hpy 188iii endonucleases  (New England Biolabs)


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  • 94
    Name:
    Hpy188III
    Description:
    Hpy188III 2 500 units
    Catalog Number:
    r0622l
    Price:
    282
    Size:
    2 500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs hpy 188iii endonucleases
    Hpy188III
    Hpy188III 2 500 units
    https://www.bioz.com/result/hpy 188iii endonucleases/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpy 188iii endonucleases - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Combined Bisulfite Restriction Analysis Assay:

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel. .. After staining with ethidium bromide, the image was visualized under UV illumination.

    Amplification:

    Article Title: Bi-allelic MCM10 mutations cause telomere shortening with immune dysfunction and cardiomyopathy
    Article Snippet: .. Subcloned cells were screened for correct targeting by PCR amplification and restriction enzyme digestion (MCM10 exon 3, Hpy199III (NEB R0622); CDC45 exon 3, PflMI (NEB R0509), XcmI (NEB R0533) or AlwNI (NEB R0514); MCM4 exon 2, BglI (NEB R0143). .. Specific mutations were identified by PCR and Illumina sequencing or PCR, DNA sequencing and TIDE ( T racking of Indels by De composition) analyses .

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. We amplified a sequence that contains SNP3 from somatic cell hybrid (SCH) and genomic DNAs and digested aliquots of the PCR products with Hpy188I or Hpy188III. .. SCH DNAs were completely sensitive to Hpy188I and resistant to Hpy188III or vice versa, as expected ( and data not shown).

    Agarose Gel Electrophoresis:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

    Methylation:

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel. .. After staining with ethidium bromide, the image was visualized under UV illumination.

    Purification:

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel. .. After staining with ethidium bromide, the image was visualized under UV illumination.

    Polymerase Chain Reaction:

    Article Title: Bi-allelic MCM10 mutations cause telomere shortening with immune dysfunction and cardiomyopathy
    Article Snippet: .. Subcloned cells were screened for correct targeting by PCR amplification and restriction enzyme digestion (MCM10 exon 3, Hpy199III (NEB R0622); CDC45 exon 3, PflMI (NEB R0509), XcmI (NEB R0533) or AlwNI (NEB R0514); MCM4 exon 2, BglI (NEB R0143). .. Specific mutations were identified by PCR and Illumina sequencing or PCR, DNA sequencing and TIDE ( T racking of Indels by De composition) analyses .

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. We amplified a sequence that contains SNP3 from somatic cell hybrid (SCH) and genomic DNAs and digested aliquots of the PCR products with Hpy188I or Hpy188III. .. SCH DNAs were completely sensitive to Hpy188I and resistant to Hpy188III or vice versa, as expected ( and data not shown).

    Incubation:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

    other:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Of the 11 examined FSHD DNA samples, all had at least one 4qA161 allele, as seen by the undigested band in the Hpy188I digest and a digested band in the Hpy188III digest ( ).

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: SCH DNAs were completely sensitive to Hpy188I and resistant to Hpy188III or vice versa, as expected ( and data not shown).

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: To analyse the association of the 4qA161 haplotype with FSHD, we identified a SNP ( , SNP3) located 307 bp proximal to D4Z4 (GenBank , nt 4519) at overlapping recognition sites for Hpy188I and Hpy188III ( ).

    Sequencing:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. We amplified a sequence that contains SNP3 from somatic cell hybrid (SCH) and genomic DNAs and digested aliquots of the PCR products with Hpy188I or Hpy188III. .. SCH DNAs were completely sensitive to Hpy188I and resistant to Hpy188III or vice versa, as expected ( and data not shown).

    Staining:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

    Plasmid Preparation:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

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  • News
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  • Team
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  • 94
    New England Biolabs hpy 188iii endonucleases
    Hpy 188iii Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpy 188iii endonucleases/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpy 188iii endonucleases - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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