Student’s two-sample t -test ( p

    Article Snippet: To detect FLBIO-Klf9 we incubated the membrane with Streptavidin-HRP (Thermo; diluted 1:1000) for 1 h. Immune or streptavidin-HRP complexes were revealed by chemiluminescence detection using Pierce ECL Substrate (ThermoFisher Scientific).

    Techniques: GWAS, Sequencing, SDS Page, Western Blot, Real-time Polymerase Chain Reaction, Negative Control